JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Bo Luo,Yanbing Wu,Mengfan Cao,Chendan Liu,Heran Xiong,Yuqing Guo,Miao Wang,Lu Yu,Ruxin Han,Mengxing Liu
In the ongoing search for more potent succinate dehydrogenase inhibitors (SDHIs) for agricultural applications, 30 pyrazole-4-carboxamide derivatives bearing a thioether group were designed and synthesized via bioisosteric replacement and scaffold-hopping based on the structure of two SDHIs lead compounds in our previous work. The antifungal efficacy of these target compounds was evaluated against five major plant pathogenic fungi, and the results indicated that the majority of the synthesized compounds exhibited strong antifungal activities, particularly against Rhizoctonia solani and Sclerotinia sclerotiorum. Among these, compound 8e emerged as the most promising candidate, displaying exceptional antifungal potency against R. solani (EC50 = 0.012 μg/mL) and S. sclerotiorum (EC50 = 0.123 μg/mL), significantly superior to boscalid (EC50 = 0.464 μg/mL and 0.159 μg/mL, respectively) and comparable to fluxapyroxad (EC50 = 0.036 μg/mL and 0.104 μg/mL). Additionally, detached leaf assays revealed that compound 8e effectively inhibited the growth of R. solani on rice leaves and S. sclerotiorum on oilseed rape leaves, achieving significant protective efficacies of 89.1% and 93.9%, respectively, at 100 μg/mL, which were superior to boscalid (68.8% and 90.5%) and comparable to fluxapyroxad (86.4% and 98.6%), indicating a promising potential for crop protection in the field, particularly for rice and oilseed rape. Furthermore, SDH enzymatic inhibition assays showed that compound 8e exhibited potent SDH inhibition (IC50 = 1.30 μM), which was more effective than boscalid (IC50 = 1.53 μM) and close to fluxapyroxad (IC50 = 0.35 μM), confirming its role as an effective SDHI. SEM analysis showed that compound 8e caused significant structural disruption and morphological damage to the hyphae of R. solani, further supporting its antifungal activity. The results of molecular docking and DFT and ESP calculations demonstrated that compound 8e had a similar antifungal mechanism to fluxapyroxad. These findings suggested that compound 8e held great promise as a potential SDHI candidate for protection of crops such as rice and oilseed rape, warranting further investigation.
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JOURNAL OF BIOTECHNOLOGY
·
2024
·
IF 4.1
Xiuqi Zhou,Jiayan Du,Jinyuan Zhu,Xueqing Pang,Xinjian Yin,Pingping Zhou
The utilization of genetically modified microbial cells for rosmarinic acid (RA) production is gaining increased attention as a cost-effective and sustainable approach. However, the substrate promiscuity of 4-coumarate: CoA ligase and RA synthase has been considered as a critical factor for low RA yields. In this study, we rationally engineered the substrate preference of 4-coumarate: CoA ligase (OPc4CL2) from Petroselinum crispum , resulting in a significant enhancement in RA production. Particularly, the introduction of the Y240C mutation led to a remarkable 176 % increase in RA yield. Subsequent enzymatic analysis of OPc4CL2 variants revealed diminished activity towards p -coumaric acid, resulting in insufficient time for the transformation of p -coumaric acid to 4-coumaroyl CoA to generate byproduct. Furthermore, to minimize the formation of undesired byproducts, the overexpression of 4-hydroxyphenylacetate 3-monooxygenase (OHpaB) and NADPH-flavin oxidoreductase (HpaC) was carried out to facilitate the conversion of p -coumaric acid to caffeic acid and 4-hydroxyphenyllactate to salvianic acid A, thus achieving a significant increase in RA yield of up to 329.9 mg/L (16.5 mg/g yield on glucose) in shake-flask cultivation. Finally, the engineered strain YRA113–24BHM achieved a notable RA production of 3.6 g/L (about 20.2 mg/g yield on glucose) by fed-batch fermentation. This study serves as a foundation for the sustainable biosynthesis of RA and other caffeic acid derivatives.
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Cancer Management and Research
·
2024
·
IF 2.5
Yantao Yu,Chenkai Zhang,Qiannan Sun,Shantanu Baral,Jianyue Ding,Fanyu Zhao,Qing Yao,Shuyang Gao,Bin Liu,Daorong Wang
Background Gastric cancer (GC) is a highly phenotypically heterogeneous disease and is caused by a combination of factors. Retinol binding protein 4 (RBP4) is a member of a family of lipid transport proteins that are involved in the transport of substances between cells and play a crucial role in a variety of cancers. However, the expression and role of RBP4 in GC remain unknown.Methods In this study, we explored the expression, prognostic significance, immune microenvironment, drug responsiveness and function of associated signaling pathways of RBP4 in GC using web-based bioinformatics tools. Immunohistochemistry and real-time quantitative PCR were utilized to analyze the tissue and cell expression levels of RBP4. CCK-8, colony formation, EDU incorporation, wound healing and transwell assays were applied to demonstrate the effect of RBP4 on GC cell function. Flow cytometric detection of apoptosis after RBP4 knockdown. Nude mice xenograft model elucidates the role of RBP4 for GC in vivo. Related proteins of the RAS signaling pathway were analyzed by employing Western blot assays.Results RBP4 is highly expressed in GC. RBP4 is closely associated with patient survival and sensitivity to a wide range of antitumor agents. Knockdown of RBP4 promoted apoptosis and inhibited cell proliferation, invasion and migration. RBP4 promotes GC tumorigenesis in vivo. Finally, RBP4 modulates the RAS/RAF/ERK axis.Conclusion RBP4 may promote gastric carcinogenesis and development through the RAS/RAF/ERK axis and is expected to be a novel target for GC treatment.
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Journal of Environmental Sciences
·
2013
·
IF 1.773
Kashif Naeem,Feng Ouyang
The photocatalytic degradation of phenol and 4-chlorophenol (4-CP) in aqueous suspensions with the use of titanium dioxide (TiO 2 ) under UV irradiation was examined. The effects of different supporting materials mixed physically with TiO 2 were studied to achieve maximum degradation efficiency. Among the three supports, namely activated carbon (AC), silica (SiO 2 ) and zeolite (ZSM-5), all exhibited paramount efficiency for degradation of phenol and 4-CP and was better than TiO 2 alone. The optimum concentration was found to be 50 mg for all supporting materials. The efficiency order of the three supports was as follows: AC > ZSM-5 > SiO 2 , respectively. Whilst, the degradation of phenol and 4-CP was improved from 70.6% to 87.6% and 80.6% to 89.7%, respectively, within 120 min photocatalysis in the presence of optimal amount of AC. The degradation was also comparatively enhanced in the presence of cheaper rice husk and the activity was closed to ZSM-5 and lower than AC.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2025
·
IF 9
Tunga Kuhana Arsene,Miaomiao Tian,Yatao Zhang
The increasing demands of modern industrialization underscore the need for efficient separation and purification technologies. Multifunctional nanofiltration (NF) membranes are gaining prominence as a means to lower energy consumption, enhance efficiency, and increase process flexibility. This study explores the development of thermally stable NF membranes through interfacial polymerization (IP), employing organophosphorus bis(4-aminophenyl) phenylphosphonate (BAPP) and trimesoyl chloride (TMC) as monomers in the aqueous and organic phase, respectively. The optimized BAPP-4 membrane (containing 0.1550 w/v% BAPP), which presented a nodular buried structure, exhibited exceptional water permeability (9.23 L m −2 h −1 bar −1 ) and high Na 2 SO 4 rejection (98.52 %). Furthermore, the membrane exhibited enhanced antibacterial properties, achieving 98.00 % Escherichia coli removal, attributed to hydrogen bonding and electrostatic interactions involving amide and phosphorus groups. The BAPP-4 membrane also showed rapid flux recovery in antifouling tests, primarily due to its surface hydrophilicity. Notably, the utilization of BAPP increased the rigidity of the cross-linked polyamide network, leading to improved thermal stability. This research presents a facile and effective approach for developing high-performance and multifunctional NF membranes.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Qian Wu,Xinping He,Chenyi Cui,Chenchen Jiao,Baojin Qi,Jinjia Wei
Mixed matrix membranes (MMMs) incorporating metal–organic frameworks (MOFs) hold significant promise for gas separation applications. However, the inherent disparity between the organic polymer matrix and the inorganic fillers frequently leads to interfacial incompatibility issues. In this study, we adopted an in-situ interfacial crosslinking strategy to fabricate MMMs incorporating NH 2 -Zn 2 (Bim) 4 nanosheets that simultaneously act as nanofillers and reactive crosslinkers. Specifically, during the thermal imidization reaction process, the amino groups of NH 2 -Zn 2 (Bim) 4 not only establish hydrogen bonding interactions with the carbonyl groups of polyamic acid (PAA) (the precursor of polyimide), but undergo dehydration reactions with carboxyl groups, leading to the formation of stable amide linkages. Meanwhile, the aromatic structure of the BI units, a key monomer of polyimide, closely resembles that of the ligands of NH 2 -Zn 2 (Bim) 4 , which can establish π-π stacking interactions, further improving the interfacial compatibility between the polymer matrix and MOF fillers. Among the prepared samples, MMMs incorporating 33.3 wt% NH 2 -Zn 2 (Bim) 4 nanosheets exhibit the maximum H 2 permeability of 680.5 Barrer, and its H 2 /N 2 and H 2 /CO 2 selectivities were 18.7 and 13.3, respectively, which were close to or even exceeded the Robeson upper bound. The proposed in-situ interfacial engineering based on chemical crosslinking provides a novel and effective route for fabricating high-performance MMMs with enhanced interfacial compatibility, demonstrating great potential for hydrogen separation applications.
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CERAMICS INTERNATIONAL
·
2025
·Vol. 51
·期 25
·44694-44701
·
IF 5.6
Honglei Yuan,Huizhong Ma,Gaoliang Wang,Huanxia Jia,Liwei Wang
This study systematically investigates the structural and luminescent characteristics of LaSc 3 (BO 3 ) 4 :x %Cr 3+ and La (1-y) Gd y Sc 3 (BO 3 ) 4 :1.0 %Cr 3+ phosphors. X-ray diffraction confirms the formation of a pure hunter-type structure for all samples, with Cr 3+ occupying Sc 3+ sites and Gd 3+ substituting La 3+ , inducing lattice contraction. Luminescent analysis reveals that LaSc 3 (BO 3 ) 4 :Cr 3+ exhibits an emission at 980 nm under 430 nm excitation, attributed to the 4 T 2 → 4 A 2 transition of Cr 3+ in a weak crystal field. The substitution of Gd 3+ enhances photoluminescence intensity by 2.15-fold, and blue-shifts emission to 938 nm because of increased crystal field splitting and structural rigidity. The broadened full width at half maximum is also formed due to the enhanced degree of disorder in GdSc 3 (BO 3 ) 4 . Thermal stability studies show that GdSc 3 (BO 3 ) 4 :Cr 3+ retains 49 % intensity at 540 K, outperforming LaSc 3 (BO 3 ) 4 :Cr 3+ (41 %), supported by higher activation energy (ΔE = 0.338 eV vs. 0.296 eV). The improved thermal resistance is linked to lattice rigidity and reduced electron-phonon coupling. NIR-pc-LEDs fabricated with GdSc 3 (BO 3 ) 4 :Cr 3+ demonstrate superior radiant flux (68.7 mW at 200 mA) compared to La-based devices. These results highlight the critical role of host lattice engineering in optimizing Cr 3+ luminescence for high-power NIR applications.
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Materials Science & Engineering C-Materials for Biological Applications
·
2018
·
IF 5.08
Cheng-bin Gong,Yue-hong Yang,Mei-jun Chen,Lan-tao Liu,Song Liu,Yu-bo Wei,Qian Tang
The trans to cis isomerization of the azobenzene chromophore in most azobenzene-based photoresponsive molecularly imprinted polymers (MIPs) is initiated by UV irradiation. This limits the application of these materials in cases where UV light toxicity is an issue, such as in biological systems, food monitoring, and drug delivery. Herein we report a tetra- ortho -methyl substituted azobenzene, (4-[(4-methacryloyloxy)-2,6-dimethyl phenylazo]-3,5-dimethyl benzenesulfonic acid (MADPADSA). The photoswitching of MADPADSA could be induced by visible-light irradiation (550 nm for trans to cis and 475 nm for cis to trans ) in 4-hydroxyethylpiperazineethanesulfonic acid (HEPES) buffer-ethanol (4:1, v / v ) at pH 7.0, however, the photoisomerization was slow. With the use of MADPADSA as a functional monomer, NaYF 4 :Yb 3+ ,Er 3+ as a substrate, 4-ethylphenol (4-EP) as a template, a novel photoresponsive surface molecularly imprinted polymer NaYF 4 :Yb 3+ ,Er 3+ @MIP was obtained. The NaYF 4 :Yb 3+ ,Er 3+ @MIP displayed rapid visible-light-induced photoswitching. The NaYF 4 :Yb 3+ ,Er 3+ substrate could efficiently increase the trans to cis isomerization rate of the photoresponsive MIP on its surface, which was faster than that of the corresponding azobenzene monomer MADPADSA. Possible reasons for this effect were investigated by fluorescence spectroscopy. NaYF 4 :Yb 3+ ,Er 3+ @MIP displayed good specificity toward 4-EP with a specific binding constant ( K d ) of 3.67 × 10 −6 mol L −1 and an apparent maximum adsorption capacity ( Q max ) of 10.73 μmol g −1 , respectively. NaYF 4 :Yb 3+ ,Er 3+ @MIP was applied to determine the concentration of 4-EP in red wine with good efficiency and a limit of detection lower than the value that could cause an unpleasant off-flavor.
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OPTICAL MATERIALS
·
2019
·
IF 2.687
Yanling Wang,Yachao Du,Lingli Meng,Yueshu Li,Zhi Li,Qingwen Tian
Kesterite Cu 2 ZnSn(S,Se) 4 (CZTSSe) has attracted much attention as an ideal absorber material for thin film solar cells. Solution deposition methods provide a crucial approach for lowering the fabricating price of CZTSSe based photovoltaic devices . Here, a low-cost and robust ethanol/thioglycolic acid/1-butylamine ternary solutions method was developed to fabricate the CZTSSe solar cells. Characterization was performed using XRD , Raman, and C–V, which showed that the material fabricated by this method expressed high-phase purity, suitable carrier concentration, and large depletion width, and those features are vital to high efficiency photovoltaic devices . Hence, based on this high quality CZTSSe absorbed layer, photovoltaic device with a power conversion efficiency (PCE) as high as 9.71% was achieved. The PCE can be further enhanced through passivating with alkali metal and optimizing the device fabrication conditions, making this method much promising for lowering the manufacturing cost of kesterite solar cells. Additionally, this approach is full of application prospect for other burgeoning photovoltaic absorber materials which could dramatically reduce the cost of solar electricity.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2019
·
IF 4.175
Yu Zhang,Xuejie Zhang,Haoran Zhang,Zhan-Chao Wu,Yingliang Liu,Li Ma,Xiaojun Wang,Wei-Ren Liu,Bingfu Lei
A series green emission phosphors Sr 3 Lu 2 (BO 3 ) 4 :Ce 3+ ,Tb 3+ (denoted as SLBO:Ce 3+ ,Tb 3+ ) with broad absorption band matching well with UV LED chip were synthesized by the solid–state reaction method. The phase and structure were analyzed with XRD , EDX , FT-IR measurements. The energy transfer between Ce 3+ and Tb 3+ in SLBO was proved by spectra and decay curves detailedly. The emission colors vary from blue to green by adjusting the ratio of Ce 3+ /Tb 3+ doped concentration. With temperature increasing, SLBO:Ce 3+ ,Tb 3+ phosphor shows well thermal stability that emission intensity retains 70% of its initial at 25 °C even temperature rising to 150 °C. The internal and external quantum efficiency of SLBO:3%Ce 3+ ,8%Tb 3+ are 77.5% and 34.8% under 340 nm excitation, respectively. Combining the as-synthesized SLBO:Ce 3+ ,Tb 3+ and red emission phosphor CaAlSiN 3 :Eu 2+ coated on a 365 nm UV chip, a warm white LEDs device with CRI of 83 was achieved. All the results indicate that the SLBO:Ce 3+ ,Tb 3+ phosphor has potential application in UV pumped white LEDs.
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Journal of Analytical Methods in Chemistry
·
2015
·Vol. 2015
·期 1
·387207
·
IF 0.792
Ying Liang,Yingming Pan,Qing Guo,Hongzhi Hu,Chancui Wu,Qian Zhang
A novel fluorescent reagent for determination of ammonium, 4-methoxyphthalaldehyde (MOPA), was successfully synthesized in this study. Under alkaline conditions, MOPA could reacted with ammonium rapidly at room temperature, producing fluorescent substance which had maximum excitation at 370 nm and emission wavelength at 454 nm. Based on this, a novel fluorescence analysis method was established for the determination of trace ammonium in natural water. Experimental parameters including reagent concentration, pH, reaction equilibrium time, and metal ions masking agent were optimized. The results showed that the optimized MOPA concentration was 0.12 g/L, pH was in the range of 11.2–12.0, and sulfite concentration was 0.051 g/L, respectively. Metal ions masking agent had no obvious effect on the fluorescence signal. With the reaction time of 15 minutes, linear range of this method was between 0.025 and 0.300 μ mol/L, and the method detecting limit was 0.0058 μ mol/L. The matrix recovery of the proposed method was in the range of 93.6–108.1%. Compared with the OPA method, this method was much more sensitive and rapid without the interference of background peak and would be more suitable for developing a portable fluorescence detection system.
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CANADIAN JOURNAL OF CHEMICAL ENGINEERING
·
2025
·
IF 1.9
Yu-cheng Jiang,Bao-guang Zhu,Xiang-li Long
The oxidation of 1-(4-chlorophenyl)-pyrazolidin-3-one to 1-(4-chlorophenyl)-1H- pyrazol-3-ol by air can be accelerated by the Co(II)@AC catalyst prepared by loading Co(II) on activated carbon with wet impregnation method. ZnCl 2 solution has been used to treat activated carbon to ameliorate the catalytic ability of Co(II)@AC catalyst. The experiments indicate that the optimal catalyst is made by immersing activated carbon in 0.7 mol/L ZnCl 2 solution at 50°C for 6 h with a liquid/solid ratio of 30/1 (mL/g) followed being calcined at 700°C for 5 h at a heating rate of 5°C/min under N 2 protection. The 1-(4-chlorophenyl)-1H-pyrazol-3-ol concentration catalyzed by the catalyst loaded on the best carbon reaches 0.01953 kg/L, which is 27.56% higher than that catalyzed by the catalyst loaded on the original carbon. The characterization results manifest that the modification with ZnCl 2 enriches the porous structure and increases the acidic groups on the surface of activated carbon. The conversion of 1-(4-chlorophenyl)-pyrazolidin-3-one is mainly determined by the physical structure of the activated carbon while the selectivity to 1-(4-chlorophenyl)-1H- pyrazol-3-ol is largely affected by the chemical characteristics of the activated carbon.
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