Foods
·
2022
·Vol. 11
·期 19
·3136
·
IF 5.561
Xiaohu Zhou,Chaohua Zhang,Liangzhong Zhao,Wenhong Cao,Chunxia Zhou,Xin Xie,YuLian Chen
Isoelectric solubilisation/co-precipitation (ISP) has been proven to be a better method than blending for preparing plant–animal dual-proteins, which can achieve synergies in the functional properties of heterologous proteins. This paper aims to investigate the effect of extraction pH on the functional properties of co-precipitated dual-protein. The basic composition, subunit composition, solubility, surface hydrophobicity, emulsification and gel properties of co-precipitated dual-protein (Co) prepared from pea and grass carp with pH (2.0, 3.0, 9.0, 10.0 and 11.0) were analysed in this study using ISP. The results showed that the functional properties of Co (Co9, Co10, Co11) prepared by alkali extraction were generally better than those prepared by acid extraction (Co2, Co3). Among them, Co10 has the highest vicilin/legumin α + β value and solubility, while having the lowest surface hydrophobicity, making its emulsification and gel properties superior to other extraction pH values. This study provides an important method reference for preparing plant-animal Co with exceptional functional properties.Keywords:pea;co-precipitated dual-protein;blended dual-protein;plant protein;grass carp;surface hydrophobicity;emulsifying properties;gel properties;mixed protein
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Xin Zhang,Ke Jiang,Huajun He,Dan Yue,Dian Zhao,Yuanjing Cui,Yu Yang,Guodong Qian
A fluorescent pH sensor based on post-synthetically modified nanoscale UiO-67-bpydc by encapsulating Eu 3+ cations in the pores was fabricated. The Eu 3+ incorporated sample (Eu 3+ @UiO-67-bpydc) shows excellent luminescence due to the sensitization and protection provided by the parented framework. Subsequently, Eu 3+ @UiO-67-bpydc was developed as a pH sensor. Eu 3+ @UiO-67-bpydc shows good chemical resistance to both acidity and alkalinity solutions with pH ranging from 1.06 to 10.99, which makes it potentially useful for wide-range pH sensor. In addition, cycling experiments have demonstrated the utilizability of the sensor. The MTT analysis shows that Eu 3+ @UiO-67-bpydc possesses favourable biocompatibility. Furthermore, fluorescence imaging of Eu 3+ @UiO-67-bpydc in PC12 cells was also performed to test its feasibility in vivo. Given its exceptional stability and pH-dependent fluorescent intensity, the fluorescent probe should be a promising candidate for application in fluorescent pH sensing in vitro and in vivo.
✓ 已验证
DOI
高影响因子
PHARMACEUTICAL RESEARCH
·
2020
·Vol. 37
·期 7
·1-13
·
IF 3.242
Chen Weiguo,Li Ji,Xing Yan,Wang Xiaowei,Zhang Hongyan,Xia Mingyu,Wang Dongkai
Purpose The complex physiological barriers impose extremely conflicting demands on systemic drug delivery, so both particle size and surface charge of the nanoplatforms become vital factors. As a carbon-based nanomaterial with excellent optical properties, carbon dots are not suitable for direct systemic transport in vivo , which limits their application in the field of biomedical imaging, especially in the areas of diagnosis and cancer treatment. Liposomes have been developed as universal nanocarriers for various drugs. In this study, we aimed to build a highly precise and penetrative drug delivery system (DDS) using carbon dots encapsulated by liposomes. Methods Carbon dots (CDs) were synthesized by the hydrothermal method using citric acid and ethylenediamine. Furthermore, simian virus 40 large T-antigen derived the nuclear targeting sequence (NLS) was bonded on the surface of CDs to obtain CDs-NLS. The antitumor drug doxorubicin was loaded onto the CDs-NLS through an acid-labile hydrazine bond to obtain DOX@CDs. Finally, DOX@CDs were encapsulated in aqueous centers of folate-coated and pH-sensitive liposomes, named pHSL-FA. Results In this paper, a nucleus-targeted nanocomposite (DOX@CDs), which bonds with the nuclear targeting sequence (NLS) and the anticancer drug doxorubicin (DOX), has physicochemical properties of particle size of about 3.8 nm, zeta potential of +31.8 mV and high quantum yield of 64.53%. The negatively charged folate-coated and pH-sensitive liposomes (pHSL-FA) are used as a carrier to reverse the surface charge of DOX@CDs. Compared to free DOX@CDs, pHSL-FA show higher tumor accumulation in 4 T1 tumor-bearing mice and further improve cytotoxicity to tumor cells. Conclusions This work proposes a unique nanomedical approach that enables the precise delivery of chemotherapy drugs and significantly reduces side effects, which is promising for clinical translation.
✓ 已验证
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2017
·
IF 3.887
Zili Gu,Minglu Chang,Yang Fan,Yanbin Shi,Guimei Lin
As current tumor chemotherapy faces many challenges, it is important to develop drug delivery systems with increased tumor-targeting ability, enhanced therapeutic effects and reduced side effects. In this study, a pH-sensitive liposome was constructed containing CHEMS-anchored PEG2000 for extended circulation and NGR peptide as the targeting moiety. The NGR-modified docetaxel-loaded pH-sensitive extended-circulation liposomes (DTX/NGR-PLL) prepared possess suitable physiochemical properties, including particle size of approximately 200 nm, drug encapsulation efficiency of approximately 70%, and pH-sensitive drug release properties. Experiments performed in vitro and in vivo on human fibrosarcoma cells (HT-1080) and human breast adenocarcinoma cells (MCF-7) verified the specific targeting ability and enhanced antitumor activity to HT-1080 cells. The results of intravenous administration demonstrated that NGR-modified liposomes can significantly and safely accumulate in tumor tissue in xenografted nude mice. In conclusion, the liposomes constructed hold promise as a safe and efficient drug delivery system for specific tumor treatment.
✓ 已验证
DOI
PubMed
高影响因子
BIOMATERIALS
·
2014
·
IF 8.312
Hui-zhen Jia,Jun-yi Zhu,Xu-li Wang,Han Cheng,Gang Chen,Yi-fang Zhao,Xuan Zeng,Jun Feng,Xian-zheng Zhang,Ren-xi Zhuo
Advanced drug delivery systems, which possess post-functionalization feasibility to achieve targetability and traceability, favorable pharmacokinetics with dynamic but controllable stability, and preferable tumor accumulation with prolonged drug residence in disease sites, represent ideal nanomedicine paradigm for tumor therapy. To address this challenge, here we reported a dynamic module-assembly strategy based on reversible boronic acid/1,3-diol bioorthogonality. As a prototype, metastable hybrid nanoself-assembly between hydrophobic hyperbranched diol-enriched polycarbonate (HP-OH) and hydrophilic linear PEG terminated with phenylboronic acid (mPEG-PBA) is demonstrated in vitro and in vivo . The nanoconstruction maintained excellent stability with little leakage of loaded drugs under the simulated physiological conditions . Such a stable nanostructure enabled the effective in vivo tumor accumulation in tumor site as revealed by NIR imaging technique . More importantly, this nanoconstruction presented a pH-labile destruction profile in response to acidic microenvironment and simultaneously the fast liberation of loaded drugs. Accordingly at the cellular level, the intracellular structural dissociation was also proved in terms of the strong acidity in late endosome/lysosome, thus favoring the prolonged retention of remaining drug-loaded HP-OH aggregates within tumor cells. Hence, our delicate design open up a dynamical module-assembly path to develop site and time dual-controlled nanotherapeutics for tumor chemotherapy, allowing enhanced tumor selectivity through prolonged retention of delivery system in tumor cells followed by a timely drug release pattern.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2022
·Vol. 46
·期 5
·e16547
·
IF 2.609
Kun Ma,Hui Zhang,Enjie Diao,Shiquan Qian,Peng Xie,Ruifeng Mao,Huwei Song,Liming Zhang
Ultrasound was used to degrade patulin in pH 3.5 of solution containing cysteine, and investigated the cysteine-enhanced effects on ultrasound degradation of patulin. The results showed that ultrasound alone did not efficiently degrade patulin (reduced by 4.96%–8.90%) without adding cysteine, and their synergistic roles were more favorable for patulin degradation (reduced by 17.82%–60.98%). The molar ratio between cysteine and patulin, ultrasonic power and time, and water cooling of sample during ultrasound treatment, all significantly affected the patulin degradation efficiency, and obtained 79.33% of degradation rate after 60 min of ultrasound treatment at 540 W of power. Both the logarithmic decay model and zero-kinetic model well simulated its degradation processes ( R 2 > 0.95). The radical reaction may be the main sonolysis mechanism of patulin in the presence of cysteine. Additionally, the patulin degradation efficiency induced by heating was far less than that by ultrasound under other same conditions. Novelty impact statement Cysteine enhanced the ultrasound degradation of patulin in highly acidic conditions. Degradation mechanism of patulin by ultrasound in the presence of cysteine was deduced. Sonolysis kinetics of patulin in the presence of cysteine were simulated.
✓ 已验证
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Yingying Zhang,Yanliang Zhao,Yingnan Wu,Bing Zhao,Liyan Wang,Bo Song
Lysosome as the typical acidic organelle involved in many biological processes, the dysfunction of its pH would alter many diseases. Small-molecule fluorescent probe has been considered as vital tool for monitoring pH fluctuation in living cells. Herein, a hemicyanine based ratiometric fluorescent probe was synthesized, namely, 2,3-trimethy-3-[2-(dimethyl-amino-4-yl) vinyl]-3 H -benzo[ e ]indole ( BiDL ), for rapidly detection pH under acidic conditions. BiDL exhibited ratio fluorescence emission ( F 534nm / F 622nm ) characteristic with p K a 4.25. BiDL showed good linearly response in pH 3.4–4.82, indicating that the probe can be used for quantitative detection pH. The probe also displayed large Stokes shift (112 nm/201 nm) under neutral and acidic conditions, respectively, which could effectively reduce the excitation interference. BiDL had excellent cell membrane permeability , good photo-stability and low toxicity in living cells. The dual-channel confocal fluorescent microscopic ratiometric imaging application of pH in HeLa cells and lysosome were achieved successfully, indicating that BiDL has good potential for investigating lysosome-relevant pathological and physiological processes.
✓ 已验证
DOI
PubMed
高影响因子
Journal of Biomedical Nanotechnology
·
2016
·Vol. 12
·期 8
·1688-1698
·
IF 3.929
Zhang Xueqiong,Zhang Hong,Yin Liangquan,Hu Rui,Qiu Tong,Yin Yihua,Xiong Xiong,Zheng Hua,Wang Qun
Nanoparticles, such as polymeric micelles, are currently regarded as effective drug delivery vehicles. In this study, a series of carboxymethyl chitosan/daunorubicin (CMCS/DNR) conjugates with macromolecular carriers of different molecular weights (MWs) were prepared and structurally
characterized by Fourier transform infrared spectroscopy (FT-IR) and 1H-NMR spectroscopy. The results indicated that the MWs of the carriers greatly influenced the drug loading capacity. DNR was conjugated to the polymer via an acid-sensitive hydrazone linkage susceptible to hydrolysis
at pH 5–6, thereby enabling intracellular DNR release. In aqueous media, the conjugates spontaneously formed nano-sized particles with core–shell structures (CMCS and DNR as the shell and core, respectively). Dynamic light scattering (DLS) and trans-mission electron microscopy
(TEM) indicated that the prepared nanoparticles were spherical in shape with diameters of 100–200 nm and had a slight negative surface charge. In addition, in vitro drug release studies demonstrated that the micelles were highly sensitive to mild acidic conditions (pH 5.0) but
remained reasonably stable at pH 6.5 and pH 7.4. Importantly, cell counting kit-8 (CCK-8) assays demonstrated that DNR-conjugated nanoparticles exerted a cytotoxic effect on HeLa cells, with the IC50 being approximately 2 times higher than that of free-DNR. Furthermore, confocal
laser scanning microscopy (CLSM) revealed that the CMCS-hyd-DNR nanosystem could efficiently deliver and release DNR in the nuclei of cancer cells. Taken together, the developed CMCS based pH-sensitive nanosystem may be a potential drug delivery vehicle for cancer therapy.
✓ 已验证
DOI
PubMed
高影响因子
BIOMATERIALS
·
2014
·
IF 8.312
Qi-Ming Wang,Zhonggao Gao,Shan Liu,Bo Fan,Lin Kang,Wei Huang,Mingji Jin
The bioactive polymer poly( l -glutamic acid) n - b -poly( d , l -lactic acid) m was synthesized and used to form doxorubicin-loaded hybrid polymeric micelles to treat melanoma. These polymers exhibited pH-responsive changes in conformation, which controlled the diverse functionalities of the micelles. During circulation, poly( l -glutamic acid) n - b -poly( d , l -lactic acid) m protected Tat peptides on the micelles from proteolysis . Under tumor-acidic conditions, polymers with shorter poly( l -glutamic acid) blocks underwent a conformational change to form channels that accelerated the release of doxorubicin . The conformational change also exposed the Tat peptides to tumor cells, thereby promoting cellular internalization of the micelles. Enhanced cellular uptake of the micelles induced significant apoptosis of A375 melanoma cells in tumor-acidic conditions. In vivo studies demonstrated that the micelles with shorter poly( l -glutamic acid) blocks could effectively accumulate in tumor tissues, suppress tumor growth and help maintain the body weight of tumor-bearing mice. However, micelles with longer poly( l -glutamic acid) blocks did not undergo a conformational change under acidic conditions and performed poorly in both in vitro and in vivo evaluations. Our work provides a strategy for applying bioactive polymers to the rational construction of pH-responsive delivery systems for solid tumors and lends insight into possible conformational effects on the bioactivity of drug carriers.
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2020
·
IF 0
Min Mu,Yuelong Wang,Shasha Zhao,Xiaoling Li,Rangrang Fan,Lan Mei,Min Wu,Bingwen Zou,Na Zhao,Bo Han,Gang Guo
Chemotherapy works against tumors by inducing cell apoptosis; however, evasion of apoptosis is recognized to result in resistance to anticancer therapy. Ferroptosis is an iron-dependent cell death pathway that differs from apoptosis in morphological, biochemical, and genetic levels. Combined ferroptosis and apoptosis may shed light on strategies for cancer treatment. Therefore, we have designed a nanoparticle (NP) that can simultaneously cause tumor cell apoptosis and ferroptosis. This NP is composed of epigallocatechin gallate (EGCG) and Fe3+ through a simple and green process and can be used to deliver doxorubicin hydrochloride (DOX) and iron ions to the tumor site at the same time. DOX/Fe3+/EGCG (DF) NPs display a great resolubility and long-term storage stability, and efficient DOX and Fe3+ release is realized after cellular internalization under the high level of glutathione and acidic nature in tumor. EGCG is likely to chemically reduce the released Fe3+ to Fe2+. The generated Fe3+/Fe2+ converts intracellular H2O2 to hydroxyl radicals (•OH) via the Fenton reaction. In addition, the generated •OH subsequently induces lethal ferroptosis to improve DOX-induced apoptosis. In vitro and in vivo investigations indicate that a great therapeutic effect was achieved, suggesting that the formation of the DF NP delivery system is a promising strategy to fight against tumors by an apoptosis and ferroptosis combination modality.
✓ 已验证
DOI
PubMed
BIOMACROMOLECULES
·
2020
·
IF 6.092
Zexuan Ding,Guhuan Liu,Jinming Hu
Visualization of intracellular transport pathways is crucial to investigate the internalization mechanism and understand the intracellular behavior of nanomaterials. Herein, we rationalized the design of micellar nanoparticles (NPs) for ratiometric fluorescent mapping of intracellular pH and glutathione (GSH), two essential parameters for maintaining normal cellular functions. Specifically, pH-sensitive naphthalimide-based probe (NPI) and pH-inert rhodamine B (RhB) were covalently labeled to double hydrophilic block copolymers (DHBCs) using the thiolactone chemistry, enabling the covalent attachment of NPI and RhB to one molecule with a redox-responsive disulfide linkage. The dually labeled DHBCs exhibited blue/orange dual emissions in acidic pH, which was further converted into green/orange dual emissions in neutral pH because of the deprotonation of NPI moieties and the sole green emission in the presence of GSH at neutral pH because of the decreased Förster resonance energy transfer efficiency between an NPI donor and an RhB acceptor as a result of GSH-mediated cleavage of disulfide bonds. These remarkable ratiometric fluorescence changes allowed for not only the simultaneous mapping of the intracellular pH and GSH but also the intracellular transport pathways of internalized NPs.
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Yuanyuan Zhang,Xin Du,Xuechun Wang,Xuanzhi Li,Liuqing He,Jiahui Li,Xiaohu Wang
Microfluidic chip monitors 1.9 μL urine pH from 4.0 to 9.0. • Fiber-collimated optics and ML resist urine pigment, turbidity, hemolysis. • GBDT-LRGB model is optimized and verified with over 2000 groups of data. • 92.5% samples show pH residuals < 0.2, within clinical 0.5 deviation. • Adaptable for organ-on-a-chip, single-cell culture, and IVD devices. The demand for stable and accurate continuous pH monitoring of trace liquids in microfluidic chips keeps rising, yet existing methods fail to balance five core indicators: stability, accuracy, measurement range, anti-interference and low cost. This work constructs a microfluidic pH detection system via optimized optical layout, 3D microfluidic structure and machine learning. Fiber coupling and collimating lenses homogenize light inside the micro-cuvette. Combined with 3D mixing channels and matched indicators, GBDT and logarithmic RGB features realize stable pH detection (4.0–9.0) for liquid volumes as low as 1.9 μL. Models are trained with 1890 measurements obtained from 6 replicate measurements of 265 clinical urine samples and 50 simulated samples. In random testing, 92.5% of samples have residuals below 0.2 pH units, and all samples fell within the total allowable error (TEa) of 0.5 pH units for the dipstick method. The maximum SD obtained from the measurements was 0.066 in the 48 h continuous stability test. Under the experimentally tested conditions, the system tolerated urine yellow background up to G4, turbidity up to 200 NTU, mild hematuria/hemolysis up to R3, and variations in ionic strength down to 10 −6 . This method demonstrates potential for certain clinical urine pH testing applications and could be applicable to trace pH measurement in selected life science scenarios, including cell culture, organoid culture, continuous-flow microfluidics, and POCT. Download: Download high-res image (174KB) Download: Download full-size image The pH monitoring system for trace urine analysis in microfluidic chips
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）