ANALYST
·
2022
·Vol. 147
·期 12
·2757-2763
·
IF 5.227
Xinying Zhang,Zhiwei Zhang,Chujing Yang,Ziheng Hu,Yingying Chen,Lingyan Feng
Signal amplification strategies increase the complexities of biosensors while improving the response signals. Herein, a novel electrochemical biosensor was developed based on a DNA hydrogel for sensitive analysis using microRNA-21 (miRNA-21) as a detection model. Poly C sequences combined with C–Ag(I)–C hydrogel formed a DNA hydrogel by the unique interaction between the cytosines and silver ions. Thus, with a three-way conjunction structure of DNA, this C–Ag(I)–C hydrogel was constructed as a novel biosensor for the detection of miRNAs. With the assistance of this hydrogel, numerous silver ions gathered around DNA strands, which would amplify the signal. Under these conditions, the silver ions produced distinct square wave voltammetry oxidation peak currents. This electrochemical biosensor we designed exhibited a great linear relationship for the logarithm of the concentration of miRNA-21 from 1 fM to 100 pM with a detection limit of 0.117 fM. Furthermore, our sensors were able to differentiate miRNA-21 from its homologous family with satisfactory responsiveness in the dilute bovine serum system.
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Advanced Healthcare Materials
·
2021
·Vol. 11
·期 2
·2101702
·
IF 9.933
Yuchen Zhang,Liqi Li,Yanan Li,Yang Fei,Chencheng Xue,Xuemei Yao,Youbo Zhao,Xuan Wang,Menghuan Li,Zhong Luo
Ferroptosis is an emerging antitumor option and has demonstrated unique advantages against many tumor indications. However, its efficacy is potentially hindered by the endogenous lipid peroxide-scavenging mechanisms and the reliance on acidic pH. Herein, a nanointegrated strategy based on clinically-safe components to synergistically remodel glutathione and lactate metabolism in tumor cells for enhanced ferroptosis therapy is developed. First ferrocene is conjugated on PEGylated polyamidoamine dendrimers via reactive oxygen species (ROS)-cleavable thioketal linkage, which would further self-assemble with the glutathione (GSH)-depleting agent diethyl maleate (DEM) and monocarboxylate transporter 4-inhibiting siRNA (siMCT4) to afford biostable nanoassemblies (siMCT4-PAMAM-PEG-TK-Fc@DEM). The nanoassemblies can be activated by the elevated ROS levels in tumor intracellular environment and readily release the incorporated therapeutic contents, afterward DEM can directly conjugate to GSH to disrupt the glutathione peroxidase 4 (GPX4)-mediated antioxidant defense, while siMCT4 can block the MCT4-mediated efflux of lactic acid and acidify the intracellular milieu, both of which can improve the ferrocene-catalyzed lipid peroxidation and induce pronounced ferroptotic damage. The siMCT4-PAMAM-PEG-TK-Fc@DEM nanoplatform demonstrates high ferroptosis-based antitumor potency and good biocompatibility in vitro and in vivo, which may offer new avenues for the development of more advanced antitumor therapeutics with improved translatability.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2020
·Vol. 21
·期 13
·4789
·
IF 4.556
Yiyu Wang,Chunqing Niu,Sisi Fan,Yuwei Li,Xiang Li,Yujun Dai,Jian Shi,Xinyu Wang
Photothermal therapy possesses great advantages for the treatment of drug-resistant tumors. Herein, Near Infrared (NIR)-triggered photothermal nanoparticles were developed through loading indocyanine green (ICG), a kind of NIR dye, into amino group-modified silica nanoparticles (SiO2-NH2NPs). SiO2-NH2NPs were prepared with immobilization of the amino groups into the framework of silica nanoparticles (SiO2NPs) by employing (3-aminopropyl)-triethoxysilane (APTES). Before and after the modification of the amino group, the particle sizes of SiO2NPs showed similar value, around 100 nm. ICG was further adsorbed into SiO2-NH2NPs by electrostatic attraction to enable SiO2-NH2@ICG NPs as a kind of photothermal agent. The loading rate of ICG to SiO2-NH2was greatly increased compared to unmodified SiO2, and the stability of ICG was also improved. Moreover, the SiO2-NH2@ICG NPs exhibited efficient photothermal effects due to ICG transforming laser power into local heat through the connected ICG, when NIR laser irradiation turned on for a couple of minutes. Finally, the in vitro antitumor efficacy of SiO2-NH2@ICG NPs was investigated by recording cell proliferation rate and further chronicled the apoptotic morphology evidence by a Calcein-AM/PI fluorescent staining assay, indicating the efficient photothermal targeted therapy for the HepG2 tumor cells.Keywords:mesoporous silica nanoparticles;indocyanine green;NIR-triggered;photothermal effect;tumor cells
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Collagen and Leather
·
2025
·Vol. 7
·期 1
·42
·
IF 9.2
Yi Xueling,Luo Duanyang,Liu Siqi,Chen Yanxu,Guo Jin,Yan Qingyu,Mao Hui
Antibiotic contamination has been a concerning environmental issue due to its potential threats to the development of drug-resistant bacteria and genes. Plant polyphenols were employed as biomolecule chelating ligands to graft onto collagen fibers, followed by the chelative immobilization of Fe ions. The as-obtained environmentally benign chelative biosorbent (CFBT-Fe) features a classic fibrous structure, excellent distribution and reservation of Fe ions, increased thermal stability, and inhibited swelling behavior owing to the introduction of bayberry tannin. An exceptional adsorption capacity of tetracycline on CFBT-Fe is exhibited across a broad pH range (3.0–10.0). Optimal adsorption capacity (31.30 mg/g) occurs at neutral pH 7.0, which is higher than many other similar biosorbents reported in the literature. The CFBT-Fe exhibits exceptional recyclability with sixth-cycle adsorption performance at 25.27 mg/g. The environmentally benign coordinative biosorbent is considered a promising candidate for the effective adsorptive removal of tetracycline from aquatic environments across a wide pH range. Graphical
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IEEE SENSORS JOURNAL
·
2025
·
IF 4.5
Chungang Wu,Xiaoming Chen,Ziang Bai,Qihang Ye,Bo Han,Maoqing Chen,Yingxuan Liu,Shuhua Ma,Yong Zhao
Microfluidics plays a significant role in various fields, including material synthesis, drug delivery, and disease diagnosis, and the flow velocity has a critical effect on the products. However, there is a lack of approaches for monitoring the flow velocity in the microchannel. To address this, we propose a novel fiber-optic flowmeter based on a flexible Fabry-Perot (F-P) cavity for online and non-invasive monitoring of products from the microfluidic devices. The flexible F-P cavity is constructed with a fiber-optic probe and gold-coated polydimethylsiloxane (PDMS) film, which can be easily integrated with versatile microfluidic devices through oxygen plasma bonding. Depending on the numerical investigation of film deformation under various film and fluid parameters, we experimentally characterize the sensitivity, reversibility, stability, and repeatability of this flowmeter. Then, the adaptability of the flowmeter is demonstrated by monitoring the flow velocity of seawater and sucrose solution. Moreover, we integrate this flowmeter with multiple microfluidic devices to verify the integration performance. Finally, we successfully achieve online and non-invasive monitoring of mixing efficiency, droplet diameter, and microalgae separation efficiency in the integrated microfluidic devices. This flowmeter can monitor the products from the microfluidic devices for disease diagnosis and drug delivery, with the advantages of good adaptability and easy integration.
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iScience
·
2025
·Vol. 29
·期 2
·
IF 4.1
Han-Ping Wang,Chen-Yu Xu,Wen Su,Yin-Song Wang,Yue Wang,Ming-Xin Cao
Periodontitis is a chronic inflammatory disease that leads to periodontal attachment loss and is a major cause of adult tooth loss. Current treatment of mechanical debridement is often ineffective in deep periodontal pockets, and antibiotic adjuncts carry the risk of bacterial resistance. Octyl gallate (OG), a natural plant-derived compound with antibacterial and antioxidant properties, is limited by poor water solubility and stability. This study aimed to develop a sustained-release ointment (OG-Ointment) using Pluronic F127 and ethylcellulose to enhance the delivery and efficacy of OG. The formulated OG-Ointment demonstrated excellent rheological properties, sustained drug release, and potent ABTS + radical scavenging. In vitro , it enhanced bacterial membrane permeability, inhibited P. gingivalis growth, and reduced ROS in macrophages. In a rat periodontitis model, OG-Ointment significantly reduced alveolar bone resorption, decreased ROS and inflammatory cytokines, and exhibited good biocompatibility. These findings suggest that OG-Ointment is a promising non-antibiotic adjunctive therapy for periodontitis, combining dual antioxidant and antibacterial functions with sustained release.
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LAB ON A CHIP
·
2017
·Vol. 17
·期 13
·2225-2234
·
IF 6.045
Binfeng Hu,Juanjuan Li,Lei Mou,Yong Liu,Jinqi Deng,Wei Qian,Jiashu Sun,Ruitao Cha,Xingyu Jiang
Microfluidic platforms capable of automated, rapid, sensitive, and quantitative detection of biomarkers from patient samples could make a major impact on clinical or point-of-care (POC) diagnosis. In this work, we realize an automated diagnostic platform composed of two main components: (1) a disposable, self-contained, and integrated microfluidic chip and (2) a portable instrument that carries out completely automated operations. To demonstrate its potential for real-world application, we use injection molding for mass fabrication of the main components of disposable microfluidic chips. The assembled three-layered chip with on-chip mechanical valves for fluid control consists of (1) a top silicone fluidic layer with embedded zigzag microchannels, reagent reservoirs and a negative pressure port, (2) a middle tinfoil layer with patterned antibody/antigen stripes, and (3) a bottom silicone substrate layer with waste reservoirs. The versatility of the microfluidics-based system is demonstrated by implementation of a chemiluminescence immunoassay for quantitative detection of C-reactive protein (CRP) and testosterone in real clinical samples. This lab-on-a-chip platform with features of quantitation, portability and automation provides a promising strategy for POC diagnosis.
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Applied Sciences-Basel
·
2025
·Vol. 15
·期 3
·1486
·
IF 2.5
Mengye Jia,Riya Jin,Yina Qiao,Yu Jiang,Jiaoqin Liu,Zengdi He,Zhaoguo Liu,Jingshuai Gao
2,2′,4,4′,6,6′-hexanitrostilbene (HNS) is a heat-resistant, low-sensitivity energetic material with widespread applications in pliable linear-shaped charges, high-temperature-resistant oil well perforating charges, and rocket propellants. However, the presence of highly toxic and mutagenic nitroaromatic compounds in HNS wastewater necessitates efficient and accurate detection methods. Unfortunately, the existing analytical methods for HNS detection are outdated and incompatible with modern equipment. This limits their application due to issues with detection range, accuracy, and cost. To address this gap, an improved method was developed using an Ultimate 3000 UHPLC system with methanol and water as the mobile phase and UV-Vis detection at 271 and 226 nm wavelengths. The results indicate that the optimal detection conditions are achieved with a methanol-to-water ratio of 70:30 and a flow rate of 0.5 mL/min, providing high accuracy and efficiency. Compared to traditional methods, this approach reduced the detection time by nearly 70%, with the shortest analysis time ranging from 6 to 6.5 min, significantly lowering the cost of HNS detection. The method demonstrated excellent linearity (R2> 0.9999) and high sensitivity within the concentration range of 0.50–150.00 mg/L, with precise and reliable results. This work provides both theoretical insights and experimental validation for the detection and analysis of HNS in wastewater.
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Advanced Materials Technologies
·
2023
·Vol. 8
·期 11
·2201967
·
IF 6.8
Jiping Zhao,Youlong Xu,Wenqiang Hou,Yizhuo Li,Xiangdong Ding
Although embedded capacitors have been applied and researched for many years, their large-scale application still faces challenges such as low capacitance density, high thickness, high cost, and incompatibility with integrated processes. In this work, a breakthrough has been made in the fabrication of ultra-thin tantalum (Ta) capacitors with ultra-high capacitance density that can be used for 3D packaging. The key to these excellent performances is the application of Ta foil with nano-porous structure to the anode of the capacitor. The Ta foil with high specific surface area (SSA) is successfully prepared by direct current pulse etching. At a current density of 15 mA cm −2 , a pulse frequency of 50 Hz, and a duty cycle of 30%, the SSA of Ta foil is increased by 76 times after etching in an electrolyte with 0.01 v% TOA for 30 min. Based on this, Ta capacitors with a form factor of less than 40 µm, showing a capacitance density of 750 nF mm −2 and a leakage current of less than 2.1 × 10 −7 A at 8 V is successfully fabricated. To the best of the authors’ knowledge, this is the highest capacitance density reported to date for the mentioned form factors.
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JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2013
·Vol. 160
·期 8
·H459
·
IF 2.588
Jing Zhang,Huihui Zhu,Meiling Wang,Wenchang Wang,Zhidong Chen
A novel and sensitive electrochemical method was proposed for the determination of Sunset yellow (SY) using an expanded graphite paste electrode (EGPE). The EGPE was prepared by mixing expanded graphite (EG) with solid paraffin. EG was characterized with scanning electron microscopy. Compared with flake graphite paste and carbon paste electrode, the EGPE showed a higher electrocatalytic activity toward the oxidation of SY. The EGPE exhibited wide linear response of 5.0 × 10−8−2.0 × 10−6 mol/L with a detection limit down to 5.0 nmol/L (S/N = 3) and good accuracy, precision, reproducibility. The proposed method was successfully applied in the determination of SY in jelly and fruit juice.
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Molecular Catalysis
·
2024
·
IF 3.9
YaoYao,Ming-Liang Shi,Xin-Yue Fan,Kun Li,Jing Li,Na Wang
Multi-component reactions (MCRs) enable the development of efficient and atom-economic methods for synthesizing pyrrole derivatives. However, the current MCRs methods for synthesizing pyrrole derivatives in the enzymatic process have been unexploited. Herein, we developed a one-pot three-component enzymatic promiscuous catalytic system with green, efficient, and atom-economic to construct pyrrole derivatives. In this system, amines and 1,3-dicarbonyl compounds formed enamines with subsequent Michael addition with nitroolefins catalyzed by trypsin. A series of pyrrole derivatives were successfully obtained with moderate to good yield (up to 92 %). In addition, molecular simulations were conducted to gain insight into the source of differing tolerance of aromatic amides and fatty amines and the intrinsic effect of solvents in this system. This enzymatic catalytic one-pot three-component system has excellent functional tolerance, simple operation, and application potential for industrial production demonstrated by the gram scale experiment.
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Nature Communications
·
2024
·Vol. 15
·期 1
·1-17
·
IF 14.7
Zhao Yiquan,Xue Le,Huang Zhiyi,Lei Zixian,Xie Shiyu,Cai Zhenzhen,Rao Xinran,Zheng Ze,Xiao Ning,Zhang Xiaoyu,Ma Fuying,Yu Hongbo,Xie Shangxian
Exploring microorganisms with downstream synthetic advantages in lignin valorization is an effective strategy to increase target product diversity and yield. This study ingeniously engineers the non-lignin-degrading bacterium Ralstonia eutropha H16 (also known as Cupriavidus necator H16) to convert lignin, a typically underutilized by-product of biorefinery, into valuable bioplastic polyhydroxybutyrate (PHB). The aromatic metabolism capacities of R. eutropha H16 for different lignin-derived aromatics (LDAs) are systematically characterized and complemented by integrating robust functional modules including O- demethylation, aromatic aldehyde metabolism and the mitigation of by-product inhibition. A pivotal discovery is the regulatory element PcaQ, which is highly responsive to the aromatic hub metabolite protocatechuic acid during lignin degradation. Based on the computer-aided design of PcaQ, we develop a hub metabolite-based autoregulation (HMA) system. This system can control the functional genes expression in response to heterologous LDAs and enhance metabolism efficiency. Multi-module genome integration and directed evolution further fortify the strain’s stability and lignin conversion capacities, leading to PHB production titer of 2.38 g/L using heterologous LDAs as sole carbon source. This work not only marks a leap in bioplastic production from lignin components but also provides a strategy to redesign the non-LDAs-degrading microbes for efficient lignin valorization.
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