ENZYME AND MICROBIAL TECHNOLOGY
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2024
·
IF 3.4
Bohua Wang,Song Lei,Qingqin Li,Yushuang Luo
Lactulose is a semisynthetic nondigestive sugar derived from lactose, with wide applications in the food and pharmaceutical industries. Its biological production routes which use cellobiose 2-epimerase (C2E) as the key enzyme have attracted widespread attention. In this study, a set of C2Es from different sources were overexpressed in Escherichia coli to produce lactulose. We obtained a novel and highly efficient C2E from Clostridium disporicum (CDC2E) to synthesize lactulose from lactose. The effects of different heat treatment conditions, reaction pH, reaction temperature, and substrate concentrations were investigated. Under the optimum biotransformation conditions, the final concentration of lactulose was up to 1.45 M (496.3 g/L), with a lactose conversion rate of 72.5 %. This study provides a novel C2E for the biosynthesis of lactulose from low-cost lactose.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2024
·
IF 9.4
Ziyu Gan,Jun Wang
Plants exhibit rapid responses to biotic and abiotic stresses by releasing a range of volatile organic compounds (VOCs). Monitoring changes in these VOCs holds the potential for the early detection of plant diseases. This study proposes a method for identifying late blight in potatoes based on the detection of (E) -2-hexenal, one of the major VOC markers released during plant infection by Phytophthora infestans . By combining the Michael addition reaction with cysteine-mediated etching of aggregation-induced emission gold nanoclusters (Au NCs), we have developed a portable hydrogel kit for on-site detection of (E) -2-hexenal. The Michael addition reaction between (E) -2-hexenal and cysteine effectively alleviates the etching of cysteine-mediated Au NCs, leading to a distinct fluorescence color change in the Au NCs, enabling a detection limit of 0.61 ppm. Utilizing the superior loading and diffusion characteristics of the three-dimensional structure of agarose hydrogel, our sensor demonstrated exceptional performance in terms of sensitivity, selectivity, reaction time, and ease of use. Moreover, quantitative measurement of (E) -2-hexenal was made easier by using ImageJ software to transform fluorescent images from the hydrogel kit into digital data. Such method was effectively used for the early detection of potato late blight. This study presents a low-cost, portable fluorescent analytical tool, offering a new avenue for on-site detection of plant diseases.
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ChemistrySelect
·
2024
·Vol. 9
·期 3
·e202303342
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IF 1.9
Nianming Jiao,Junjie Cui,Long Liu,Yuan Yao,Yangfeng Xia,Yanqiang Zhang
Graphical Pyridinium trifluoroacetate-based ILs effectively catalyzed the cyclization of 2-propyl ketazine to pyrazolines, achieving conversion and selectivity rates of 98 % and 99 %, respectively. Thermodynamic data were obtained through DFT calculations and calorimeter tests, providing valuable insights for the practical application of ketazine intramolecular cyclization to synthesize pyrazolines. In this work, pyridinium trifluoroacetate ionic liquids, as a protonic acid catalyst, were used to catalyze intramolecular cyclization of ketazine to synthesize 2-pyrazoline. The investigation encompassed the impact of temperature, solvent, dosage of ionic liquid catalyst, and the structure-activity relationship in the cyclization reaction of 2-propyl ketazine. The optimal catalytic performance was achieved at 100 °C with 8 mol % of 3,5-dimethylpyridinium trifluoroacetate in a solvent-free environment. Moreover, thermodynamic and kinetic data were determined through density functional theory calculations and calorimetric experiments. These protonic acid ionic liquids address the issue of excessive traditional protonic acid dosage in the intramolecular cyclization of ketazine, while also presenting a viable pathway for the catalytic synthesis of 2-pyrazolines.
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Nature Cancer
·
2025
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IF 28.5
Han Jinpeng,Wang Shenqiang,Fang Weifeng,Yang Yinxian,Zhou Ruyi,Zhang Yuqi,Yu Jicheng,Tang Ruikang,Liu Zhaoming,Gu Zhen
Long-acting controlled drug release formulations are highly desired for potentiating efficacy and reducing administration frequency. Here we present a kinetically controllable long-term interleukin-2 (IL-2) release platform by the fusion and boundary elimination of calcium carbonate and calcium phosphate amorphous phases. Unlike mixtures, a group of hybrid biominerals with the chemical formula Ca(CO 3 ) x (PO 4 ) 2(1− x )/3 (CaCPs, 0 < x < 1) was fabricated under high pressure (2 GPa), and the CaCPs showed crystallization-driven release behaviors to optimize the in vivo fate of IL-2. Ca(CO 3 ) 1/2 (PO 4 ) 1/3 dynamically remodeled immunosuppressive tumor microenvironments, preferentially activated cytotoxic and memory T cells by improving IL-2 redistribution and achieved weeks-long IL-2 retention in tumors with high tolerance and biosafety. In a melanoma model in female mice, Ca(CO 3 ) 1/2 (PO 4 ) 1/3 revealed superior antitumor effects to inhibit local tumor recurrence, hinder the growth of distant untreated tumors and maintain long-term T cell responses against the rechallenged metastatic tumors.
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Catalysts
·
2025
·Vol. 15
·期 9
·826
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IF 4
Anran Zhu,Zhufan Zou,Yu Cong,Yinghua Yin,Ning Li
A novel two-step strategy was developed for the efficient synthesis of decalin and octahydroindene from lignocellulose-derived platform compounds. In the first step, bicyclic intermediates were directly generated via a cascade dehydration/Robinson annulation of 4-hydroxy-2-butanone with cyclohexanone (or cyclopentanone). Among the evaluated catalysts, CaO demonstrated the highest activity and selectivity. Based on CO2-TPD results, the excellent performance of CaO can be rationalized by its proper basicity. In the second step, these intermediates were selectively hydrodeoxygenated to decalin (or octahydroindene) over Ni/H-ZSM-5 catalyst. Under the investigated reaction conditions, ~90% overall yields of decalin and octahydroindene were achieved. This work provides a viable strategy for the selective conversion of lignocellulose-derived platform compounds to the additives for improving the thermal stability of aviation fuel.
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PEST MANAGEMENT SCIENCE
·
2025
·
IF 3.8
Hamza Tariq,Peiyu Chen,Gang Wang,Siyuan Xie,Lanning Wang,Jingkun Lv,Fei Wang,Muhammad Saqib Bilal,Abdur Rashid Khan,Nasir Ahmed Rajput,Danyu Shen,Daolong Dou
BACKGROUND Alternaria alternata is a globally distributed plant pathogen infecting >400 plant species. Trans -2-decenal is a notable plant-derived secondary metabolite with strong volatility and antifungal properties, although its specific activity and mode-of-action against A. alternata remain unclear. RESULTS Toxicity assays showed that trans -2-decenal inhibited A. alternata with a median inhibitory concentration (IC 50 ) of 12.23 mg L −1 . Treated with trans -2-decenal resulted in hyphal abnormalities, reduced spore production and suppressed spore germination. Mechanistically, it disrupted fungal cell membrane integrity, as confirmed by propidium iodide staining and increased leakage of DNA and soluble proteins in conductivity assays. Further investigations revealed that trans -2-decenal disrupted redox homeostasis by inducing reactive oxygen species (ROS) accumulation. DCFH-DA fluorescence staining showed elevated ROS levels, whereas antioxidant enzyme assays revealed dose-dependent changes, including decreased superoxide anion (O 2 . − ) and catalase (CAT) activity, and significantly increased hydrogen peroxide (H 2 O 2 ), superoxide dismutase (SOD) and malondialdehyde (MDA) levels, collectively indicating oxidative stress and damage. Transcriptome analysis identified 2319 differentially expressed genes (DEGs), with gene ontology (GO) enrichment highlighting inhibition of pathways associated with oxidative stress response, transmembrane transport and ribosome biogenesis. Molecular docking further suggested interactions between trans -2-decenal and key antioxidant enzyme and membrane transporter of A. alternata . In vivo assays showed that 32 mg L −1 trans -2-decenal fumigation achieved protective and curative efficacies of 97.14% and 96.54%, respectively, against yam leaf spot disease. CONCLUSION Trans -2-decenal inhibited A. alternata through a multitarget synergistic mechanism, providing theoretical support for its development as a plant-derived biofumigant and a promising tool for the green management of A. alternata -induced diseases. © 2025 Society of Chemical Industry.
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ADVANCED MATERIALS
·
2025
·
IF 26.8
Anquan Zhu,Heng Liu,Lulu Qiao,Bin Liu,Kunlun Liu,Chuhao Luan,Kai Liu,Yin Zhou,Dewu Lin,Guoqiang Gan,Jiapei Li,Guo Hong,Wenjun Zhang
The electrochemical nitrate reduction reaction (eNO 3 RR) is widely recognized as a promising strategy for sustainable ammonia production and supporting the nitrogen cycle. However, its advancement is impeded by complex behavior of reaction intermediates and the inevitable reconstruction of precatalysts. To address these challenges, the generation and utilization of active hydrogen (*H) are strategically managed by tailoring the RuO 2 /Co 3 O 4 precatalyst and optimizing the electrolyte composition (OH − and NO 3 − concentration), thereby selectively enhancing ammonia formation. Consequently, the in situ reconstructed RuO 2 /Co(OH) 2 catalyst achieves an impressive ammonia yield of 35.9 ± 0.9 mg h −1 cm −2 and a Faradaic efficiency (FE) of 98.1 ± 2.6% at −0.3 V versus RHE. Furthermore, the catalyst shows significant potential for applications in nitrate-rich wastewater treatment and rechargeable Zn-NO 3 − batteries, maintaining stable operation for over 260 hours at 1 mA cm −2 with only a 6 mV increase in the potential window. Mechanistic studies reveal that electron-rich RuO 2 facilitates *H generation through water dissociation, which subsequently migrates to Co(OH) 2 to hydrogenate nitrogenous intermediates, selectively producing ammonia. This study highlights the importance of designing efficient catalytic systems that address both precatalyst reconstruction and the complexities of reactant and intermediate conversion in electrolytes, which are essential for managing the intricate electron and proton transfer processes involved in eNO 3 RR.
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ADVANCED MATERIALS
·
2025
·
IF 26.8
Si Sun,Fangzhen Tian,Huanhuan Qiao,Qi Xin,Xinxu Zhang,Nan Song,Yuxing Yan,Ling Liu,Yili Wang,Lijie Zhang,Ke Chen,Jiang Yang,Shu Zhang,Jianning Zhang,Yonghui Li,Hao Wang,Xiao-Dong Zhang
Type 2 diabetes mellitus is the most prevalent disease in the world, with one-tenth of the population suffering from the disease, and the most critical challenges are its complications that induce high disability and mortality rates. The state-of-the-art therapeutic agents can manage glucose but fail to prevent renal failure as well as neurodegeneration with immunosuppression. Herein, we developed a deep learning design strategy that exploits the ‘size-fitting effect’ to engineer an atomic-precision metal cluster for preventing diabetic complications by targeting metabolic abnormality and immunosuppression. The designed AuZn cluster achieves almost 100% α-amylase inhibition and 88% α-glucosidase inhibition, resulting in the normalized glycated hemoglobin and sustained glucose control. The intrinsic redox properties reduce oxidative stress damage, promoting β-cell regeneration and metabolic stress alleviation. Consequently, the renal function, the most prevalent complications, shows that glomerular filtration can be restored to normal levels without urinary protein, while the clinical dulaglutide is not show any improvement. The key marker during early neurocognitive disorders, the amyloid precursor protein (APP) induced by complications, can be effectively suppressed, and diabetes induced organelle degeneration in neurons can be restored.
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JOURNAL OF HETEROCYCLIC CHEMISTRY
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2023
·
IF 2.4
Ping Zhao,Qingle Zeng
We have developed a diversity-oriented, substrate-controlled, chemoselective synthetic method for a variety of structurally diverse 1-sulfonylquinazoline-2,4(1H,3H)-diones and 2-sulfonamidobenzonitriles. This substrate-controlled synthesis is accomplished through the condensation reaction of 2-(sulfonamido)benzamides with or without an N-methyl group on the amides. The reactions are fulfilled within half an hour at room temperature with solid triphosgene as a convenient condensing agent and pyridine as an acid acceptor. This protocol features easily available starting materials, mild reaction conditions, easy experimental operation, and moderate to excellent yields of products.
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LUMINESCENCE
·
2014
·Vol. 30
·期 2
·228-234
·
IF 1.675
Xi Yang,Tao Zhou,Lei Yu,Wenwen Tan,Rui Zhou,Yonggang Hu
A competitive chemiluminescence enzyme immunoassay (CLEIA) method for porcine β-defensin-2 (pBD-2) detection in transgenic mice was established. Several factors that affect detection, including luminol, p -iodophenol and hydrogen peroxide concentrations, as well as pH, were studied and optimized. The linear range of the proposed method for pBD-2 detection under optimal conditions was 0.05–80 ng/mL with a correlation coefficient of 0.9960. Eleven detections of a 30 ng/mL pBD-2 standard sample were performed. Reproducible results were obtained with a relative standard deviation of 3.94%. The limit of detection of the method for pBD-2 was 3.5 pg/mL (3σ). The proposed method was applied to determine pBD-2 expression levels in the tissues of pBD-2 transgenic mice, and compared with LC-MS/MS and quantitative real-time reverse-transcriptase polymerase chain reaction. This suggests that the CLEIA can be used as a valuable method to detect and quantify pBD-2. Copyright © 2014 John Wiley & Sons, Ltd.
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BIOORGANIC CHEMISTRY
·
2020
·
IF 4.831
Bo Zhang,Xiu-Ting Hu,Kang-Min Zhou,Yu-Shun Yang,Hai-Liang Zhu
Cyclooxygenase is critical for maintaining physiological functions, whereas overexpression of COX-2 was closely implicated in various cancers. In this study, a series of novel aminophosphonate derivatives containing pyrazole moiety were synthesized with their anti-cancer activity evaluated. In vitro assays of the target compounds showed that Z21 displayed excellent COX-2 inhibitory activity against COX-2 (IC 50 = 0.22 ± 0.04 µM) and anti-proliferative activity against MCF-7 cell (IC 50 = 4.37 ± 0.49 µM). The apoptosis induction of compound Z21 was confirmed by flow cytometry and polymerase chain reaction. Further investigation demonstrated that compound Z21 induced apoptosis of MCF-7 cells through a mitochondrion-dependent pathway and involved cell-cycle arrest in G2 phase. Overall, these results provided some new insights into the design of therapeutic drugs for COX-2 inhibitors and indicated the connection between selective COX-2 inhibition and the anti-tumor activity.
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EUROPEAN JOURNAL OF PHARMACOLOGY
·
2020
·
IF 3.263
Min Chen,Qiu-Ping Zhang,Ji-Xiao Zhu,Jie Cheng,Qing Liu,Guang-Hui Xu,Cheng-Fu Li,Li-Tao Yi
It has been reported that liquiritin produced an antidepressant-like effect in rodents. However, little information is known regarding its antidepressant activity with the regulation of fibroblast growth factor 2 (FGF-2), a protein maintaining development and maturation of the nervous central system . Therefore, the aim of the present study was to investigate the underlying FGF-2 modulation involved in the antidepressant-like effects of liquiritin. In the present study, mice were orally administrated with liquiritin for 7 days prior to LPS injection. The depressive-like behaviors , levels of FGF-2, number of Iba1 positive cells, expression of proinflammatory cytokines and density of dendritic spines were evaluated. The results showed that liquiritin significantly ameliorated the depressive-like behaviors in mice response to LPS injection. Liquiritin reversed the reduction of FGF-2 levels in the hippocampus of LPS induced mice. In addition, the microglial activation caused by LPS was attenuated by liquiritin, in accordance with downregulation in mRNA levels of proinflammatory cytokines. Moreover, liquiritin also increased the density of dendritic spines in the hippocampus, which was suppressed by LPS. In conclusion, our findings demonstrated that liquiritin exerted the antidepressant-like effects in LPS-induced depression through FGF-2 enhancement by inhibiting neuroinflammation and maintaining synaptogenesis .
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