查找使用阿拉丁产品的科研文献

Three-in-One: Molecular Engineering of D–A–π–A Featured Type I and Type II Near-Infrared AIE Photosensitizers for Efficient Photodynamic Cancer Therapy and Bacteria Killing

Yun-Yun Quan,Tingting Pan,Zhongda Zhang,Shihua Wang,Guiyun Wang,Lichao Yu,Ye Wang,Xu-Feng Zang,Fangjun Zhang,Xiaoxia Ye,Xuebo Pan,Zu-Sheng Huang

Bacterial infections are closely correlated with the genesis and progression of cancer, and the elimination of cancer-related bacteria may improve the efficacy of cancer treatment. However, the combinatorial therapy that utilizes two or more chemodrugs will increase potential adverse effects. Image-guided photodynamic therapy is a highly precise and potential therapy to treat tumor and microbial infections. Herein, four donor–acceptor–π-bridge–acceptor (D–A–π–A) featured near-infrared (NIR) aggregation-induced emission luminogens (AIEgens) ( TQTPy , TPQTPy , TQTC , and TPQTC ) with type I and type II reaction oxygen species (ROS) generation capabilities are synthesized. Notably, TQTPy shows mitochondria targeted capacity, the best ROS production efficiency, long-term tumor retention capacity, and more importantly, the three-in-one fluorescence imaging guided therapy against both tumor and microbial infections. Both in vitro and in vivo results validate that TQTPy performs well in practical biomedical application in terms of NIR-fluorescence imaging-guided photodynamic cancer diagnosis and treatment. Moreover, the amphiphilic and positively charged TQTPy is able to specific and ultrafast discrimination and elimination of Gram-positive (G+) Staphylococcus aureus from Gram-negative (G−) Escherichia coli and normal cells. This investigation provides an instructive way for the construction of three-in-one treatment for image-guided photodynamic cancer therapy and bacteria elimination.

Development of a pair of differential H/D isotope-coded derivatization reagents d0/d3-4-(1-methyl-1H-phenanthro[9,10-d]imidazol-2-yl)phenlamine and its application for determination of aldehydes in selected aquatic products by liquid chromatography–tandem mass spectrometry

Zhiwei Sun,Xiaoxiang Wang,Yiping Cai,Junqing Fu,Jinmao You

A new pair of derivatization reagents, d 0 -4-(1-methyl-1 H -phenanthro[9,10- d ]imidazol-2-yl)phenlamine (d 0 -MPIA) and d 3 -4-(1-methyl-1 H -phenanthro[9,10- d ]imidazol-2-yl)phenlamine (d 3 -MPIA) have been designed and synthesized. It was successfully used to label aliphatic aldehydes and the aldehyde derivatives were analyzed by high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS). The new isotope-coded reagents could easily label aldehydes under acidic conditions in the presence of NaCNBH 3 . The target derivatives exhibited intense [M+H] + and regular product ions with electrospray ionization source in positive mode. The d 0 /d 3 -MPIA-aldehydes were monitored by the transitions of [M+H] + → m/z 322 and [M+H] + → m/z 165, and the obtained detection limits were in the range of 0.18–15.9   pg/mL at signal to noise ratio of 3. The global isotope internal standard technology was employed for quantification analysis with d 3 -MPIA-aldehyde as internal standard for corresponding d 0 -MPIA-aldehyde. Excellent linear responses for relative quantification were observed in the range of 1/10–10/1 with coefficients >0.998. The developed method has been applied to the quantification of aliphatic aldehydes in selected aquatic products with RSD<3.6% and recoveries >85.2%.

Mussel polysaccharide α-D-glucan (MP-A) protects against non-alcoholic fatty liver disease via maintaining the homeostasis of gut microbiota and regulating related gut-liver axis signaling pathways

Jixu Wu,Huarong Shao,Jinhua Zhang,Yong Ying,Yanling Cheng,Dan Zhao,Xixi Dou,Huimin Lv,Shuaiguang Li,Fei Liu,Peixue Ling

We isolated and characterized a Mussel polysaccharide, α -D-glucan (MP-A), from Mytilus coruscus earlier. In this work, the pharmacological activity and mechanisms of MP-A as an oral supplement for non-alcoholic fatty liver disease (NAFLD) were explored. High fat diet (HFD) was utilized to induce NAFLD in Sprague Dawley male rats and MP-A (0.6 g/kg) was supplemented for 4 weeks. The results showed that MP-A supplementation reduced blood lipid levels, intrahepatic lipid accumulation and NAFLD activity score in HFD-fed rats. Additionally, the analysis of 16S rDNA sequencing on gut microbiota samples revealed that HFD could induce microbial dysbiosis. However, MP-A supplementation could remodel gut microbiota structure, inhibit LPS-TLR4-NF- κ B pathway activation, and restrain subsequent inflammation factors secretion. Furthermore, MP-A regulated the lipid metabolism by promoting the production of short chain fatty acids and suppressing PPAR γ and SREBP-1c expression. Our results support that MP-A can prevent against NAFLD and act as an oral supplementation for hepatoprotection via modulating gut microbiota and related gut-liver axis signaling pathways.

A phenanthro[9,10-d]imidazole-based AIE active fluorescence probe for sequential detection of Ag+/AgNPs and SCN− in water and saliva samples and its application in living cells

Fanqiang Bu,Bing Zhao,Wei Kan,Liyan Wang,Bo Song,Jianxin Wang,Zhe Zhang,Qigang Deng,Guangming Yin

Ag + and SCN − play extremely important roles in the fields of the physiology and environment. In this work, on the basis of phenanthro[9,10- d ]imidazole derivative ( DIPIP ) which can exhibit the aggregation-induced emission (AIE) properties in aqueous solution, we achieved a sequential on-off-on switch for Ag + and SCN − with high selectivity and sensitivity. A remarkable fluorescence quenching effect of Ag + on the probe DIPIP was observed with 1:2 stoichiometry , Subsequently, the fluorescence intensity of in situ generated DIPIP -Ag + ensemble was easily switched on after the interaction between Ag + and SCN − , which was attributed to the stronger affinity of SCN − to capture Ag + . In particular, the extreme limits of detection (LOD) for Ag + and SCN − in standard solutions were as low as to be 74.5 nM and 7.8 nM, respectively. Furthermore, the probe DIPIP and the DIPIP -Ag + ensemble could be used to detect Ag + in the real water and SCN − in smoker saliva samples, respectively. In addition, the sequential “on-off-on” fluorescence mode of DIPIP to Ag + and SCN − were also successfully applied in living HeLa cells.

Chromatographic study on the high performance separation ability of a homochiral [Cu2(d-Cam)2(4,4′-bpy)]n based-column by using racemates and positional isomers as test probes

Mei Zhang,Jun-Hui Zhang,Yan Zhang,Bang-Jin Wang,Sheng-Ming Xie,Li-Ming Yuan

This study focused on exploring the chromatographic characteristic of a homochiral MOF [Cu 2 ( d -Cam) 2 (4,4′-bpy)] n with 3-D six-connected self-penetrating architectures for the separations of positional isomers and chiral compounds. Some effects such as mobile phase composition, column temperature, and analytes mass for separations on this chiral column also have been investigated. Among thirteen tested racemates, six compounds, namely three alcohols, a diol, a naphthol and a ketone were successfully discriminated, and ten useful positional isomers which have never been separated on other MOFs stationary phases in HPLC except dichlorobenzene and cresol isomers, were also analyzed by this chiral stationary phase. The results indicated that [Cu 2 ( d -Cam) 2 (4,4′-bpy)] n exhibited good selectivity for positional isomers and enantiomers. Therefore, it is practicable that the separation of positional isomers and enantiomers are carried out on the chiral MOF column.

Construction of Fully Biodegradable Poly(L-Lactide-Co-Caprolactone) Random Copolymer/Poly(D-Lactide) Blend: Crystallization, Toughness, and Biodegradability

Lei Gong,Yuesheng Zhang,Enyang Xu,Hongtao Lu,Junrui Duan,Jianna Bao

In this work, poly(L-lactide-co-ε-caprolactone) (PLCL) copolymers with varying ε-caprolactone (ε-CL) contents that ranged from 0% to 10% were synthesized via ring-opening polymerization of L-lactide and ε-CL at 170°C for 1 h. Fully biodegradable PLCL/poly(D-lactide) (PDLA) blends with stereocomplex (SC) crystallites were fabricated through a solution blending approach. The effects of ε-CL content in PLCL on the crystallization behavior, thermal properties, mechanical performance, and degradation behavior of PLCL/PDLA blends were systematically investigated. The successful synthesis of PLCL was confirmed by 1 H NMR and FTIR analyses. The melting peaks observed in the high-temperature region and the characteristic diffractions in WAXD patterns provided evidence for the formation of SC crystallites in PLCL/PDLA blends. Compared with PLCL, the incorporation of PDLA significantly enhanced the crystallization rate, due to the nucleation function of SC crystallites. With increasing ε-CL content in the copolymer system, the T g and crystallinity of PLCL gradually reduced, and the melting peaks of HC and SC crystals shifted toward lower temperatures due to the reduced sequence regularity. A pronounced improvement in deformability was achieved by the presence of ε-CL segments. As the ε-CL content increased from 1.5% to 10%, the elongation at break rose from 12% to 192%, with the morphology of elongated fibrils. Higher ε-CL contents accelerated enzymatic degradation, with mass loss exceeding 75% after 7 days at 10% ε-CL, because the increased chain mobility may facilitate the penetration of water and Proteinase K into the blend, thereby promoting enzymatic erosion. Overall, high-performance PLA-based composites with excellent biodegradability were achieved via SC crystallization and modulation of ε-CL comonomer content. Summary PLCL copolymers with tunable ε-CL content were prepared and characterized. SC crystallization regulated the crystallization and thermal behavior. ε-CL segments enhance chain mobility and improve blend deformability. Enzymatic degradation kinetics were accelerated as ε-CL content increased.

Tuning the d-Band center of High-Entropy oxides for selective aromatic oxidation and viscosity reduction in heavy oil

Lian Chen,Zhaofei Ma,Yu Jiang,Yuanzhu Mi,Yancheng Zheng,Xuemin Yan

Despite abundant global reserves, the industrial utilization of heavy oil is severely limited by its extremely high viscosity, primarily caused by the aggregation of polycyclic aromatic hydrocarbons (PAHs). In this study, we propose a novel viscosity reduction strategy using spinel-structured high-entropy oxides (HEOs) as low-temperature catalysts requiring no additional oxidants, representing a significant departure from conventional thermal and oxidative methods. By tuning the d-band centers of multicomponent metal sites, the electronic structure of HEOs is optimized to selectively activate and moderately oxidize aromatic-rich asphaltenes. This catalytic process disrupts π–π stacking and introduces oxygen-containing functional groups, triggering a fundamentally new, irreversible viscosity reduction mechanism driven by molecular fragmentation and polarity modulation. Experimental validations, including rheological measurements, spectroscopic analysis, and model compound studies, reveal a substantial viscosity reduction of ∼ 75.05%. Mechanistically, the partial oxidation of asphaltenes promotes molecular disorder, releases trapped light components from the condensed matrix, and facilitates the penetration of surfactants, thereby lowering the oil–water interfacial tension. Overall, this work establishes HEOs as a next-generation catalytic platform and presents a new pathway for efficient and sustainable heavy oil mobility enhancement.

MXene quantum dots induced Ni site d-band center self-optimization for advanced photovoltaic-driven urea-assisted water splitting

Yufeng Jiang,Shaobin Li,Li Zhang,Yang Yang,Kun Cheng,Fengbo Li,Xiaoqing Lv

Urea-assisted electrolytic water splitting for hydrogen production represents an effective contemporary strategy for generating green hydrogen. The sluggish kinetics of the anodic urea oxidation reaction (UOR) and cathodic hydrogen evolution reaction (HER) limit their practical application. The MXene quantum dots (MQDs) promote pronounced d-d orbital hybridization between Ni and Mo sites. The constructed heterostructures facilitate electron transfer and modulate charge redistribution. The extensive formation of heterostructures between numerous quantum dots and nickel molybdate results in the emergence of flower-like clusters at the tips of rod-like NiMoO 4 particles. The augmentation of the specific surface area results in the exposure of a larger quantity of active sites, which facilitates the redistribution of charge across these active sites, consequently enhancing the hydrolytic activity. The electrocatalyst exhibits remarkable performance in both the UOR and HER, achieving 1.31 V and 55 mV at 10 mA cm –2 , respectively. The catalyst for urea–assisted water electrolysis requires only 1.38 V at 10 mA cm –2 . The prepared catalyst can be employed for seawater splitting and photovoltaic-driven hydrogen production. In-situ Raman spectrum confirms that Ni sites on the NiMoO 4 /MQDs surface transform into Ni–OOH under hydrolysis and subsequently govern the hydrolytic reaction. This work indicates that d-d orbital hybridization can provide pathways for electronic transitions, thereby enhancing charge-transfer efficiency and effectively lowering reaction energy barriers.

Honokiol-Loaded β-D-Glucan/Chitosan Nanoparticles with Antibacterial and Anti-Inflammatory Activities for Promoting MRSA-Infected Wound Healing

Background:Methicillin-resistantStaphylococcus aureus(MRSA) wound infections are a major clinical concern due to their high prevalence and the growing risk of antimicrobial resistance. Therefore, it is imperative to develop safe and effective therapeutic strategies.Methods: Herein, we fabricated a novel antibiotic-free nanodrug delivery system (HNK/DG-COS NPs) by loading honokiol (HNK), a hydrophobic natural antimicrobial agent, onto a dual-polysaccharide carrier composed of chitosan (COS) and β-D-glucan (DG), for the treatment of MRSA-infected wounds. COS endows the nanoparticles with synergistic antibacterial activity together with HNK, while DG improves their anti-inflammatory and tissue repair capacities. The physicochemical characteristics of nanoparticles, as well as their in vitro antibacterial and anti-inflammatory activities, were systematically evaluated. Furthermore, an MRSA-infected wound model was constructed to verify their therapeutic efficacy.Results: HNK/DG-COS NPs exhibited a spherical morphology with a uniform particle size of approximately 200 nm. In vitro experiments have shown that HNK/DG-COS NPs can significantly kill MRSA and inhibit biofilm formation. Scanning electron microscopy further revealed that the nanoparticles exerted antibacterial effects by disrupting the integrity of bacterial cell membranes. In LPS-stimulated RAW264.7 macrophages, treatment with HNK/DG-COS NPs significantly mitigated pro-inflammatory cytokine levels. Cell migration assays confirmed that nanoparticles effectively enhanced L929 cell migration. In vivo, treatment with HNK/DG-COS NPs significantly accelerated MRSA-infected wound healing in mice, mitigated inflammatory damage, stimulated collagen production and repaired damaged tissues.Conclusions: HNK/DG-COS NPs represent a promising antibiotic-free candidate for intervention against early-stage MRSA-infected wounds.

Quantum size–induced orbital splitting drives d–p hybridization for enhanced hydrogen evolution in anion-exchange membrane water electrolyzers

Hongxu Su,Shijie Liu,Sicheng Geng,Xu Xu,Xiao Wang,Qiong Gao,Mingyi Zhang,Xinzhi Ma,Lin Li

Electronic modulation in quantum-size catalysts plays a critical yet underexplored role in governing the hydrogen evolution reaction (HER) in anion-exchange membrane water electrolyzers (AEMWEs). Here, cobalt nanoparticles with tunable diameters (5–15 nm) encapsulated in nitrogen-doped carbon nanotubes (Co@NCNTs) were engineered to elucidate how size-dependent orbital evolution dictates HER activity in alkaline media. We found that a smaller particle diameter results in a better charge transfer capability. Besides, the in-situ Raman spectroscopy shows that Co@NCNTs with an optimized diameter preserve a stable Co–N–C vibration at 690 cm −1 throughout HER, while transiently forming surface C–OH species—key intermediates that promote water dissociation. Inverse and ultraviolet photoelectron spectroscopy (IPES/UPS) further reveal that the quantum-size-effect splits the d -band and gives rise to a stronger Co 3 d –O 2 p hybridization. This enhanced hybridization facilitates electronic delocalization across Co–N–C/O interfaces, enabling dynamic charge transfer that favors the formation and subsequent reduction of C–OH intermediates during HER. As a result, the optimized Co@NCNTs catalyst achieves an overpotential of only 33 mV at 10 mA cm −2 and delivers 550 h operational stability in a full AEMWE. These findings provide direct experimental evidence that quantum-size-tailored orbital coupling governs interfacial OH/H 2 , thereby linking spectroscopic insight with catalytic function and establishing a rational design principle for high-performance non-noble-metal catalysts.

Phosphorus-induced d-p orbital hybridization in PtFeCoCuMo alloys: Regulating active hydrogen supply to enhance alkaline hydrogen evolution reaction

Zixuan Deng,Shuang Zhang,Fudong Li,Yue Zhu,Changsheng Shan,Yuanmeng Zhao

Rationally designing electrocatalyst/electrolyte interfaces with excellent active hydrogen supply capability is crucial for the hydrogen evolution reaction (HER), a core step in alkaline anion exchange membrane water electrolysis (AEMWE). Herein, phosphorus (P)-surface-coordinated PtFeCoCuMo quinary alloys are synthesized via a two-step wet-chemical method. P-induced d-p orbital hybridization enables the synergistic regulation of two key factors: it optimizes the hydrogen adsorption free energy (ΔG H∗ ) and water dissociation barrier (thermodynamic modulation), while tailoring the potential of zero charge (PZC) to accelerate proton transport (kinetic modulation). The optimized P 16 -PtFeCoCuMo catalyst exhibits outstanding alkaline HER activity, with a turnover frequency (TOF) 5-fold and 3-fold higher than those of commercial Pt/C and pristine PtFeCoCuMo alloy, respectively. This work overcomes thermodynamic and kinetic limitations simultaneously through heteroatom doping, providing a promising strategy for the design of high-performance alkaline HER catalysts.

Hyaluronic acid-ethylenediamine-cinnamic acid attenuates IBS-D via regulating 5-hydroxytryptamine signaling pathway, intestinal barrier and gut microbiota

Li Cui,Rongling Zhong,Wanqiu Peng,Zhenhai Zhang,Zhentao An,Hui Li

Current clinical management of diarrhea-predominant irritable bowel syndrome (IBS-D) faces substantial challenges due to the limited efficacy of existing therapeutic strategies. This study aimed to synthesize and characterize the hyaluronic acid-ethylenediamine-cinnamic acid conjugate (HA-EDA-CA), investigate its oral bioavailability and colonic tissue distribution, and validate its therapeutic potential along with underlying mechanisms of action in IBS-D models. Following oral administration of free CA or HA-EDA-CA, the colon concentration of CA released from the derivative was significantly higher than that of free CA alone. Both free and conjugated forms of CA likely contributed to the therapeutic effects against IBS-D. The IBS-D model was established by a combination of senna decoction administration and physical restraint stress. After HA-EDA-CA intervention, IBS-D model mice showed marked improvements in stool consistency, fecal water content, stool output, body weight, visceral hypersensitivity, and colon length. ELISA results indicated significant reduction in enteric neurotransmitter levels, including 5-hydroxytryptamine, substance P, and vasoactive intestinal peptide, after oral gavage administration of HA-EDA-CA. Additionally, intestinal permeability assessment and immunofluorescence analyse revealed that HA-EDA-CA improved intestinal barrier function. Western blot analysis indicated HA-EDA-CA inhibited 5-hydroxytryptamine signaling pathway. Furthermore, HA-EDA-CA modulated gut microbiota composition. These findings suggested the potential of HA-EDA-CA as a promising therapeutic candidate for IBS-D treatment. Key novelties: (i) First colon-targeted CA-HA conjugate: We developed and characterized the first hyaluronic acid-based conjugate specifically designed for colon-targeted delivery of cinnamic acid. (ii) Multi-mechanism rescue: The conjugate simultaneously restores gut homeostasis by modulating the 5-hydroxytryptamine signaling pathway, enhancing intestinal barrier function, and rebalancing gut microbiota. (iii) Enhanced therapeutic efficacy: HA-EDA-CA demonstrates superior colonic drug accumulation and integrated therapeutic outcomes compared to free cinnamic acid or simple drug mixtures. (iv) Open-source data availability: All relevant data and analysis scripts supporting the findings are publicly accessible to ensure reproducibility and further research.