Biosensors-Basel
·
2025
·Vol. 15
·期 10
·700
·
IF 5.6
Yu Qin,Jiali Xie,Shujun Zhen
Fluorescence anisotropy (FA) has been widely used for analyzing biomolecules due to its high throughput, homogeneous detection, and strong resistance to photobleaching. However, the traditional FA method suffers from low sensitivity when the target molecules are small and rotate rapidly, often producing insignificant changes in the FA value. In this study, by combining double signal amplification through the trans-cleavage of CRISPR-Cas12a and DNA tetrahedron assemblies with a large molecular size, a new, fast, simple and highly sensitive FA method was constructed to achieve the quantitative detection of hepatitis B virus DNA (HBV-DNA). The experimental results showed that the linear range of this method was 0.5–9 nmol/L, and the detection limit (LOD = 3σ/k) was 48 pmol/L. In addition, the method demonstrated excellent selectivity and anti-interference, and it was successfully applied to detect HBV-DNA in human serum, indicating that this method has the potential for clinical diagnosis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DIAMOND AND RELATED MATERIALS
·
2026
·
IF 5.2
Jiaqi Yu,Shiwen Wang,Minghong Xie,Qiong Wu,Qing Shu
To overcome rapid charge recombination, a direct Z -scheme Zn 3 V 2 O 8 /g-C 3 N 4 heterojunction was designed, thus boosting photocatalytic performance. A suite of composite photocatalysts, with meticulously tuned Zn 3 V 2 O 8 mass ratios (x = 40, 60, and 80 wt%), were synthesized via a facile hydrothermal route. Comprehensive characterization, including XRD, SEM, UV–Vis DRS, PL spectroscopy, and photo-electro-chemical measurements confirmed the formation of intimate interfacial contact between Zn 3 V 2 O 8 and g-C 3 N 4 . This tailored interfacial architecture effectively promotes visible-light absorption and facilitates the separation and transfer of photo-generated charge carriers. Notably, the Zn 3 V 2 O 8 (60 wt%)/g-C 3 N 4 composite achieved 91% degradation of RhB within 120 min under simulated solar irradiation, substantially outperforming pristine g-C 3 N 4 (57%). Radical trapping experiments and electron spin resonance (ESR) spectroscopy identified superoxide radicals ( O 2 − ) and holes (h + ) as the primary active species. Based on these findings, a direct Z -scheme charge transfer mechanism is proposed, wherein photoinduced electrons in the conduction band of Zn 3 V 2 O 8 recombine with holes in the valence band of g-C 3 N 4 . This synergistic recombination pathway preserves the most energetic electrons in g-C 3 N 4 and holes in Zn 3 V 2 O 8 , endowing the system with strong redox capacity for the simultaneous generation of O 2 − and OH radicals, which collaboratively drive the efficient degradation of RhB. This study provides a rational design framework for constructing high-performance g-C 3 N 4 -based Z -scheme photocatalytic systems.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MEDICINAL CHEMISTRY
·
2026
·
IF 7.3
Xinyue Zang,Peigen Wei,Chunxia Xu,Jiuyang Sun,Xiuling Yu,Deyin Wang,Zaixiang Zhang,Chang Liu,Feng Feng,Ruoping Wang,Yang Yang
The design of multimechanistic conjugates based on 10-hydroxycamptothecin (10-HCPT) offers a promising approach to reduce toxicity and enhance antitumor efficacy. We designed and synthesized a novel series of conjugates by linking melampomagnolide B (MMB) to 10-HCPT/SN-38 via diverse linkers. Among them, 6i1/6i2 exhibited remarkable anticolorectal cancer potency and minimal cytotoxicity toward normal THLE-2 cells. Mechanistic studies indicated that 6i1/6i2 could inhibit IκB kinase β (IKKβ), leading to the inhibition of p65 and IκBα phosphorylation, suppression of p65 nuclear translocation, and subsequent regulation of genes controlled by nuclear factor kappa-B (NF-κB). Additionally, they targeted topoisomerase I (Topo I), inducing DNA damage, reactive oxygen species (ROS) accumulation, mitochondrial membrane potential loss, and S-phase arrest. In a CT-26 xenograft model, 6i1 and 6i2 (5 mg/kg) potently suppressed tumor growth, achieving TGI rates of 89.9% and 95.4% without observable toxicity.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2022
·
IF 6.371
Zuocheng Luo,Qingfeng Zhang,Wei Xie,Tong Yuan,Qisheng Zang,Ning Wang,Hanxiao Fan,Shuhong Xie,Xiaoping Ouyang
Potassium ion hybrid capacitors (PIHCs) have attracted widespread attention as combining the advantages of potassium ion batteries (PIBs) with supercapacitors . However, the sluggish kinetics caused by the large radius of K + hinder the practical application of PIHCs. Herein, we synthesized a boron and fluorine co-doping interlaced network porous carbon nanofibers (BF-PCNFs), which was used as the freestanding anode of PIHCs the freestanding anode of PIHCs to alleviate large volume expansion and increase conductivity. Benefiting from the B, F co-doping and porous interlace nanostructure of BF-PCNFs, the anode shows 185 mA h g −1 under a load of 12.6 mg cm −2 , a reversible capacity of 176 mA h g −1 at 1000 mA g −1 after 6000 cycles, and the capacity retention from 100 mA g −1 to 5000 mA g −1 is 62%. Furthermore, the PIHCs based on BF-PCNFs as anode and activated carbon as cathode exhibit an energy density of 87 Wh kg −1 and a power density of 393 W kg −1 . The capacity retention is as high as 78% over 4000 cycles. Considering the exceptional rate performance, long cyclability and high-mass-loading, our work may supply a new strategy for designing novel energy storage devices with outstanding performance.
研究领域:
材料科学
·
电化学
·
能源存储
·
纳米科技
·
功能材料
✓ 已验证
DOI
高影响因子
CRYSTAL GROWTH & DESIGN
·
2024
·
IF 3.2
Juhe Liu,Yuwei Chen,Mengran Sun,Wenhao Liu,Xianghe Meng,Jiyong Yao
Na3LuB8O15, the first sodium lutetium borate in the Na–Lu–B–O system, was prepared by the spontaneous crystallization method. Na3LuB8O15 is crystallized in the centrosymmetric triclinic space group P1¯ with the following cell parameters: a = 6.4047(12)Å, b = 7.3342(13)Å, c = 13.081(2)Å, α = 88.999(7)°, β = 78.631(8)°, γ = 66.622(7)°, and Z = 2. The general configuration of Na3LuB8O15 can be characterized as a network structure created by infrequent multiboron anion chain skeletons [B8O17] and [LuO7] polyhedra shared by oxygen atoms, where Na ions are filled in the pores to balance charges. According to a UV–vis-NIR spectrum investigation, the chemical has a short ultraviolet cutoff edge (185 nm). Thermal analysis reveals incongruent melting compound properties. In addition, a first-principles calculation was conducted to delve deeper into the correlation between the performance and the structure.
研究领域:
材料科学
·
无机化学
·
晶体学
·
光学材料
·
固态化学
✓ 已验证
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8
Yongxing Li,Haiyu Ji,Shuang Yang,Wenhao Cao,Jiarong Zou,Zhangliang Zhu,Jinfang Zhang,Mei Li
Interactions between polyphenols and starch are critical for modulating starch functionality through structural reorganization. This study systematically compared the interaction of young apple polyphenols (YAP) with A-type (corn starch, CS), B-type (potato starch, POS), and C-type (pea starch, PES) under autoclaving. Multi-technique analysis confirmed the formation of starch-YAP complexes and structural reorganization into A+V- and B+V-type crystalline polymorphs. Distinct differences in complex properties were observed among the crystal types. CS-based complexes (CSY_4) demonstrated the highest loading capacity (785.61 ± 0.37 mg/g) and long-range crystallinity (9.35%), yet exhibited the lowest short-range order (0.885 ± 0.044); PES-based complexes (PESY_4) showed the highest encapsulation efficiency (97.40 ± 0.02%) alongside the lowest swelling power (1.63 ± 0.01 g/g) and solubility (0.36 ± 0.01 g/g); whereas POS-based complexes (POSY_4) presented the lowest amylose content (13.71 ± 0.17%). These structural modifications led to markedly suppressed digestibility, with resistant starch content increasing to 52.02 ± 2.41% (CSY_4), 56.55 ± 0.43% (POSY_4), and 57.19 ± 1.80% (PESY_4), and POS demonstrated the greatest efficacy in lowering the estimated glycemic index (eGI) (ΔeGI = 14.83). The study elucidates a crystal-type-dependent strategy for designing slow-digesting starch-based functional foods through polyphenol-mediated structural engineering.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2026
·
IF 5.7
Meilin Qiao,Licheng Gao,Ruibing Yu,Jian-Wei Chen,Xuanrong Sun,Yue Cai
The emergence of multidrug-resistant Pseudomonas aeruginosa (P. aeruginosa) infections, particularly those involving biofilm formation, poses a critical challenge to global healthcare. While polymyxin B (PMB) remains a last-line antibiotic against such infections, its efficacy is severely limited by poor biofilm penetration and adaptive resistance mechanisms. In this study, we developed a biomimetic nanoplatform (PMφ-PLGA-NOR) comprising a poly(lactic-co-glycolic acid) (PLGA) core loaded with norharmane (NOR), coated with PMB-modified P. aeruginosa cytoplasmic membrane (PMφ). This design utilizes the homologous targeting of bacterial membrane components and PMB's affinity for gram-negative pathogens to achieve efficient biofilm penetration. NOR, a βcarboline compound, has been shown to disrupt quorum sensing (QS) by inhibiting PqsA, resulting in significant reduction in biofilm formation. In vitro investigations have demonstrated that PMφ-PLGA-NOR disrupts the integrity of biofilms, thereby enhancing the bactericidal efficacy of PMB by facilitating its deep penetration into the biofilm matrix. In vivo, intraperitoneal administration of Mφ-PLGA-NOR combined with PMB achieved a 2-log reduction in bacterial lung burden. This study underscores the promise of bacterial plasma membrane-based pathogen-mimetic nanoplatforms in potentiating the efficacy of antibiotic adjuvants in conjunction with biofilmpenetrating strategies.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ChemCatChem
·
2026
·Vol. 18
·期 14
·e70948
·
IF 4.1
Biao Jiang,Chunyang Zhang,Zechang Chen,Rui Song,Maochang Liu
Protonic ceramic electrolysis cells (PCECs) offer a low‑temperature route for efficient hydrogen production, but their performance is often limited by poor catalytic activity and insufficient stability of air electrodes. Herein, we report a synergistic strategy combining A‑site deficiency with B‑site high‑entropy design to simultaneously enhance triple ionic-electronic conductivity (e − /O 2− /H +) and structural stability. The resulting perovskite, Ba 0.8 Co 0.2 Fe 0.2 Zr 0.2 Y 0.2 Ni 0.2 O 3−δ (B 0.8 CFZYN), exhibits abundant oxygen vacancies, favorable hydration thermodynamics, and expanded electrochemically active sites from the triple‑phase boundary to the entire electrode bulk. The electrode achieves an exceptionally low polarization resistance of 0.146 Ω cm 2 at 650°C in humid air. In a single PCEC, current densities reach 1278.9, 873.5, and 531.7 mA cm −2 at 1.3 V and 650°C, 600°C, and 550°C, respectively. Moreover, the cell shows negligible degradation over 250 h at 600°C. This work demonstrates that high‑entropy defect engineering provides a powerful platform for designing highly active and robust electrocatalysts for intermediate-temperature PCECs. Graphical Synergistic A‑site deficiency and B‑site high‑entropy design in Ba 0.8 Co 0.2 Fe 0.2 Zr 0.2 Y 0.2 Ni 0.2 O 3-δ enhances oxygen vacancies and hydration kinetics, enabling superior triple conduction. The PCEC delivers 873.5 mA cm −2 at 1.3 V and 600°C, with excellent durability over 250 h of steam electrolysis.
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Jiawei Zheng,Xuan Hu,Jiufang Chen,Fengxia Lan,Li Xu,Zeping Lu,Binbin Nian,Nanjing Zhong
CALB@GA-NH 2 -UiO-66 exhibited 97.31% oleic acid conversion and 77.60% DAG content. • CALB@GA-NH 2 -UiO-66 retained full initial activity after 11 reuse cycles. • The immobilized CALB exhibited a single conformational basin. • Covalent enzyme–carrier conjugates were formed via Schiff base linkages. • The immobilization moderately restrained the flexibility of α-helix 5 of CALB. This study aimed to develop a robust biocatalyst for diacylglycerol (DAG) synthesis. Candida antarctica lipase B (CALB) was immobilized on NH₂-UiO-66 via glutaraldehyde (GA) cross-linking. The cross-linked CALB exhibited significantly better performance than the adsorbed counterpart. Oleic acid conversion reached 97.31% with DAG content up to 77.60%. Moreover, the immobilized CALB retained its full initial activity after 11 reuse cycles. The temperature dependence of oleic acid conversion followed the equation ln V₀ = 8.86–4.67 × 10 3 / T , with an activation energy of 38.83 kJ·Mol −1 . Molecular dynamics simulations revealed that the flexibility of α-helix 5 in CALB@GA-NH₂-UiO-66 is moderately restrained, and the immobilized CALB exhibits a single conformational basin. Additionally, immobilization induces slight contraction of the CALB active site pocket, and reduced length and increased bottleneck radius of the tunnel. This study provides molecular insights into the structure–stability relationship of immobilized enzymes, guiding the rational design of robust biocomposites We develop a robust biocatalyst via glutaraldehyde cross-linking of CALB onto NH₂-UiO-66 for DAG production by esterification. Download: Download high-res image (210KB) Download: Download full-size image
✓ 已验证
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2026
·
IF 6
Lan Peng,Jian Chen,Chang-cong Huang,Sheng-jun Liao,Chen-xi Gao,Jia-shen Chen,Hui Zhang,Zheng-ren Huang
SiC has attracted extensive interest for semiconductor electronic devices, due to its wide bandgap and favorable physical properties. SiC ceramics with high resistivity and high thermal conductivity were fabricated by spark plasma sintering with B doping (0.5–3.0 wt.%) and Y 2 O 3 addition. B doping promoted grain growth from 0.57 to 1.48 μm and increased resistivity from 10 5 to 10 9 Ω·cm. The resistivity enhancement is attributed to the B–N compensation mechanism. B doping also enhances resistivity stability under high electric fields and increases the threshold field for the nonlinear-to-linear conduction transition from 2 to 20 V·mm -1 . The sample with 2.5 wt.% B exhibits a room-temperature resistivity of 1.1 × 10 9 Ω·cm, which remains higher than 10 4 Ω·cm at 873 K. The SiC ceramics retain high thermal conductivity (97.1–139.5 W·m -1 ·K -1 ) and exhibit a slower decrease to lower than 600 K compared with undoped SiC. These results support the use of SiC ceramics in semiconductor applications, such as integrated circuit packaging substrates and electrostatic chucks (ESCs)
✓ 已验证
DOI
高影响因子
Nature Communications
·
2026
·Vol. 17
·期 1
·3670
·
IF 18.1
He Di,Zuo Wenting,Xiang Zhiguang,Zhao Jiankang,Tong Wei,Li Hongyan,Fang Qing,Li Xin,Zhang Yun,Zheng Ying,Zhuo Xianxia,Pu Danni,Huang Yijiao,Yuan Yingying,Wang Weiyang,Lu Yameng,Luo Min,Wang Peigang,Wang Zai,Cao Bin
Respiratory viral infections, such as influenza and COVID-19, pose significant global health challenges. For patients with invasive pulmonary aspergillosis, a subsequent viral infection can lead to markedly worse clinical outcomes. Although amphotericin B (AmB) remains a cornerstone antifungal therapy, our investigation demonstrates that it paradoxically enhances the entry of influenza A virus and SARS-CoV-2. Mechanistically, AmB directly binds to and activates glucocerebrosidase, leading to ceramide accumulation and RAB7 upregulation in the late endosomes, thereby enhancing late endosomal maturation and fusion with viruses. In animal models, AmB treatment enhances viral infection in both influenza A virus–infected mice and SARS-CoV-2–challenged hamsters, resulting in accelerated weight loss, higher viral loads, and aggravated tissue damage. Consistently, in our propensity score-matched cohort of patients with culture-confirmed invasive pulmonary aspergillosis (2016–2025, n = 1,072), systemic use of AmB is associated with a significantly higher incidence of subsequent viral infection compared to other antifungals (21.55% vs. 7.76%, P = 0.003), which is further supported by multivariable analysis confirming AmB as an independent risk factor (adjusted OR = 3.45, 95% CI 2.20–5.41, P = 7.174 × 10 -8 ). In summary, our findings provide crucial clinical evidence to guide antifungal therapy and reveal glucocerebrosidase as a potential target for developing novel antiviral strategies.
✓ 已验证
DOI
高影响因子
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
·
2026
·
IF 5.3
Can Chen,Xiaofei Chen,Xilian Huang,Yaping Xie,Pengfei Shi,Shenxian Qian,Zhenzhen Chen
Shikonin, a natural compound, exhibits antitumor effects in DLBCL, but its mechanism remains unclear. Shikonin’s cytotoxicity in DLBCL cells was assessed by MTT assays. Ferroptosis and lipid peroxidation markers were analysed via flow cytometry and biochemical kits. Mechanisms were explored using gene knockdown/overexpression, dual-luciferase assays, RNA pull-down, proteomics, RIP, FISH, and ChIP. Key proteins and genes involved in ferritinophagy and ferroptosis were examined by Western blot, RT-qPCR, and immunofluorescence, with findings validated in a nude mouse xenograft model. Shikonin triggers ferritinophagy and ferroptosis by increasing iron accumulation and lipid peroxidation. LncRNA ADPGK-AS1 protects against ferroptosis by regulating MT2A via eIF4G1 interaction. Shikonin triggers ferritinophagy and ferroptosis by disrupting iron metabolism and redox balance. The eIF4G1–ADPGK-AS1/MT2A axis mitigates these effects, revealing shikonin’s antitumor mechanism and therapeutic potential.
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）