BIOMEDICINE & PHARMACOTHERAPY
·
2020
·
IF 4.545
Gaojun Wu,Zhengxian Wang,Peiren Shan,Shanjun Huang,Shuang Lin,Weijian Huang,Zhouqing Huang
Background Myocardial remodeling caused by angiotensin II (Ang II) is essential for the pathological process of heart failure. Netrin-1, which is an axonal guidance cue, has been shown to be involved in the inflammatory response, tumorigenesis, and angiogenesis in non-neuronal tissues. However, the role of Netrin-1 in cardiac remodeling has not been fully elucidated. Methods The rat cardiomyocyte cell line H9c2 and primary neonatal rat cardiomyocytes were treated with Ang II. Cells were transfected with siRNA to silence Netrin-1 expression. Real-time polymerase chain reaction and Western blot analysis were used to detect the markers for fibrosis, apoptosis, and hypertrophy in cardiomyocytes. An Annexin V-EGFP/PI cell apoptosis detection kit was used to measure the level of apoptosis caused by angiotensin II. Results We found that Netrin-1 expression was upregulated in the H9c2 cells and the neonatal rat cardiomyocytes stimulated by Ang II. The increased Netrin-1 expression was decreased by valsartan to block AT1R. Importantly, the application of Netrin-1 siRNA significantly alleviated the degrees of myocardial hypertrophy, fibrosis (reflected by Myhc, collagen I, and TGF-β) and apoptosis (reflected by the level of Caspase 3, Bax, and Bcl-2) induced by Ang II. In addition, the silencing of Netrin-1 substantially decreased the phosphorylation of PKCα, JNK, and P38. We treated H9c2 cells with LY317615, SP600125, and SB203580, inhibitors of PKCα, JNK, and P38, respectively, thereby resulting in a substantial decrease in hypertrophy, fibrosis, and apoptosis. Conclusions Ang II produces cardiac hypertrophy, fibrosis, and apoptosis through the upregulation of Netrin-1 and the activation of the AT1R/PKCα/MAPK (JNK, P38) pathway. Suppression of Netrin-1 can relieve Ang II-induced cardiac remodeling via inhibition of the PKCα/MAPK (JNK and P38) signaling pathway. Thus, Netrin-1 may be a novel therapeutic target for Ang II-mediated cardiac remodeling.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2020
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IF 3.573
Hang Li,Yi Zhai,Xubin Zhang,Guojun Lv,Yu Shen,Xuqing Wang,Tao Jiang,Yuzhou Wu
Hierarchical iron-containing TS-1 zeolites (FTS) were successfully synthesized via post-treatment desilication over microporous Fe-TS-1, which were prepared through a one-step hydrothermal synthesis. The prepared samples were characterized by X-ray diffraction, N2 adsorption–desorption, field-emission scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, ultraviolet visible spectroscopy, and X-ray fluorescence spectrometry. The experimental results illustrated that the MFI structure of all the FTS samples remained unchanged after NaOH desilication and acid treatment. The newly created mesopores can be controlled by changing the amount of framework Fe, as the extraction of Si could be affected because of the inhibitory effect of iron species during the desilication process. The catalytic performance of the prepared samples in phenol hydroxylation with H2O2 was investigated. Among all the samples, FTS-50-D showed a highest phenol conversion of 45.2% and a dihydroxybenzene selectivity of 95.2%. Besides, FTS-50-D also showed good reusability for five recycling tests.
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RSC Advances
·
2020
·Vol. 10
·期 17
·10006-10016
·
IF 3.119
Ruimin Li,Shiyong Xing,Shuai Zhang,Minghan Han
H-beta zeolites of 100–200 nm (named BEA-L) and 20–30 nm (named BEA-S) were treated by chemical liquid deposition (CLD) of tetraethyl orthosilicate (TEOS) to improve the selectivity of 2-phenyl linear alkylbenzene (2-LAB) from benzene alkylation with 1-dodecene. The results indicate that H-beta zeolite with a smaller crystal size has a longer lifetime due to shorter channels and less diffusion limitation. The deposited SiO2 layers passivated the external surface acid sites of the zeolite and made the pores narrower. BEA-L lost more external Brønsted acid sites than BEA-S with the same added amount of TEOS, which was due to the severe aggregation of BEA-S grains. This increased passivation gave BEA-L increased 2-LAB selectivity. And when the added amount of SiO2 was 7.20 wt% of the parent zeolite, the selectivity of 2-LAB over BEA-L significantly increased from 41.9% to 54.7% while that of BEA-S only increased by 2%.
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JOURNAL OF THE ELECTROCHEMICAL SOCIETY
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2020
·Vol. 167
·期 6
·067525
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IF 3.721
Yi Xiu,Ruiping Luo,Baoqing Han,Lu Liu,Hongsu Wang
There is an urgent need to develop convenient, rapid and sensitive detection methods to determine antibiotic residues in food. New Delhi metal-β-lactamase-1 (NDM-1) is a β-lactamase enzyme that catalyzes removal of all β-lactam antibiotics other than aztreonam via a spectral hydrolysis mechanism. In the present study, a new biosensor based on NDM-1 was designed and tested to detect penicillin antibiotics namely penicillin sodium (Pen G) and ampicillin sodium (AMP) without using any pH redox probe. The glassy carbon electrode (GCE) was modified by electro-polymerization of methylene blue (PMB), and the biosensor (NDM-1/PMB/GCE) was prepared by immobilizing NDM-1 enzyme with glutaraldehyde (GA). Penicillin was detected by differential pulse voltammetry (DPV). This study seems to be one of the first kind to apply NDM-1 enzyme to detect antibiotic residues in food. It can be used repeatedly and can successfully detect penicillin in actual milk samples by spike recovery experiment.
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Catalysts
·
2020
·Vol. 10
·期 4
·377
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IF 3.52
Wei Yu,Wenfeng Han,Yongnan Liu,Jiaqin Lu,Hong Yang,Bing Liu,Haodong Tang,Aimin Chen,Ying Li
BaClxFyas well as BaF2and BaClF catalysts were prepared by solid-state reaction at room temperature with Ba(OH)2as the precursor and NH4F/NH4Cl as the F and Cl sources. The catalysts were applied for the dehydrochlorination of 1-chloro-1,1-difluoroethane to vinylidene fluoride at 350 °C. The industrial manufacture of vinylidene fluoride (VDF) is carried out at 600–700 °C, whereas the BaClxFycatalysts provided a promising pathway to produce VDF at much lower temperatures. Unfortunately, the selectivity of VDF over BaF2decreased from 94% to 84% along with the deactivation of the BaF2catalyst monotonically. In the presence of small amounts of Cl in BaF2, stabilized selectivity was achieved. Over BaCl0.05F0.95, BaCl0.1F0.9and BaCl0.25F0.75, no decrease in VDF selectivity was observed. Clearly, the presence of small amounts Cl during solid-state preparation inhibited the growth of BaF2crystalline significantly. Far smaller particles were achieved. The particle size, or more precisely, the crystal size of the barium catalyst played a major role in the catalytic performance. In addition to the crystal growth, the presence of small amounts of Cl during catalyst preparation changed the chemical state of Ba, and therefore the adsorption and activation of the C–Cl bond for HCFC-142b were altered.Keywords:barium chlorofluoride;catalytic pyrolysis;barium fluoride;dehydrochlorination;1-chloro-1,1-difluoroethane;vinylidene fluoride
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CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
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2020
·Vol. 98
·期 6
·
IF 1.946
Di Wang,Meilan Zhou,Yan Wang,Shiren Sun
Xerostomia is a self-conscious symptom. High-mobility group box 1 (HMGB1) promotes pro-inflammatory effects in many diseases. This study aimed to clarify the role of HMGB1 in Sjögren syndrome (SS)-triggered xerostomia. Nonobese diabetic (NOD)/Ltj mice were used to establish an SS-triggered xerostomia model. The results showed that saliva production was decreased and anti-Sjögren syndrome B (anti-SSB) level was increased in SS. PCR, Western blot, and immunohistochemistry experiments indicated that the HMGB1 and aquaporin 5 (AQP5) levels were enhanced and diminished in SS compared with those in the control, respectively. While the mice were treated with anti-HMGB1, xerostomia was reversed due to the elevated saliva production and reduced anti-SSB level. In addition, it was found that the inhibition of HMGB1 restrained the toll-like receptor 4 (TLR4)/nuclear factor kappa B (NF-κB) axis activation. The TLR4 and p-IκB levels were alleviated, while the IκBα and NF-κB p65 levels were augmented. The NF-κB p65 binding activity was attenuated via the electrophoretic mobility shift assay (EMSA) after anti-HMGB1 treatment. Moreover, the repression of HMGB1 facilitated the expression of AQP5. These findings demonstrate that suppression of HMGB1 ameliorates SS-triggered xerostomia via suppressing the HMGB1/TLR4/NF-κB signaling pathway and upregulating AQP5 expression.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2020
·
IF 5.774
Jiuli Han,Lu Bai,Shuangjiang Luo,Bingbing Yang,Yinge Bai,Shaojuan Zeng,Xiangping Zhang
Ionic liquids (ILs) are attractive and prospective materials used in membranes due to the nonvolatility, structure tunability, and the ability to tune the interface compatibility. The cobalt IL Bmim 2 Co(NCS) 4 possesses the porphyrin-like N4-coordinated cobalt center which could selectively and reversibly interact with O 2 . Herein the Bmim 2 Co(NCS) 4 as liquid-phase oxygen carrier was used to fabricate thin-film composite (TFC) membrane with the polymer of intrinsic microporosity (PIM-1). The microstructures, properties and gas separation performance of the membrane were compared with the TFC membrane which takes cobalt porphyrin (T(4-Cl)PPCo) as a carrier. We found that the addition of a small amount of T(4-Cl)PPCo can enlarge the d -spacing of packed chains of PIM-1 and significantly increase the fractional free volume (FFV) and the gas permeability of the membrane, but the selectivity is unsatisfactory. It is worth noting that adding Bmim 2 Co(NCS) 4 into the polymer can increase greatly the O 2 /N 2 selectivity which is higher than that of the membrane with T(4-Cl)PPCo. Although the addition of Bmim 2 Co(NCS) 4 decreases the d -spacing of packed chains of PIM-1, resulting in the slight decline of FFV and gas permeability of the membrane, the Bmim 2 Co(NCS) 4 /PIM-1 TFC membranes exhibit better O 2 /N 2 separation performance than the T(4-Cl)PPCo/PIM-1 TFC membranes in consideration of the Robeson upper bound.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2020
·
IF 5.774
Wenbo Chen,Zhenguo Zhang,Lei Hou,Cancan Yang,Hongcheng Shen,Kai Yang,Zhe Wang
This work mainly studies the CO 2 separation performance of a new type mixed-matrix membranes (MMMs) based on MOF-801 as the microporous filler and PIM-1 as the polymer matrix. MMMs was successfully formed via simple casting/solvent evaporation approach at room temperature. The samples were systematically characterized by SEM, XRD, TGA and FTIR, the permeability-selectivity of MMMs to CO 2 , O 2 and N 2 was studied by gas permeability test. The experimental results show that the uniform dispersion of the CO 2 -philic MOF-801 filler provides a channel for the rapid and selective transport of CO 2 , so the MOF-801/PIM-1 MMMs revealed much higher CO 2 permeability and CO 2 /N 2 ideal selectivity (CO 2 = 9686 Barrer, CO 2 /N 2 = 27, all selectivity in the paper is ideal) than pure polymer membrane. After 90-days aging test, the CO 2 permeability of MMMs remains above 70%, which reflects its good anti-aging performance. After 120 h continuous gas permeability test, it also shows good long-term working stability. The experimental results show that the good gas separation performance of MMMs exceeds the Robeson upper bound (2008), showing good application prospect for practical greenhouse gas CO 2 separation.
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CHEMICAL ENGINEERING JOURNAL
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2020
·
IF 10.652
Ihtisham Ul Haq,Shen Hui Li,Hong-Gang Zhen,Rehan Khan,Ao-Shuai Zhang,Zhi-Ping Zhao
For separating 1, 3-butadiene (BD) from BD/N 2 mixture, among numerous studied metal–organic frameworks-based adsorbents, Ni-ZIF-8 was comparatively selected here based on intelligent gravimetric analysis, simulation studies, facile synthesis, and very good IAST selectivity. Experimental results showed good butadiene uptake capacity of Ni-ZIF-8 of 7.41 mol/kg, which is 42% higher than pure ZIF-8. Additionally, Ni-ZIF-8 exhibits IAST selectivities of 2826 and 3302 at 0.1 bar, at 25 and 45 °C respectively, for 20% BD/N 2 adsorption. The molecular simulation results showed that Ni-ZIF-8 has binding energies of 65.25, 9.54 kJ/mol, while ZIF-8 has 56.07, 15.03 kJ/mol for butadiene, and nitrogen, respectively. This strongly supported the experimental results. To the best of our knowledge, Ni-ZIF-8 showed the best working capacity of 5.91 mol/kg for butadiene among all the reported adsorbents. Moreover, dynamic adsorption experiments revealed that butadiene could be recovered from BD/N 2 mixtures at room temperature, suggesting great potential for industrial BD/N 2 separation.
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MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Lulu Hao,Liying Zhao,Guangbao Li,Yingying Li,Ligang Ma,Yanju Liu,Wenbin Wang,Jinming Kong
Cytokeratin fragment antigen 21-1 (CYFRA 21-1) DNA is a significant biomarker with relation to non-small cell lung cancer. Here, we described an ultrasensitive electrochemical biosensor for detection of CYFRA 21-1 DNA (target DNA, tDNA) via surface-initiated reversible addition fragmentation chain transfer (SI-RAFT) polymerization-based DNA metallization signal amplification strategy. Specifically, 5′terminal modified thiol peptide nucleic acid (PNA) probes are immobilized on the gold electrode surface (by Au-S selfassembly) for the identification of specific tDNA sequences. After hybridization, the 4-cyano-4-(phenylcarbonothioylthio) pentanoic acid (CPAD) is tethered to the PNA/DNA duplexes by means of the recognized phosphate-Zr 4+ -carboxylate chemistry. Subsequently, in the situation of C 3 H 4 O as the monomer, SI-RAFT polymerization can bring numerous aldehyde groups sites for the subsequent silver mirror reaction. The acrolein-polymers act as reductant to reduce Ag + into Ag 0 , and then the silver particles are deposited on the polymer skeleton, which significantly amplifies the electrochemical signal. Under optimal conditions, the designed sensor for tDNA detection show a wide linear range from 100 aM to 1 nM, and the detection limit is as low as 0.89 aM. Moreover, the proposed biosensor exhibits prominent applicability for analysis of tDNA in serum samples and high selectivity for different mismatched oligonucleotide fragments. Given the proposed biosensor without extra natural enzyme or bio-labels, this method has enormous potential in bioanalysis.
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PHYTOMEDICINE
·
2019
·
IF 4.18
Dan Chen,Ya-Xian Wu,Yu-bao Qiu,Bin-bin Wan,Gang Liu,Jun-liang Chen,Mu-dan Lu,Qing-feng Pang
Background Hypoxia is commonly existed in tumors and lead to cancer cell chemo/radio-resistance. It is well-recognized that tumor hypoxia is a major challenge for the treatment of various solid tumors . Hyperoside (quercetin-3-O-galactoside, Hy) possesses antioxidant effects and has been reported to protect against hypoxia/reoxygenation induced injury in cardiomyocytes . Therefore, Hy may be attractive compound applicable to hypoxia-related diseases . Purpose This study was designed to determine the role of Hy in hypoxia-induced proliferation of non-small cell lung cancer cells and the underlying mechanism. Study Design and Methods A549, a human non-small cell lung cancer (NSCLC) cell line, was used in the present study. 1% O 2 was used to mimic the in vivo hypoxic condition of NSCLC. The potential mechanisms of Hy on hypoxia-induced A549 survival and proliferation, as well as the involvement of AMPK/HO-1 pathway were studied via CCK-8 assay, EdU staining, flow cytometry, qRT-PCR and western blot . Results We showed that pretreatment with Hy suppressed hypoxia-induced A549 survival and proliferation in dose-dependent manner. In terms of mechanism, hypoxia-treated A549 showed the lower AMPK phosphorylation and the reduced HO-1 expression, which were reversed by Hy pretreatment. Both AMPK inhibitor (Compound C) and HO-1 activity inhibitor (Zinc protoporphyrin IX) abolished Hy-evoked A549 cell death under hypoxia stimuli. Of note, Ferrous iron contributed to Hy-induced A549 cell death under hypoxia, while Hy had no effect on lipid peroxidation under hypoxia. Conclusion Taken together, our results highlighted the beneficial role of Hy against hypoxia-induced A549 survival and proliferation through ferrous accumulation via AMPK/HO-1 axis.
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RSC Advances
·
2020
·Vol. 10
·期 2
·1015-1020
·
IF 3.119
Xing Wei,Yu-Jia Wang,Tie-Qiang Ren,Hai-Yan Wang,Min Wei
Hydrothermal synthesis is a typical method for the preparation of TS-1. In the current study, agitation was introduced during the crystallisation stage, and nano-sized TS-1 with little anatase TiO2 was successfully synthesised in a short time (1–8 h). Furthermore, under rotational crystallisation conditions, a series of TS-1 samples was prepared with different crystallisation times, and the products obtained were investigated as catalysts for the oxidative desulfurisation of thiophene from a model fuel. All samples were characterised utilising X-ray diffraction (XRD), scanning electron microscopy (SEM), N2-adsorption, ultraviolet-visible (UV-Vis) spectroscopy and Fourier-transform infrared (FT-IR) spectroscopy techniques. It was found that under rotational crystallisation conditions, nano-sized TS-1 could be synthesised in a short time that had improved efficiency for the oxidative desulfurisation reaction of thiophene in comparison with TS-1 synthesised by static crystallisation for 24 h.
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