DRUG DELIVERY
·
2018
·
IF 3.095
Shiwei Niu,David H. Bremner,Junzi Wu,Jianrong Wu,Haijun Wang,Heyu Li,Qianqian Qian,Hong Zheng,Limin Zhu
Nanoparticles and macromolecular carriers have been widely used to increase the efficacy of chemotherapeutics, largely through passive accumulation provided by their enhanced permeability and retention effect. However, the therapeutic efficacy of nanoscale anticancer drug delivery systems is severely truncated by their low tumor-targetability and inefficient drug release at the target site. Here, the design and development of novel l-peptide functionalized dual-responsive nanoparticles (l-CS-g-PNIPAM-PTX) for active targeting and effective treatment of GRP78-overexpressing human breast cancer in vitro and in vivo are reported. l-CS-g-PNIPAM-PTX NPs have a relative high drug loading (13.5%) and excellent encapsulation efficiency (74.3%) and an average diameter of 275 nm. The release of PTX is slow at pH 7.4 and 25 °C but greatly accelerated at pH 5.0 and 37 °C. MTT assays and confocal experiments showed that the l-CS-g-PNIPAM-PTX NPs possessed high targetability and antitumor activity toward GRP78 overexpressing MDA-MB-231 human breast cancer cells. As expected, l-CS-g-PNIPAM-PTX NPs could effectively treat mice bearing MDA-MB-231 human breast tumor xenografts with little side effects, resulting in complete inhibition of tumor growth and a high survival rate over an experimental period of 60 days. These results indicate that l-peptide-functionalized acid – and thermally activated – PTX prodrug NPs have a great potential for targeted chemotherapy in breast cancer.
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高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2018
·
IF 3.997
Chaoyang Li,Hua Zhang,Xianfeng Gong,Qiang Li,Xueling Zhao
The chemical stability, good biocompatibility and high drug loading capacity of zinc oxide nanoparticles (ZnO NPs) and their biomedical potentials make them a promising candidate for drug delivery. The aim of this study was to develop and assess a simple procedure for surface functionalization of ZnO NPs by N-acetyl- l -cysteine (NAC) for anticancer camptothecin (CPT) delivery. NAC capped ZnO NPs were successfully made using ZnCl 2 and NaOH in the presence of NAC. CPT was covalently conjugated to the surface of as-synthesized ZnO-NAC NPs. To characterize the synthesized conjugate product (ZnO-NAC-CPT NPs), X-ray diffraction, Fourier Transform Infrared spectroscopy, transmission electron microscopy, scanning electron microscopy, and dynamic light scattering method were used. Our results indicated that the ZnO-NAC-CPT NPs exhibit near-spherical morphology and uniform dispersion with an average diameter of ∼70 nm. The hemolysis assay showed that ZnO-NAC-CPT NPs has almost no hemolytic activity. In addition, MTT cytotoxicity assessment on A549 lung cancer cells revealed a drop of IC 50 values from 1.17 μg/mL (free CPT) to 0.66 μg/mL (ZnO-NAC-CPT NPs). This result showed an augmented cancer-inhibitory effect of nanoconjugate complex. In conclusion, the novel ZnO-NAC-CPT NPs could be considered for new therapeutic endeavors.
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高影响因子
AMINO ACIDS
·
2018
·Vol. 50
·期 11
·1549-1556
·
IF 2.906
Huang Lu,Li Yanxia,Lin Qi,Lou Benyong,Chen Yiting
l -Proline-modified gold nanochannel membrane ( l -Pro-GNM) was prepared and applied for the enantioselective permeations of amino acid enantiomers including tyrosine (Tyr), tryptophan (Trp) and phenylalanine (Phe). Experimental results show that l -Pro-GNM has enantioselectivities for Tyr and Phe enantiomers. Furthermore, the chiral recognition mechanism was studied by density functional theory (DFT) and reduced density gradient (RDG). DFT computational results illustrate that the fundamental chiral recognition system contains two chiral selectors and one selectand, which can be used to evaluate the enantioselective efficiencies of other chiral compounds and the enantioselective ability of other potential amino acid-modified GNM. Finally, graphs obtained by RDG using Multiwfn show helpful visual interactions between the chiral selector and selectand. Results indicate that the electrostatic interaction and hydrogen bonding are responsible for the binding of the chiral selector and selectand, and the larger binding energy shows larger van der Waals interactions.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2019
·
IF 3.714
Hafiz Muhammad Saleem Akhtar,Mohamed Abdin,Yahya Saud Hamed,Wei Wang,Guijie Chen,Dan Chen,Chunxu Chen,Wei Li,Shanza Mukhtar,Xiaoxiong Zeng
In the present study, polysaccharides (CHPS) were prepared from chickpea hulls by hot water extraction and ethanol precipitation, and the physicochemical, functional, thermal and structural characteristics of CHPS were investigated. The results revealed that CHPS was comprised of mannose , rhamnose, galacturonic acid, glucose, galactose , xylose and arabinose with molar percentages of 2.16, 2.96, 42.17, 9.87, 23.15, 6.29 and 13.38%, respectively. Functional analysis demonstrated that CHPS had good fat-binding, foaming and emulsifying properties. CHPS also exhibited good thermal stability. According to X-ray spectrum, CHPS was a semi-crystalline in nature which was also confirmed from scanning electron microscopic analysis. The solid-state spectrum of 13 C NMR reflected that CHPS were complex and heterogeneous polymers. Moreover, CHPS showed favorable inhibitory activity against α-amylase. These findings indicated that CHPS could be exploited as a hypoglycemic agent or multi-purpose additive in pharmaceuticals, cosmetics and food industries.
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TALANTA
·
2019
·
IF 4.916
Mohammad Chand Ali,Jia Chen,Haijuan Zhang,Zhan Li,Liang Zhao,Hongdeng Qiu
The efficient extraction of natural products from biomass is deemed an essential area of interest using green technology in the pharmaceutical and biochemical industries. In recent years, deep eutectic solvents (DESs) have been paying broad interest as compatible, safe, and inexpensive solvents. In this study, we addressed, for the first time, to give a fruitful illustration to extract bioactive compounds from favourite Lycium barbarum L. fruits using DESs as green designer solvents, and displaying the tuneability of DESs to extract bioactive compounds from biomass efficiently. Among the studied DESs, the 1 : 2 M mixture of choline chloride and p -toluene sulfonic acid (DESs-6) with an ultra-sound assisted extraction (UAE) method was more efficient to get high extraction yields of flavonoids including myricetin (57.2 mg/g), morin (12.7 mg/g), and rutin (9.1 mg/g) in net DESs. The resulting extraction method in which 50 mg of sample powder was extracted by UAE for 1.5 h using DESs-6 was found to be a more efficient process than conventional extraction methods such as heating plus stirring. Operational conditions including the extractant to sample solid ratio, the effect of water content, the effect of temperature, recycling of DESs, and the effect of ultra-sound irradiation time were investigated. The proposed DESs based extraction procedure attained recovery for flavonoids were in the range 75.6%–96.9%. Therefore, p -toluenesulfonic acid-based DESs may have strong potential as promising materials for the green and efficient extraction of pharmaceutical compounds from other plants or fruits.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2018
·
IF 3.862
Chenguang Yang,Wantong Song,Dawei Zhang,Haiyang Yu,Lei Yin,Na Shen,Mingxiao Deng,Zhaohui Tang,Jingkai Gu,Xuesi Chen
Gemcitabine is widely used for anticancer therapy. However, its short blood circulation time and poor stability greatly impair its application. To solve this problem, we prepared a poly ( l -glutamic acid)- g -methoxy poly (ethylene glycol)-gemcitabine conjugate ( l -Gem) with a 14.3 wt% drug-loading content. l -Gem showed concentration- and time-dependent cytotoxicity towards 4T1, LLC, MIA PaCa-2 and A2780 in vitro . Pharmacokinetic and biodistribution studies indicated that l -Gem had remarkably enhanced blood stability, prolonged blood circulation time and greatly improved selective tumor distribution compared with free gemcitabine. The area under the concentration–time curve from zero to infinity [AUC (0–∞) ] of l -Gem in plasma was 43-fold higher than that of free gemcitabine. The AUC (0–∞) of the inactive metabolite, 2′-deoxy-2′,2′-difluorouridine in the l -Gem group was ∼20% of that observed in the free gemcitabine group. The drug tumor accumulation ratio in the l -Gem group relative to the free gemcitabine group was 9.9 at 36 h, while the tumor AUC ratio was 15.8. Testing on Balb/C mice bearing the 4T1 tumor further demonstrated that l -Gem had significantly higher anticancer efficacy than free gemcitabine in vivo . These findings indicated that l -Gem has great potential for cancer treatment.
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ELECTROPHORESIS
·
2013
·Vol. 34
·期 6
·846-853
·
IF 3.261
Haizhi Zhang,Li Qi,Ying Shen,Juan Qiao,Lanqun Mao
Amino acid ionic liquids (AAILs) with l -lysine ( l -Lys) as anion were synthesized and applied as new chiral ligands in Zn(II) complexes for chiral ligand-exchange CE. After effective optimization, baseline enantioseparation of seven pairs of dansylated amino acids was achieved with a buffer of 100.0 mM boric acid, 5.0 mM ammonium acetate, 3.0 mM ZnSO 4 , and 6.0 mM [C 6 mim][ l -Lys] at pH 8.2. To validate the unique behavior of AAILs, a comparative study between the performance of Zn(II)- l -Lys and Zn(II)-[C 6 mim][ l -Lys] systems was conducted. In Zn(II)-[C 6 mim][ l -Lys] system, it has been found that the improved chiral resolution could be obtained and the migration times of the three test samples were markedly prolonged. Then the separation mechanism was further discussed. The role of [C 6 mim][ l -Lys] indicated clearly that the synthesized AAILs could be used as chiral ligands and would have potential utilization in separation science in future.
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JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL
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2012
·
IF 2.947
Hui-xue Ren,Han-jie Ying,Ping-kai Ouyang,Ping Xu,Jing Liu
Efficient and pollution-free catalysts play an essential role in the ultimate biodegradation of products in the synthesis of biodegradable and recyclable poly( l -lactic acid) (PLLA). Therefore, macroporous resin was utilized as the solid acid for the first time, on which lactate that could be absorbed in vivo was composited to catalyze the synthesis of PLLA. It was discovered that macroporous resin Amberlyst-15 showed good catalytic activity in oligomerization and polymerization, and lactate showed different catalytic efficiencies due to the differences in ionic radius and ionic charge. Compared to the traditional catalysts of SnCl 2 ·2H 2 O/TSA, the composite catalyst can improve the quality, yield and optical purity of its product, which can also be recycled without any residual in products. PLLA with molar mass of about 110,000 g mol −1 was synthesized at the yield of 93%. The product exhibits pure color (white), and its optical purity and crystallinity are 98% and 55%, respectively. A possible catalytic mechanism was proposed. The resulting PLLA products were characterized by GPC, 1 H NMR, DSC, and polarimeter.
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Analytical Methods
·
2014
·Vol. 7
·期 2
·684-689
·
IF 1.938
Yuan Su,Li Qi,Xiaoyu Mu,Minglin Wang
In this work, L-histidine-stabilized gold nanoclusters were prepared by a green one-pot process and employed as a selective probe for sensing ferric ions. The obtained probe exhibited bluish green fluorescence, and exhibited an excitation maximum at 394 nm and emission maximum at 492 nm. The fluorescence of the probe was obviously quenched by the introduction of ferric ions, hinting that the synthesized probe could be applied for sensing ferric ions. Further, the selectivity of the proposed probe for sensing ferric ions was investigated. The results indicated that the fluorescent probe had favorable sensitivity and selectivity for the detection of ferric ions. Moreover, this probe was further used for the analysis of ferric ions in bean sprouts. It showed that the gold nanocluster based fluorescent probe has great potential for sensing metal ions in plants.
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RSC Advances
·
2014
·Vol. 5
·期 3
·2039-2046
·
IF 3.708
Deli Xiao,Renfeng Pan,Siqiao Li,Jia He,Man Qi,Sumei Kong,Yu Gu,Rui Lin,Hua He
A bottom-up and wet-chemical method employing L-cysteine as a precursor has been proven to be an effective strategy for producing fluorescent porous CQDs for the first time, which exhibit stable and strong photoluminescence, and reach a quantum yield of 18.1%. Under optimum conditions, a good linear semilogarithmic correlation between the quenching efficiency (F0/F) and the concentration of chromium ions, even at 10−8 mol L−1, was achieved, and the limit of detection was 1.491 × 10−9 mol L−1. The quenching of the porous CQDs’ fluorescence intensity by Cr6+ may result from the inner filter effect. The application of these porous CQDs as probes for the detection of chromium ions is good, and they can be readily, rapidly and efficiently employed in the natural environment.
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INDUSTRIAL CROPS AND PRODUCTS
·
2014
·
IF 3.208
Yixin Tian,Zhan Li,Fei He,Yajing Guan,Shuijin Zhu,Jin Hu
Combining with seed coating method, rhodamine B (RB), a kind of fluorescent compounds had been used in seed labeling as an anti-counterfeiting technology. Effects of broad bean seed coating with RB on physiological parameters and fluorescence performance in broad bean seedlings were performed. The prolonged fluorescence in vascular bundles of seedlings treated with RB could be used as a marker of seed anti-counterfeiting. The experiment showed that RB had no negative effect on seed germination, seedling growth, seedling protective enzyme activities, and the contents of malondialdehyde (MDA) and chlorophyll, after coating seeds with ratio 1 kg RB to 10–30 kg seeds. In addition, the RB adsorption by soil was accorded with the isothermal adsorption equations of Langmuir, which exhibited the linear correlation as 0.9959, the maximum adsorption as 1.716 mg/g, and the adsorption equilibrium constant as 0.042 L/mg. The soil adsorption of RB could be effectively degraded by UV/Fenton. Present research suggested that coating broad bean seeds with appropriate concentration RB were safe to seed germination and this technology was suitable for anti-counterfeiting application.
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Analytical Methods
·
2015
·Vol. 7
·期 7
·3022-3027
·
IF 1.821
Bingdi Liu,Xin Zhang,Yaping Ding,Liqiang Luo,Fenfen Zhang
A new chiral biosensor fabricated by immobilizing bovine serum albumin (BSA) on gold-nanoparticle-modified glassy carbon electrodes, which could discriminate and detect phenylalanine (Phe) enantiomers, was proposed for the first time. The enantioselectivity was characterized by using scanning electron microscopy, cyclic voltammetry, electrochemical impedance spectroscopy and square wave voltammetry. The results showed that the BSA-modified electrode could enantioselectively recognize Phe enantiomers, and larger response signals were obtained from L-Phe. The factors influencing the performance of the biosensor were investigated. The enantiomeric composition of the L-/D-Phe mixture could be determined from the calibration curves. The modified electrodes have the advantages of simple operation, rapid detection and low cost.
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