Heliyon
·
2024
·
IF 3.4
Kun Miao,Yawei Zhao,Ning Xue
The prevalence of obesity and its primary associated comorbidities, such as type 2 diabetes and fatty liver disease, has reached epidemic proportions, with no successful treatment available at present. Heat shock protein 90 (HSP90), a crucial chaperone, plays a key role in de novo lipogenesis (DNL) by stabilizing and maintaining sterol regulatory element binding protein (SREBP) activity. Kongensin A (KA), derived from Croton kongensis , inhibits RIP3-mediated necrosis, showing promise as an anti-necrotic and anti-inflammatory agent. It is not yet clear if KA, acting as an HSP90 inhibitor, can enhance hyperlipidemia, hepatic steatosis, and insulin resistance in obese individuals by controlling lipid metabolism. In this study, we first found that KA can potentially decrease lipid content at the cellular level. C57BL/6J mice were given a high-fat diet (HFD) and received KA and lovastatin through oral administration for 7 weeks. KA improved hyperlipidemia, fatty liver, and insulin resistance, as well as reduced body weight in diet-induced obese (DIO) mice, with no significant alteration in food intake. In vitro , KA suppressed DNL and reduced the amounts of mSREBPs. KA promoted mSREBP degradation via the FBW7-mediated ubiquitin-proteasome pathway. KA decreased the level of p -Akt Ser308, and p -GSK3β Ser9 by inhibiting the interaction between HSP90β and Akt. Overall, KA enhanced hyperlipidemia, hepatic steatosis, and insulin resistance by blocking SREBP activity, thereby impacting the FBW7-controlled ubiquitin-proteasome pathway.
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POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2024
·
IF 6.4
Yujie Liu,Xinyv Li,Chu Gong,Yonghong Cao,Jun Wang,Min Han,Jun-Li Yang
Anthracnose is a plant disease caused by Colletotrichum spp ., known for its widespread infectivity and extreme destructiveness. Colletotrichum gloeosporioides is a representative pathogen of anthracnose in China. Gypenosides GP4 - GP7 , derived from Gynostemma pentaphyllum (Thunb.) Makino, could significantly inhibit the growth of C. gloeosporioides mycelial, with EC 50 values of 96.98, 27.5, 38.48, and 61.59 mg L −1 . The inhibitory effect of these compounds surpassed the commonly used chemical pesticide chlorothalonant and plant-derived pesticide matrine. Among them, the most active compound GP5 also showed a significant inhibitory effect on spore germination and bud tube elongation of C. gloeosporioides . In addition, GP5 could effectively suppress the spread of anthracnose spots in postharvest fruit. Transmission electron microscopy and fluorescence microscopy demonstrated that GP5 primarily exerted its antifungal function by activating cellular autophagy. Additionally, proteomics analysis revealed that GP5 had an antifungal effect against C. gloeosporioides by enhancing cellular autophagy through upregulation of the expression of the autophagy-related protein Atg8. This study presents a novel approach for the control and management of anthracnose in C. gloeosporioides . Consequently, GP5 has the potential to be developed as a plant-derived fungicide for the biological control of anthracnose.
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BIOMACROMOLECULES
·
2025
·
IF 5.4
Hua Guo,Huimin Wang,Yiyi Zhang,Hong Deng,Ching-Hsuan Tung,Weiqi Zhang
The complex of doxorubicin (Dox) and intracellular iron in cardiomyocytes generates reactive oxygen species (ROS), contributing to commonly observed cardiotoxicity. To enhance the anticancer potency and minimize the cardiotoxicity, here Dox was formulated into a hyaluronan (HA) nanogel using ferric ion (Fe3+) coordination to control the intracellular distribution and release of Dox. Taking advantage of the paramagnetic properties of iron and the fluorescence of Dox, we conveniently monitored the targeted delivery of the HA@Dox nanogel in murine breast tumors through both T1-weighted magnetic resonance imaging and fluorescence imaging. Compared with free Dox, HA@Dox nanogel affords a CD44-targeted delivery, lysosomal distribution, pH-responsive release, and significant tumor inhibition. Mechanistically, the lysosome-enriched HA@Dox produces ROS, causing lysosomal membrane permeabilization, which further promotes the intracellular Dox distribution. This HA@Dox nanogel not only provides a facile cancer-targeted delivery but also successfully relieves Dox toxicity, representing a potent delivery system for Dox.
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CHEMICAL RESEARCH IN TOXICOLOGY
·
2024
·
IF 3.7
Ximei Li,Lihua Xin,Lan Yang,Yi Yang,Wei Li,Mingyu Zhang,Yufen Liao,Chen Sun,Weiwei Li,Ying Peng,Jiang Zheng
Amitriptyline (ATL), a tricyclic antidepressant, has been reported to cause various adverse effects, particularly hepatotoxicity. The mechanisms of ATL-induced hepatotoxicity remain unknown. The study was performed to identify the olefin epoxidation metabolite of ATL and determine the possible toxicity mechanism. Two glutathione (GSH) conjugates (M1 and M2) and two N-acetylcysteine (NAC) conjugates (M3 and M4) were detected in rat liver microsomal incubations supplemented with GSH and NAC, respectively. Moreover, M1/M2 and M3/M4 were respectively found in ATL-treated rat primary hepatocytes and in bile and urine of rats given ATL. Recombinant P450 enzyme incubations demonstrated that CYP3A4 was the primary enzyme involved in the olefin epoxidation of ATL. Treatment of hepatocytes with ATL resulted in significant cell death. Inhibition of CYP3A attenuated the susceptibility to the observed cytotoxicity of ATL. The metabolic activation of ATL most likely participates in the cytotoxicity of ATL.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Yuqi Yang,Shengjun Bu,Xiaoying Zhang,Qiqing Duan,Heyuan Meng,Zhuo Hao,Xiuxia He,Jiayu Wan
Infectious diseases caused by the influenza virus are the primary global concern. This study aimed to develop an electrochemical biosensor for detecting influenza A H1N1 virus based on duplex-specific nuclease (DSN) and rolling circle amplification (RCA) technologies. A single-stranded DNA (ssDNA) probe, comprising a recognition sequence for a target sequence of influenza virus H1N1-specific RNA sequence and primer sequence for initiating RCA, was designed and synthesized. A specific sequence of target H1N1 RNA triggered cyclical DSN cleavage of the probe. The released primer sequence then initiated RCA to generate detectable ssDNA-labeled multiple biotins. ssDNA was captured by streptavidin (SA) on the electrode surface and labeled with free Streptavidin-Horseradish Peroxidase (SA-HRP). The H1N1 influenza virus was quantitatively analyzed based on the changing current signal generated on the electrode surface. A linear range from 10 pM to 100 nM and a limit of detection of 0.44 pM were recorded. In addition, the biosensor demonstrated excellent selectivity, repeatability, and stability for analyzing H1N1 in real samples. This biosensor could be applied for the clinical diagnosis of influenza A H1N1 virus.
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RSC Advances
·
2012
·Vol. 2
·期 6
·2322-2328
·
IF 0
Yi Wang,Pu Zhang,Chun Fang Liu,Lei Zhan,Yuan Fang Li,Cheng Zhi Huang
An environmental friendly method for the preparation of functionalized reduced graphene oxide (rGO) in aqueous solution is developed with great easiness, using heparin as both a reducing agent and an effective stabilizer. Structural and morphological studies have demonstrated that part of the oxygen functionalities in the graphene oxide can be removed by this method, and heparin can be functionalized on the resulting rGO sheets through hydrophobic and hydrogen bonding interactions. The functionalized rGO shows good stability in aqueous solution, owing to the strong electrostatic and steric repulsions of the heparin which adsorb on the surfaces of rGO sheets. Moreover, the rGO exhibits excellent biocompatibility and anticoagulant activity, making it a promising candidate for widespread use in the biomedical field.
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CHROMATOGRAPHIA
·
2011
·Vol. 75
·期 1
·71-76
·
IF 1.075
Yang Xiujuan,Feng Jun,Chen Zuanguang,Liao Rifang,Li Xinchun
A novel, rapid and validated capillary electrophoretic method with an improved electromagnetic induction detector has been developed for the determination of ibuprofen, acetaminophen, amantadine hydrochloride, aminopyrine, diclofenac sodium and codeine phosphate in three kinds of analgesic pharmaceutical preparations. Fabrication of the electromagnetic inductor was the same as previously described, but a brand new electric circuit was designed for the detector to lower the background noise and improve the detection sensitivity. Important factors which might influence the response of the detector, including the value of the adjustable inductor and resistor, excitation frequency and voltage, were examined. Electrophoretic parameters affecting separation efficiency, such as variety of buffer, buffer pH, buffer concentration and electroosmotic flow modifier, were also deliberately investigated. Under the optimal conditions, highly linear response was obtained for these six compounds over three orders of magnitude with detection limits ranging from 0.1 to 1.0 μg mL −1 (S/N = 3). The average recovery and RSD were within the range of 97.5–101.5 and 1.1–2.3%, respectively. This simple, convenient, effective and stable method held great promise in quality control of pharmaceutical preparations.
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FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Ruimin Ran,Xiaoxia Zhang,Xin Guo,Changkai Yang,Fang Zhang,Guoying Li
Conventional Pickering emulsions can kill microorganisms by slow release of active substances to prevent foodborne contamination; however, their low bactericidal efficiency due to the absence of bacterial inducibility is a problem that needs to be addressed. Inspired by the fact that the growth of bacteria depends on the consumption of proteins and polysaccharides by microbial enzymes, the present study prepared a novel Pickering emulsion capable of intelligent sensing and targeted release using chitosan (CS)-soy protein isolate (SPI) colloidal particles as stabilizers and plant essential oils as active substances. The Pickering emulsion demonstrated optimal performance in achieving these goals when prepared with a CS:SPI ratio of 1:4, an oil fraction of 40%, at a homogeneous speed of 25 000 rpm, and utilizing cinnamon oil as the plant essential oil. This was evidenced by the emulsion having the smallest droplet size and the greatest stability, which can increase the chances of coming into contact with microorganisms and avoid premature demulsification. Antibacterial experiments demonstrated that the emulsion could control the bacteria-mediated release of essential oils, realizing intelligent sterilization. Experiments on banana preservation showed that the released essential oils effectively delayed banana spoilage. These findings contribute to the development of intelligent food preservation systems.
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ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Peiqian Zhou,Tuozhou Liu,Wenbin Liu,Lingshun Sun,Haifei Kang,Kun Liu,Peiyuan Luo,Youfa Wang,Ling Luo,Honglian Dai
The periosteum, rich in neurovascular networks, bone progenitor cells, and stem cells, is vital for bone repair. Current artificial periosteal materials face challenges in mechanical strength, bacterial infection, and promoting osteogenic differentiation and angiogenesis. To address these issues, we adjusted the electrospinning ratio of poly-ε-caprolactone and chitosan and incorporated Zn doping whitlockite with polydopamine coating into a nanofiber membrane. After a series of characterizations, optimal results were achieved with a poly-ε-caprolactone: chitosan ratio of 8:1 and 5% nanoparticle content. In vitro cell experiments and in vivo calvarial defect models, the sustained release of Mg2+ and Ca2+ promoted vascularization and new bone formation, respectively, while the release of Zn2+ was conducive to antibacterial and cooperated with Mg2+ to promote neurovascularization. Consequently, this antibacterial bionic periosteum with an angiogenesis-neurogenesis coupling effect demonstrates a promising potential for bone repair applications.
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Journal of Materials Chemistry A
·
2024
·Vol. 12
·期 44
·30351-30361
·
IF 10.7
Jiawei Huang,Weifeng Chen,Qiyan Kuang,Ting Xiao,Lihua Jiang,Xinyu Tan,Yizhu Lei
Passive radiative cooling technologies are crucial for energy conservation and emission reduction, yet their development encounters substantial challenges. In this study, we fabricate an advanced passive radiative cooling emitter by coating BaSO4@SiP/H-SiO2@PAN on an aluminum substrate. This design highlights a remarkable average temperature reduction of 20.1 °C and an ultrahigh cooling power of 121.0 W m−2 under strong solar radiation, showcasing exceptional sub-ambient cooling performance. Through meticulous design optimization, the emitter exhibits an efficient reflectance of 95.5% in the visible spectrum and a superior mid-infrared emissivity of 97.9%. Additionally, the emitter's superhydrophobic surface, characterized by a water contact angle (WCA) of 155°, ensures excellent self-cleaning properties. Furthermore, its performance remains stable even after prolonged UV exposure, demonstrating its outstanding durability. This innovative emitter provides a critical solution for energy-efficient cooling in buildings and outdoor equipment, significantly contributing to reduced energy consumption and carbon emissions.
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JOURNAL OF THE INDIAN CHEMICAL SOCIETY
·
2024
·
IF 3.2
Chengcheng Li,Hao Zheng,Xingguo Zhang,Zhihua Pu,Dachao Li
This paper reports an adaptive degradable flexible power supply. The power supply is produced using a batch-printed, easy-to-operate screen-printing technique that converts the chemical energy of glucose and oxygen into electricity. The power supply has stable current and voltage outputs in vitro, delivering a consistent open circuit potential of 0.2 V in vitro. Due to the flexibility and degradability of the power supply, the power supply can be implanted anywhere in the tissue. This breaks through the limitation of location, for example, mechanical energy harvesters can only be implanted in places with frequent movements. And due to the degradable nature of the power supply, surgical resection is avoided. Glucose and oxygen are ubiquitous and abundant in interstitial fluid. The in vivo experimental results prove that the power supply can power the light-emitting diode and have a stable current output. Therefore, the power supply is expected to provide internal power for implantable devices, avoiding transmission from external sources such as radio frequency.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Shiming Tang,Zhilin Ouyang,Ying Huo,Daocheng Liao,Zhiteng Zhang,Ying Lin,Suiping Zheng
Hydroxytyrosol has been proven beneficial to human health. However, the process involving the conversion of L-DOPA to 3,4-dihydroxyphenylacetaldehyde (3,4-DHPAA) in hydroxytyrosol biosynthesis typically required the simultaneous use of decarboxylase and oxidative deaminase. In addition, phenylacetaldehyde reductase from Solanum lycopersicum (SlPAR) in hydroxytyrosol biosynthesis exhibits poor thermal stability. In this study, we unexpectedly discovered that L-DOPA decarboxylase from Pseudomonas putida (PpDODC) exhibits weak dual-functional activity for both decarboxylation and oxidative deamination of L-DOPA. Through a dual-function reshaping strategy, the best dual-functional mutant PpDODC/Y79F/Y324F achieved an enzyme activity of 0.95 U/mg and exhibited a 256.8-fold increase in activity compared to the wild-type. Through rational design of SlPAR, the optimal mutant SlPAR/G52D/V188I/N234W/Q286P (SlPAR-M4) maintained stability after 12 h' treatment at 40 °C. Based on these mutants, we established a simplified cascade to synthesize hydroxytyrosol from L-DOPA, achieving a hydroxytyrosol yield of 31.4 mM from 32 mM L-DOPA in a 5-hour reaction. This process achieved the highest molar conversion rate (98.2 %) currently reported for the synthesis of hydroxytyrosol from L-DOPA. This study provides a novel solution for hydroxytyrosol synthesis from a new perspective, and the mutants hold promise for widespread application in biotransformation studies of hydroxytyrosol and other structurally-similar compounds.
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危险品化学品经营许可证（带存储）