Cell Metabolism
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2025
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IF 30.9
Tiezhu Shi,Jialiang Shao,Yufeng Ding,Hong Tang,Xiangyin Tan,Sisi Zhou,Shaoqing Yu,Xiang Wang,Guanzhen Yu,Ninghan Feng,Xiongjun Wang
Metabolic adaptations involved in tumor metastasis and immune evasion merit investigation. Here, using in vivo metabolic CRISPR/Cas9 knockout screening, we identified xylulokinase (XYLB) as a tumor suppressor that impairs lung colonialization by producing xylulose 5-phosphate (Xu5P), which promotes CD8 + T cell cytotoxicity. Mechanistically, CD8 + T cells express relatively high levels of solute carrier family 35 member E2 (SLC35E2), a homolog of the plant Xu5P transporter, to facilitate Xu5P uptake and subsequently intensify the pentose phosphate pathway and glycolysis for energy/redox balance. Furthermore, we revealed that Xu5P potentiates CD8 + T cell response by promoting Xu5P-responsive progenitor-like SLC35E2 + CD8 + exhausted T cells via tet methylcytosine dioxygenase 3 (TET3)-mediated DNA demethylation of the Tcf7 promoter. Clinically, elevated XYLB or blood Xu5P correlates with enhanced CD8 + T cell efficacy and reduced metastasis. In murine models, Xu5P supplementation or adopting Xu5P-rich diets synergizes with anti-PD-1 therapy to enhance antitumor immunity. These findings offer insights into the potentiality of dietary interventions for metastatic cancer.
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Journal of Environmental Chemical Engineering
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2021
·
IF 5.909
Xu Duan,Jian Yang,Guang Hu,Chen Yang,Yangfan Chen,Qingcai Liu,Shan Ren,Jiangling Li
In this work, based on a type of energy band engineering strategy as calcination , TiO 2 /ZSM-5 hybrids for photodegradation of methyl orange (MO) in water under UV light have been developed via a facile solid state diffusion method. Effects of varied calcination temperatures on TiO 2 /ZSM-5 hybrids have been systematically characterized including XRD , FT-IR, SEM, TEM , EDX mapping, BET, PL , EIS , UV–vis DRS, active species trapping experiments and Mott-Schottky curves. It was found that the calcination temperature influenced photocatalytic activity , typically on physicochemical properties of materials and adjustment for the energy gap and band position of TiO 2 . The XRD analysis revealed that hybridization of ZSM-5 kept the crystal phase of TiO 2 as anatase unchanged with the high-rising calcined temperature. The optimum sample calcined at 450 °C named as Z-T (450) presented nearly 99% degradation rate for MO, owing to optimal uniform distribution, highest specific surface area and best charge separation/transfer efficiency. The FT-IR comparison result that the functional groups and peak strength of the used catalyst were alike as before, showed the stability of photocatalyst . Adoption of calcination strategy does provide not only a clean, low-cost and highly efficient path to approach the complete degradation of azo dyes , but also a chance for photocatalytic technology to fulfill the implementation in industrial applications.
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NEW JOURNAL OF CHEMISTRY
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2014
·Vol. 38
·期 8
·3673-3677
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IF 3.159
Xi Juan Zhao,Cheng Zhi Huang
A one step synthesis of water-soluble luminescent copper nanoclusters (CuNCs) reduced and protected by histidine is reported. When excited at 350 nm, the as-synthesized CuNCs exhibit luminescence centered at 456 nm with a quantum yield of 1.6%, which is different from that of histidine alone under the same experimental conditions. Upon addition of guanosine 5′-triphosphate (GTP), the emission of CuNCs is gradually quenched. While other nucleoside triphosphates including ATP, CTP and UTP as well as some inorganic anions such as P2O74−, PO43−, and CH3COO− have no such quenching effect, which indicates that the nucleobase instead of the phosphate anion plays an important role in the emission quenching of CuNCs. The most efficient quenching of the CuNCs induced by GTP can be ascribed to the most reducing ability of guanine among the other nucleobases. This interesting experimental result demonstrates that the as-synthesized CuNCs can be applied to sensing of GTP.
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CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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2016
·Vol. 44
·期 8
·1286-1290
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IF 0.566
Li-Hua DENG,Jin-Bo DAI,Zhen-Lin XU,Jin-Yi YANG,Hong WANG,Zhi-Li XIAO,Hong-Tao LEI,Yuan-Ming SUN,Yu-Dong SHEN
To detect furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) in fish sample, a Eu 3+ labeling time-resolved fluoroimmunoassay (TRFIA) was developed. The experimental conditions were optimized as follows: the AMOZA-OVA concentration was 0.25 μg mL −1 ; the antibody was diluted by 50000 folds, and the competitive reaction time was 50 min. Under the optimal conditions, the method showed a detection limit of 0.01 ng mL −1 , an IC 50 of 0.26 ng mL −1 and a linear range (IC 20 −IC 80 ) of 0.025−2.83 ng mL −1 . The spiked recoveries of AMOZ in fish at three spiked levels ranged from 78.0% to 86.0%, and the relative standard deviations were less than 15%. Good correlation between ic-TRFIA and high performance liquid chromatography-tandem mass spectrometry was obtained for spiked food samples. The proposed ic-TRFIA method was suitable for the determination of AMOZ residue in food samples.
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CHINESE JOURNAL OF CHEMISTRY
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2014
·Vol. 33
·期 1
·125-130
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IF 1.04
Ting Zhou,Hao Yu,Mingqiang Liu,Ying-Wei Yang
Carboxylated pillar[5]arene (CP5) macrocycles were modified onto the surface of reduced graphene oxide (RGO) via covalent bonds to form new pillarene-functionalized RGO nanosheets, i.e. , RGO-CP5. The obtained RGO-CP5 nanosheets capable of molecular recognition exhibited much better water-dispersibility and enhanced fluorescence-quenching property as compared with native RGO. This new sensing platform holds great potentials in the sensing and detection of analytes, such as organic dye molecules and pesticides, in aqueous solutions.
研究领域:
分析化学
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纳米材料科学
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传感器技术
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环境监测
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分子识别
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MICROPOROUS AND MESOPOROUS MATERIALS
·
2024
·
IF 4.8
Yuxin Li,Juanping Zhou,Hu Wen,Haitao Yin,Shunyu Han,Nanzhe Jiang
Clay is a rich aluminosilicate resource with unique advantages as a raw material for zeolites production. However, synthesizing nanosized single-crystalline zeolites through a simple approach using clay remains a significant challenge. In this paper, acid-washed montmorillonite (MSR) was used to prepare nano-H-ZSM-5 single crystals with high crystallinity, uniform grain size and permeable straight pores under the induction of a small amount of organic template tetrapropylammonium hydroxide (TPAOH). Through temperature-staged treatment, the nucleation and growth process of zeolite were decoupled, ultimately achieving precise control over the size of the nano-single crystals. Mechanistic study showed that the low-temperature aging process produces smaller structural units which probably derived from the geopolymer topology of the clay, providing more active sites for zeolite nucleation, leading to the rapid crystallization during the high-temperature process. The porous structure promotes material diffusion, inhibits aromatic cycling and reduces carbon formation in the pores. The large specific surface area and nanoscale multilayer pore structure delay carbon accumulation and increase carbon holding capacity. These properties make nano-ZSM-5 single crystals an excellent choice for high olefin selectivity and long catalytic life in the methanol-to-olefin reaction. This synthetic route provides a general strategy for preparing nanosized zeolite single crystals from clay.
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JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
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2026
·
IF 6.7
Hong Tian,Ziwen Zhao,Zhangjun Huang,Yanshan Yin,Song Ren,Shan Cheng
The presence of nitrogen-containing compounds (NCCs) in bio-oil severely limits its downstream utilization as a clean fuel. To address this issue, a catalytic strategy combining hierarchical pore construction and bimetallic active-site modification was developed for the deep denitrogenation and upgrading of biomass pyrolysis oil. Bamboo (BB) and wheat straw (WS) were selected as representative forestry and agricultural residues, respectively, and Ni-Co bimetal-modified HZSM-5 catalysts were prepared. Appropriate alkali treatment significantly improved the pore structure of HZSM-5, increasing its specific surface area from 383.9 to 409.12 m 2 /g, while Ni and Co introduction effectively regulated the distribution of acidic sites. As a result, the modified catalysts enhanced the pyrolysis of BB and WS and improved the quality of the resulting bio-oils. Over 6Ni0.5Co-HR5, aromatic selectivity reached 51.06% for BB and 50.66% for WS. GC/MS analysis showed that the catalyst promoted the transformation of nitrogen species from kinetically unstable chain-like compounds into more stable cyclic structures, facilitating their further removal. Among the catalysts, 6Ni0.5Co-HR5 exhibited the best denitrogenation performance, reducing the relative total nitrogen content in BB- and WS-derived bio-oils to 0.89% and 0.67%, respectively, corresponding to decreases of 96.34% and 93.02% compared with non-catalytic pyrolysis. This superior perf ormance was attributed to the synergistic effect of the hierarchical pore structure and Ni-Co bimetallic sites. The results demonstrate good feedstock adaptability of the catalyst and provide an effective route for producing low-nitrogen, aromatic-rich bio-oil.
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LANGMUIR
·
2026
·
IF 4.4
Siyao Zhang,Si Liu,Linling Yu,Yan Sun
Aminotransferases (ATAs), essential enzymes for the synthesis of chiral amine drugs, play a crucial role in the synthesis of sitagliptin. The catalytic process of ATAs relies on the effective synergistic action of the cofactor pyridoxal 5′-phosphate (PLP). However, practical applications face challenges such as the poor stability of ATAs and the inefficient recovery of the expensive cofactor PLP. To address these issues, this study proposes a high-efficiency and stable catalytic nanoreactor, ATA&PLP@PAH-BioHOF-1, fabricated by polyelectrolyte poly(allylamine hydrochloride) (PAH)-mediated coencapsulation of both ATA and PLP in a hydrogen-bonded organic framework, BioHOF-1, for the synthesis of sitagliptin. This immobilization system overcomes the limitations of traditional single immobilization methods that were unable to simultaneously maintain enzyme activity and sustainably reuse the cofactor. The system exhibits excellent loading capacity and catalytic performance, achieving a sitagliptin yield of over 95% within 4 h. Additionally, the system demonstrates outstanding stability, retaining 78.3% of its initial sitagliptin yield (95%) after 18 consecutive catalytic cycles (4 h each), with a total PLP turnover number of 175. This study has thus provided an efficient nanoreactor system for the sustainable production of sitagliptin with in situ PLP recycling.
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ACS Catalysis
·
2026
·Vol. 16
·期 15
·14434-14449
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IF 13.6
He Tianli,Chen Haoxi,Li Senyuan,Jin Yanshuo,Yu Xiang,Huang Wenyu,Xie Fangyan,Chen Jian,Pan Dingjie,Wang Nan,Meng Hui,Chen Shaowei
Oxidation of 5-hydroxymethylfurfural (HMF) has been proposed as a promising alternative to the oxygen evolution reaction to enhance hydrogen production in alkaline media. However, the mechanistic understanding of the HMF oxidation reaction (HMFOR) has remained limited. In this study, CoSe 2 -Cu 2−x Se heterostructure composites are synthesized via a two-step hydrothermal method using cobalt(II) acetate, cupric acetate, and selenium powders as the starting materials, and the sample with a 0.4 mmol initial feed of cupric acetate stands out as the best HMFOR catalyst among the series, achieving a current density of 10 mA cm −2 at a potential of +1.265 V vs RHE and a 97% Faradaic efficiency for the production of 2,5-furandicarboxylic acid at +1.5 V vs RHE. The catalytic activity is attributed to the lattice oxygen within the amorphous hydroxyl oxide layer formed via surface reconstruction of the catalyst, which can directly and indirectly participate in HMFOR during the indirect oxidation of CoOOH/CoO 2 . Such a lattice oxygen-mediated indirect oxidation mechanism of CoOOH/CoO 2 is supported by in situ spectroscopic characterizations, 18 O-isotope labeling experiments, and density functional theory calculations. The findings of this work provide new insights into the electro-oxidation of small organic molecules, an important process for advancing electrochemical energy technologies.
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CHEMICAL ENGINEERING SCIENCE
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2026
·
IF 5.1
Jiaqi Li,Dongfeng He,Ruize Zhang,Binbin Tan,Jiang Wang,Jing Wang,Junrong Chen,Hui Wang,Wenlei Wang
5-Hydroxymethylfurfural (HMF) serves as a key platform molecule for green photocatalytic oxidation to produce high-value-added chemicals. Addressing the critical need for efficient photocatalytic conversion of HMF to 2,5-furan dicarboxylic acid (FDCA) under mild conditions, this study proposes and constructs a synergistic ternary composite photocatalytic system. By incorporating porous organic polymers (POPs) into oxygen-vacancy-rich MnO 2-x /Cu 2 O, the MnO 2-x @POPs@Cu 2 O composite photocatalyst was synthesized. This composite catalyst demonstrated remarkable photocatalytic performance under ambient conditions, achieving 71.8% conversion of HMF and 18.5% yield of FDCA. Results indicate that POPs not only selectively enrich reactants by forming dynamic imine bonds with HMF via its surface (–NH 2 ) functional groups, but also significantly enhance interfacial charge separation and transport through a locally embedded electric field generated by its highly negatively charged triazine framework, thereby accelerating the Mn-Cu bimetallic redox cycle. Furthermore, electron paramagnetic resonance (EPR) and density functional theory (DFT) calculations confirmed that introducing POPs effectively reduces O 2 adsorption energy, promotes molecular oxygen activation, and enhances reactive oxygen species generation. This study provides a novel strategy for designing low-cost, high-efficiency multicomponent photocatalysts for biomass refining.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Jiaxin Gao,Yanjie Wang,Peipei Fu,Xiaojun Yan,Denghui Zhu
Peroxiredoxins (Prxs) are crucial antioxidant enzymes that protect against oxidative damage and participate in immune responses. However, the immunological function of Prx5 in bivalves remains unclear. In this study, the peroxiredoxin gene McPrx5 was cloned from Mytilus coruscus , and its molecular characteristics and biological functions were analyzed. A 1491 bp cDNA sequence was obtained, containing a 573 bp open reading frame (ORF) that encodes a 190-amino-acid protein (20.29 kDa) with features of an atypical 2-Cys Prx. Tissue distribution analysis revealed that McPrx5 was highly expressed in the foot, gill, and digestive gland. Immune challenges with LPS, poly I:C, and Perkinsus olseni induced dynamic changes in McPrx5 expression. Subcellular localization analysis of the McPrx5-GFP fusion protein showed that McPrx5 was exclusively localized in the cytoplasm. Recombinant McPrx5 enhanced the tolerance of Escherichia coli BL21 to H₂O₂, confirming its antioxidant activity. Importantly, in vivo experiments demonstrated that McPrx5 negatively regulates the expression of key molecules in the TLR4 / MyD88 / NF-κB signaling pathway, as well as FOXO transcription factors, suggesting its important role in linking redox homeostasis with immune regulation. These findings clarify the biological functions of McPrx5 in M. coruscus and provide a new basis for understanding the coordinated regulatory mechanisms linking antioxidant defense to innate immunity in bivalves.
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ACS Catalysis
·
2026
·
IF 13.6
Mengqi Ding,Xinhong Zhao,Yanru Li,Xuefeng Long,Hongwei Li,Dong Ji,Guixian Li
Iron-containing zeolites have shown great potential in catalyzing phenol hydroxylation with H2O2 to produce dihydroxybenzenes (catechol and hydroquinone). However, their industrial application has been hindered by low selectivity and the complexity of active-site identification. This study addresses these challenges by synthesizing a series of Fe-ZSM-5 catalysts with iron contents up to 4 wt % via an ionic liquid-assisted interzeolite transformation route. Through systematic regulation of transformation temperature and calcination atmosphere, combined with an acid treatment step, a catalytically inert benchmark sample (T180-Air) was strategically prepared and compared with three active variants (T200-Air, T180-N2, T200-N2) to achieve the isolation and identification of active sites. Employing a “differential comparison strategy”, combined with DR UV–vis, Raman, Mössbauer, XANES/EXAFS, EPR, H2-TPR, in situ FT-IR characterizations, and DFT calculations, we systematically excluded the possibility of binuclear iron species, small FexOy clusters, and distorted tetrahedral iron as primary active sites. The evidence consistently points to extra-framework octahedrally coordinated mononuclear iron as the key active species, which effectively activates both phenol and H2O2 to generate reactive intermediates. Moreover, the high-selectivity formation of dihydroxybenzenes is driven by a synergistic interaction between these mononuclear octahedral iron sites and neighboring strong acid centers. The optimal catalyst (T200-Air) achieved a phenol conversion of 46.7% and a dihydroxybenzene selectivity of 95.6% under dark conditions, with its dihydroxybenzene selectivity surpassing that of state-of-the-art Fe-based catalysts at comparable phenol conversion levels. This work elucidates the critical importance of the synergistic design between the active iron species and the local acidic microenvironment, providing clear experimental evidence and structure-performance guidelines for the rational design of high-performance iron-zeolite catalysts for selective phenol hydroxylation reactions.
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