ANALYTICA CHIMICA ACTA
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2020
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IF 5.977
Yue Zhang,Linfang Li,Ye Gao,Xinnan Wang,Lei Sun,Wei Ji,Yukihiro Ozaki
Urinary 5-hydroxyindole-3-acetic acid (5-HIAA) is a marker for diagnosis of patients with carcinoid tumors. In clinical practice, a simple colorimetric assay has widely been utilized for urinary 5-HIAA via its reaction with 1-nitroso-2-naphthol (NNa) in nitrous acid medium. However, this colorimetric assay has been criticized for lack of specificity. Herein, we proposed a novel SERS-based method for selective detection of urinary 5-HIAA by the use of a modified nitrosonaphthol reaction, in which hydrochloric acid is substituted for nitric acid to control the reaction conditions. This slight modification is crucial to be able to detect the cationic products in the acidic reaction mixture, because the chloride-aggregated Ag colloids not only create enormous hot spots for SERS enhancement, but also improve the chemical stability of nanostructured Ag substrates in acidic environment. Under optimized conditions, the present method can detect 5-HIAA within 12 min at concentrations as low as 1.2 ng/mL, which is 1000 times lower than that of conventional colorimetric method . And more importantly, the present method exhibits specific response toward 5-HIAA against other metabolites with similar structures in the urine. The relative standard deviation (RSD) of the present method is less than 5%, suggesting the acceptable reproducibility. The recoveries ranging from 89.6% to 106.3% were obtained for spiked human urine samples with RSD of 3.7–4.9%. Furthermore, several healthy person’s urine samples were also analyzed using the present method, and experimental results are in compliance with the levels recorded in a healthy population. On the basis of these results we can conclude that the present SERS-based method can provide a valuable alternative to conventional colorimetric assay for clinical diagnosis, evaluating prognosis, and monitoring of treatment in carcinoid tumors.
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高影响因子
Polymers
·
2022
·Vol. 14
·期 23
·5233
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IF 4.967
Chunhua Lou,Siyu Jiang,Yongli Zhou,Xiaohua Gu,Yong Zhang,Xianzhi Kong
A modified corn straw (CS)/epoxy resin (EP) composite was prepared using bisphenol A EP (i.e., E-51) as matrix, 2-methylimidazole as curing agent, and CS modified by 3-glycidyl ether oxypropyl trimethoxysilane (KHCS) as filler. Its chemical structure was characterized by Fourier transform infrared spectroscopy (FTIR). The dynamic thermodynamic properties, mechanical properties, flame retardant property, and fracture morphology were studied using dynamic mechanical analysis (DMA), a universal testing machine, a micro combustion calorimeter, and a scanning electron microscope (SEM), respectively. The effects of different contents of KHCS on various properties were discussed. The experimental result showed that the CS was bonded toKH560 by a covalent bond. The impact strength, tensile strength, and flexural strength of the composites were all improved compared with those of pure EP. When the content of KHCS was 15 wt%, the maximum impact strength of the composites was 3.31 kJ/m2, which was 1.43 times that of the pure EP. The p HRR and THR of MCSEC-20 were 512.44 W/g and 25.03 kJ/g, respectively, which were 40.71% and 27.76% lower than those of pure EP, when the content of KHCS was 20 wt%. Moreover, the mechanism of the curing composites was investigated.Keywords:epoxy resin;corn straw;composites;preparation;curing mechanism
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EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
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2014
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IF 3.432
Wenlin Xie,Shimin Xie,Ying Zhou,Xufu Tang,Jian Liu,Wenqian Yang,Minghua Qiu
A series of 5,6-disubstituted pyridine-2,3-dione-3-thiosemicarbazone derivatives(2a-2n) and 5,6-disubstituted pyridine-2,3-dione S-benzyl-3-thiosemicarbazones(3a-3g) were synthesized starting from 2,3-dihydroxypyridine via oxidation-Michael additions, condensations and nucleophilic substitutions. The structures of the compounds were established by IR, 1 H NMR, 13 C NMR, and HRMS . All newly synthesized compounds were screened for their anticancer activity against Breast cancer (MCF-7), Colon cancer (HCT-116) and hepatocellular cancer (BEL7402) cell lines. Bioassay results indicated that most of the prepared compounds exhibited cytotoxicity against various cancer cells in vitro. Some of the compounds exhibited promising antiproliferative activity , which were comparable to the positive control (5-fluorouracil). The structure–activity relationship was discussed.
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EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
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2020
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IF 5.573
Heping Zhu,Shilong Ying,Bingluo Zhou,Xiao Liang,Quan He,Ping Song,Xinyang Hu,Keqiang Shi,Mingteng Xiong,Hongchuan Jin,Yuanjiang Pan
Microtubules play a vital role in cell mitosis. Drugs targeting taxol or vinca binding site of tubulin have been proved an effective way to against cancer. However, drug resistance and cancer recurrence are inevitable, there is an urgent need to search for new microtubule-targeting agents (MTAs). In our study, a series of novel 2-aryl-3-sulfonamido-pyridines ( HoAns ) had been designed, synthesized, and evaluated for their antiproliferative activities in vitro and in vivo . Among them, compound HoAn32 exhibited the most potent activity with IC 50 values ranging from 0.170 to 1.193 μ M in a panel of cancer cell lines. Mechanism studies indicated that compound HoAn32 bound to the colchicine site of β-tubulin, resulting in colony formation inhibition, G2/M phase cell cycle arrest, cell apoptosis as well as increased the generation of ROS in both RKO and SW620 cells. In addition, compound HoAn32 showed potent anti-vascular activity in vitro . Furthermore, compound HoAn32 also exhibited outstanding antitumor activity in SW620 xenograft tumor models without observable toxic effects, which was more potent than that of ABT-751 . In conclusion, our findings suggest that compound HoAn32 may be a promising microtubule destabilizing agent and deserves for further development in cancer therapy.
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高影响因子
Catalysis Science & Technology
·
2023
·Vol. 13
·期 24
·7046-7058
·
IF 5
Wei Jin,Dekang Liu,Liyuan Zhang,Qian Sun,Yishan Wang,Enzhou Liu,Xiaoyun Hu,Hui Miao
Titanium dioxide (TiO2), a conventional n-type semiconductor, was widely used in photocatalysis, electrocatalysis and photoelectrocatalysis due to its good UV absorption and stable physical and chemical properties. However, its wide band gap and low oxygen reaction (OER) activity limited its application in photoelectrochemical (PEC) water splitting. In this work, we successfully constructed type-II Sb2(S,Se)3@TiO2 core–shell heterojunctions. Antimony sulfide selenide (Sb2(S,Se)3) was a quasi-one-dimensional light-absorbing material with an adjustable band gap (1.1–1.8 eV), which broadened the TiO2 light absorption range and effectively promoted the photogenerated carrier separation, transportation and utilization. Of particular note, novel Sb2(S,Se)3 nanospheres (NSPs) (ca. 69 nm) were in situ grown inside the tubes attributed to the unique space-confinement effect of TiO2 nanotubes (NTs). The IPCE value for Sb2(S,Se)3@TiO2 at 734 nm was 10.808% compared to 0.030% for TiO2. The separation efficiency and injection efficiency increased from 2.48% and 31.62% to 4.90% and 36.48%, respectively. The onset potential was moved negatively by 60 mV, and the maximum photocurrent density of 1.53 mA cm−2 at 1.23 V vs. RHE was 13.9 times higher than that of TiO2 (0.11 mA cm−2). This work provided a new idea for the application of TiO2 in the field of PEC water splitting.
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CERAMICS INTERNATIONAL
·
2023
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IF 5.2
Tingting Tang,Hua Huang,Chaoyang Li,Jian Shen
High performance and miniaturization are becoming increasingly important for practical microwave electronics and high-density communication systems. Here, LiNb 0.6 Ti 0.5- x (Zn 1/3 Nb 2/3 ) x O 3 (LNT- x ZN, x = 0, 2, 4, 6, 8 mol%) ceramics have been successfully prepared by a solid-phase reaction. The relationship between the physical structure, microscopic morphology, Raman spectroscopy , and microwave properties is revealed. The better microwave dielectric properties of the LNT-4ZN ceramic sintered at 1075 °C are ε r ∼59.9, Q× f ∼8261 GHz, and τ f ∼-7.4 ppm/°C. Using this ceramic, a miniaturized dielectric resonant antenna (DRA) is designed and demonstrated. The return loss, radiation efficiency, and realized gain of this device at the center frequency are −43.57 dB, 78.4 %, and 8.59 dB, respectively. The antenna, in addition to the good radiation performance, has a size advantage over similar devices (#Cylindrical sample, # R 3.1 mm × h 1.6 mm, @Square substrate, @ a = b = 18 mm × h 3.1 mm). This work exemplifies the great potential of LNT-4ZN materials in communication devices and promotes the application of microwave dielectric ceramics in antenna fields.
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JOURNAL OF ORGANIC CHEMISTRY
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2023
·
IF 3.6
Yuan Li,Jiamei Shen,Yawei Shen,Yanfeng Li,Kai Luo,Lei Wu
A novel and efficient tandem SN2 nucleophilic substitution/Dieckmann condensation reaction of α-iodomethyl phosphine oxide with methyl thiosalicylate derivatives has been developed by using NaOH as a base, which enables the expeditious synthesis of 2-phosphonyl-3-hydroxybenzo[b]thiophene derivatives in moderate to high yields under simple conditions. This research provides not only a convenient method for the functionalization of benzo[b]thiophenes at the 2-position and 3-position but also new organophosphorus molecules. Furthermore, several new phosphonyl-substituted benzo[b]thiophenes were obtained from the resultant 2-phosphonyl-3-hydroxybenzo[b]thiophenes.
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JOURNAL OF MOLECULAR STRUCTURE
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2023
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IF 3.8
JiongYa Tang,Wen Lu,Jiayi Hu,Yuqi Jia,Xuanyuxiang He,Lin Li,Shilong Yang,Yanqin Wang,Li Xu
The azobenzene coumarin derivatives 2 – 10 , in which the coumarin moiety is linked to substituted phenyl and pyridine groups via amide bonds, and a copper complex with compound 8 ( 8-CuCl 2 ) were designed and synthesized, and their anticancer activities were investigated. Compounds 2–10 and 8-CuCl 2 exhibited different degrees of anticancer activities against four cancer cell lines (HeLa, A549, MCF-7, and HepG-2). Among them, compound 6 exhibited stronger cytotoxicity (IC 50 = 0.51 ± 0.22 μM) against A549 cell lines and was less toxic to normal cells than Doxorubicin (DOX) (IC 50 = 1.18 ± 0.03 μM). Compound 8 exhibited stronger cytotoxicity against MCF-7 cell lines than DOX with an IC 50 value 48 times higher than DOX (IC 50 = 0.42 ± 0.23 μM for 8 ; IC 50 = 20.64 ± 3.67 μM for DOX). Meanwhile, compound 8 was much less toxic to normal human umbilical vein endothelial cells (HUVEC) cells than DOX with a 3000 times higher selectivity index (SI) (SI > 238.10 for 8 ; SI = 0.078 for DOX). The results of cell apoptosis and cycle assays showed that compound 6 induced apoptosis in A549 cells more considerably and blocked cells in the S phase, whereas compound 8 induced late apoptosis in MCF-7 cells and blocked cells in the S phase. Ultraviolet and fluorescence spectroscopy analyses revealed that compounds 6 and 8 bound to deoxyribonucleic acid (DNA) via intercalation. In summary, compound 8 was developed as a potential and effective cytotoxicity against MCF-7 cell lines worthy of further investigation.
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JOURNAL OF MEMBRANE SCIENCE
·
2023
·
IF 9.5
Zewen Xu,Zhongyi Ren,Haojiang Zhu,Hao Guo,Xinliang Liu,Ming Wang,Yingfei Hou
Layer-by-layer (LBL) assembly method, with the advantages of simple operation and strong universality, has attracted much attention recently. However, the weak molecule-to-molecule and molecule-to-ion interactions have limited its development. During this work, the molecule-to-solid interaction was adopted and a robust LBL coating was achieved by alternately depositing positively charged Fe(OH) 3 colloids and negatively charged sodium alginate molecules. The molecule-to-solid interaction could continuously regenerate under corrosive conditions due to water insolubility and the chemical stability of solids. When the electrostatic interaction was broken, the abundant chemically stable solids acted as anchors to immobilize the soluble molecules and maintain the stability of the LBL coating. The prepared superwettable membranes exhibited superior oil-in-water emulsion separation efficiency, anti-fouling properties, and chemical stability. Meanwhile, the separation performance survived one week's harsh environments of strong acid and alkali.
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ANTI-CANCER DRUGS
·
2023
·
IF 2.3
Wang Guangxin,Gao Xia,Sun Zhijun,He Tianyou,Huang Chaogang,Li Shouwei,Long Haocheng
Enhancing research indicatedthat circular RNA (circRNA) acted a critical part in cholangiocarcinoma (CHOL) development. This research aims to discover the role of circRNA SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin, subfamily a, member 5 ( SMARCA5 ) in CHOL bio-progression, which has been proved to be downregulated in CHOL tissues. In this study, quantitative reverse transcription polymerase chain reaction was used to reveal the level and linkage of circRNA SMARCA5 , miRNA-95-3p and TNF receptor-associated factor 3 gene ( TRAF3 ) in CHOL tissues and cancer cells. The target sites of circRNA SMARCA5 and miRNA-95-3p were forecast by Starbase, and Targetscan was conducted to forecast the potential linkage points of TRAF3 and miRNA-95-3p , and which is affirmed by double luciferase reporter assay. CCK-8 and flow cytometry assay was carried to indicate cell viability. And apoptosis-related protein was counted by caspase3 activity and Western blot assay. CircRNA SMARCA5 was downregulated in CHOL cell lines and cancer samples. Besides, over-expression of SMARCA5 inhibited cell growth and promoted apoptotic rate. Dual-luciferase reporter assays presented that miRNA-95-3p could link with circRNA SMARCA5 . Moreover, miRNA-95-3p was discovered highly expressed in CHOL. Interference of miRNA-95-3p repressed cell proliferation and raised the apoptosis. Importantly, TRAF3 was validated to be a downstream of miRNA-95-3p . Strengthen of miRNA-95-3p reversed the inhibitory impact of circRNA SMARCA5 -plasmid transfection, and the results of miRNA-95-3p inhibitor were reversed by si- TRAF3 . CircRNA SMARCA5 is involved in CHOL development by interosculating miRNA-95-3p / TRAF3 axis and may become a novel approach for treating CHOL.
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Surfaces and Interfaces
·
2023
·
IF 6.2
Hao Yang,Yi Liu,Hidetoshi Saitoh,Hao Chen,Hua Li
Biofouling is a stepwise accumulation process involving participation of various molecules and marine organisms. To tackle this complex threat, development of efficient long-term antifouling coatings yet keeps elusive. Here we report a newly constructed coating with both antifouling activity and low surface energy. The special surface structure formed by silica spheres and fluorosilanes offers the coating superhydrophobicity. Capsaicin-mimicking N-(4-hydroxy-3-methoxy-benzyl)-acrylamide (HMBA) powder is synthesized via the Friedel-Crafts reaction and is used as the antifoulant additives in the coating. By the synergistic effect of surface superhydrophobicity and the dispersed antifoulant, the multilayered HMBA-silica coating effectively inhibits the adhesion of alginate, bovine serum albumin and Chlorella , their adhesion rate on the HS-3 coating is 11.9 %, 8.7 % and 3.3 % respectively. The HS-3 coating kills 96.6 % gram-negative bacteria E. coli and 99.9 % gram-positive bacteria S. aureus. Furthermore, the HMBA-silica coating significantly mitigates by its superhydrophobicity the corrosion of artificial seawater, its corrosion current density has decreased by 4 orders of magnitude compared to bare 316 L plate. In a 90 days real seawater immersion experiment, the coating shows excellent anti-fouling and anti-corrosion properties. The fabrication route of the HMBA-silica coating using the synthesized broad-spectrum antifoulant HMBA and hydrophobic silica would give insight into developing new antifouling materials for marine applications.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Shaoxiong Liu,Xiao Li,Siwei Zhong,Wenjia Jiang,Ye Liu,Wenchong Ding,Hai Hu,Zhifeng Huang,Li Liu
NASICON-structured Ti-based polyanion compounds benefit from a stable structural framework, large ion channels, and fast ion mobility . However, the large radius of potassium and its poor electronic conductivity restrict its use in potassium-ion batteries. Herein, hierarchical mesoporous Mn 0.5 Ti 2 (PO 4 ) 3 @C microspheres have been successfully synthesized using a simple electrospraying method. These microspheres consist of Mn 0.5 Ti 2 (PO 4 ) 3 nanoparticles evenly embedded in three-dimensional mesoporous carbon microspheres. The hierarchical mesoporous micro/nanostructure facilitates the rapid insertion and extraction of K + , while the three-dimensional carbon microspheres matrix enhances electrical conductivity and prevents active materials from collapsing during cycling. So the hierarchical mesoporous Mn 0.5 Ti 2 (PO 4 ) 3 @C microspheres exhibit a high reversible discharge specific capacity (306 mA h g −1 at 20 mA g −1 ), a notable rate capability (123 mA h g −1 at 5000 mA g −1 ), and exceptional cycle performance (148 mA h g −1 at 500 mA g −1 after 1000 cycles). The results show that electrosprayed Mn 0.5 Ti 2 (PO 4 ) 3 @C microspheres are a promising anode for PIBs.
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