Journal of Taibah University for Science
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2024
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IF 2.8
Lingyu Wang,Ruolan Li,Meiyan Li,Jiayi Sun,Jia Liu,Min Xie,Yunhui Chen,Songqi Tang
This study aimed to investigate the inhibitory effect of Zuojin Pill (ZJP) on KYSE 150 cells and xenograft tumours of the digestive tract cancer – esophageal cancer (EC) and its underlying mechanism. Initially, in vivo research demonstrated that oral administration of ZJP effectively inhibited the growth of EC tumours, and in vitro studies revealed that ZJP suppressed the proliferation, migration, and invasion of cells. Then, network pharmacology prediction identified the core target, active ingredient, and involvement of the PI3K-Akt signalling pathway in ZJP treatment of EC. Cell studies validated that ZJP affected the expression of core targets, and its active ingredients inhibited the proliferation of cells. Additionally, ZJP was shown to induce cell apoptosis, decrease mitochondrial membrane potential, and increase the level of intracellular oxidative stress. Further experimental verifications suggested that the inhibitory effects of ZJP on EC might be attributed to the modulation of PI3 K/Akt signalling pathways.
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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2025
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IF 4
Xin-Yv Li,Yu-Jie Liu,Xian-Hua Meng,Chu Gong,Jun-Li Yang,Yin Qiang,Jun Wang,Xiu-Dan Hou
Anthracnose is a highly contagious and destructive plant disease caused by Colletotrichum gloeosporioides . The objective of this study was to investigate the antifungal activity and mechanism of ginsenoside Rk1 ( GRk1 ) against C. gloeosporioides. In vitro experiments demonstrated that GRk1 exhibited substantial antifungal activity, with EC 50 values of 64.58 mg L −1 , surpassing the plant-derived pesticide d -limonene . Additionally, GRk1 could effectively suppress the dissemination of anthracnose spots in postharvest fruit. By observing the microstructure of C. gloeosporioides , it was observed that the mycelium structure underwent notable changes, and autophagosomes engulfed the organelles in the experimental groups. Fluorescent staining revealed specific signals in mycelial autophagosomes, and Western Blot analysis confirmed the upregulation of the autophagy-related protein Atg8. These findings indicate that GRk1 may activate the autophagy process by promoting Atg8 expression. Proteomic analysis revealed that the expression of Sec20 and Mpv17 proteins was significantly downregulated, whose dysregulated expression is associated with the crosstalk between SNAREs and peroxisomes during vesicle trafficking and metabolic processes. Assessment of catalase (CAT) activity, hydrogen peroxide (H₂O₂) content, and superoxide anion (O₂ − ) levels further demonstrated that GRk1 could disrupt intracellular reactive oxygen species (ROS) homeostasis in mycelia. Moreover, the introduction of the ROS scavenger N -acetylcysteine (NAC) markedly inhibited autophagy, indicating that GRk1 promotes autophagy by inducing oxidative stress. Collectively, GRk1 inhibits pathogens via the ROS-autophagy axis. These findings suggested that GRk1 holds potential as a promising alternative to fungicides for the biological control of anthracnose.
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ADVANCED MATERIALS
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2025
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IF 26.8
Heng Yang,Xiaoke Jia,Ting Wang,Jiangge Li,Wei Geng,Mohsen Adeli,Tian Ma,Yang Gao,Chong Cheng,Weifeng Zhao
Chronic refractory wounds present substantial clinical difficulties, owing to their complex wound microenvironments featuring bacterial colonization, sustained inflammation, and deficient angiogenesis. Existing treatment options often fall short of concurrently overcoming these interconnected obstacles, underscoring the demand for integrated therapeutic platforms that combine antimicrobial, anti-inflammatory, and pro-angiogenic properties. Drawing inspiration from natural peroxisomes, a lattice-reconstructed Ru-clusters are designed on FeOOH-based self-adaptive artificial peroxisome with programmed reactive oxygen species (ROS) regulation for infectious and inflammatory chronic wounds. Within this architecture, electron-rich Ru clusters and hole-rich FeOOH domains establish an efficient electron-transfer network. Density functional theory calculations demonstrate that this distinctive electronic structure lowers reaction energy barriers, enabling pH-switchable ROS-catalytic behaviors. Under acidic wound pH, LR-RuC@FeOOH catalyzes ROS generation to disrupt bacterial metabolism and eliminate infections, while under neutral conditions, it efficiently scavenges ROS to alleviate oxidative stress and support tissue repair, enabling a logically sequenced therapeutic progression from infection control to inflammation resolution. In vivo experiments using diabetic foot ulcers confirmed that LR-RuC@FeOOH significantly enhanced bacterial clearance, attenuated inflammatory responses, stimulated neovascularization, and accelerated wound closure. These results position LR-RuC@FeOOH as an artificial peroxisome with stage-specific therapies and promising translational potential for the treatment of refractory diabetic wounds and other infection-related pathologies.
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ADVANCED FUNCTIONAL MATERIALS
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2025
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IF 19
Xinting Feng,Zhiwen Luo,Wei Zhang,Renwen Wan,Yisheng Chen,Fangqi Li,Yanwei He,Zhiheng Lin,James Hoipo Hui,João Conde,Shiyi Chen,Zhijie Zhao,Xianwen Wang
Volumetric muscle loss (VML) is a severe condition in which the loss of skeletal muscle surpasses the body's intrinsic repair capabilities, leading to irreversible functional deficits and potential disability, with persistent inflammation and impaired myogenic differentiation. To address these challenges, a novel zinc-dihydromyricetin (Zn-DHM) nanozyme with superoxide dismutase (SOD)-like activity is developed, designed to neutralize excessive reactive oxygen species (ROS) and restore oxidative balance. Zn-DHM mitigates oxidative stress and promotes polarization of macrophages from the proinflammatory M1 phenotype to the anti-inflammatory M2 phenotype, thereby reducing chronic inflammation and creating a conducive environment for muscle repair. Further, Zn-DHM significantly enhances the myogenic differentiation of C 2 C 12 cells, accelerating wound healing processes. These studies confirm the biosafety and low toxicity of Zn-DHM. As per a murine tibialis anterior VML model, Zn-DHM effectively suppresses inflammation and markedly improves skeletal muscle repair outcomes. Single-cell RNA sequencing reveals that Zn-DHM treatment increases the expression of M2 macrophage markers and enhances the proliferation and differentiation capacity of muscle stem cells (MuSCs). In addition, intercellular communication analysis reveals interactions between MuSCs and macrophages in the Zn-DHM treatment group, suggesting that these interactions may drive tissue regeneration through the activation of the GAS and Notch signaling pathways.
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WATER RESEARCH
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2025
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IF 12.4
Hongzhi Liu,Zi Wang,Hesong Wang,Jiaxuan Yang,Tianyi Wang,Zihan Liu,Han Zhang,Heng Liang,Langming Bai
To alleviate the global water crisis, membrane technology is widely employed in cooling water reuse processes. However, excessive microbial growth induced by industrial waste heat leads to severe membrane biofouling. This study constructed a customized armor coating composed of tannic acid (TA), copper (Cu), and polyethylenimine (PEI) on a commercial membrane surface via a minimalist approach. The coating achieved superior eradication efficiency (>95%) against planktonic bacteria through Cu²⁺-catalyzed reactive oxygen species (ROS) generation. The average adhesion force between the membrane and bacterial coaggregates decreased significantly from 11.5 nN to 7.58 nN following modification. Furthermore, extracellular polymeric substance (EPS) components exhibited significantly reduced adhesion propensity on the membrane surface. This is attributed to the robust hydration layer provided by the metal-polyphenol network (MPN), which substantially elevated the membrane's surface energy barrier and adhesion resistance. Long-term synthetic wastewater testing demonstrates that a robust dual-action anti-fouling mechanism ensures highly efficient biofilm suppression capability, effectively extending the lifespan of nanofiltration membranes. This work expands the application of MPN-based materials in the membrane field, providing a minimalist yet highly efficient and innovative paradigm for developing antibacterial membrane surfaces.
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BIOMATERIALS
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2026
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IF 13.6
Hongyu Lin,Qingyan Peng,Ying Lin,Xiao Yang,Fengmei Zhang,Fangyin Dai,Shixiong Yi
Drug-resistant bacteria infecting wounds and impeding their healing have become a pressing clinical challenge. Antimicrobial photodynamic therapy (aPDT) does not pose a risk of bacterial resistance, but its application is limited due to the short lifespan, restricted diffusion distance, and non-selective biological toxicity of reactive oxygen species (ROS). Herein, we developed a visible-light-responsive organic nanoplatform (iTPyPXs/SCM) that improves ROS utilization for antimicrobial therapy while limiting excessive intracellular ROS. The platform consists of cationized photodynamic organic nanoparticles (iTPyPXs) integrated with a silk fibroin-based nanofibrous membrane (SCM) via electrospray deposition. Cationization enhanced the local donor–acceptor (D-A) structure and electrostatic adhesion of iTPyPXs, improving visible-light-induced ROS generation at the bacteria–material interface. Meanwhile, SCM immobilized iTPyPXs and released silk fibroin to reduce excessive intracellular ROS, thereby supporting an M2-associated reparative immunomodulatory tendency. In a rat model, iTPyPXs/SCM-aPDT eradicated infection and accelerated wound closure, while promoting inflammation resolution, angiogenesis, and collagen deposition. Transcriptomic analysis further confirmed that the treatment promoted regenerative healing. Collectively, this work provides a safer and more effective biomaterial strategy for photodynamic therapy in infected wound healing.
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SENSORS AND ACTUATORS B-CHEMICAL
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2026
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IF 8.3
Yongfei Huang,Li Zhang,Zhefeng Fan
Photodynamic therapy (PDT) holds great promise for tumor treatment, but its clinical translation is greatly hindered by the drawbacks of conventional photosensitizers (PSs), such as insufficient reactive oxygen species (ROS) generation, exclusive type‑II photoreaction, poor subcellular targeting, and compromised efficacy in hypoxic tumors. Herein, we rationally designed and synthesized a mitochondria‑targeted near‑infrared (NIR) photosensitizer Mito‑IsTp with a classic D-π-A architecture, using isophorone as the electron acceptor, methoxy‑modified triphenylamine as the electron donor, benzene as the π‑bridge, and triphenylphosphonium as the mitochondrial‑targeting group. Mito‑IsTp displays a maximum emission peak at 770 nm within the NIR‑I window, together with satisfactory photostability and solvent‑dependent optical performance. Upon 660 nm NIR light irradiation, Mito‑IsTp can simultaneously produce type‑I (•OH, O 2 -• ) and type‑II ( 1 O 2 ) ROS with much higher efficiency than commercial Rose Bengal and Chlorin e6 (Ce6). Confocal imaging verifies its excellent mitochondrial targeting ability in HeLa cells with a Pearson correlation coefficient of 0.97. In vitro experiments reveal negligible dark toxicity and efficient phototoxicity to induce cancer cell apoptosis through ROS-mediated mitochondrial damage. In vivo studies on HeLa tumor‑bearing nude mice further show that Mito‑IsTp ‑mediated PDT significantly suppresses tumor growth without obvious systemic toxicity or organ injury, confirmed by body weight recording, H&E staining, blood routine tests, and hemocompatibility assessment. This work presents a high‑performance NIR PS with dual‑pathway ROS generation and precise mitochondrial targeting, establishing a feasible paradigm for hypoxia‑resistant and mitochondria‑targeted tumor PDT.
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PROGRESS IN ORGANIC COATINGS
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2026
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IF 7.9
Xin Yu,Qinghe Gao,Guoxuan Li,Zhigang Lei,Chao Wang,Li Zhang,Huijuan Qian,Mingliang Zhu,Jin Huang,Conying Lu,Zhichao Zhang
Microbiologically influenced corrosion (MIC) induced by sulfate-reducing bacteria (SRB) severely threatens the service of metal materials, and traditional purely passive barrier coatings fail to withstand the continuous attack of biofilms. Herein, a ternary composite material (LDH-ZIF@CaO₂) composed of CoAl-LDH, ZIF-67, and CaO 2 was synthesized and incorporated into epoxy resin to construct an anti-corrosion coating that integrates active antibacterial and anti-corrosion properties. The study reveals that this composite possesses typical microenvironment responsiveness: in the acidic environment generated by microorganism metabolism, the Co ions released from the system can catalyze H₂O₂ (produced by CaO₂ hydrolysis) to undergo a Fenton-like reaction, generating highly oxidizing hydroxyl radicals (•OH) and singlet oxygen ( 1 O₂). This achieves highly efficient eradication of SRB and biofilm detachment. Electrochemical impedance spectroscopy (EIS) tests indicate that when the CaO₂ loading is 200 mg, the coating exhibits the optimal anti-corrosion performance, maintaining high compactness and high impedance characteristics even after 30 d of immersion, the impedance value is 10 8 Ω cm 2 , which is 2 orders of magnitude higher than that of epoxy resin systems. Furthermore, this paper thoroughly elucidates that the excessive addition of CaO₂ (400 mg) leads to filler agglomeration, internal gas pressure, and oxidative degradation of the resin matrix caused by excessive free radicals, which conversely accelerates coating failure. This study provides a new theoretical basis and strategy for designing microenvironment-responsive, anti-MIC coatings.
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Advanced Healthcare Materials
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2026
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IF 11
Yanlin Li,Xiaotian Jiang,Jiale Li,Hanwen Gu,Qi Li,Jiaxin Li,Yunqing Ji,Xiang Min,Bin Chen,Qingjia Xu,Junhao Lin
Osteoarthritis (OA), a prevalent degenerative joint disorder characterized by articular cartilage deterioration, represents a major global health challenge. Recent studies highlight the pivotal role of reactive oxygen species (ROS)-mediated ferroptosis in OA pathogenesis. Here, we report a microenvironment-adaptive hydrogel system based on 2D vanadium nitride (V 2 N) MXene nanoenzyme (hereafter denoted as V 2 N MXenzyme@Gel). This system features a dual-crosslinked network that optimally balances between mechanical robustness and flexibility. Crucially, the V 2 N MXenzyme establishes a self-sustaining “capture-conversion-recycling” catalytic cycle, mimicking both superoxide dismutase (SOD) and catalase (CAT) activities. The cycle not only neutralizes detrimental ROS into water (H 2 O) and oxygen (O 2 ) but also generates nitric oxide (NO), collectively alleviating oxidative damage. Mechanistically, V 2 N MXenzyme@Gel effectively scavenges ROS, attenuates oxidative stress, and suppresses ferroptosis by regulating the Hspa5/GPX4 axis, while concurrently restoring the metabolic homeostasis of chondrocyte extracellular matrix (ECM). Meanwhile, intra-articular administration of V 2 N MXenzyme@Gel significantly attenuates cartilage degradation and inflammation levels in OA rats. Collectively, our work presents a V 2 N MXenzyme@Gel with dual capabilities for ROS elimination and ferroptosis blockade, demonstrating its efficacy in arresting OA progression and proposing a novel precision strategy for managing chronic joint diseases.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2026
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IF 8.7
Yating Sun,Qi Miao,Guanxin Zhao,Shengqi Wang,Min Liu,Xiuli Zhao,Jiulong Zhang
Diabetic wound (DW) is characterized by chronic inflammation and bacterial infection, making its healing a continuing clinical challenge. Herein, a living bacteria-loaded oxidized dextran hydrogel is successfully fabricated to accelerate wound healing via regulating inflammatory factors. Lactobacillus bulgaricus (LB), as a live microorganism, is encapsulated in the hydrogel via microcapsule technology with gelatin as a substance to reduce secondary infections and ensure the survival of LB. Gelatin microcapsules and oxidized dextran were used to prepare a pH/ROS responsive hydrogel (OPM@GLB) through a Schiff base reaction. Furthermore, mangiferin was also incorporated into the hydrogel by a dynamic borate ester bond to support desirable nutrition for LB. In vitro and in vivo results showed that the OPM@GLB hydrogel exhibited significant antibacterial and anti-inflammatory properties and promoted angiogenesis and collagen deposition to accelerate diabetic wound healing. This study may offer new insights for treating diabetic wound in the clinic.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2026
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IF 5.9
Bin Liu,Shiqiao Rui,Donglin Bian,Jiahui Wang,Shuaipeng Feng,Zhu Liu,Yuanqi Yang,Wanqing Gong,Long Wan,Junyan Zhang,Qinfu Zhao
Carbon dots with dual enzyme-like activities of SOD and CAT-like were synthesized. • An orally administered precursor solution underwent ROS-responsive gelation at sites of colonic inflammation. • Inverting M1/M2 macrophage imbalance and recruiting Treg cells to drive immune responses. • Restoring intestinal integrity and rebalancing the gut microbiota. Inflammatory bowel disease (IBD) involves a complex inflammatory environment marked by excessive oxidative stress, impaired barrier function, and microbial imbalance. These interconnected pathologies are difficult to address with conventional single-target therapies. To tackle these challenges, this study developed an intelligently responsive oral hydrogel delivery system based on sulfur-doped ginger carbon dots (SGCDs). SGCDs, synthesized via a hydrothermal method, not only exhibited remarkable antioxidant capacity but also demonstrated multiple enzyme-like activities, including SOD-like and CAT-like, along with stable fluorescence imaging properties. Simultaneously, thiolated hyaluronic acid (HS 1:6 ), exhibiting ROS-responsive gelation properties, was synthesized via chemical modification and employed as the gel matrix. The oral precursor system (HS 1:6 @SGCDs), constructed by loading SGCDs into the HS solution, underwent ROS-triggered in situ gelation upon reaching the colonic inflammatory region. This transformation formed a physical barrier, prolonged retention time, and enabled controlled release of SGCDs. This system significantly alleviated pathological symptoms through multiple mechanisms, including efficient scavenging of excessive ROS, modulation of the immune microenvironment, amelioration of intestinal barrier dysfunction, and amelioration of dysbiosis with a trend toward recovery of a more balanced microbial community. Through disease-directed and source-based innovation, the system combined targeted material design with multifunctional therapeutic capabilities. Thus, HS 1:6 @SGCDs provided an innovative and comprehensive solution for the treatment of IBD.
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LANGMUIR
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2025
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IF 3.9
Mi Liu,Weidan Zhao,Denghao Li,Dingyu Cui,Jiali Liu,Qingao Chen,Yan Zhou,Li Jiang,Huaibin Zhou
Infected wound healing remains a significant clinical challenge due to bacterial resistance and excessive inflammation. Herein, a multifunctional hydrogel dressing (Nb2C-Arg/Gel) is developed that integrates two-dimensional Nb2C nanosheets (photothermal agent) and arginine [nitric oxide (NO) donor] into a calcium ion cross-linked sodium alginate matrix. The hydrogel has a relatively high swelling capacity and good ability to scavenge reactive oxygen species (ROS), which are beneficial for absorbing wound exudate and reducing the inflammatory response of wounds. Under irradiation with a low-power NIR-II laser (1064 nm, 1 W cm–2), Nb2C-Arg/Gel achieves rapid photothermal heating (48.6 °C in 1 min), while thermally enhances arginine decomposition-triggered on-demand NO release. This synergistic mechanism provides superior antimicrobial efficacy against Staphylococcus aureus (97.65%) and methicillin-resistant S. aureus (90.05%). In vivo studies on MRSA-infected murine wounds demonstrate that the residual area on day 10 was 2.9%, which accelerated healing compared to that of the control group (17.8%), accompanied by reduced inflammation and enhanced angiogenesis. The excellent biocompatibility of hydrogel is validated by low hemolysis (<5%) and promoted fibroblast proliferation (125% cell viability). Combining with photothermal, NO-mediated antibacterial action, and ROS scavenging to alleviate oxidative stress, this antibiotic-free hydrogel dressing has great potential for clinical application for the treatment of refractory-infected wounds.
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