JOURNAL OF FOOD SCIENCE
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2019
·Vol. 84
·期 5
·936-945
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IF 2.081
Dantong Wang,Yudi Ma,Qiang Wang,Juan Huang,Rui Sun,Qiang Xia
The present study was aimed at formulating and evaluating a novel solid self-emulsifying delivery system (S-SEDS) for the application in functional foods of dihydromyricetin (DMY). First, solubility study and pseudo-ternary phase diagram analysis were adopted to optimize the formulation of liquid self-emulsifying delivery system (L-SEDS). And the thermodynamic stable L-SEDS with 5% content of DMY was fabricated and further developed into a solid form via vacuum rotary evaporation with Aerosil 300 as the solid adsorbent. Solid state characterization of the S-SEDS was performed by scanning electron microscopy, Fourier-transform infrared spectroscopy, and X-ray powder diffraction. Furthermore, studies proved that the antioxidant activity and bioaccessibility of DMY were improved after incorporated into S-SEDS formulation compared to pure DMY. The S-SEDS showed good resistance against various storage conditions investigated for 10 weeks. Practical Application Solid self-emulsifying delivery system (S-SEDS) combined the advantages of liquid self-emulsifying delivery system with those of a solid dosage form to overcome the disadvantages associated with liquid formulations is more convenient for storage and transportation in practical application. Furthermore, the technology of producing S-SEDS is simple and can be realized in industrial production. Hence, S-SEDS could be a promising strategy to overcome the poor water solubility and short biological half-life of dihydromyricetin for further application in functional foods and beverage industry.
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ELECTROCHIMICA ACTA
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2018
·
IF 5.116
Zhenqi Cui,Jia Yao,Tao Mei,Shiyuan Zhou,Baofei Hou,Jing Li,Jinhua Li,Jianying Wang,Jingwen Qian,Xianbao Wang
In our study, TiC-TiO 2 /S composite was successfully prepared with a simple and effective method. For this special micro-mesoporous structure, TiC-TiO 2 can be used as an efficient sulfur storing material, and also restrain the volume expansion of sulfur to a certain degree for its suitable specific surface area. TiO 2 and TiC can suppress the diffusion of polysulfides due to their strong interaction with polysulfides . At the same time, TiC also can remedy the nonconductive defect of sulfur for its good conductivity. With the help of above advantages, the TiC-TiO 2 /S cathode delivers an initial discharge specific capacity of 1218 mAh g −1 at 1 C (1 C = 1675 mA g −1 ) with the sulfur loading of 1.5 mg cm −2 . And when cycles at 0.5 C, a stable capacity of 714 mAh g −1 can be maintained after 500 cycles accompanied with the sulfur loading of 1.1 mg cm −2 .
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BIOTECHNOLOGY LETTERS
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2019
·Vol. 41
·期 10
·1223-1232
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IF 2.154
Zhang Yin-Jun,Chen Chang-Sheng,Liu Hao-Tian,Chen Jia-Lin,Xia Ying,Wu Shi-Jin
Objective To purify an esterase which can selectively hydrolyze ( R,S )-ethyl indoline-2-carboxylate to produce ( S )-indoline-2-carboxylic acid and characterize its enzymatic properties. Results An intracellular esterase from Bacillus aryabhattai B8W22 was isolated and the purified protein was identified as a carboxylesterase by MALDI-TOF mass spectrometry. The enzyme (named BaCE) was 59.03-fold purification determined to be of approximately 35 kDa. Its specific activity was 0.574 U/mL with 20% yield. The enzyme showed maximum activity at pH 8.5 and 30 °C and was stable at 20–30 °C using p NPB as the substrate. The K m , V max , k cat and k cat /K m of the esterase were 0.52 mM, 6.39 μM/min, 26.87 min −1 and 51.67 mM/min, respectively. The esterase demonstrated high enantioselectivity toward ( S )-ethyl indoline-2-carboxylate with 96.55% e.e. p at 44.39% conversion, corresponding to an E value of 133.45. Conclusions In this study, a new esterase BaCE with an apparent molecular mass of 35 kDa was purified to homogeneity for the first time. The esterase from Bacillus aryabhattai B8W22 was isolated with a purification more than 59-fold and a yield of 20% by anion exchange chromatography and hydrophobic interaction chromatography. And its biochemical characterization were described in detail with p NPB as substrate. It displayed high enantioselectivity toward ( S )-ethyl indoline-2-carboxylate. We next plan to highly express esterase BaCE in Escherichia coli , and apply it to industrial production of ( S )-indoline-2-carboxylic acid.
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WATER RESEARCH
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2019
·
IF 7.913
Xiaohui Lu,Jingnan Zhao,Qun Wang,Da Wang,Haodan Xu,Jun Ma,Wei Qiu,Tao Hu
In this paper, the kinetics of bisphenol S (BPS) degradation in the presence of peroxydisulfate (PDS) or dissolved oxygen (DO) in ultrasound (US) system were investigated. For PDS (US/PDS), increased PDS concentration result in faster BPS degradation, but the enhancement was not remarkable with multiplying PDS dosages. Therefore, heterogeneous PDS activation model based on a Langmuir-type adsorption mechanism was proposed to explain the trait of BPS abatement. The equilibrium constant of PDS (K PDS ) was calculated to be 2.91 × 10 −4 /μM, which was much lower than that of BPS, suggesting that PDS was hard to adsorb on the gas-liquid interface of the cavitation bubble following by activation. Besides, the formation of •OH and SO 4 •− in US/PDS system was reinvestigated. The result showed that SO 4 •− rather than •OH was the predominant radical, which was quite different from previous study. Dissolved oxygen largely improve the degradation of BPS in US system and •OH rather than O 2 •− was proved to be the main reactive oxygen species (ROS). The improvement of •OH generation possibly caused by the reaction of DO with •H so that it cannot recombine with •OH. The transformation of the BPS in US system mainly included BPS radical polymerization, hydroxylation and hydrolysis. Frustratingly, the acute toxicity assay of Vibrio fischeri suggests that the degradation products of BPS are more toxic. These results will improve the understanding on the activation mechanisms of PDS and the role of dissolved oxygen play in US. Further investigations may need to explore other treatment ways of BPS and evaluate the acute toxicity of degradation products.
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CHEMICAL ENGINEERING JOURNAL
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2019
·
IF 8.355
Wenyan Du,Yuanke Wu,Tingting Yang,Bingshu Guo,Dingyu Liu,Shu-Juan Bao,Maowen Xu
Developing high energy density room-temperature sodium sulfur (RT Na-S) batteries relies on the design of delicate structure that can be efficiently injected sulfur and enhance the cycle life of electrode active material. Herein, a three dimensional (3D) hierarchical cathode substrate with VO 2 nanoflowers as catalyst in situ grown on reduced graphene oxide (rGO) for sulfur cathodes are designed and prepared. In this novel structure, the electronic conductivity of sulfur cathode can be greatly improved due to the high electrical conductivity of rGO substrate. More importantly, the catalytic effect of VO 2 accelerates the conversion long-chain NaPSs to Na 2 S 2 /Na 2 S, and enhances the cycle capability, which can be validated by experimental data. As a result, the as-obtained rGO/VO 2 /S composites achieves an initial reversible capacity of 876.4 mA h g −1 at 0.2C. Moreover, the capacity of 156.1 mA h g −1 is demonstrated after long-cycling term of 1000 cycles at 2C and high cycle stability of only 0.07% capacity decay per cycle is exhibited.
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ACS Nano
·
2019
·
IF 13.903
Wei Kou,Xiangcun Li,Yang Liu,Xiaopeng Zhang,Shaoran Yang,Xiaobin Jiang,Gaohong He,Yan Dai,Wenji Zheng,Guihua Yu
Here we report a highly scalable yet flexible triple-layer structured porous C/SiO2 membrane via a facile phase inversion method for advancing Li–sulfur battery technology. As a multifunctional current-collector-free cathode, the conductive dense layer of the C/SiO2 membrane offers hierarchical macropores as an ideal sulfur host to alleviate the volume expansion of sulfur species and facilitate ion/electrolyte transport for fast kinetics, as well as spongelike pores to enable high sulfur loading. The triple-layer structured membrane cathode enables the filling of most sulfur species in the macropores and additional loading of a thin sulfur slurry on the membrane surface, which facilitates ion/electrolyte transport with faster kinetics than the conventional S/C slurry-based cathode. Furthermore, density functional theory simulations and visual adsorption measurements confirm the critical role of the doped SiO2 nanoparticles (∼10 nm) in the asymmetric C membrane in suppressing the shuttle effect of polysulfides via chemisorption and electrocatalysis. The rationally designed C/SiO2 membrane cathodes demonstrate long-term cycling stability of 300 cycles at a high sulfur loading of 2.8 mg cm–2 with a sulfur content of ∼75%. This scalable yet flexible self-supporting cathode design presents a useful strategy for realizing practical applications of high-performance Li–S batteries.
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Advanced Energy Materials
·
2019
·Vol. 9
·期 14
·1803935
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IF 24.884
Yanyan Liu,Miaomiao Han,Qizhong Xiong,Shengbo Zhang,Cuijiao Zhao,Wanbing Gong,Guozhong Wang,Haimin Zhang,Huijun Zhao
The Haber-Bosch process can be replaced by the ambient electrocatalytic N 2 reduction reaction (NRR) to produce NH 3 if suitable electrocatalysts can be developed. However, to develop high performance N 2 fixation electrocatalysts, a key issue to be resolved is to achieve efficient hydrogenation of N 2 without interference by the thermodynamically favored hydrogen evolution reaction (HER). Herein, in-operando created strong Li–S interactions are reported to empower the S-rich MoS 2 nanosheets with superior NRR catalytic activity and HER suppression ability. The Li + interactions with S-edge sites of MoS 2 can effectively suppress hydrogen evolution reaction by reducing H* adsorption free energy from 0.03 to 0.47 eV, facilitate N 2 adsorption by increasing N 2 adsorption free energy from –0.32 to –0.70 eV and enhance electrocatalytic N 2 reduction activity by decreasing the activation energy barrier of the reaction control step (*N 2 → *N 2 H) from 0.84 to 0.42 eV. A NH 3 yield rate of 43.4 μg h −1 mg −1 MoS 2 with a faradaic efficiency (FE) of 9.81% can be achieved in presence of strong Li–S interactions, more than 8 and 18 times by the same electrocatalyst in the absence of Li–S interactions. This report opens a new way to design and develop catalysts and catalysis systems.
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International Journal of Nanomedicine
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2019
·
IF 4.471
Weiguang Li,Jianpeng Xue,Hanmei Xu
Background S-HM-3 is a tumor angiogenesis inhibitor with short half-life (25 min). In this present, TPGS/Solutol polymeric micelles was prepared to load together insoluble paclitaxel (PTX) and soluble S-HM-3, expecting to together deliver them to the tumor site with long-circulating, targeting function and combating multi-drug resistance (MDR). Materials and methods PTX and S-HM-3 loaded TPGS/Solutol micelles (PHTSm) were prepared by the method of thin-film evaporation, and characterized by dynamic light scattering, transmission electron microscope (TEM), atomic force microscopy (AFM) and releasing properties. The anticancer effect of the polymeric micelles system was evaluated and confirmed by experiments of in vitro cell uptake study, in vivo pharmacokinetics, and pharmacodynamics studies. Results Micelles exhibited smooth spherical morphology with 20~30 nm and low critical micelle concentration (CMC) value of 0.000124 mg/mL. Only about 30% of PTX were slowly released from micelles at 48h, which can beneficial to the long circulation in blood. The results of in vitro cell assay proved that S-HM-3 could be easier to get into MDA-MB-231 cell, and its angiogenesis inhibition ability was also enhanced after integrating into micelles. In particular, the results of in vivo studies showed that the half-life of S-HM-3 and PTX was significantly prolonged 25.27 and 5.54 folds, and their AUC 0–∞ was enhanced 129.78 and 15.65 times, respectively. Meanwhile 83.05% tumor inhibition rate of PHTSm was achieved compared with 59.99% of PTX. Conclusions TPGS and Solutol micelles hold promising potential to resolve the conundrum of combined therapy of cytotoxic drug and angiogenesis inhibitor with different physicochemical property and anticancer mechanism in clinical use.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2019
·Vol. 411
·期 8
·1647-1657
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IF 3.286
Zuo Pengli,Liu Jianhua,Guo Hongna,Wang Chenghong,Liu Hongqian,Zhang Zhigang,Liu Qingyou
In this paper, we have presented a facile method to fabricate nitrogen and sulfur co-doped carbon dots (N,S-CDs) for blood methotrexate (MTX) sensing applications. The N,S-CDs with quantum yield up to 75% were obtained by one-step hydrothermal carbonization, using reduced glutathione and citric acid as the precursors. With this approach, the formation and the surface passivation of N,S-CDs were carried out simultaneously, resulting in intrinsic fluorescence emission. Owing to their pronounced temperature dependence of the fluorescence emission spectra, resultant N,S-CDs can work as versatile nanothermometry devices by taking advantage of the temperature sensitivity of their emission intensity. In addition, the obtained N,S-CDs facilitated high selectivity detection of Fe 3+ ions with a detection limit as low as 0.31 μM and a wide linear range from 3.33 to 99.90 μM. More importantly, the added MTX selectively led to the fluorescence quenching of the N,S-CDs. Such fluorescence responses were used for well quantifying MTX in the range of 2.93 to 117.40 μM, and the detection limit was down to 0.95 μM. Due to “inert” surface, the N,S-CDs well resisted the interferences from various biomolecules and exhibited excellent selectivity. The proposed sensing system was successfully used for the assay of MTX in human plasma. Due to simplicity, sensitivity, selectivity, and low cost, it exhibits great promise as a practical platform for MTX sensing in biological samples.
Graphical Abstract
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JOURNAL OF ORGANOMETALLIC CHEMISTRY
·
2018
·
IF 1.946
Amin Khan,Li Wang,Haojie Yu,Muhammad Haroon,Raja Summe Ullah,Ahsan Nazir,Tarig Elshaarani,Muhammad Usman,Shah Fahad,Kaleem-ur-Rehman Naveed
Three novel polymers (P(FHEMA- co -MAAT)s) having redox and photoresponsive moieties were prepared by free radical polymerization . The structures and molecular weights of the synthesized copolymers were characterized by nuclear magnetic resonance ( 1 H NMR) and gel permeation chromatography (GPC) respectively. Thermal properties, optical features and electroactive properties were studied with the help of thermogravimetric (TG), differential thermogravimetric (DTG) analysis, ultraviolet-visible spectroscopy (UV-vis) and cyclic voltammetry (CV). It was seen that synthesized copolymers had excellent redox and photoresponsive properties. These results exhibited that these polymers can be applied further for redox/photo responsive information storage devices.
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PEST MANAGEMENT SCIENCE
·
2019
·Vol. 75
·期 10
·2802-2809
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IF 3.255
Kaiyang Feng,Yuwei Yang,Xiang Wen,Shiyuan Ou,Ping Zhang,Qian Yu,Yichao Zhang,Guangmao Shen,Zhifeng Xu,Jinhang Li,Lin He
BACKGROUND Cyflumetofen is an outstanding acaricide with a novel mode of action. Tetranychus cinnabarinus , an important agricultural pest, is notorious for developing resistance to most classes of acaricides rapidly and results in enormous loss for the economy. Our previous study had pointed out glutathione S-transferase (GSTs) significantly contributed to the cyflumetofen-resistance formation in T. cinnabarinus , but the more specific mechanism needed to be further investigated. RESULTS The unstable resistance was observed in cyflumetofen-resistant strain (CyR)under acaricide-free condition. The activity of GSTs increased along with the development of resistance. The expressions of 13 GST genes were detected in CyR and susceptible strain (SS), of which six genes were overexpressed in CyR and the TcGSTm02 was selected as the representative for functional study. The expression of TcGSTm02 changed along with the resistant level of CyR with the same trend. Recombinant protein of TcGSTm02 with high activity was successfully obtained by E. coli expression system, whose activity could be inhibited by cyflumetofen (IC 50 = 0.23 mM). Recombinant TcGSTm02 could effectively decompose cyflumetofen, and catalyze GS − to conjugate with cyflumetofen. CONCLUSION All clues confirmed that GSTs strongly associated with cyflumetofen-resistance and a representative gene, TcGSTm02 , showed function on contributing the evolution of cyflumetofen-resistance in T. cinnabarinus . © 2019 Society of Chemical Industry
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Nano Research
·
2018
·Vol. 11
·期 3
·1379-1388
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IF 7.994
Liu Xiao-Yuan,Zhang Guozhen,Chen Hao,Li Haowen,Jiang Jun,Long Yi-Tao,Ning Zhijun
The development of photocatalysts that can effectively harvest visible light is essential for advances in high-efficiency solar-driven hydrogen generation. Herein, we synthesized water soluble CuInS 2 (CIS) and Cu-In-Zn-S (CIZS) quantum dots (QDs) by using one-pot aqueous method. The CIZS QDs are well passivated by glutathione ligands and are highly stable in aqueous conditions. We subsequently applied these QDs as a light harvesting material for photocatalytic hydrogen generation. Unlike most small band gap materials that show extremely low efficiency, these new QDs display remarkable energy conversion efficiency in the visible and near-infrared regions. The external quantum efficiency at 650 nm is ∼1.5%, which, to the best of our knowledge, is the highest value achieved until now in the near-infrared region.
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危险品化学品经营许可证（带存储）