CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yongxian Wen,Menghan Xu,Yang Hu,Jiangkai Bao,Quanling Yang,Chuanxi Xiong,Zhuqun Shi
Poly(L-lactic acid) (PLLA), a piezoelectric material with excellent biocompatibility, has garnered significant attention due to its wide applications in wearable biosensors. Enhancing its piezoelectric performance has thus become a key research focus. In this study, we fabricated a composite electrospun membrane composed of Poly (L-lactic acid) (PLLA) and tetragonal barium titanate (T-BTO). By the strong stretching of the collector and incorporating the piezoelectric phase of T-BTO, the PLLA/submicron- sized T-BTO composite membranes generated an open-circuit voltage of 1.03 V and a short-circuit current of 33.5 nA. The submicron-sized T-BTO demonstrated significantly better dispersion and performance enhancement compared to the micron-sized T-BTO. Furthermore, flexible devices based on these membranes demonstrated sensitivity to joint movement, producing corresponding electrical signals. These findings suggest that PLLA/T-BTO electrospun fiber membranes hold great potential as flexible, sensitive piezoelectric materials for bio-sensing applications.
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ACS Applied Nano Materials
·
2024
·
IF 5.3
Yuan-Hui Yu,Wan-Long Li,Wan-Ting Lin,Ping Fu,Zhi-Kang Xu,Ling-Shu Wan
Nanofiltration (NF) membranes have aroused great attention in recent years. The most commonly used NF membranes are polyamide (PA) thin-film composite (TFC) membranes based on ultrafiltration membranes. Owing to the high porosity and interconnected pores, electrospun nanofibrous membranes are promising substrates for the fabrication of high-flux nanofiltration membranes, i.e., thin-film nanofibrous composite (TFNC) nanofiltration membranes. In this work, poly(l-lactic acid) (PLLA) nanofibrous membranes with adjustable hydrophilicity were successfully fabricated, and then, a PA layer was synthesized on the surface via the interfacial polymerization between piperazine (PIP) and trimesoyl chloride (TMC). The hydrophilicity of PLLA nanofibrous membranes influences the distribution and adsorption weight of PIP, which further affects the formation and morphology of the PA layer. Results indicate that, as the hydrophilicity of PLLA nanofibrous membranes increases, the adsorption amount of PIP increases, the thickness of the PA layer decreases, and the cross-linking degree increases, which ensures both high permeance and high rejection to Na2SO4. The TFNC membranes exhibit a water permeance of 17.0 L·m–2·h–1·bar–1 and a Na2SO4 rejection of 98.0%. This work achieves the control of substrate hydrophilicity by introducing hydrophilic additives and provides new insights for the fabrication of high-performance nanofiltration membranes.
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BIOTECHNOLOGY LETTERS
·
2024
·
IF 2
Zhang Fei,Wang Xue,Pan Lixia,Wang Zhao,Zheng Jianyong
The α- l -rhamnosidase ( rha 1) gene was homologously expressed in Aspergillus niger strains CCTCC 206047 and CCTCC 206047Δ pyr G, using hygromycin B and auxotrophic as selection markers. The engineered A. niger strains RHA001-1 and RHA003-1 were screened, yielding α- l -rhamnosidase activities of 20.81 ± 0.56 U/mL and 15.35 ± 0.87 U/mL, respectively. The copy numbers of the rha 1 gene in strains RHA001-1 and RHA003-1 were found to be 18 and 14, respectively. Correlation analysis between copy number and enzyme activity in the A. niger strains revealed that α- l -rhamnosidase activity increased with the copy number of the rha 1 gene. Recombinant α- l -rhamnosidase was utilized for the enzymatic debittering of Ougan juice, and its process conditions were optimized. Furthermore, the primary bitter substance neohesperidin (2.22 g/L) in Ougan juice was converted into hesperetin 7-O-glucoside (1.47 g/L) and hesperidin (0.143 g/L). This study presents a novel approach for the production of food-grade α- l -rhamnosidase and establishes a technical foundation for its application in the beverage industry.
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PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Cheng-Hai Yan,Ding Wang,Ruo-Xi Mei,Lu Tan,Yi-Jiang-Cheng Li,Richard Ansah Herman,Yan Xu,Lu-Chan Gong,Jun Wang
Natural flavonoids exhibit diverse physiological effects and are used as functional factors, antioxidants and pigments in food as well as pharmaceutical and cosmetic applications. However, their poor solubility and stability, result in low bioavailability. Biomodification through deglycosylation effectively enhances their bioactivity. This study developed two surface display strategies in Escherichia coli , anchoring α -L-rhamnosidase RhaB1-ΔN to the cell surface using the anchoring protein Lpp-OmpA. Immunofluorescence and proteinase K sensitivity tests confirmed the successful construction of surface-displaying strains E. coli Lpp-OmpA-RhaB1-ΔN and E. coli RhaB1-ΔN-SpyCatcher-SpyTag. Enzymatic property studies revealed improved temperature and pH stability of the whole-cell catalysts with surface compared to free enzymes. After 6 catalytic cycles, E. coli Lpp-OmpA-RhaB1-ΔN retained higher activity than E. coli RhaB1-ΔN. In flavonoid hydrolysis, E. coli Lpp-OmpA-RhaB1-ΔN increased yields of isoquercitrin and kaempferol-3- O -glucuronide by 20.25% and 10.99%, respectively, compared with that in whole-cell catalyst with intracellular enzyme ( p < 0.05). In addition, it should be noted that although the enzyme expression of E. coli RhaB1-ΔN-SpyCatcher-SpyTag is reduced, its hydrolysis ability of flavonoids is comparable to that of E. coli RhaB1-ΔN due to the efficient catalytic ability of extracellular enzymes. This study establishes reliable whole-cell catalysts with surface-displayed α -L-rhamnosidase for efficient natural flavonoid modification, with potential applications in the food and pharmaceutical industries.
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MOLECULES
·
2025
·Vol. 30
·期 5
·1055
·
IF 4.6
Qi Chen,Hui He,Yanghong Zhu,Xiang Li,Junhao Fang,Zhexi Li,Panghui Liu,Lin Zhou,Yufang Pan,Guoyu Wu
Individuals infected with COVID-19 often experience the distressing discomfort of pharyngitis. Thus, it is crucial to develop novel drugs to improve therapeutic options. In this study, we investigated the interaction between bioactive compounds isolated from Acanthopanax trifoliatus (L.) Merr and proteins associated with COVID-19 and pharyngitis through in silico analysis. Several molecules demonstrated high affinities to multiple targets, indicating significant potential for alleviating pharyngitis and other COVID-19-related symptoms. Among them, rutin and isochlorogenic acid C, two major components in Acanthopanax trifoliatus (L.) Merr ethanol extracts, were further experimentally demonstrated to exhibit strong inhibitory effects against SARS-CoV-2 and to possess significant anti-inflammatory activities. Inhibition of over 50% in several key genes was observed, demonstrating the efficacy of in silico methods in identifying high-affinity target binders. Our findings provide a theoretical foundation for the development of Acanthopanax trifoliatus (L.) Merr as a novel multi-target therapeutic agent for both COVID-19 and pharyngitis.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Lingyu Li,Zhichang Qiu,Xinyan Bai,Wenqing Zhu,Iftikhar Ali,Chunxia Ma,Zhenjia Zheng,Xuguang Qiao
Arctium lappa L. is widely consumed for its various biological effects, and polysaccharides are its main functional components. The present study aimed to evaluate the immunoregulatory effects of the main polysaccharides from burdock (ALP-1) and reveal the underlying mechanisms. ALP-1 consisted of fructose and glucose (14.57:1) and had a molecular weight of 2757 Da, with typical characteristics of (1 → 2)-linked linear fructans. Oral intake of ALP-1 significantly increased the number of colonic goblet cells, serum immunoglobulin A and immunoglobulin G levels, and fecal secretory immunoglobulin A content as well as up-regulated antioxidant enzymes and increased short chain fatty acid production. In addition, ALP-1 administration regulated pro/anti-inflammatory cytokines (i.e., interleukin (IL)-1β, IL-6, tumor necrosis factor-α, interferon-γ, and IL-10), intestinal microbiota structure, and the spatial information on key metabolites. Some gut-microbiota-mediated metabolic processes were also significantly altered. These results indicated that ALP-1 could exert beneficial effects on immune responses and intestinal health in healthy mice.
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PubMed
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Food Bioscience
·
2025
·
IF 5.9
Bingjie Wang,Pengfei Jiang,Lihua Zhang,Zhaogai Wang,Wei Zong
The dynamic changes in key enzyme activities, physicochemical properties and volatile compounds of garlic cloves were evaluated after storage at -20, 4, 25 and 60 °C for 64 d and 28 d, respectively. Samples stored at -20 °C exhibited no significant changes in physicochemical properties. A rapid increase in gamma-glutamyl transferase (γ-GT) activity was observed in samples stored at 4 °C. Furthermore, samples stored at 25 °C or 60 °C facilitated the development of reducing sugar, total acids, total polyphenols and superoxide dismutase with prolonged storage time. Gas chromatography-mass spectrometry (GC-MS) identified 69 volatile compounds in garlic cloves treated at 4 °C and 25 °C, with sulfur-containing compounds (46 types) being the primary components of the garlic clove aroma profile. Interestingly, garlic with a strong spicy flavor developed a rich fruity aroma during storage at 25 °C. This may be related to the increase in the content of ester compounds such as (E)-2-butenoic acid ethyl ester, ethyl tiglate and β-phenylethyl butyrate. Correlation analysis suggested that peroxidase activity was positively correlated with the content of free amino acids (r=0.972, p <0.01) and reducing sugar (r=0.724) in garlic cloves stored at 4 °C, while the activity of γ-GT (-0.969
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Foods
·
2024
·Vol. 13
·期 11
·1727
·
IF 4.7
Chengyu Lu,Ziwei Chen,Yuvaraj Ravikumar,Guoyan Zhang,Xinrui Tang,Yufei Zhang,Mei Zhao,Wenjing Sun,Xianghui Qi
L-Arabinose isomerase (L-AI) has been commonly used as an efficient biocatalyst to produce D-tagatose via the isomerization of D-galactose. However, it remains a significant challenge to efficiently synthesize D-tagatose using the native (wild type) L-AI at an industrial scale. Hence, it is extremely urgent to redesign L-AI to improve its catalytic efficiency towards D-galactose, and herein a structure-based molecular modification ofLactobacillus plantarumCY6 L-AI (LpAI) was performed. Among the engineered LpAI, both F118M and F279I mutants showed an increased D-galactose isomerization activity. Particularly, the specific activity of double mutant F118M/F279I towards D-galactose was increased by 210.1% compared to that of the wild type LpAI (WT). Besides the catalytic activity, the substrate preference of F118M/F279I was also largely changed from L-arabinose to D-galactose. In the enzymatic production of D-tagatose, the yield and conversion ratio of F118M/F279I were increased by 81.2% and 79.6%, respectively, compared to that of WT. Furthermore, the D-tagatose production of whole cells expressing F118M/F279I displayed about 2-fold higher than that of WT cell. These results revealed that the designed site-directed mutagenesis is useful for improving the catalytic efficiency of LpAI towards D-galactose.
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CHEMISTRY OF MATERIALS
·
2024
·
IF 7.2
Jing Chen,Jiao Yang,Wenjing Liu,Jianbin Dong,Ying Chen,Lianqin Zhu,Rong Deng,Xiaoquan Lu
l-tryptophan (l-Trp) plays an important role in human and animal metabolism, and an imbalance or deficiency can cause some chronic diseases. While d-tryptophan (d-Trp) is mainly present in microorganisms and green plants and is rarely found in animals. In this work, a simple and efficient electrochemical sensing platform for the simultaneous detection of tryptophan enantiomers based on an l-lysine-functionalized nickel–zinc bis(dithiolene) metal–organic framework and ferrocene-modified multiwalled carbon nanotubes (Fc-MWCNTs) is presented. The identification effect of l-Lys-Ni Zn-MOF/Fc-MWCNTs/GCE on tryptophan (Trp) enantiomers was studied by differential pulse voltammetry. The synthesized materials have been successfully applied to the detection and analysis of Trp enantiomers, and the enantiomer selection coefficient (IL/ID) detected by the sensor is 1.866, indicating that l-Lys-Ni Zn-MOF/Fc-MWCNT composites have good application potential.
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EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
·
2024
·
IF 1.8
Yikang Zhang,Chenwei Zhao,Jun Jin,Qingzhe Jin,Xingguo Wang
In the present study, antioxidant impacts of L-ascorbyl palmitate (L-AP) on 14 kinds of typical vegetable oils were studied, whose ratios of C18:1 to C18:2 ranged from 0.15 to 9.57. Regression analyses between vegetable oils and their micronutrient-free counterparts revealed that the antioxidant capacity of L-AP was closely related to concentrations of α-, γ-, and δ-tocopherols instead of fatty acid compositions. Binary mixtures consisting of L-AP and α-, γ-, or δ-tocopherols exhibited desirable synergistic antioxidant effects in comparison with individual species. The optimal synergistic effects were found at ratios of L-AP to α-, γ-, δ-tocopherols, being 1:2–1:4. Further EPR spin trapping test figured out that the combined antioxidants reduced the total amount of spin adducts significantly during lipid oxidation, which were contributed by regenerating tocopherols from L-AP. The results will highlight the application of L-AP in protecting packed cooking oils after opening and during cooking. Practical applications : Vegetable oils packaged in 4–5 L bottles are one of the most important forms for home cooking. Although deoxygenation techniques are applied to increase their shelf life during storage, they will experience a more violent oxidation reaction after opening. The oils are typically oxidized to rancidity before ending up in consumption. L-AP was therefore involved into the vegetable oils to prevent them from oxidation during uses. The results of study can help companies to select the optimal L-AP addition amount according to different endogenous tocopherol types and contents in the target oil in order to extend the use life of the vegetable oil.
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Journal of Water Process Engineering
·
2025
·
IF 6.7
Yue Su,Chun Wang,Ju Wang,Yunqi Liu,Haibo Chang,Mingxue Li,Haichao Zhao
The labyrinth effect of graphene coatings could extend the diffusion path of corrosive media. However, the poor compatibility between graphene and resins and the low loading capacity of graphene affect its functional application. By combining the in-situ synthesis and electrostatic interaction, layered double hydroxides (LDH) were grown on the graphene surface, L-aspartic acid anions (Asp) were adsorbed on LDH by electrostatic interactions. The loading capacity of L-aspartic acid was up to 40.5 wt%. The barrier property of nanohybrid coatings was measured by electrochemical workstation, it was found that the rGO-LDH-Asp/epoxy coating exhibited the best corrosion protection properties, and the coating resistance could still be maintained at 1.231 × 10 9 Ω⋅cm 2 after being immersed in 3.5 wt% NaCl solution for 90 days. The anticorrosion mechanism of the coating was explored through local electrochemical impedance spectroscopy, L-aspartic acid anions adsorbed on the surface of rGO-LDH could accelerate the passivation of the metal substrate and delay the corrosion process.
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Current Research in Food Science
·
2024
·
IF 6.2
Ouyang Zheng,Xiaojie Cao,Yanqing Teng,Qinxiu Sun,Shucheng Liu
The effects of dense phase carbon dioxide (DPCD) pressure and temperature on the activity of cathepsin L in white shrimp ( Litopenaeus vannamei ) were studied. Meanwhile the effects of DPCD and heat treatment on the activity of cathepsin L were compared. The results of inactivation kinetics showed that under the same treatment temperature, the inactivation effect of DPCD on cathepsin L increased gradually with the increase of pressure and time. The effect of DPCD pressure on the activity of cathepsin L accorded with the first-order kinetic model. Under the same treatment pressure, with the increase of temperature, the inactivation effect of cathepsin L was significant at the initial stage of DPCD treatment (rapid inactivation period), and decreased with the extension of time (stable inactivation period). The effect of DPCD treatment temperature on cathepsin L activity accorded with the first-order kinetic model at 35 °C, and two-stage kinetic model at 40–60 °C. The difference of relative enzyme activity between the two treatments showed that the dependence of DPCD on temperature was greater than that of heat. E a, F and E a, S of DPCD were higher than that of heat, which indicated that cathepsin L was more easily inactivated under DPCD treatment.
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高影响因子
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