BIOTECHNOLOGY LETTERS
·
2024
·
IF 2
Zhang Fei,Wang Xue,Pan Lixia,Wang Zhao,Zheng Jianyong
The α- l -rhamnosidase ( rha 1) gene was homologously expressed in Aspergillus niger strains CCTCC 206047 and CCTCC 206047Δ pyr G, using hygromycin B and auxotrophic as selection markers. The engineered A. niger strains RHA001-1 and RHA003-1 were screened, yielding α- l -rhamnosidase activities of 20.81 ± 0.56 U/mL and 15.35 ± 0.87 U/mL, respectively. The copy numbers of the rha 1 gene in strains RHA001-1 and RHA003-1 were found to be 18 and 14, respectively. Correlation analysis between copy number and enzyme activity in the A. niger strains revealed that α- l -rhamnosidase activity increased with the copy number of the rha 1 gene. Recombinant α- l -rhamnosidase was utilized for the enzymatic debittering of Ougan juice, and its process conditions were optimized. Furthermore, the primary bitter substance neohesperidin (2.22 g/L) in Ougan juice was converted into hesperetin 7-O-glucoside (1.47 g/L) and hesperidin (0.143 g/L). This study presents a novel approach for the production of food-grade α- l -rhamnosidase and establishes a technical foundation for its application in the beverage industry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Cheng-Hai Yan,Ding Wang,Ruo-Xi Mei,Lu Tan,Yi-Jiang-Cheng Li,Richard Ansah Herman,Yan Xu,Lu-Chan Gong,Jun Wang
Natural flavonoids exhibit diverse physiological effects and are used as functional factors, antioxidants and pigments in food as well as pharmaceutical and cosmetic applications. However, their poor solubility and stability, result in low bioavailability. Biomodification through deglycosylation effectively enhances their bioactivity. This study developed two surface display strategies in Escherichia coli , anchoring α -L-rhamnosidase RhaB1-ΔN to the cell surface using the anchoring protein Lpp-OmpA. Immunofluorescence and proteinase K sensitivity tests confirmed the successful construction of surface-displaying strains E. coli Lpp-OmpA-RhaB1-ΔN and E. coli RhaB1-ΔN-SpyCatcher-SpyTag. Enzymatic property studies revealed improved temperature and pH stability of the whole-cell catalysts with surface compared to free enzymes. After 6 catalytic cycles, E. coli Lpp-OmpA-RhaB1-ΔN retained higher activity than E. coli RhaB1-ΔN. In flavonoid hydrolysis, E. coli Lpp-OmpA-RhaB1-ΔN increased yields of isoquercitrin and kaempferol-3- O -glucuronide by 20.25% and 10.99%, respectively, compared with that in whole-cell catalyst with intracellular enzyme ( p < 0.05). In addition, it should be noted that although the enzyme expression of E. coli RhaB1-ΔN-SpyCatcher-SpyTag is reduced, its hydrolysis ability of flavonoids is comparable to that of E. coli RhaB1-ΔN due to the efficient catalytic ability of extracellular enzymes. This study establishes reliable whole-cell catalysts with surface-displayed α -L-rhamnosidase for efficient natural flavonoid modification, with potential applications in the food and pharmaceutical industries.
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
He Wan,Xiuqiang Chen,Kuiyuan Cao,Yongwei Han,Xixin Duan,Zhong Sun,Junyou Shi
Formic acid (FA) plays a key role in various chemical processes and industrial procedures. Attractively, FA can be produced from renewable biomass instead of fossil resources. In this paper, FA was successfully prepared with the help of isopolyacid Na 6 [ α -Mo 6 V 2 O 26 ] (Mo 6 V 2 ), which is superior to Keggin-type heteropoly- acids containing vanadium because its stronger oxidizing ability endows Mo 6 V 2 with better ability to break C-C bonds. Some carbohydrates and biomass, including glucose, fructose, xylose, hemicellulose, cellulose, corn stover, and birch, acted as sources to prepare FA in aqueous solution in the presence of Mo 6 V 2 . For monosaccharides, Mo 6 V 2 can accelerate the reaction rate and obtain the highest FA yield of 62 % from xylose. For hemicellulose, the yield of FA reaches up to 54 % in only 8 min. In the case of insoluble biomass materials, the combination of p -toluenesulfonic acid (TsOH) and Mo 6 V 2 can boost the catalytic performance of the system, with the FA yield being 47 % from cellulose, 37 % from corn stover, and 41 % from birch. Therefore, Mo 6 V 2 can be valued as an universital and promising catalyst for transforming renewable waste biomass to high-value chemicals.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD SCIENCE
·
2024
·
IF 3.2
Fengjiao Fan,Chenlinrui Yue,Zhenni Zhai,Hailu Liao,Xiaoni Lian,Hongkai Xie
Scallops are rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid but perishable due to their microbial growth and lipid oxidation. In this study, gelatin/dextran films containing cinnamaldehyde and α-tocopherol (0% + 0%, 0.3% + 0.3%, 0.6% + 0.6%, 0.9% + 0.9%, and 1.2% + 1.2%, w/w) as active fillers were developed by solution casting method, and their preservation effects on scallop adductor muscle refrigerated at 4°C for 0, 3, 6, 9, and 12 days were evaluated. Inclusion of the two active fillers did not influence the thermal stability of the films but created heterogenous and discontinuous film microstructure and increased the film hydrophobicity. Increase in the concentrations of active fillers lowered the mechanical properties and water vapor permeability of the films but increased their crystallinity, thickness, water contact angle, opacity, antibacterial property, and antioxidant property. The longest release times for both cinnamaldehyde and α-tocopherol were found in 95% (v/v) ethanol solution. The gelatin/dextran films containing 1.2% (w/w) of active fillers (Gelatin [Ge]/Dextran [Dx]/1.2 film) improved the chemical stability of refrigerated scallop adductor muscle. The total viable count (TVC) of the unpackaged scallop adductor muscle exceeded the recommended limit of 7 lg CFU/g on day 6 (7.07 ± 0.50 lg CFU/g), whereas the TVC of the Ge/Dx/1.2 film-packaged scallop adductor muscle was still below the limit on day 9 (5.60 ± 0.50 lg CFU/g). Thus, the Ge/Dx/1.2 film can extend the shelf life of refrigerated scallop adductor muscle by at least 3 days. Overall, the developed gelatin/dextran active packaging films are promising for the preservation of aquatic food products.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
·
2024
·
IF 2.7
Yuqing Zhang,Yaping Li,Yuhan Zhai,Xing Zhao,Mingxing Lv,Shaoxuan Yu,Haifang Xiao,Yuanda Song
Inhibition of α-glucosidase activity is a promising method to prevent postprandial hyperglycemia. The inhibitory effect and interaction of chrysin and diosmetin on α-glucosidase were studied in this study. The results of inhibition kinetics showed that chrysin and diosmetin reversibly inhibited α-glucosidase activity with IC50 value of 26.445 ± 1.406 μmol L−1 and 18.380 ± 1.264 μmol L−1, respectively. Further research revealed that chrysin exhibited a mixed-type inhibitory pattern against α-glucosidase, while diosmetin was noncompetitive inhibitory with Ki value of (2.6 ± 0.04) ×10−4 mol L−1. Fluorescence spectroscopy showed that both chrysin and diosmetin could quench the intrinsic fluorescence of α-glucosidase, the maximum emission wavelength of tyrosine (Tyr) and tryptophan (Trp) were not moved by chrysin, but red shifted by diosmetin. UV-Vis, fourier transform infrared spectroscopy (FT-IR) and circular dichroism (CD) measurements showed that the secondary structure and microenvironment of α-glucosidase were changed by chrysin and diosmetin. Further analysis of molecular docking showed that chrysin and diosmetin could bind with α-glucosidase and might cause the decrease of α-glucosidase activity. The results of molecular dynamics (MD) simulation showed that the stability of chrysin (or diosmetin)-α-glucosidase complex system was changed during binding process. In conclusion, chrysin and diosmetin are good α-glucosidase inhibitors.Communicated by Ramaswamy H. Sarma
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF POWER SOURCES
·
2025
·
IF 7.9
Xiaodong Liang,Xin Liu,Peixiong Wang,Zhongxian Guo,Xin Chen,Jia Yao,Jingying Li,Yi Gan,Lin Lv,Li Tao,Hanbin Wang,Houzhao Wan,Jun Zhang,Hao Wang
Manganese-based cathode materials for zinc-ion batteries provide a moderate voltage window and good specific capacity. However, the disproportionation reactions cause manganese to dissolve during the charge and discharge process, seriously affecting its cycle stability. Herein, nickel-doped α phase manganese dioxide (Ni-MnO 2 ) obtained by ion exchange effectively improves the lattice stability and cycle stability of manganese dioxide (MnO 2 ). The introduction of Ni 2+ supports the lattice structure of MnO 2 , reduces structural damage and manganese (Mn) dissolution caused by the Jahn-Teller effect during the cycle, and improves its electronic conduction and ion migration capabilities. It quickly completes the activation process at a current density of 0.1 A g −1 and stably maintains a capacity of 233 mAh g −1 . After 2800 cycles at 1 A g −1 , the capacity retention rate remains 96.3 %. In contrast, MnO 2 without ion exchange modification has dropped to 19.6 % after 130 cycles. This paper demonstrates the feasibility of the ion exchange process, bringing more precise and effective ideas to the research and development of high-performance aqueous zinc-manganese batteries.
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Rui Wang,Chenyu Shang,Qiqi Zhao,Qi Huang,Chenyang Liu,Wenli Xu,Rui Liu,Weiwei Zhang,Jianxu Ding
Cubic α-CsPbI3 is recognized as one of the most promising semiconductors for optoelectronic applications owing to its suitable band gap, excellent optoelectronic performance, and thermal stability. However, its phase instability from α to δ poses a significant obstacle for practical applications. How to stabilize α-CsPbI3 becomes a paramount issue. Here, we propose a strategy in which α-CsPbI3 nanocrystals (NCs) are in situ generated in the Cs4PbI6 single-crystal (SC) matrix through I ion migration, facilitated by its ultralow activation energy (0.104 eV). The existence of the I vacancy can promote ion migration and can increase the content of α-CsPbI3. As α-CsPbI3 NCs are generated in situ, the light yellow Cs4PbI6 SCs convert to black. The lattice of Cs4PbI6 twines outside of α-CsPbI3 NCs without a grain boundary and restricts the migration of I and Cs within the α-CsPbI3 lattice. This mechanism effectively stabilizes α-CsPbI3 for over 2 years without experiencing a phase transition. Such stable α-CsPbI3 NCs exhibit deep red emission (700 nm), a high exciton binding energy (55.5 meV), and a long carrier lifetime (176.9 ns). Furthermore, annealing at 320 °C for 12 h did not result in any damage. Additionally, white-light-emitting diodes (WLEDs) were fabricated using black Cs4PbI6 SCs and yellow phosphor YAG:Ce3+ under an atmospheric environment and realized white emission. The WLEDs maintain approximately 86% luminescence after 18 days or under continuous operation for 12 h, demonstrating high stability and eliminating the need for short-wavelength blue chips. Overall, the α-CsPbI3 NCs deviating from Cs4PbI6 SCs offer an approach to realize highly efficient light-emitting diode applications.
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Lichun Liu,Ziye Zhang,Zihao Li,Hao Wang,Zhenxing Li,Hong Lin
The incidence of shrimp-induced food allergy is increasing yearly. Glycation has been reported to reduce shrimp tropomyosin (TM) allergenicity, and how advanced glycation end products (AGEs) impact TM allergenicity has garnered worldwide attention. This work investigated the conformational and epitope alterations of TM under α-dicarbonyl compounds (glyoxal (GO), methylglyoxal (MGO), butanedione (BU)) glycation and conformation-allergenicity relationship. As the results, α-dicarbonyl compounds glycation induced high glycation and TM conformational alterations. AGEs glycation sites on TM epitopes were identified by HPLC-MS/MS, with epitope destruction rates of 62.5 %, 50 % and 62.5 % for GO-TM, MGO-TM and BU-TM, respectively. Immunological results revealed TM glycated by α-dicarbonyls compounds performed weaker IgE binding by 33.38–33.86 %, while GO and MGO reduced IgG binding by 63.60 % and 77.72 %, respectively, suggesting α-dicarbonyls compounds reduced TM allergenicity via AGEs-induced epitope loss and conformational changes. This investigation could provide novel insights into how α-dicarbonyls compounds reduce TM allergenicity and prevent shrimp-induced allergy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Joule
·
2024
·
IF 38.6
Chunjie Huang,Shan Tan,Bingcheng Yu,Yiming Li,Jiangjian Shi,Huijue Wu,Yanhong Luo,Dongmei Li,Qingbo Meng
Meniscus coating technique is extensively employed for fabricating large-area perovskite films. Based on this technique, there are still challenges of formamidinium lead triiodide (FAPbI 3 ) nucleation and crystallization in the film-forming process, which significantly hinders the device performance of perovskite solar cell (PSC) modules. Here, we developed a kind of meniscus-modulated blade coating method combined with solvent engineering to realize scalable, high-quality α-phase FAPbI 3 films with larger grain sizes, preferred crystal orientation, excellent uniformity, and controllable thickness. On this basis, a notable 25.31% power conversion efficiency (PCE) for small-area cells (0.09 cm 2 ) and 23.34% PCE for minimodules (aperture area: 12.4 cm 2 ) with a certified PCE of 23.09% have been achieved. Besides, this minimodule exhibited exceptional device stabilities by remaining above 93% of the initial value after 2,000 h outdoor aging testing. This work provides a very promising meniscus coating fabrication method to realize high-performance FAPbI 3 perovskite solar cells and photovoltaic modules.
✓ 已验证
产品关联
DOI
高影响因子
Cell Reports
·
2024
·
IF 7.5
Chenyin Wang,Meigui Yang,Dongyao Liu,Chaogu Zheng
Summary Microbial metabolites that can modulate neurodegeneration are promising therapeutic targets. Here, we found that the short-chain fatty acid propionate protects against α-synuclein-induced neuronal death and locomotion defects in a Caenorhabditis elegans model of Parkinson’s disease (PD) through bidirectional regulation between the intestine and neurons. Both depletion of dietary vitamin B 12 , which induces propionate breakdown, and propionate supplementation suppress neurodegeneration and reverse PD-associated transcriptomic aberrations. Neuronal α-synuclein aggregation induces intestinal mitochondrial unfolded protein response (mitoUPR), which leads to reduced propionate levels that trigger transcriptional reprogramming in the intestine and cause defects in energy production. Weakened intestinal metabolism exacerbates neurodegeneration through interorgan signaling. Genetically enhancing propionate production or overexpressing metabolic regulators downstream of propionate in the intestine rescues neurodegeneration, which then relieves mitoUPR. Importantly, propionate supplementation suppresses neurodegeneration without reducing α-synuclein aggregation, demonstrating metabolic rescue of neuronal proteotoxicity downstream of protein aggregates. Our study highlights the involvement of small metabolites in the gut-brain interaction in neurodegenerative diseases.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yingxin Liu,Fangliang Li,Tao Fei,Xue Lin,Lu Wang,Zhonghua Liu
Aquilaria sinensis leaves have long been consumed as a popular replacement tea for lowering postprandial blood glucose levels, but their specific functional components remain unclear. In this study, Aquilaria sinensis leaf-tea 70 % ethanol extract (ALTE) exhibited excellent anti-α-glucosidase activity (IC 50 = 6.93 ± 1.91 μg/mL) and promoted glucose consumption ability in 3 T3-L1 preadipocyte cells. Subsequently phenolic compositions of ALTE were identified for the first time. After that, five potential α -glucosidase inhibitors (α-GIs) including cynaroside-3,5-diglucose, malvidin 3-glucose, epicatechin, epigallocatechin gallate, and dihydromyricetin in ALTE were screened using a targeted bio-affinity ultrafiltration-HPLC/MS method. Moreover, these five α -GIs all showed good anti-α-glucosidase effects and glucose consumption-promoting ability. Furthermore, the binding properties and inhibition mechanisms of five α-GIs to α-glucosidase were further analyzed via enzyme inhibition kinetics, molecular docking, and molecular dynamics simulation. This study confirms that Aquilaria sinensis leaf-tea is effective in preventing post-hyperglycemia in vitro models, suggesting potential for future research in human trials.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Guihong Yu,Xiangji Fu,Xuhua Mo,Lingling Tan,Song Yang
Bioactivity screening revealed that the EtOAc extract from the culture broth of Phellinus igniarius SY489 exhibited remarkable α -glucosidase inhibitory activity, with an IC 50 value of 1.92 μg/mL. Activity- and ultraviolet (UV) profile-guided isolation led to the discovery of four anti-diabetic styrylpyrones ( 1 – 4 ), including two novel compounds, phelignidins A ( 1 ) and B ( 2 ). Compounds 1 and 2 represent a rare structural type of styrylpyrone dimer, in which one of the pyrone moieties exists in an open-ring state. The absolute configurations of the new compounds 1 and 2 , as well as the previously unresolved compound 3 , were established. Compounds 1 – 4 were effective in α -glucosidase inhibition, anti-glycation, and antioxidant assays, surpassing or being comparable to the positive control drugs, with minimal cytotoxicity. Furthermore, studies on α -glucosidase inhibition mechanisms suggested that these compounds interact with α -glucosidase at a single binding site, causing secondary structure unfolding and exerting inhibitory activity via a mixed-type mechanism. These results provide an important basis for developing novel, low-toxicity, multi-target anti-diabetic drugs from edible and medicinal fungi.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）