ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2022
·Vol. 414
·期 8
·2757-2766
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IF 4.478
Zhang Yanyan,Li Linze,Zhang Hao,Shang Junjian,Li Can,Naqvi Syed Muhammad Zaigham Abbas,Birech Zephania,Hu Jiandong
Abscisic acid (ABA), as the most common plant hormone in the growth of wheat, can greatly affect the yield when its levels deviate from normal. Therefore, highly sensitive and selective detection of this hormone is greatly needed. In this work, we developed an aptamer sensor based on surface-enhanced Raman spectroscopy (SERS) and applied it for the high sensitivity detection of ABA. Biotin-modified ABA aptamer complement chains were modified on ferrosoferric oxide magnetic nanoparticles (Fe 3 O 4 MNPs) and acted as capture probes, and sulfhydryl aptamer (SH-Apt)-modified silver-coated gold nanospheres (Au@Ag NPs) were used as signal probes. Through the recognition of the ABA aptamer and its complementary chains, an aptamer sensor based on SERS was constructed. As SERS internal standard molecules of 4-mercaptobenzoic acid (4-MBA) were encapsulated between the gold core and silver shell of the signal probes; the constructed aptamer sensor generated a strong SERS signal of 4-MBA after magnetic separation. When there were ABA molecules in the detection system, with the preferential binding of ABA aptamer and ABA molecule, the signal probes were released from the capture probes, after magnetic separation, leading to a linear decrease in SERS intensity of 4-MBA. Thus, the detection response was linear over a logarithmic concentration range, with an ultra-low detection limit of 0.67 fM. In addition, the practical use of this assay method was demonstrated in ABA detection from fresh wheat leaves, with a relative error (RE) of 5.43–8.94% when compared with results from enzyme-linked immunosorbent assay (ELISA). The low RE value proves that the aptamer sensor will be a promising method for ABA detection. Graphical abstract
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JOURNAL OF HEADACHE AND PAIN
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2022
·Vol. 23
·期 1
·1-14
·
IF 8.588
Pan Qi,Wang Yunfeng,Tian Ruimin,Wen Qianwen,Qin Guangcheng,Zhang Dunke,Chen Lixue,Zhang Yixin,Zhou Jiying
Background Central sensitization is an important pathophysiological mechanism of chronic migraine (CM), and microglia activation in trigeminocervical complex (TCC) contributes to the development of central sensitization. Emerging evidence implicates that blocking sphingosine-1-phosphate receptor 1 (S1PR1) can relieve the development of chronic pain and inhibit the activation of microglia. However, it is unclear whether S1PR1 is involved in the central sensitization of CM. Therefore, the purpose of this study is to explore the role of S1PR1 and its downstream signal transducers and activators of transcription 3 (STAT3) signaling pathway in the CM, mainly in inflammation.Methods Chronic intermittent intraperitoneal injection of nitroglycerin (NTG) established a mouse model of CM. First, we observed the changes and subcellular localization of S1PR1 in the trigeminocervical complex (TCC). Then, W146, a S1PR1 antagonist; SEW2871, a S1PR1 agonist; AG490, a STAT3 inhibitor were applied by intraperitoneal injection to investigate the related molecular mechanism. The changes in the number of microglia and the expression of calcitonin gene-related peptide (CGRP) and c-fos in the TCC site were explored by immunofluorescence. In addition, we studied the effect of S1PR1 inhibitors on STAT3 in lipopolysaccharide-treated BV-2 microglia.Results Our results showed that the expression of S1PR1 was increased after NTG injection and S1PR1 was colocalized with in neurons and glial cells in the TCC. The S1PR1 antagonist W146 alleviated NTG-induced hyperalgesia and suppressed the upregulation of CGRP, c-fos and pSTAT3 in the TCC. Importantly, blocking S1PR1 reduced activation of microglia. In addition, we found that inhibiting STAT3 signal also attenuated NTG-induced basal mechanical and thermal hyperalgesia.Conclusion sOur results indicate that inhibiting S1PR1 signal could alleviate central sensitization and inhibit microglia activity caused by chronic NTG administration via STAT3 signal pathway, which provide a new clue for the clinical treatment of CM.
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JOURNAL OF APPLIED PHYCOLOGY
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2022
·Vol. 34
·期 6
·2755-2767
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IF 3.404
Huo Shuhao,Wang Huiying,Chen Jing,Hu Xinjuan,Zan Xinyi,Zhang Cunsheng,Qian Jingya,Zhu Feifei,Ma Haile,Elshobary Mostafa
The total polysaccharide content of the sugar-rich filamentous microalga Tribonema minus (Xanthophyceae, Tribonematales) under 2% CO 2 was up to 50%. The optimal conditions for the extraction of polysaccharide by hot water extraction-ethanol deposition were as follows: A maximum polysaccharide yield of 27.25% was obtained under the optimized conditions of extraction temperature 80°C, extraction time of 2 h, pH 9, and liquid to raw material 0.5:1 mL mg −1 . The crude polysaccharides were mainly acidic polysaccharides containing sulfate groups and uronic acid, and the contents of which were 14.57% and 42.21 mg g −1 , respectively. The crude polysaccharide was mainly composed of rhamnose, arabinose, xylose, mannose, glucose, and galactose with a molar ratio of 0.31:0.26:0.40:0.42:1.00:0.47. In addition, the polysaccharide had a potential antioxidant activity in vitro. The scavenging effect and reducing power of polysaccharides increased with the increase of polysaccharide concentration. The scavenging effect was 56.11%, 75.6%, and 61.89% for 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH), superoxide, and hydroxyl radicals at a concentration of 2 mg L −1 , respectively. Hence, T. minus polysaccharides could be a source of natural antioxidants that might be used in foods or pharmaceuticals.
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Nature Communications
·
2022
·Vol. 13
·期 1
·1-8
·
IF 17.694
Su Qiang,Chen Shuming
Up-conversion electroluminescence, in which the energy of a emitted photon is higher than that of the excitation electron, is observed in quantum-dot light-emitting diodes. Here, we study its mechanism by investigating the effect of thermal energy on the charge injection dynamic. Based on the results of temperature-dependent electroluminescence and theoretical analysis, we reveal that at sub-bandgap voltage, holes can be successfully injected into quantum-dots via thermal-assisted thermionic-emission mechanism, thereby enabling the sub-bandgap turn-on and up-conversion electroluminescence of the devices. Further theoretical deduction and experimental results confirm that thermal-assisted hole-injection is the universal mechanism responsible for the up-conversion electroluminescence. This work uncovers the charge injection process and unlocks the sub-bandgap turn-on mechanism, which paves the road for the development of up-conversion devices with power conversion efficiency over 100%.
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CELLULOSE
·
2022
·Vol. 29
·期 3
·1393-1403
·
IF 6.123
Liu Siyuan,Shi Zhenxu,Wang Xuhong,Gong Yanbin,Li Xijun,Jia Xin,Gan Lin,Huang Jin
Assembling cellulose nanocrystals (CNCs) can induce solid-state photoluminescence based on Stokes scattering. Such photoluminescent materials are free of photo-quenching and show great potential in optical applications, whereas poor flexibility of assembled CNC arrays limits their applications. Here, a binary co-assembly of 1D nanoparticles and long-chain polymers was explored to produce the uniaxial CNC arrays into transparent poly(vinyl alcohol) (PVA) membranes, which enhanced the mechanical properties, especially ductility. Besides, the CNC assembly was controlled by adjusting the mass ratio between CNCs and PVA. The result indicated that co-assembling with PVA could improve the uniaxial orientation of CNC arrays, which played a crucial role in enhancing the emission quantum efficiency (EQE). Stretching the PVA/CNC membrane could further induce an enhancement in EQE together with a blue shift in excitation wavelength. The mechanism study on stimulation-response suggested that the EQE enhancement and blue shift came from the change in uniaxial orientation degree and periodicity of the CNC assembly, respectively. Since the stimulation-responsive enhancement in EQE (from ca . 40 to 60%) can even be observed by naked eyes, we believe such cellulose-based materials can be widely used in optical sensors.
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Journal of Inorganic and Organometallic Polymers and Materials
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2022
·Vol. 32
·期 5
·1736-1743
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IF 3.518
Zhang Houpeng,Wu Xianming,Tian Yong,Wang Kaixuan,Tang Shiyu,Zhong Chaofan
Four novel polymeric metal complexes with a D–A–π–A motif, BDTT-PY-Cd, BDTT-PY-Zn, BDTT-PY-Cu and BDTT-PY-Ni, were designed, synthesized and characterized. These polymeric metal complexes were made up with Cd(II), Zn(II), Cu(II), Ni(II) complexes, thienylbenzo-[1,2-b:4,5-b'] dithiophene (BDTT) and the 8-quinolinol derivative, which were used severally as dye sensitzers’ auxiliary electron acceptors (A), electron donor (D) and π bridges as well as the acceptors (A). Under AM 1.5 irradiation (100 mW cm −2 ), the devices of dye sensitized solar cells (DSSC) based on four polymer complexes exhibited short-circuit photocurrent densities ( J sc ) of 17.45 mA cm −2 , 14.75 mA cm −2 , 13.94 mA cm −2 and 12.00 mA cm −2 , as well as attractive power conversion efficiencies (PCE) of were 9.73%, 8.02%, 6.82% and 6.12%, respectively. The photovoltaic conversion efficiency (PCE) and short-circuit photocurrent density ( J sc ) of BDTT-PY-Cd, BDTT-PY-Zn, BDTT-PY-Cu and BDTT-PY-Ni decrease in order because the radius and charge number of the metal ion affect the strength of the coordination bond between the metal ion and the ligand. These results provides a new way of development for efficient and stable dye sensitizers in the future.
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PLANTA
·
2022
·Vol. 255
·期 4
·1-13
·
IF 4.54
Han Xueyuan,Wang Xiaoyu,Shen Chi,Mo Yiwei,Tian Rungang,Mao Linchun,Luo Zisheng,Yang Huanyi
Main conclusion
Exogenous ABA played a positive role in the accumulation and biosynthesis of aroma components of postharvest kiwifruit after low-temperature storage, especially the esters production during ripening.
Abstract Low-temperature storage (LTS) generally affects the aroma formation associated with the decrease in aroma quality in kiwifruit. In this work, abscisic acid (ABA) treatment after LTS increased the production of aroma components in postharvest kiwifruit and enhanced the related enzyme activity, especially alcohol acyltransferase (AAT), branched amino acid transaminase (BCAT) and hydroperoxide lyase (HPL). Corresponding to the enzyme activity, the gene expression of AchnAAT , AchnADH , AchnBCAT and AchnHPL was significantly up-regulated by ABA. The principal component analysis further illustrated the differences in aroma components between ABA and the control. The positive correlation of aroma accumulation with the expression levels of AchnPDC and AchnLOX and the enzyme activities of BCAT and pyruvate decarboxylase (PDC) was also revealed by correlation analysis. In addition, the promoter sequences of the key genes involved in aroma biosynthesis contained multiple cis-elements (ABRE and G-box) of ABA-responsive proteins. Combining the transcriptome sequencing data, the promoting role of ABA signaling in the regulation of aroma biosynthesis of postharvest kiwifruit after LTS was discussed. This study would provide a reference for improving aroma quality of postharvest kiwifruit after LTS, as well the molecular mechanism of kiwifruit aroma fading after LTS.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2022
·Vol. 29
·期 32
·48522-48538
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IF 5.19
Wang Guanghui,Li Yingjie,Dai Jialing,Deng Nansheng
In this present study, a novel indirect Z-scheme TiO 2 @g-C 3 N 4 @biochar (TiO 2 @g-C 3 N 4 @BC) composite photocatalyst was successfully fabricated and characterized with SEM, TEM, EDS, XRD, FTIR, PL, XPS, and UV–vis DRS. The photocatalytic degradation behavior of ciprofloxacin (CIP) on the TiO 2 @g-C 3 N 4 @BC was evaluated under UV–vis and visible light irradiation, and the possible reaction mechanism of photocatalytic oxidation of CIP on the TiO 2 @g-C 3 N 4 @BC was explained. The TiO 2 @g-C 3 N 4 @BC composite photocatalyst exhibited stronger photocatalytic oxidation activity for CIP in comparison with TiO 2 , g-C 3 N 4 , TiO 2 @BC, and TiO 2 @g-C 3 N 4 . After 60 min of UV–vis and visible light irradiation, the photocatalytic removal efficiency of CIP by TiO 2 @g-C 3 N 4 @BC was 99.3 and 89.2%, respectively. The photocatalytic removal performance of CIP was affected by the initial concentration of CIP, catalyst dosage, and pH value. The composite photocatalyst presented excellent stability and reusability after five cycles. An indirect Z-scheme principle of the CIP photocatalytic oxidation reaction on TiO 2 @g-C 3 N 4 @BC was clearly proposed, and the whole process of photocatalytic degradation was the results of the interaction between CIP and reactive active species (·O 2 − , h + , and ·OH), of which ·O 2 − is the main active substance. Four CIP degradation pathways were proposed. This work may provide an effective strategy to remove antibiotics in wastewater.
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ARCHIVES OF MICROBIOLOGY
·
2022
·Vol. 204
·期 3
·1-12
·
IF 2.667
Ming Shanxiu,Chen Xujun,Zhang Na,Li Shuyi,Zhu Zhenzhou,Cheng Shuiyuan
Microorganisms can produce many antibiotics against bacteria and fungi, which have been used as a potential choice of new antibiotics. In this paper, we studied the characteristics of antibacterial substances by Bacillus cereus BC1. The results showed that the acid-precipitated substance played the main role in antibacterial activity, and further characterization indicated that the antibacterial substance might be a lipopeptide substance. Then the antibacterial spectrum suggested that the antibacterial substance had an inhibitory effect on Gram-positive bacteria and fungi, while selenium-riched antibacterial substance of Bacillus cereus BC1 could significantly enhance the inhibition. Then the morphological effects of the antibacterial substance to indicator bacteria were determined. The effects of different treatment methods on the stability of antibacterial substances were studied and the results showed that the antibacterial substance was stable to heat, ultrasonic, and ultraviolet treatment, and their antibacterial activity would not be greatly affected. However, they were sensitive to pepsin. The optimum pH range of antibacterial activity was 3–5. This study may contribute to reusing the fermentation supernatant often discarded in the previous fermentation process. At the same time, the lipopeptide antibacterial substance extracted from the fermentation broth of selenium-enriched Bacillus cereus BC1 can be used in the development of antibiotics and biopesticides, and open up a new way for the control of plant diseases.
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MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 3
·1-11
·
IF 6.408
Liu Zixuan,Yin Zheng-Zhi,Cai Wenrong,Wu Datong,Li Junyao,Kong Yong
A surface protein-imprinted biosensor was constructed on a screen-printed carbon electrode (SPCE) for the detection of anti-human immunoglobulin G (anti-IgG). The SPCE was successively decorated with aminated graphene (NH 2 -G) and gold nanobipyramids (AuNBs) for signal amplification. Then 4-mercaptophenylboric acid (4-MPBA) was covalently anchored to the surface of AuNBs for capturing anti-IgG template through boronate affinity binding. The decorated SPCE was then deposited with an imprinting layer generated by the electropolymerization of pyrrole. After removal of the anti-IgG template by the dissociation of the boronate ester in an acidic solution, three-dimensional (3D) cavities complementary to the anti-IgG template were formed in the imprinting layer of polypyrrole (PPy). The molecularly imprinted polymers (MIP)-based biosensor was used for the detection of anti-IgG, exhibiting a wide linear range from 0.05 to 100 ng mL −1 and a low limit of detection of 0.017 ng mL −1 (S/N = 3). In addition, the MIP-based anti-IgG biosensor also shows high selectivity, reproducibility and stability. Finally, the practicability of the fabricated anti-IgG biosensor was demonstrated by accurate determination of anti-IgG in serum sample.
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JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2022
·Vol. 26
·期 3
·791-798
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IF 2.747
Zhao Meiyu,Du Pin,Liu Wei,Du Kaifa,Ma Yongsong,Yin Huayi,Wang Dihua
Making a tantalum carbide (TaC) coating on Ta substrates is an effective way to improve the mechanical and anti-corrosion properties of Ta. However, conventional methods for making a TaC coating require high-temperature conditions of over 1600 °C to get a TaC thickness of > 20 μm. Herein, a TaC coating of > 20 μm thickness was prepared on a Ta substrate in molten CaCl 2 -CaC 2 at 850 °C for 4 h. In the molten salt electrolyzer, the Ta plate served as the anode where C 2 2− was oxidized and simultaneously reacted with Ta to form a TaC layer, and Ca 2+ was reduced and alloyed with C to form CaC 2 that can replenish the C 2 2− . The electrolytic TaC exhibited an average hardness of 962 HV that was 6 times of the bare Ta substrate. In the 3.5 wt% NaCl solution, the TaC coating displayed a higher corrosion potential and lower corrosion current density (0.48 V, 1.59 μA cm −2 ) than Ta substrate (0.35 V, 1.85 μA cm −2 ) and the mixtures of TaC and Ta 2 C (0.35 V, 1.78 μA cm −2 ), indicating its better corrosion resistance. The molten salt anodic carbidation may lead to a general way to prepare transition metal carbide coatings with enhanced carburizing kinetics at < 1000 °C. Graphical abstract
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2022
·Vol. 33
·期 4
·2116-2124
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IF 2.779
Li Chen,Hou Jiale,Ye Zijun,Muhammad Raz,Li Aihua,Ma Mingtao,Wu Guofa,Song Kaixin,Zhou Tao,Mao Minmin,Liu Bing,Bafrooei Hadi Barzegar,Taheri-nassaj Ehsan,Luo Shaojin,Shi Feng,Sun Shikuan,Wang Dawei
In this study, Mg x Y 3- x Al 5- x Si x O 12 ( x = 0–0.6) ceramics were prepared via a high-temperature solid state method. Mg 2+ and Si 4+ replacing Y 3+ ions at A-site and Al 3+ at C-site in garnet-type Y 3 Al 5 O 12 (YAG) ceramics were designed to discuss the effects of crystal structure, microstructure on microwave dielectric properties for the first time. Liquid phase sintering induced by doping of Mg 2+ -Si 4+ , significantly decreased the sintering temperature and time for the densification of YAG ceramics. X-ray diffraction of the samples showed single phase for x ≤ 0.5, while a secondary phase of MgAl 2 O 4 formed for x = 0.6. From the Rietveld analysis, a decrease in lattice dimensions, bond length and valence and rattling effect were observed, while Raman results showed a change in FWHM of the Eg mode. Based on these parameters, a structure–property relation was developed for Mg x Y 3- x Al 5- x Si x O 12 ceramics. Specially, Mg 0.1 Y 2.9 Al 4.9 Si 0.1 O 12 showed a good set of microwave dielectric properties sintered at 1575 °C for 4 h with ε r = 10.67, Q × f = 58,799 GHz, τ f = -29.5 ppm/°C.
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