ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Yanping Zhao,Xu Geng,Xiaoling Zhou,Li Xu,Shuai Li,Zhengqiang Li,Yi Guo,Chen Li
Pyruvate participates in diverse metabolic pathways in the body and is normally present in human blood at 40–120 μM, with concentrations outside this range associated with various diseases. Therefore, accurate and stable blood pyruvate level tests are necessary for effective disease detection. However, traditional analytical techniques require complicated instrumentation and are time consuming and expensive, prompting researchers to develop improved methods based on biosensors and bioassays. Here, we designed a highly stable bioelectrochemical pyruvate sensor affixed to a glassy carbon electrode (GCE). To maximize biosensor stability, 0.1 U of lactate dehydrogenase was affixed to the GCE using a sol-gel process, resulting in generation of Gel/LDH/GCE. Next, 2.0 mg/mL AuNPs-rGO was added to enhance current signal strength, resulting in generation of the bioelectrochemical sensor Gel/AuNPs-rGO/LDH/GCE. AuNPs-rGO synthesized in advance was verified as correct using transmission electron microscopy and UV–Vis, Fourier-transform infrared and X-ray photoelectron spectroscopy. Pyruvate detection conducted via differential pulse voltammetry in phosphate buffer (pH 7.4, 100 mM) at 37 °C for 1–4500 μM pyruvate provided detection sensitivity as high as 254.54 μA/mM/cm 2 . The reproducibility, regenerability and storage stability were analyzed with the relative standard deviation of 5 bioeletrochemical sensors detection was 4.60% and biosensor accuracy after 9 cycles was 92%, with accuracy remaining at 86% after 7 days. In the presence of D-glucose, citric acid , dopamine, uric acid and ascorbic acid , the Gel/AuNPs-rGO/LDH/GCE sensor exhibited excellent stability, high anti-interference ability and better performance than conventional spectroscopic methods for detection of pyruvate in artificial serum.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2023
·
IF 6.7
He-Xiang Zhao,Ming-Xin Li,Yan Wang,Yuan-Zhuo Tan,Zi-Xiong Zhou,Yuan-Ru Guo,Qing-Jiang Pan
Utilizing nature resource is one of the most effective ways to achieve sustainability development. In this work, super low-concentration CO 2 in the atmosphere and rich and cheap biomaterial cellulose were used to prepare magnetic bio-composite, cellulose nanofiber/Fe 3 O 4 /CaCO 3 (CFeCa). By capturing CO 2 in air, Ca 2+ was transformed into calcite CaCO 3 , which subsequently coated on cellulose nanofiber (CNF). Fe 3 O 4 with about 50 nm in size were also observed to attach to the CNF. Thus, CNF bridged these two inorganic materials, which together fabricated stable composite with strong mutual coupling. The prepared CFeCa was applied to purify the lead-polluted water as water treatment agent. Its up-taken capacity for Pb 2+ reached as high as 1010 mg·g −1 . Almost 100% removal efficiency was attained, even treating Pb 2+ wastewater in a wide concentration range from 5 to 250 ppm. Meanwhile, CFeCa is stable property during application. After running for 30 cycles, the treated water still met drinkable water standard. More importantly, the recovered lead was also bound on the surface of CNF, which make it easy to be separated from water and avoiding the risk of second pollution. Cheap raw materials and multiple merits of resulted composite offer CFeCa a perspective application as water treatment agent.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Xiaoxiang Hu,Wenjing Liu,Yi Yan,Huaxiang Deng,Yujie Cai
Short-chain dehydrogenase/reductase (SDR) acts as a biocatalyst in the synthesis of chiral alcohols with high optical purity. Herein, we achieved immobilization via crosslinking on novel magnetic metal–organic framework nanoparticles with a three-layer shell structure (Fe 3 O 4 @PDA@Cu (PABA)). The results of scanning electron microscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, X-ray photoelectron spectroscopy, and energy dispersive X-ray spectroscopy confirmed the morphology and cross-linking property of immobilized SDR, which was more durable, stable, and reusable and exhibited better kinetic performance than free enzyme . The SDR and glucose dehydrogenase (GDH) were co-immobilized and then used for the asymmetric reduction of COBE and ethyl 2-oxo-4-phenylbutanoate (OPBE). These finding suggest that enzymes immobilized on novel MOF nanoparticles can serve as promising biocatalysts for asymmetric reduction prochiral ketones into chiral alcohols.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 3.475
Yongxing Li,Pengqian Luan,Liya Zhou,Saiguang Xue,Yihang Liu,Yunting Liu,Yanjun Jiang,Jing Gao
The synthesis or decomposition of complex substances through the combination of metals and enzymes is desirable but still a huge challenge. A kind of yolk-shell structured Co/C@SiO 2 @Ni/C nanocomposites were prepared by the polyhedral ZIF-67@SiO 2 composite as a precursor through the extended stöber method and subsequent carbonization treatment. The SiO 2 as an intermediate protective layer stabilized the morphology and structure of the carbonized products. The metallic nickel nanoparticles were uniformly distributed on the surface of Co/C@SiO 2 @Ni/C for highly specific absorption of His-tagged organophosphohydrolase (OpdA) from cell lysates via affinity, and therefore an integrated nanocatalyst (INC) named OpdA@Co/C@SiO 2 @Ni/C was successfully prepared. The prepared INC was applied to achieve a one-pot chemoenzymatic cascade degradation of organophosphates (OPs) to p -aminophenol (PAP) within 30 min. In addition, the INC proved to be a simple and high-sensitivity nanocatalyst for the detection of methyl-parathion with a low limit of detection (0.30 μM). The INC exhibited improved pH stability, thermal stability, SDS resistance, and storage stability compared to that of free enzymes. When OpdA@Co/C@SiO 2 @Ni/C was reused for 7 cycles, the cascade degradation efficiency of methyl-parathion could retain more than 60 %. This study provides a strategy for constructing a multifunctional nanocatalyst, and further demonstrated its potential in biocatalysis, accurate detection of OPs and remediation of environmental pollution.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Yingying Xie,Guining Lu,Xueqin Tao,Zhen Wen,Zhi Dang
A novel collaborative strategy for enhanced removal of Cr(VI) using nano zero valent iron (nZVI) assisted by schwertmannite (Sch) with two synthesis methods was designed. Batch experiments demonstrated that nZVI/Sch-AP (synthesized by abiotic precipitation of Fe 3+ species) exhibited excellent removal performance for Cr(VI) than nZVI/Sch-CO (synthesized by chemical oxidation of Fe 2+ species). The results indicated that the removal efficiencies of Cr(VI) by nZVI/Sch-AP and nZVI/Sch-CO were highly pH-dependent and achieved to be 99.99% and 98.01% under the optimal conditions of 10 mg L −1 Cr(VI) concentration, a pH of 6.3 and a Fe(0)/Cr(VI) molar ratio of 12. But nZVI/Sch-AP emerged greater k of 0.1097 min −1 than that of nZVI/Sch-CO (0.0485 min −1 ). Humic acid exhibited promotion effect on the Cr(VI) removal in low concentration of 1 mg L −1 . Results of XRD and XPS demonstrated that α-FeOOH was the dominant products in both incubations of nZVI/Sch-AP and nZVI/Sch-CO, accompanied with FeCr 2 O 4 and Cr Fe mixed (oxy)hydroxides, and γ-FeOOH was found alone in the incubations of nZVI/Sch-CO. We proposed a consecutive and simultaneous process involving surface absorption-reduction and co-precipitation/immobilization for the removal. This study provides new insights into the elimination of Cr(VI) from wastewater by nZVI/Sch, especially in acid mine drainage .
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MACROMOLECULAR RESEARCH
·
2019
·Vol. 27
·期 10
·963-973
·
IF 1.758
Zeng Han,Huo Wen Shan,Wang Fang,Ma Ting Mei
Influence of interactions between Laccase and carbon nano-tubes modified by perylene derivative on structural parameters and electrochemical behaviors of immobilized redox protein, was investigated systematically by the means of spectrometry, photography and electro-chemistry. Sophisticated interactions including abutting coordination could result in anomalistic agglomeration of nano-composite with Lac incorporation which displayed better hydrophobicity than free Laccase and high resistance of charge shuttle. It would retard the progress of thermal decomposition of nano-composite and would lead to the formation of composite without emission of fluorescence. Strong binding of Laccase with perylenyl derivative (binding constant: 7.5×10 4 L/mol) would alter the mechanism of electron transferring. T 1 Cu 2+ of Laccase in the status of complexation played the intra-molecular electron relay with mid-wave potential at −135.6 mV and demonstrated undesirable dynamics in electron transferring (7.7×10 −2 s −1 ). The catalytic function in oxygen reduction was prohibited for the abutting coordination and only weak catalytic effect could be achieved in the presence of electron relay. The whole catalytic cycle was limited by the mass transferring of electron mediator.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2018
·
IF 5.091
Chong-Bo Ma,Yan Du,Baoji Du,Hao Wang,Erkang Wang
An adsorbent that exhibits high affinity for inorganic mercury (Hg 2+ ) with a high removal efficiency of methylmercury (MeHg + ) has been developed. The adsorbent demonstrates a symbiotic relationship between its two components, molybdenum disulphide nanoflowers (MoS 2 NFs) and a poly (vinyl alcohol) (PVA) aerogel. Furthermore, we modified the distribution and loading of the MoS 2 NFs, which was possible due to the stable porous support, and investigated the biocompatibility of the aerogel-support adsorbent. The performance of the optimized material exhibited a distribution coefficient of 9.71 × 10 7 mL g −1 . In addition, the adsorbent was effective over a wide pH range and could efficiently purify both contaminated lake and sea water. The key motivation for using an aerogel support was to stabilise the MoS 2 NFs during purification of the water (resulting in improved performance compared to using freestanding MoS 2 NFs) and the ability to regenerate the used adsorbent. In addition, animal tests confirmed an extremely low toxicity of the adsorbent to fish, along with the excellent purification results.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
·
2018
·
IF 2.015
Dong-Xu Jia,Teng Wang,Zi-Jian Liu,Li-Qun Jin,Jia-Jia Li,Cheng-Jun Liao,De-Shui Chen,Yu-Guo Zheng
Glucose isomerase (GI) responsible for catalyzing the isomerization from d -glucose to d -fructose, was an important enzyme for producing high fructose corn syrup (HFCS). In a quest to prepare HFCS at elevated temperature and facilitate enzymatic recovery, an effective procedure for whole cell immobilization of refractory Thermus oshimai glucose isomerase (ToGI) onto Celite 545 using tris(hydroxymethyl)phosphine (THP) as crosslinker was established. The immobilized biocatalyst showed an activity of approximate 127.3 U/(g·immobilized product) via optimization in terms of cells loading, crosslinker concentration and crosslinking time. The pH optimum of the immobilized biocatalyst was displaced from pH 8.0 of native enzyme to neutral pH 7.0. Compared with conventional glutaraldehyde (GLU)-immobilized cells, it possessed the enhanced thermostability with 70.1% residual activity retaining after incubation at 90°C for 72 h. Moreover, the THP-immobilized biocatalyst exhibited superior operational stability, in which it retained 85.8% of initial activity after 15 batches of bioconversion at 85°C. This study paved a way for reducing catalysis cost for upscale preparation of HFCS with higher d -fructose concentration.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD BIOCHEMISTRY
·
2018
·Vol. 43
·期 3
·e12741
·
IF 1.552
Si-Qi Yang,Xiao-Yan Dai,Xiao-Yi Wei,Qing Zhu,Tao Zhou
Co-immobilization of pectinase and glucoamylase onto sodium alginate/graphene oxide beads was achieved by N , N′ -dicyclohexylcarbodiimide/ N -hydroxysuccinimide as activating agent. The co-immobilized pectinase-glucoamylase (I-PG) prepared under optimal conditions (pH 4.0, 40°C and 35 min) possessed pectinase activity of 1,227.5 ± 36.5U/g and glucoamylase activity of 1,027.2 ± 29.2U/g, with activity recovery of 73.8% and 85.2%, respectively. Both pectinase and glucoamylase in I-PG possessed wider pH tolerance and superior thermal stability to those of their free counterparts. Reusability studies indicated that both enzymes in I-PG retained over 60% of initial activity after six times of reuse. Conditions for the hydrolysis of the pumpkin–hawthorn compound juice by I-PG were optimized using orthogonal experiments. After treatment with I-PG, light transmittance, soluble solids, and reducing sugar content in the resulting juice increased significantly, whereas soluble protein and pectin content decreased appreciably. Therefore, the use of I-PG provided an effective and feasible method for improving quality of the pumpkin–hawthorn juice. Practical applications In order to overcome the drawbacks of using free pectinase and glucoamylase, an effective method for the co-immobilization of these two enzymes onto sodium alginate/graphene oxide beads was developed. The co-immobilized pectinase/glucoamylase developed in this study could be applied in the clarification of juice rich in pectin and starch.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Shucheng Liu,Xuan Zhang,Yue Ma,Xue Bai,Xueping Chen,Jinxin Liu,Jianming Pan
By immobilization of nano-sized adsorbents into a water-rich matrix to fabricate a hybrid hydrogel has raised considerable attention in aqueous phase molecular recognition, owing to the combination of inherent advantages of easy operation, good mechanical performance and abundant recognizing sites by a light-triggered chemical polymerization strategy. The boronic acid and vinyl-functionalized multi-walled carbon nanotubes (VMWCNTs-COOH-BA) were introduced to hydrogel matrix, and then as-prepared hybrid hydrogels (MBA-H-5.0) were applied for selective adsorption and separation of luteolin (LTL). Surface hydrophilicity, mechanical performance and specific surface area of the hybrid hydrogels were enhanced by the addition of functional VMWCNTs-COOH-BA. In static adsorption, MBA-H-5.0 showed a large LTL loading amount (35.91 mg g −1 at 308 K), fast capture kinetics (3.0 h), remarkable selectivity, and excellent recyclability (8.24% loss in adsorption amount after seven adsorption-desorption cycles). The abundant boron-containing functional groups such as boronic acid played a vital role on adsorption, and the copolymerization of VMWCNTs-COOH-BA and hydrogel matrix prevented from the leakage of nanostructured adsorbents. This study sheds new light on the design of high performance hybrid hydrogel by embedding functional adsorbents for selective separation and enrichment of natural flavonoids.
✓ 已验证
产品关联
DOI
高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2017
·Vol. 35
·期 7
·1098-1108
·
IF 1.852
Fengjin Qu,Xiaoyan Ma,Yuchen Hui,Fang Chen,Yan Gao
In this study, chemical reduced graphene-silver nanoparticles hybrid (AgNPs@CR-GO) with close-packed AgNPs structure was used as a conductive matrix to adsorb enzyme and facilitate the electron transfer between immobilized enzyme and electrode. A facile route to prepare AgNPs@CR-GO was designed involving in β -cyclodextrin ( β -CD) as reducing and stabilizing agent. The morphologies of AgNPs were regulated and controlled by various experimental factors. To fabricate the bioelectrode, AgNPs@CR-GO was modified on glassy carbon electrode followed by immobilization of glucose oxidase (GOx) or laccase. It was demonstrated by electrochemical testing that the electrode with close-packed AgNPs provided high GOx loading ( Γ =4.80 × 10 −10 mol•cm −2 ) and fast electron transfer rate ( k s =5.76 s −1 ). By employing GOx based-electrode as anode and laccase based-electrode as cathode, the assembled enzymatic biofuel cell exhibited a maximum power density of 77.437 μW•cm −2 and an open-circuit voltage of 0.705 V.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 6
·1-13
·
IF 5.3
Gao Lu,Zhang Ling,Yang Juanli,Ma Tong,Wang Bo,Yang Huan,Lin Feng,Xu Xuechao,Yang Zhen-quan
A broad host range phage-based nanozyme (Fe-MOF@SalmpYZU47) was prepared for colorimetric detection of multiple Salmonella enterica strains. The isolation of a broad host range phage (SalmpYZU47) capable of infecting multiple S. enterica strains was achieved. Then, it was directly immobilized onto the Fe-MOF to prepare Fe-MOF@SalmpYZU47, exhibiting peroxidase-like activity. The peroxidase-like activity can be specifically inhibited by multiple S. enterica strains, benefiting from the broad host range capture ability of Fe-MOF@SalmpYZU47. Based on it, a colorimetric detection approach was developed for S. enterica in the range from 1.0 × 10 2 to 1.0 × 10 8 CFU mL −1 , achieving a low limit of detection (LOD) of 11 CFU mL −1 . The Fe-MOF@SalmpYZU47 was utilized for detecting S. enterica in authentic food samples, achieving recoveries ranging from 91.88 to 105.34%. Hence, our proposed broad host range phage-based nanozyme exhibits significant potential for application in the colorimetric detection of pathogenic bacteria. Graphical Abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）