JOURNAL OF MATERIALS RESEARCH
·
2023
·Vol. 38
·期 8
·2213-2224
·
IF 2.7
Zhang Quanchao,Zhong Bingbing,Zhang Yichuan,Yang Zhiwei,Zhu Xiangbo,Wang Jie,Luo Honglin,Wan Yizao
Lack of bioactivity limits the applications of bacterial cellulose (BC) in biomedical fields. In this study, we report the facile preparation of a macroporous BC (PBC)/chondroitin sulfate (CS) scaffold using the ex situ method by adding CS solution into the suspension of BC fragments followed by crosslinking with 1-ethyl-3(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) and freeze drying. The PBC/CS scaffold was characterized for morphology, physicochemical properties, cell behavior, and capability of inducing mineral deposition. Results show that the PBC/CS scaffold presents improved mechanical properties, cell adhesion, and proliferation over the PBC scaffold. Moreover, the presence of CS greatly enhances the deposition of minerals on the PBC/CS scaffold, an indicator of bioactivity. The present study provides a simple methodology for improving the bioactivity of BC and the results of the present work suggest that the PBC/CS scaffold has potential for use in bone tissue engineering. Graphical Abstract
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Journal of Cardiovascular Translational Research
·
2023
·
IF 3.4
Wang Li,Wang Chuanhe,Sun Zhaoqing,Du Aolin,Shan Fei,Sun Zhijun
Myocardial ischemia/reperfusion (MI/R) injury induces myocardial damage and dysfunction. Increasing evidence has confirmed that circular RNAs (circRNAs) play crucial roles in regulating MI/R. Mmu-circ-0001380 has identified to be highly expressed in myocardium of MI/R mouse model. However, its biological function and molecular mechanism in MI/R injury are still unclear. Here, we demonstrated that knockdown of cric-0001380 attenuated myocardial injury of MI/R mice. In vitro , silence of circ-0001380 significantly enhanced viability, and inhibited apoptosis and oxidative stress in HL-1 cells under oxygen–glucose deprivation/reoxygenation (OGD/R). Mmu-miR-106b-5p interacted with circ-0001380, and suppressed the expression of pleckstrin homology domain and leucine rich repeat protein phosphatase 2 (Phlpp2). The miR-106b-5p/Phlpp2 axis mediated the effect of circ-0001380 on OGD/R-induced apoptosis through regulating the phosphorylation of p38, and further involved in regulating the viability and oxidative stress of HL-1 cells. In conclusion, circ-0001380 downregulation relieves MI/R injury via regulating the miR-106b-5p/Phlpp2 axis. Graphical Abstract The present study indicates that mmu-circ-0001380 exacerbates the myocardial ischemia/reperfusion injury through modulating the miR-106b-5p/Phlpp2 axis in vitro and in vivo .
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Nature Catalysis
·
2023
·Vol. 6
·期 8
·666-675
·
IF 37.8
Zhang Haochen,Li Chunsong,Liu Wenxuan,Luo Guangsheng,Goddard William A.,Cheng Mu-Jeng,Xu Bingjun,Lu Qi
Light alkane activation under mild conditions remains a substantial challenge. Here we report an aqueous reaction system capable of selectively converting light alkanes into corresponding olefins and oxygenates at room temperature and ambient pressure using Cu powder as the catalyst and O 2 as the oxidant. In ethane activation, we achieved a combined production of ethylene and acetic acid at a rate of 2.27 mmol g Cu −1 h −1 , with a combined selectivity up to 97%. Propane is converted to propylene with a selectivity up to 94% and a production rate up to 1.83 mmol g Cu −1 h −1 , while methane is converted mainly to carbon dioxide, methanol and acetic acid. On the basis of catalytic experiments, isotopic labelling experiments, spectroscopic insights and density functional theory calculations, we put forward mechanistic understandings in which the C–H bond is activated by the surface oxide species generated during the oxidation process, forming alkyl groups as key reaction intermediates.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·
IF 4.3
Chu Shushu,Xia Mingyuan,Xu Peng,Lin Dalei,Jiang Yuanyuan,Lu Yizhong
Although traditional Fe-based nanozymes have shown great potential, generally only a small proportion of the Fe atoms on the catalyst’s surface are used. Herein, we synthesized single-atom Fe on N-doped graphene nanosheets (Fe-CNG) with high atom utilization efficiency and a unique coordination structure. Active oxygen species including superoxide radicals (O 2 •− ) and singlet oxygen ( 1 O 2 ) were efficiently generated from the interaction of the Fe-CNG with dissolved oxygen in acidic conditions. The Fe-CNG nanozymes were found to display enhanced oxidase-like and laccase-like activity, with V max of 2.07 × 10 −7 M∙S −1 and 4.54 × 10 −8 M∙S −1 and K m of 0.324 mM and 0.082 mM, respectively, which is mainly due to Fe active centers coordinating with O and N atoms simultaneously. The oxidase-like performance of the Fe-CNG can be effectively inhibited by ascorbic acid (AA) or hydroquinone (HQ), which can directly obstruct the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB). Therefore, a direct and sensitive colorimetric method for the detection of AA and HQ activity was established, which exhibited good linear detection and limit of detection (LOD) of 0.048 μM and 0.025 μM, respectively. Moreover, a colorimetric method based on the Fe-CNG catalyst was fabricated for detecting the concentration of AA in vitamin C. Therefore, this work offers a new method for preparing a single-atom catalyst (SAC) nanozyme and a promising strategy for detecting AA and HQ.
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MICROCHIMICA ACTA
·
2023
·Vol. 191
·期 1
·1-12
·
IF 5.7
Liu Xinyu,Yu Yang,Xie Tianhua,Cao Zijin,Li Zhiyan,Li Yuejing,Gu Yingqiu,Han Caiqin,Yang Guohai,Qu Lulu
Monolayer g-C 3 N 4 -modified Au/Ag nanorods (g-C 3 N 4 /Au/Ag NRs) array is fabricated as a dual-function platform with high surface-enhanced Raman scattering (SERS) response and excellent photocatalytic degradation ability for bisphenol A (BPA) residues. FDTD simulation results of Au/Ag NRs proves that the electromagnetic field intensity is significantly enhanced at the gap of Ag NRs and Au NPs and the protrusion of Au NPs, which endows the arrays with excellent SERS activity. The arrays exhibit high sensitivity for rhodamine 6G (R6G) (LOD = 1.1 × 10 -11 mol/L) and high SERS enhancement (EF = 9.2 × 10 7 ). In addition, the g-C 3 N 4 /Au/Ag NRs could degrade ˃90% of BPA adsorbed on the substrate surface within 140 min under visible light irradiation, and maintains its SERS activity after repeated use for 4 times. The dual-function platform with high SERS response and excellent recycling capability is proved to be reliable and is very promising for monitoring of BPA residues in food. Graphical abstract
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MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 8
·1-8
·
IF 5.7
Huang Feng,Deng Lei,Wang Tingting,Zhang Aomei,Yang Minghui,Hou Yi
A bovine serum albumin (BSA)-assisted ultrasonication strategy was developed for the synthesis of CsPbBr 3 nanocrystals (NCs) with stable fluorescence properties in aqueous solution. Such a preparation method is simple, fast and does not require complex equipment. The results show that the synthesized CsPbBr 3 NCs are homogeneous in particle size and have good solubility and stability in water. The CsPbBr 3 NCs have been utilized as fluorescence probe for rapid detection of hydrogen sulfide (H 2 S) in human serum. The reaction of H 2 S with the lead sites on the surface of CsPbBr 3 NCs produces lead sulfide (PbS), resulting in the decrease of fluorometric intensity of CsPbBr 3 NCs. Our designed fluorescent assay has a linear S 2− detecting range of 10 ~ 800 nM with a detection limit of 7.05 nM. The assay was used to determine H 2 S in human serum with spiked recoveries ranging from 94.98% to 102.69%. This work opens new avenues for the application of halide lead perovskite in different biosensing areas. Graphical abstract
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FIBERS AND POLYMERS
·
2023
·Vol. 24
·期 9
·3075-3084
·
IF 2.5
Ahmed Adnan,Zhang Meng,Li Siqi,Xu Lan
Chitosan (CS), as a biocompatible and biodegradable nature polymer, has excellent antibacterial property. Poly(ethylene oxide) (PEO), as a biomaterial with thermal stability, biocompatibility and biodegradability, has good spinnability. Anthocyanin, as a natural colorant agent with different molecular structures under different pH values, has good antibacterial and antioxidant properties and can be applied as a pH indicator. Electrospun nanofiber membranes (NFMs) show great application prospects in food packaging due to their unique structural and functional advantages. In this work, anthocyanin/CS/PEO nanofiber membranes (NFMs) for food packaging were batch prepared by the spherical section free surface electrospinning (SSFSE), and their yield reached 5.8 ± 0.4 g/h. The morphology, structure and properties of CS-based NFMs were explored, which showed that they were hydrophilic and had excellent antibacterial activity against Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ). Among them, anthocyanin/CS/PEO NFMs exhibited better mechanical properties and high antioxidant activity. Furthermore, the color changes of anthocyanin/CS/PEO NFMs at different pH values were investigated, illustrating that they could be used as a visual monitoring food packaging, and their application effects in beef storage were studied.
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PARASITOLOGY RESEARCH
·
2023
·Vol. 123
·期 1
·1-6
·
IF 2
Wang Bingxiang,Zhao Ningning,Sun Lingyu,Tan Qianqian,Xiao Qianqian,Chen Junpeng,Li Jinxuan,Zhang Xiao,Zhao Xiaomin
Difficulties of in vitro culture and genetic manipulation of Eimeria tenella have hindered the screening of virulence factors in this parasite. In this study, the E. tenella rhoptry protein 30 (EtROP30) was expressed in Toxoplasma gondii (RH∆Ku80-EtROP30), and its effect on the proliferation and virulence of parasites was investigated. The results revealed that the expression of EtROP30 had no impact on the invasion and egress processes. However, the RH∆Ku80-EtROP30 strain formed larger plaques compared to the RH∆Ku80, indicating that the EtROP30 expression promotes T. gondii proliferation. Furthermore, the RH∆Ku80-EtROP30 strain exhibited greater pathogenicity, resulting in earlier mortality and shorter overall survival time compared to RH∆Ku80. These results imply that EtROP30 expression facilitates parasite intracellular proliferation and virulence in mice, suggesting that EtROP30 might be a candidate virulence factor of E. tenella.
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Biotechnology for Biofuels and Bioproducts
·
2023
·Vol. 16
·期 1
·1-13
·
IF 0
Gao Hui,Li Mengxuan,Wang Qing,Liu Tingting,Zhang Xian,Yang Taowei,Xu Meijuan,Rao Zhiming
ATP, an important cofactor, is involved in many biocatalytic reactions that require energy. Polyphosphate kinases (PPK) can provide energy for ATP-consuming reactions due to their cheap and readily available substrate polyphosphate. We determined the catalytic properties of PPK from different sources and found that PPK from Cytophaga hutchinsonii ( Ch PPK) had the best catalytic activity for the substrates ADP and polyP 6 . An extracellular–intracellular dual system was constructed to high-throughput screen for better catalytic activity of Ch PPK mutants. Finally, the specific activity of Ch PPK D82N-K103E mutant was increased by 4.3 times. Therefore, we focused on the production of L-theanine catalyzed by GMAS as a model of ATP regeneration. Supplying 150 mM ATP, GMAS enzyme could produce 16.8 ± 1.3 g/L L-theanine from 100 mM glutamate. When 5 mM ATP and 5 U/mL Ch PPK D82N-K103E were added, the yield of L-theanine was 16.6 ± 0.79 g/L with the conversion rate of 95.6 ± 4.5% at 4 h. Subsequently, this system was scaled up to 200 mM and 400 mM glutamate, resulting in the yields of L-theanine for 32.3 ± 1.6 g/L and 62.7 ± 1.1 g/L, with the conversion rate of 92.8 ± 4.6% and 90.1 ± 1.6%, respectively. In addition, we also constructed an efficient ATP regeneration system from glutamate to glutamine, and 13.8 ± 0.2 g/L glutamine was obtained with the conversion rate of 94.4 ± 1.4% in 4 h after adding 6 U/ mL GS enzyme and 5 U/ mL Ch PPK D82N-K103E , which further laid the foundation from glutamine to L-theanine catalyzed by GGT enzyme. This proved that giving the reaction an efficient ATP supply driven by the mutant enzyme enhanced the conversion rate of substrate to product and maximized the substrate value. This is a positively combination of high yield, high conversion rate and high economic value of enzyme catalysis. The mutant enzyme will further power the ATP-consuming biocatalytic reaction platform sustainably.
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WATER AIR AND SOIL POLLUTION
·
2023
·Vol. 234
·期 8
·1-13
·
IF 2.9
Chen Xi,Wang Shuaiyin,Li Danni,Wang Ruiqi,Yang Shaoming,Liu Yongxin,Xu Wenyuan,Zhang Jiali
The catalytic materials for sulfate radical-based advanced oxidation processes with the performance of high activity and stable had attracted significant attention in the field of sustainable wastewater treatment. Here, a novel adsorption-reactive composite catalyst with Fe 3 O 4 impregnated acrylamide-based hydrogel (PAM-AMPS/Fe 3 O 4 ) was designed and synthesized for methylene blue (MB) degradation based on persulfate advanced oxidation processes. For the acrylamide-based hydrogel, the monomer of 2-acrylamide-2-methyl-propanesulfonic acid (AMPS) was added to improve the adsorption performance of the composite, and the maximum adsorption capacity of the selected hydrogel was about 40 mg/g. In the presence of the persulfate activated system, the degradation efficiency of MB of PAM-AMPS/Fe 3 O 4 was significantly increased to a level of 94% in 20 min. This value was far superior to catalytic systems without the AMPS. This outstanding performance was benefiting from the synergetic effects of reduced aggregation of Fe 3 O 4 combined with the enhanced adsorption capacity of PAM-AMPS hydrogel. Their cooperative function promoted oxidative decomposition of MB. In addition, PAM-AMPS/Fe 3 O 4 exhibited a good stability and reusability, and it could maintain high degradation efficiency and activity after three successive cycling tests. And at the same time, the produced dominate oxidizing species for MB degradation in the system were identified as SO 4 •− by the radical quenching experiments. This work laid a foundation for applying PAM-AMPS/Fe 3 O 4 as a highly efficient and stable catalyst for future applications and provided new insights into the design and synthesis of environmentally benign and inexpensive persulfate activators.
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Journal of Sustainable Metallurgy
·
2023
·Vol. 9
·期 3
·1201-1214
·
IF 2.4
Tao Wenju,Yang Jiaxin,Chen Liyu,Zhang Yifan,Wu Shaohua,Li Jiaming,Liu Zhaoshun,He Jingui,Wang Zhaowen
The high-value, green and harmless purification of aluminum electrolysis comprehensive solid waste can not only reduce its harm to the environment and soil but also separate and recover the high-value rare elements. This study aims to develop a process for recovering and treating waste aluminum electrolytes by sulfuric acid roasting. The results of orthogonal experiments and single factor experiments indicate that under the premise of not decomposing sulfuric acid to produce secondary hazardous waste, the high-value element F in WAE can be effectively separated under the condition of calcination at 275 °C for 4 h and an acid to material ratio of 7, with a recovery rate of 87.31%. According to the mechanism obtained from the analysis and characterization results of SEM, XRD, and chemical analysis on the residual water leaching residue after roasting, the process of sulfuric acid roasting WAE is a multi-step separation process of fluorine: from Na 3 AlF 6 and Na 5 Al 3 F 14 to AlF 3 and Al 2 (SO 4 ) 3 . Each phase transformation process is accompanied by the precipitation of fluorine in the form of HF. Graphical Abstract
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WATER AIR AND SOIL POLLUTION
·
2023
·Vol. 234
·期 8
·1-18
·
IF 2.9
Yan Lianpeng,Zhou Keming,Zhang Rui,Zhu Xuedong
In this study, Medulla tetrapanacis (MT), a kind of herbaceous plant, was modified by phosphoric acid (H 3 PO 4 ) and cetyltrimethylammonium bromide (CTAB) to obtain the adsorbent MT-H 3 PO 4 -CTAB. It could quickly and efficiently remove the anionic azo dyes, Congo Red (CR) and Ponceau S (Po.S), from solution. For 500 mg/L CR and 150 mg/L Po.S dye solution, the removal efficiency could reach 84.52% and 95.29% within 10 min, and the maximum adsorption capacities of MT-H 3 PO 4 -CTAB towards CR and Po.S were 719.42 mg/g and 230.94 mg/g at 303 K, respectively. The effects of initial pH of the solution, temperature, initial dye concentration, and contact time were optimized. The adsorption process followed the pseudo-second-order kinetic model, and the equilibrium adsorption data fitted the Langmuir isotherm well. Moreover, thermodynamic studies indicated that the adsorption of two anionic azo dyes was spontaneous. The adsorption of CR onto MT-H 3 PO 4 -CTAB was endothermic while the adsorption of Po.S was exothermic. Molecular interactions, such as electrostatic attraction and hydrogen bonding between dyes molecules and adsorbent, play an important role in the adsorption process. This study demonstrates that acid-activated and surfactant-modified herbaceous plant adsorbent has a simple preparation process and exhibits excellent adsorption properties for anionic azo dyes, which could extend the potential applicability for biomass.
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