FOOD CHEMISTRY
·
2025
·
IF 9.8
Xiaojie Zhang,Zixuan Xu,Zichen Yun,Lulu Bai,Xiaohui Xu,Qifei Luo,Dorjgoo Purevtsogt,Lan Yang,Dacheng Liu
This study aims to establish a detection method based on enzymatic hydrolysis combined with high-performance liquid chromatography (HPLC) technology for the quantitative analysis of yeast β-glucan content in composite yeast cultures. The method employs exo-1,3-β-glucanase plus β-glucosidase (A1 enzyme) to enzymatically hydrolyze total glucans in the sample. Amyloglucosidase plus invertase (B1 enzyme) along with trehalase (B2 enzyme) are used to enzymatically hydrolyze α-glucans in the sample, and purified lichenase (C1 enzyme) and β-glucosidase (C2 enzyme) to hydrolyze the cereal β-glucans in the sample. The glucose produced after hydrolysis is subjected to PMP derivatization and detected by HPLC. By applying the formula, the results of the three types of glucan content were obtained, namely X A , X B , and X C . Ultimately, the content of yeast β-glucan in the composite yeast culture was calculated as X A − X B − X C . The experimental results showed that glucose exhibited good linearity within the concentration range of 1-400 μg/mL, with a correlation range of 0.99999, a detection limit of 0.084 mg/L, a quantification limit of 0.280 mg/L, an average spiked recovery rate of 80.857 % to 105.456 %, and an RSD of less than 5 %. These data indicate that the method established in this study has high precision and good feasibility, making it suitable for the detection and analysis of yeast β-glucan in composite yeast cultures.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2025
·
IF 5.6
Xiao-Nan Zhou,Mu-Lun Wu,Xu Hao,Jia-Xin Li,Yu Song,Biao Zhang,Zhi-Lei Wei,Jian-Feng Yang,Bo Wang,Kozo Ishizaki
Hollow spherical SiC ceramics exhibit significant potential as multifunctional and structural materials. Herein, the β-SiC hollow spheres were synthesized by in-situ vapor-solid reaction (carbothermal reduction) between mesocarbon microbeads (MCMB) and SiO vapor generated from SiO powders, and subsequent removal of the residual carbon cores by oxidization. The diameter of SiC hollow spheres accurately inherits the size of pristine MCMB, demonstrating a shape memory effect. Meanwhile, the shell thickness of SiC hollow sphere can be autonomously regulated from 0.46 to 3.87 μm by adjusting the siliconization degree (corresponding to the content of SiO). In addition, a variety of characterizations were used to describe the phase and structure of SiC hollow spheres, confirming their high-purity. The SiC hollow spheres also exhibit a high specific surface area of 4.21 m 2 g −1 and good thermal stability. This technique presents a new paradigm in the preparation of hollow spheres.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
·
2025
·Vol. 29
·期 7
·e70539
·
IF 4.2
Tianyi Ji,Xiangying Li,Jiachen Li,Guan Wang
Wnt signalling is a highly conserved signalling pathway that plays an important role in a variety of biological processes. Frizzled (Fzd) family proteins are receptors for Wnt ligands. The physiological processes involved in mature trafficking of Fzd proteins remain elusive. Here, we identified asparagine residues 59 and 144 as the N-glycosylation modification sites of Fzd4. Sequence analysis of Fzd4 in different species showed that the two asparagine residues were highly conserved. N-glycosylation modification of Fzd4 is indispensable for its maturation and transport to the plasma membrane. N-glycosylation modification enhances the stability of Fzd4 and is also necessary for Fzd4 activity, which promotes Fzd4 interaction with Wnt ligands and co-receptor Norrin. Knockout of Fzd4 in the non-small cell lung cancer (NSCLC) cell line A549 followed by replenishment of Fzd4 glycosylation site mutants inhibited the growth and migration ability of A549 cells in vitro and in vivo. In summary, we identified N-glycosylation modification sites of Fzd4. N-glycosylation modification of Fzd4 is necessary for its stability and activity. When N-glycosylation modification is absent, Fzd4 cannot mediate the Wnt/β-catenin signalling pathway, which can inhibit the proliferation and migration of NSCLC and provide new targets and strategies for the treatment of NSCLC.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Tinglan Yuan,Ting Ye,Haitao Guo,Yaoruixi Yang,Gongshuai Song,Danli Wang,Ling Li,Yong Cheng,Jinyan Gong
Complexes formed between polyphenols and proteins could be used as a good alternative to improve the phytochemical stability in food matrices. While interactions between tea catechins and milk proteins are prevalent in food systems, the effects of different structures of catechins on these interactions, and the resulting alterations in structural and functional properties of covalent complexes formed remain poorly understood. This study investigated the effect of different catechin structures on covalent interactions with β-lactoglobulin (β-Lg). (+)-Catechin (C), (−)-epicatechin (EC), (−)-epigallocatechin (EGC), (−)-epicatechin gallate (ECG), (−)-gallocatechin gallate (GCG), and (−)-epigallocatechin gallate (EGCG) were covalently bound to β-Lg through the alkaline method. β-Lg-catechin complexes had lower fluorescence intensity, hydrophobicity, α-helix content, free amino groups, and sulfhydryl groups than native β-Lg. Binding to catechins (especially ECG, GCG, and EGCG) significantly enhanced β-Lg antioxidant activity, emulsification activity, and stability. Furthermore, β-Lg-catechin complexes substantially enhance emulsion freeze-thaw, thermal stability, storage stability, and oxidative stability. Galloylated catechins exhibited the greatest graft affinity towards β-Lg, and their complexes demonstrated far superior antioxidant activity and stability than non-galloylated catechins. In conclusion, customized β-Lg-catechin conjugates have considerable potential as carriers in food that can enhance emulsion phytochemical stability.
✓ 已验证
产品关联
DOI
高影响因子
APPLIED ORGANOMETALLIC CHEMISTRY
·
2025
·Vol. 39
·期 6
·e70185
·
IF 0
Zhiyuan Li,Yanan Dai,Lishuang Shi,Weihao Wu,Hongyan Xie,Zehuai Mou
Trifluoromethyl-containing β-diketiminate ligands (CF 3 )BDI Et -H = (2,6-di-Et 2 C 6 H 3 )N=C (CF 3 )CH=C (CF 3 )NH(2,6-di-Et 2 C 6 H 3 ), (CF 3 )BDI i Pr -H = (2,6-di- i Pr 2 C 6 H 3 )N=C (CF 3 )CH=C (CF 3 )NH(2,6-di- i Pr 2 C 6 H 3 ) react with Mg n Bu 2 readily to provide THF-ligated four-coordinate monomeric magnesium butyl complexes (CF 3 )BDI Et Mg n Bu (THF) ( 1 ) and (CF 3 )BDI i Pr Mg n Bu (THF) ( 2 ) in THF, and three-coordinate monomeric complexes (CF 3 )BDI Et Mg n Bu ( 3 ) and (CF 3 )BDI i Pr Mg n Bu ( 4 ) in toluene, respectively. The alkane elimination between 4 and NH (SiHMe 2 ) 2 gives silylamido complex 5 in toluene. Treatments of 4 with heterocumulene tert-butyl isothiocyanate, tert-butyl isocyanate, and N,N′-diisopropylcarbodiimide produce single insertion derivatives (CF 3 )BDI i Pr MgN ( t Bu)C ( n Bu)S ( 6 ), (CF 3 )BDI i Pr MgN ( t Bu)C ( n Bu)O ( 7 ), and (CF 3 )BDI i Pr MgN ( i Pr)C ( n Bu)N ( i Pr) ( 8 ), respectively. All these new compounds were characterized by NMR spectroscopy, and the molecular structures of complexes 1 , 2 , 4 – 7 were further confirmed by single-crystal X-ray diffraction analyses. Moreover, representative complexes 2 – 5 were employed as pre-catalysts for the hydroboration of commercially available carbodiimides bearing aliphatic and aromatic substituents with pinacol borane HBpin, including diisopropylcarbodiimide (DIC), dicyclohexylcarbodiimide (DCC), di- tert -butylcarbodiimide, 2,6-diisopropylphenylcarbodiimide, and unsymmetric 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDCI), in which the three-coordinate monomeric complexes 3 and 4 showed better efficiency giving corresponding N -boryl formamidine products in high yields. A possible mechanistic pathway of the hydroboration reaction has been proposed.
✓ 已验证
产品关联
DOI
REACTIVE & FUNCTIONAL POLYMERS
·
2025
·
IF 5.1
Simin Huang,Yizhong Yuan,Huimei Yu,Jinyu Sun,Xiaohui Tian,Chunhua Cai,Yuxuan Han,Yao Zhang
The remediation of wastewater contaminated with heavy metals remains a serious challenge due to the continuing threats posed to ecosystems and human health. While conventional methods such as membrane filtration and flocculation exist, developing sustainable adsorbents with high capacity and reusability is imperative. Therefore, we propose a novel strategy to overcome the limitations of natural polymer-based hydrogels by integrating β-FeOOH nanoparticles into sodium alginate matrices. FTIR, XPS and TG reveal that the incorporation of β-FeOOH not only increases the adsorption active sites but also improves the thermal stability of the composite hydrogels. The composite hydrogel exhibits exceptional adsorption capacity (581.40 mg/g). Crucially, the material maintains over 85 % of its initial efficiency after five adsorption-desorption cycles, demonstrating strong structural integrity and practical applicability. This work provides a mechanistic framework for designing multifunctional hydrogels through nanomaterial hybridization, offering a sustainable pathway for water purification technologies.
✓ 已验证
DOI
高影响因子
Journal of Cleaner Production
·
2024
·
IF 9.7
Ting Yao,Hong Chen,Yue Luo,Hui Li,Xiaolan Shao,Qianqi Zheng,Dingdi Tu,Bei Yan,Jinfeng Dai,Lianyang Bai,Kailin Liu
Herbicides are the primary pesticides that can easily pollute water bodies, attracting significant attention from researchers regarding the effective removal of herbicide residues from the water environment. In this study, a β-cyclodextrin-reduced graphene oxide (β-CD-BTCA-rGO) aerogel was prepared following the self-assembly hydrothermal method using 1,2,3,4-butane tetracarboxylic acid (BTCA) as crosslinkers. The self-assembly process involved the cross-linking of “soft” β-cyclodextrin (β-CD) with “hard” graphene oxide (GO) nanosheets, forming a stable β-CD-BTCA-rGO aerogel. The prepared β-CD-BTCA-rGO aerogel demonstrated excellent adsorption performance. The adsorption capacities were 62.3 and 46.8 mg/g for sulfentrazone and quinclorac, respectively, surpassing the traditional reduced graphene oxide (rGO) aerogel and citric acid cross-linked (β-CD-CA-rGO) aerogel. Correspondingly, its adsorption capacity for quinclorac was 1.8 times and 12 times that of rGO aerogel and β-CD-CA-rGO aerogel, respectively. Quantum chemical calculations at the density functional theory (DFT) level, utilizing the Fukui function to predict chemical reaction sites, indicated that BTCA, as crosslinkers, formed stable chemical bonds (ester bonds) with GO through hydroxyl and carboxyl surface functional groups, leading to the formation of stable porous aerogels. Furthermore, the highest occupied molecular orbital-lowest unoccupied molecular orbital (HOMO-LUMO) energy gap was quantitatively measured for various adsorption mechanisms, revealing that electrostatic adsorption was the main force of the adsorption on the aerogels. The β-CD-BTCA-rGO aerogels prepared in this study demonstrated economic feasibility as adsorbents and exhibited good reproducibility. Consequently, β-CD-BTCA-rGO aerogels hold promising application prospects in the remediation of pesticide pollution in water systems.
✓ 已验证
产品关联
DOI
高影响因子
BIOORGANIC CHEMISTRY
·
2020
·
IF 4.831
De-Yun Cui,Yi Yang,Meng-Meng Bai,Jiang-Xue Han,Cong-Cong Wang,Hong-Tao Kong,Bo-Yuan Shen,Da-Chao Yan,Chun-Ling Xiao,Yi-Shuang Liu,En Zhang
New Delhi Metallo-β-lactamase-1 (NDM-1), a Zn (II)-dependent enzyme, can catalyze the hydrolysis of almost all β-lactam antibiotics including carbapenems, resulting in bacterial antibiotic resistance, which threatens public health globally. Based on our finding that H 2 dedpa is as an efficient NDM-1 inhibitor, a series of H 2 dedpa derivatives was systematically prepared. These compounds exhibited significant activity against NDM-1, with IC 50 values 0.06–0.94 μM. In vitro , compounds 6k and 6n could restore the activity of meropenem against Klebsiella pneumoniae , Escherichia coli and Proteus mirabilis possessing either NDM or IMP. In particular, the activity of meropenem against E. coli producing NDM-4 could be improved up to 5333 times when these two compounds were used. Time–kill cell-based assays showed that 99.9% of P. mirabilis were killed when treated with meropenem in combination with compound 6k or 6n . Furthermore, compounds 6k and 6n were nonhemolytic (HC 50 > 1280 μg/mL) and showed low toxicity toward mammalian (HeLa) cells. Mechanistic studies indicated that compounds 6k and 6n inhibit NDM-1 by chelating the Zn 2+ ion of the enzyme.
✓ 已验证
DOI
高影响因子
CARBOHYDRATE RESEARCH
·
2018
·
IF 2.074
Zhiqiang Jiang,Xiaoyan Dong,Yan Sun
Amyloid β-protein (Aβ) aggregation is crucial for the pathogenesis of Alzheimer's disease, and surface charge of nanoparticles (NPs) has been recognized as an important factor influencing Aβ aggregation. Herein, we report a systematic study on the issue with a series of self-assembled chitosan-hyaluronic acid composite (CH) NPs of different surface charges (CH1 to CH7, zeta potentials from +38 to −35 mV). Both the positive and negative CH NPs inhibited Aβ aggregation and the inhibitory effect increased with increasing the surface charges density. Circular dichroism spectroscopy and atomic force microscopy revealed the difference in their working mechanisms. Studies at different pH values further confirmed the importance of electrostatic interactions in Aβ aggregation and presented that the effects of CH NPs changed due to the change of Aβ charge property with pH. This work has thus provided new insight into the surface charge effects on Aβ aggregation.
✓ 已验证
DOI
高影响因子
Nano Research
·
2020
·Vol. 13
·期 7
·1933-1942
·
IF 8.183
He Wenya,Ren Xiaoyan,Yan Zhiqiang,Wang Jin,Lu Lehui
The performance of water purification by adsorption method has been limited owing to the fact that most of current available adsorbents fail to achieve satisfactory removal performance for organic micropollutants. Herein, we report the design and synthesis of novel porous polymeric adsorbent built from β-cyclodextrin (β-CD), in which β-CD molecules are arranged in an ordered bis (β-CD) tubular assemblies. The induction of bis (β-CD) units renders them high adsorption affinity toward bisphenols (bisphenol A and its analogues bisphenol B, bisphenol F and bisphenol S), the typical endocrine disruptors, via the formation of stable host-guest inclusion complexes in aquatic systems. In combination with their high porosity (Brunauer-Emmett-Teller (BET) surface area of 150 m 2 ·g −1 ), abundant β-CD content and fast sorption kinetics, the obtained adsorbent outperforms commercial water purifier in elimination of bisphenol micropollutants from potable water. Our work may open a new avenue for designing highly efficient adsorbents for removal of organic micropollutants from aquatic systems.
✓ 已验证
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2020
·
IF 4.972
Li-Ping Peng,Chuan-He Tang
This work reports a kind of novel antioxidant Pickering high internal phase emulsions (HIPEs; with an oil fraction, ϕ > 0.74) stabilized by soy β -conglycinin ( β -CG) and polyphenol complex nanoparticles, as outstanding protective containers for lipophilic nutraceuticals. The nanoparticles with a representative polyphenol ((-)-epigallocatechin-3-gallate; EGCG) encapsulated were fabricated through an ethanol-mediated dissociation and re-assembly process of β -CG, with greater particle size and higher surface hydrophilicity observed at higher initial EGCG concentrations. Using these co-assembled nanoparticles as sole stabilizers, a kind of HIPE gels with similar gel stiffness and microstructure, could be easily fabricated at ϕ = 0.8 and a protein concentration in the aqueous phase of 1.0 wt% using polyunsatuated fatty acid-rich flaxseed oil as the dispersed phase. These HIPE gels were extraordinarily stable against heating or long-term storage, but susceptible to freeze-thawing. The as-fabricated HIPEs showed an excellent protection to β -carotene (encapsulated in oil phase) against heating, as well as an inhibition of lipid oxidation. The oxidation protection was in an EGCG concentration dependent manner. The results would be of interest for providing a novel strategy to fabricate functionalized Pickering HIPEs as potential containers or delivery systems for lipophilic nutraceuticals.
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2024
·
IF 5.4
Xianshuo Zhang,Peipei Wang,Xinsheng Wang,Yaoyu Xu,Taolin Cheng,Chengjie Zhang,Jiaying Ding,Yunfeng Shi,Wei Ma,Cui-Yun Yu,Hua Wei
Hyaluronic acid (HA)-based tumor microenvironment-responsive nanocontainers are attractive candidates for anticancer drug delivery due to HA's excellent biocompatibility , biodegradability, and CD44-targeting properties. Nevertheless, the consecutive synthesis of stabilized, stealthy, responsive HA-based multicomponent nanomedicines generally requires multi-step preparation and purification procedures, leading to batch-to-batch variation and scale-up difficulties. To develop a facile yet robust strategy for promoted translations, a silica monomer containing a cross-linkable diethoxysilyl unit was prepared to enable in situ crosslinking without any additives. Further combined with the host-guest inclusion complexation between β -cyclodextrin-grafted HA (HA-CD) and ferrocene-functionalized polymers, ferrocene-terminated poly(oligo(ethylene glycol) methyl ether methacrylate (Fc-POEGMA) and Fc-terminated poly( ε -caprolactone)- b -poly(3-(diethoxymethylsilyl)propyl(2-(methacryloyloxy)ethyl) carbamate) (Fc-PCL- b -PDESPMA), a reactive oxygen species (ROS)-sensitive supramolecular polymer construct, Fc-POEGMA/Fc-PCL- b -PDESPMA@HA-CD was readily fabricated to integrate stealthy POEGMA, tumor active targeting HA, and an in situ cross-linkable PDESPMA sequence. Supramolecular amphiphilic copolymers with two different POEGMA contents of 25 wt% ( P1 ) and 20 wt% ( P2 ) were prepared via a simple physical mixing process, affording two core-crosslinked (CCL) micelles via an in situ sol-gel process of ethoxysilyl groups. The P1 -based CCL micelles show not only desired colloidal stability against high dilution, but also an intracellular ROS-mimicking environment-induced particulate aggregation that is beneficial for promoted intracellular release of the loaded cargoes. Most importantly, P1 -based nanomedicines exhibited greater cytotoxicity in CD44 receptor-positive HeLa cells than that in CD44 receptor-negative MCF-7 cells. Overall, this work developed HA-based nanomedicines with sufficient extracellular colloidal stability and efficient intracellular destabilization properties for enhanced anticancer drug delivery via smart integration of in situ crosslinking and supramolecular complexation.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）