TALANTA
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2024
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IF 5.6
Mingyue Tan,Yunqing Wang,Yunxia Ji,Rongchao Mei,Xizhen Zhao,Jie Song,Jinmao You,Lingxin Chen,Xiaoyan Wang
Nanoparticles (NPs) could be uptake orally and exposed to digestive tract through various sources such as particulate pollutant, nanomedicine and food additive. Inflammatory bowel disease (IBD), as a global disease, induced disruption of the intestinal mucosal barrier and thus altered in vivo distribution of NPs as a possible consequence. However, related information was relatively scarce. Herein, in vivo distribution of typical silica (SiO 2 ) and titania (TiO 2 ) NPs was investigated in healthy and IBD models at cell and animal levels via a surface-enhanced Raman scattering (SERS) tag labeling technique. The labeled NPs were composed of gold SERS tag core and SiO 2 (or TiO 2 ) shell, demonstrating sensitive and characteristic SERS signals ideal to trace the NPs in vivo . Cell SERS mapping revealed that protein corona from IBD intestinal fluid decreased uptake of NPs by lipopolysaccharide-induced RAW264.7 cells compared with normal intestinal fluid protein corona. SERS signal detection combined with inductively coupled plasma mass spectrometry (ICP-MS) analysis of mouse tissues (heart, liver, spleen, lung and kidney) indicated that both NPs tended to accumulate in lung specifically after oral administration for IBD mouse (6 out of 20 mice for SiO 2 and 4 out of 16 mice for TiO 2 were detected in lung). Comparatively, no NP signals were detected in all tissues from healthy mice. These findings suggested that there might be a greater risk associated with the oral uptake of NPs in IBD patients due to altered in vivo distribution of NPs.
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高影响因子
ANALYTICAL CHEMISTRY
·
2024
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IF 6.7
Cheng Qu,Yuchuang Geng,Zhongxiang Ding,Yuzhu Li,Hao Jiang,Mengke Su,Honglin Liu
A feedback inhibition effect of high autoinducer levels on metabolite secretion in Chromobacterium subtsugae (C. subtsugae) was evidenced by in situ spatiotemporal surface-enhanced Raman spectroscopy (SERS) profiling. The hierarchical hydrophobic plasmonic array in agar medium is structured by oil/water/oil (OL/W/OH) triphasic interfacial self-assembly. The hydrophobic layer acts as a “door curtain” to selectively permit adsorption of a quorum sensing (QS)-regulated fat-soluble metabolite, i.e., violacein (Vio), and significantly blocks nonspecific adsorption of water-soluble proteins, etc. The SERS profiling clearly evidences that the diffusion of N-hexanoyl-l-homoserine lactone (C6-HSL) in agar medium quickly triggers the initial synthesis of Vio in C. subtsugae CV026 but surprisingly inhibits the intrinsic synthesis of Vio in C. subtsugae ATCC31532. The latter negative response might be related to the VioS repressor of ATCC31532, which negatively controls violacein production without influencing the expression of the CviI/R QS system. Moreover, two sender–receiver systems are constructed by separately coculturing CV026 or ATCC31532 with Hafnia alvei H4 that secretes large amounts of C6-HSL. Expectedly, the cocultivation similarly triggers the initial synthesis of Vio in CV026 but seems to have a quite weak negative effect on the intrinsic synthesis in ATCC31532. In fact, the negative regulation in ATCC31532 might be affected by a diffusion-dependent concentration effect. The H4 growth and its secretion of C6-HSL are a slow and continuous process, thereby avoiding the gathering of local high concentrations. Overall, our study put forward an in situ SERS strategy as an alternative to traditional bioluminescent tools for highly sensitively analyzing the spatiotemporal communication and cooperation in live microbial colonies.
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ACS Sustainable Chemistry & Engineering
·
2024
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IF 7.1
Yingying Huang,Sihang Zhang,Shouxiang Jiang,Jiangtao Xu
Flexible surface-enhanced Raman spectroscopy (SERS) substrates have garnered more and more attention due to their noninvasive sample collection ability. However, the improvement of SERS performance gradually reaches a bottleneck with numerous studies on exploration of active materials. In this study, we constructed an Ag@TiO2@cotton SERS substrate with randomly distributed TiO2 nanocavities, which can generate a strong coupling cavity mode, thus dramatically enhancing SERS activities. The prepared SERS substrates demonstrate high detection sensitivity, with a limit of 10–10 M for MB solution, an impressive enhancement factor (EF) of 2.7 × 108, excellent signal reproducibility, and remarkable stability. Furthermore, the proposed material allows the sensitive detection of MG with a limit of 1.0 × 10–10 M through a wiping fish collection method. These flexible Ag@TiO2@cotton substrates expand the range of available SERS substrates, and their easy fabrication method and long-term sensitivity hold promise for the trace analysis of harmful substances.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
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IF 4.6
Xiangying Jin,Jinqiong Huang,Haixin Zhang,Meiqi Wang,Danping Xie,Wenchao Jia
Pesticide residues in agricultural products and environmental water pose significant threats to human health and ecosystems. The urgent need for rapid detection and degradation of complex samples containing multiple pesticides has driven advancements in detection methods. Surface-Enhanced Raman Spectroscopy (SERS), a powerful molecular detection technique, has attracted considerable attention in food safety, environmental monitoring, and biological analysis due to its unparalleled sensitivity, non-destructive characterization capabilities, and high specificity. In this study, we presented a SERS detection substrate based on filter paper incorporating Ag nanoparticles (AgNPs) and ZnO nanoparticles (ZnONPs). This innovative SERS substrate enables sensitive and recyclable detection of deltamethrin and atrazine pesticides. The detection limits for bromopropylate and atrazine were 38.87 μg/L and 39.35 μg/L, respectively. The method’s detection limits meet the groundwater quality standards for allowable limits in agricultural water use. Notably, the paper-based SERS substrate achieved rapid degradation of pollutants within 10 min under UV light exposure, demonstrating its efficacy. Even after five consecutive uses, the substrate maintained its capability to detect various pesticides within mixed samples. This study explores the application of paper-based SERS detection technology in the investigation of pesticide degradation mechanisms and kinetics, discussing the effects of environmental conditions such as pH and temperature on the experimental results. The experimental findings support the theoretical research. Additionally, density functional theory (DFT) models were used to study the hydrolysis evolution mechanisms of two pesticides under different pH conditions. Our study highlights that paper-based SERS detection technology is an effective method for studying the degradation mechanisms of pesticides. This innovative approach advances the understanding of pesticide degradation processes and opens avenues for further exploration.
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ANALYTICA CHIMICA ACTA
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2025
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IF 6
Xing Zhong,Hezheng Zhan,Xiao Wang,Min Zhang,Shoushan Wang,Meili Zhang,Faliang Cheng,Peng Liu
Background Surface-enhanced Raman spectroscopy (SERS) substrates have undergone extensive development over the years, yet the challenge of significantly enhancing their sensitivity persists. Most existing substrates face considerable difficulties in obtaining the strongest electromagnetic coupling to maximize SERS signal intensity, i.e., it is hard to achieve optimal structural parameters such as the gap width and particle size, and to fabricate surfaces that are free from contamination such as surfactants. Therefore, there is a pressing need for a substrate optimization approach that allows for in-situ monitoring and real-time dynamic adjustments to precisely achieve the ideal substrate characteristics for superior performance. Results In this study, a highly sensitive porous copper-gold (Cu–Au) SERS substrate was fabricated using the galvanic replacement reaction (GRR), coupled with in-situ SERS monitoring to optimize substrate preparation. The Cu–Au nanoparticles formed and grew on sacrificial templates while noble metal ions were reduced by the sacrificial metal during GRR. The substrate preparation process revealed that the optimal preparation time was 200 ± 20 s. The SERS performance with crystal violet (CV) as a probe molecule demonstrated the substrate's remarkable sensitivity with detecting concentrations as low as 10 −16 M, which surpasses most literature reports. The optimized SERS substrate was further tested for detecting malachite green (MG), yielding an ultra-high enhancement factor (EF) of 8.96 × 10 14 . The entire optimization process did not involve the addition of aggregation or surfactant agents, ensuring a clean substrate surface. Significance and novelty This study is a further proof of the significance of the in-situ optimization of SERS substrates via GRR which allows real-time adjustment of nanoparticle size and gap width to enhance sensitivity. This approach has enabled us to develop substrates with exceptional sensitivity and reproducibility. These significant contributions may open up new avenues for the facile fabrication of ultrasensitive SERS substrates.
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ACS Applied Materials & Interfaces
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2024
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IF 8.3
Peng-Cheng Guan,Qian-Jiao Qi,Yu-Qing Wang,Jia-Sheng Lin,Yue-Jiao Zhang,Jian-Feng Li
Traditional diagnostic methods, such as blood tests, are invasive and time-consuming, while sweat biomarkers offer a rapid physiological assessment. Surface-enhanced Raman spectroscopy (SERS) has garnered significant attention in sweat analysis because of its high sensitivity, label-free nature, and nondestructive properties. However, challenges related to substrate reproducibility and interference from the biological matrix persist with SERS. This study developed a novel ratio-based 3D hydrogel SERS chip, providing a noninvasive solution for real-time monitoring of pH and glucose levels in sweat. Encapsulating the probe molecule (4-MBN) in nanoscale gaps to form gold nanoflower-like nanotags with internal standards and integrating them into an agarose hydrogel to create a 3D flexible SERS substrate significantly enhances the reproducibility and stability of sweat analysis. Gap-Au nanopetals modified with probe molecules are uniformly dispersed throughout the porous hydrogel structure, facilitating the effective detection of the pH and glucose in sweat. The 3D hydrogel SERS chip demonstrates a strong linear relationship in pH and glucose detection, with a pH response range of 5.5–8.0 and a glucose detection range of 0.01–5 mM, with R2 values of 0.9973 and 0.9923, respectively. In actual sweat samples, the maximum error in pH detection accuracy is only 1.13%, with a lower glucose detection limit of 0.25 mM. This study suggests that the ratio-based 3D hydrogel SERS chip provides convenient, reliable, and rapid detection capabilities with substantial application potential for analyzing body fluid pH and glucose.
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NEW JOURNAL OF CHEMISTRY
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2025
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IF 2.5
Hao Zhong,Zhenyu Lin,Yongqiang Dong
For the first time, single-layer carbon dots (CDs) were used as capping agents to synthesize cuprous oxide nanosheets (Cu2O-NSs) as a high-performance surface enhanced Raman scattering (SERS) substrate, emphasizing their chemical enhancement mechanism. The Cu2O-NSs, synthesized using single-layer carbon dots as capping agents, exhibit uniform morphology and remarkable SERS activity, achieving an enhancement factor of 4.97 × 105 for crystal violet under 532 nm laser excitation. The SERS enhancement is primarily attributed to photoinduced charge transfer between the Cu2O-NSs and analyte molecules, as confirmed by energy-level alignment studies. The substrate demonstrates excellent signal homogeneity, batch-to-batch reproducibility, and long-term stability, with no significant signal attenuation over 60 days. These findings underscore the potential of Cu2O-NSs as a cost-effective and stable SERS substrate, with promising applications in chemical sensing and molecular detection.
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FOOD CONTROL
·
2024
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IF 5.6
Xinchen Wu,Limei Yin,Shipeng Gao,Ruiyun Zhou,Yang Zhang,Shanshan Xue,Heera Jayan,Hesham R. El-Seedi,Xiaobo Zou,Zhiming Guo
Patulin (PAT), a major contaminant in apples, poses a huge threat to human health as well as the economic sector. There is an urgent need to develop a sensitive, selective, and fast-responsive method to detect PAT in apples. However, one of the main challenges is overcoming the interferences of complex food matrices. In this study, we developed a highly sensitive competitive SERS sensor based on plasmonic nanoparticles modified by aptamers. The study utilized the formation of nanocomposites through aptamer-modified Au@Ag nanoparticles (NPs) and gold nano-stars (AuNSs) to induce high-intensity Raman signals from the SERS tag. Subsequently, in the presence of PAT, the nanocomposites underwent decomposition, evident through the significant decrease in SERS intensity. According to the standard curve established in this study, the detection limit was 0.0281 ng/mL. The competitive sensor was applied to spiked apple fruit and juice samples, indicating a recovery rate ranging from 91.98% to 102.94%. The excellent analytical performances and high sensitivity observed suggest the potential of the plasmonic nanocomposite sensing strategy in detecting PAT in real matrices.
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TALANTA
·
2025
·
IF 6.1
Rongjing Hu,Shilan Fu,Yongcong Zhou,Zhenyu Lin,Fengfu Fu,Yongqiang Dong
The current surface-enhanced Raman scattering (SERS) substrates typically feature a single energy level, posing challenges in coordinating electromagnetic enhancement (EM) and chemical enhancement (CM), thereby limiting the sensitive detection of numerous crucial target molecules. In this study, novel aggregated nanorings (a-NRs) hybridizing Ag, Au and AgCl are constructed as SERS substrates. On one hand, the obtained a-NRs exhibit robust localized surface plasmon resonance absorption, whose wavelength can be tuned to match three commonly used laser wavelengths (532, 633 and 785 nm) to gain strong EM effect. On the other hand, these materials possess the Fermi levels of Au nanoparticles and Au/Ag alloy, in addition to the valence band and conduction band of AgCl. The abundant energy levels of the obtained a-NRs facilitate increased charge transfer opportunities for molecules, leading to a strong CM effect. Therefore, the obtained a-NRs show ultra-high SERS sensitivity towards numerous molecules. Moreover, the unique chemical composition makes the obtained a-NRs have good long-term stability in terms of SERS activity. Besides providing high-performance SERS substrates, the valuable experience for coordinating EM and CM to construct highly active SERS substrate demonstrated in this work are expected to significantly advance the application of SERS.
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高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Yifei He,Jinxiang Wei,Lili Zhang,Yu Xia,Zhouping Wang,Junsong Yang
In this study, a dual-mode SERS-fluorescent aptasensor based on a DNA walker was developed for Shigella flexneri ( S . flexneri ). Fe@SiO2@QDs were connected with H1 and the walking chain (hybrid strands of aptamer and cDNA) to fabricate fluorescent probes, and Ag R6G @SiO 2 were connected with H2 to fabricate SERS tags. Aptamer (Kd = 42.6 ± 3.16 nM) selective binding of S . flexneri , left the walking chain. The Zn 2+ -specific DNAzyme in the cDNA was exposed thus driving the DNA walker. The sequence on H1 containing BHQ3 was cleaved from fluorescent probes to restore the fluorescence. The remaining H1 sequence could bind to the H2 to generate Raman signals. The detection limit of the fluorescence signal was 11 CFU/mL, and the Raman signal was 3 CFU/mL. In addition, the aptasensor was successfully applied to the detection of actual samples with recovery ranges of 98.35–107.27% and 97.57–105.91% for the fluorescence and SERS methods, respectively.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2024
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IF 9.4
Xiong Zhao,Chenyi Cui,Li Ma,Zihan Ding,Junsheng Hou,Yaxuan Xiao,Biwu Liu,Baojin Qi,Jinhua Zhang,Xinlan Lu,Jinjia Wei,Satoshi Watanabe,Nanjing Hao
SERS measurements for monitoring bactericides in dairy products are highly desired for food safety problems. However, the complicated preparation process of SERS substrates greatly impedes the promotion of SERS . Here, we propose acoustofluidic one-step synthesis of Ag nanoparticles on paper substrates for SERS detection. Our method is economical, fast, simple, and eco-friendly. We adopted laser cutting to cut out appropriate paper shapes, and aldehydes were simultaneously produced at the cutting edge in the pyrolysis of cellulose by laser which were leveraged as the reducing reagent. In the synthesis, only 5 μL of Ag precursor was added to complete the reaction, and no reducing agent was used. Our recently developed acoustofluidic device was employed to intensely mix Ag + ions and aldehydes and spread the reduced Ag nanoparticles over the substrate. The SERS substrate was fabricated in 1 step and 3 min. The standard R6G solution measurement demonstrated the excellent signal and prominent uniformity of the fabricated SERS substrates. SERS detection of the safe concentration of three bactericides , including tetracycline hydrochloride , thiabendazole , and malachite green, from food samples can be achieved using fabricated substrates. We take the least cost, time, reagents, and steps to fabricate the SERS substrate with satisfying performance. Our work has an extraodinary meaning for the green preparation and large-scale application of SERS.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Xueying Gao,Ying Liu,Jinxiang Wei,Zhouping Wang,Xiaoyuan Ma
Aflatoxin B 1 (AFB 1 ) is a virulent metabolite secreted by Aspergillus fungi, impacting crop quality and posing health risks to human. Herein, a dual-mode Raman/fluorescence aptasensor was constructed to detect AFB 1 . The aptasensor was assembled by gold nanoparticles (AuNPs) and magnetic nanoparticles (MNPs), while the surface-enhanced Raman scattering (SERS) and fluorescence resonance energy transfer (FRET) effects were both realized. AuNPs were modified with the Raman signal molecule 4-MBA and the complementary chain of AFB 1 aptamer (cDNA). MNPs were modified with the fluorescence signal molecule Cy5 and the AFB 1 aptamer (AFB 1 apt). Through base pairing, AuNPs aggregated on the surface of MNPs, forming a satellite-like nanocomposite, boosting SERS signal via increased “hot spots” but reducing fluorescence signal due to the proximity of AuNPs to Cy5. Upon exposure to AFB 1 , AFB 1 apt specifically bound to AFB 1 , causing AuNPs detachment from MNPs, weakening the SERS signal while restoring the fluorescence signal. AFB 1 concentration displayed a good linear relationship with SERS/fluorescence signal in the range of 0.01 ng/mL–100 ng/mL, with a detection limit as low as 5.81 pg/mL. The use of aptamer assured the high selectivity toward AFB 1 . Furthermore, the spiked recovery in peanut samples ranged from 91.4 % to 95.6 %, indicating the applicability of real sample detection. Compared to single-signal sensor, this dual-signal sensor exhibited enhanced accuracy, robust anti-interference capability, and increased flexibility, promising for toxin detection in food safety applications.
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