BIOELECTROCHEMISTRY
·
2026
·
IF 4.9
Lan Jiang,Lina Zou,Jing Dong,Ping Zhang,Jian Zhou,Hao Jiang,Huanyu Ren,Huiru Niu,Hao Liao,Xiaojing Zhang,Shanshan An,Fei Ren,Xiuhong Ge,Feiyan Yang,Lang Cheng,Hongzhi Pan,Shengzhong Rong,Hongkun Ma
Non-coding RNAs are critical tumor biomarkers, rendering innovations in their detection technologies highly significant. To overcome the limitations of conventional single-signal electrochemical biosensors (susceptibility to interference, insufficient stability), this study developed a novel ratiometric electrochemical DNA biosensor with dual-signal amplification. Its core novelty is the rational integration of two synergistic nanomaterials: Ferrocene-functionalized MgAl-layered double hydroxide (Fc@MgAl-LDH, as stable immobilization matrix and internal reference signal source) and AuNPs@Fc@MgAl-LDH coupled with MB-cDNA@AuNPs, which construct a cascaded signal amplification pathway. The sensing mechanism involves two-stage amplification: Primary by high-loading-capacity AuNPs@Fc@MgAl-LDH, secondary by MB-cDNA@AuNPs hybridizing with electrode-immobilized microRNA-21 (miRNA-21). The ratiometric signal ( I MB / I Fc ) enables built-in correction, boosting detection accuracy and reproducibility. After systematic optimization, the sensor has a wide linear range (1.0 fM–0.1 nM) and an ultra-low LOD of 0.757 fM, eliminating RNA extraction/enzymatic amplification to simplify operation. Serum validation shows high accuracy (95.3%–101.4% recovery), indicating strong clinical diagnostic application potential.
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高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2022
·Vol. 38
·期 6
·1453-1460
·
IF 2.726
Yi Jiaqi,Li Xiaoshuang,Cui Di,Han Lixia,Jiang Wei,Zhang Renguo,Niu Na,Chen Ligang
A novel fluorescent probe has been constructed based on fluorescence resonance energy transfer(FRET) between upconversion nanomaterials(UCNPs) NaYF 4 :Yb,Er and gold nanoparticles(AuNPs). The fluorescent “off-on” switching was formed for the detection of thiamphenicol(TAP) in egg samples. The fluorescence of UCNPs can be quenched to a certain degree by AuNPs. After adding TAP, the AuNPs generated aggregation and the fluorescence of UCNPs was recovered. The synthesized amination UCNPs and AuNPs were characterized by Fourier transform infrared spectroscopy(FTIR), UV-Vis, X-ray diffraction(XRD), energy dispersive spectrometer(EDS), and transmission electron microscope(TEM) techniques for observation and confirmation. As a model target, the detection of TAP has two linear ranges in the buffer solution within 0.01–0.1 µmol/L and 0.1–1 µmol/L using this fluorescent probe. The detection limit was obtained to be 0.003 µmol/L( S/N =3), which is favorable for trace analysis. The recovery of TAP from 98.2% to 105.3% was obtained, and the relative standard deviation(RSD) was from 2.5% to 4.3%. Furthermore, the method established in this study based on the UCNPs auto-low background fluorescence has high selectivity and strong ability to eliminate interference, which is beneficial to analyzing complex samples.
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BIORESOURCE TECHNOLOGY
·
2024
·
IF 9.7
Zhang Kai,Xin Jiaying,Lu Xuechun
This study established a Candida rugosa lipase (CRL) system to catalyze triolein and ethyl ferulate interesterification. The products were identified, and the binding mode between the substrates and CRL was predicted through molecular docking. Three methods for preparing CRL-AuNPs were proposed and characterized. It was found that the addition of 40 mL of 15 nm gold nanoparticles increased the CRL activity from 3.05 U/mg to 4.75 U/mg, but the hybridization efficiency was only 32.7 %. By using 4 mL of 0.1 mg/mL chloroauric acid, the hybridization efficiency was improved to 50.7 %, but the enzyme activity was sharply decreased. However, when the molar ratio of Mb to HAuCl 4 was 0.2, the hybridization efficiency increased to 71.8 %, and the CRL activity was also enhanced to 5.98 U/mg. Under optimal conditions, the enzyme activity of CRL-AuNPs③ was maintained at 95 % after 6 repetitions and 85.6 % after 30 days at room temperature.
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高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Mohamed Bahri,Guoguang Rong,Xixi Song,Peiwu Qin,Mohamad Sawan
Signal amplification has become a paramount strategy in biosensing, offering substantial improvements in sensitivity and detection limits for analytical platforms. However, conventional methods often rely on separate amplification stages and intricate chemical functionalization, leading to inefficiencies and greater complexity in biosensing workflows. In this article, we introduce a novel dual-purpose application of AuNPs@CuMOF for both surface functionalization and signal amplification, enabling electrochemical biosensing of neuron-specific enolase (NSE), a key biological marker for neuronal disorders and small cell lung cancer. The proposed biosensing strategy implies immobilizing the biotinylated NSE antibody onto the AuNPs@CuMOF-functionalized surface of a screen-printed carbon electrode (SPCE), alongside a thiolated NSE aptamer conjugated to AuNPs@CuMOF for signal amplification. Following this approach, the proposed immunosensor showcased a detection limit (LOD) down to 0.02 pg/mL within the span of 0.1 pg/mL to 200 ng/mL, outperforming or matching previously reported NSE biosensors. Besides, the suggested NSE biosensor reliably identified NSE in spiked human serum samples, showing both strong selectivity and sensitivity, with performance metrics on par with those of standard ELISA tests, with the relative error ranging from 12.03% to 5.63%. By leveraging the combined advantages of CuMOF’s large surface area, versatility, and stability, the proposed method simplifies the biosensing workflow while enhancing sensitivity through intrinsic amplification, resulting in a robust and efficient platform for the detection of biomarkers. We highlight in this work the transformative potential of multifunctional nanomaterials in advancing electrochemical biosensing technologies, wihch represents a significant milestone in tumor diagnosis in clinical settings.
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高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2021
·
IF 5.455
Sisi Su,Lanlan Lin,Haiyang Li,Xinyu Wen,Rui Yan,Caihong Tao
Photothermal material was one of the hot topics in nanomedicine in recent years, which had aroused widespread attention of scholars. In this paper, a kind of core-shell drug nanocarriers with good photothermal properties were prepared, and its photothermal therapeutic effect on tumor cells in vitro was studied. First, fluorescein isothiocyanate (FITC) was encapsulated in silica nanoparticles to form silica fluorescent (F-SiO 2 ) nanoparticles. Then mesoporous dopamine (MPDA) layer were built on the F-SiO 2 nanoparticles, the core-shell nanoparticles (F-SiO 2 @MPDA) with mesoporous shell structure were obtained. Finally, Au nanoparticles (AuNPs) were wrapped on the surface of the layer of MPDA shell by the citrate reduction method, and the F-SiO 2 @MPDA-AuNPs composite nanoparticles were formed. Then, the fluorescence property, adsorption performance, sustained release property, photothermal property and cytotoxicity of the F-SiO 2 @MPDA-AuNPs nanoparticles were researched. It was found that F-SiO 2 @MPDA-AuNPs nanoparticles had good fluorescence performance, high adsorption capacity (DOX, 111.275 mg/g) and pH responsive release performance. When pH = 5.2, the DOX release rate was 39.5% within 7 h under the action of near-infrared light (NIR). In addition, it also had a high photothermal conversion rate (36.95%), and the DOX loaded F-SiO 2 @MPDA-AuNPs nanoparticles had obvious inhibitory effect on tumor cells in vitro. As a kind of mesoporous drug nanocarriers with photothermal properties, it has potential application value.
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MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Huiting Xi,Xingguang Chen,Yao Cao,Junjun Xu,Cuizhu Ye,Danwen Deng,Jinsheng Zhang,Ganhui Huang
Formaldehyde is a poison around us. We described an electrochemical sensor based on polypyrrole(PPy)-reduced gold for the sensitivity detection of formaldehyde. The pyrrole was modified on glassy carbon electrode by potentiostatic method to obtain PPy/GCE. The gold nanoparticles (AuNPs) were decorated to the PPy/GCE after the treatment of the polypyrrole by overpotential. Thus, a AuNPs-polypyrrole composite modified glassy carbon electrode (AuNPs /PPy/GCE) was obtained. The material was characterized by SEM, EDX and XPS. The optimization of modification conditions for polypyrrole and AuNPs was determined. The PPy/GCE modified by AuNPs has high conductivity and excellent catalytic activity because of its large current response. The formaldehyde detection sensor based on AuNPs /PPy/GCE was established, and the quantitative determination of formaldehyde was carried out by differential pulse voltammetry(DPV). The electrochemical sensor has high selectivity, reproducibility and stability for formaldehyde detection. The sensor can be used to detect formaldehyde in pure water with detection line of 20 μM. The sensor can also be used to detect formaldehyde in milk with detection line of 0.4 mM.
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高影响因子
Small
·
2020
·Vol. 16
·期 39
·2001416
·
IF 11.459
Jing Bao,Xiaopei Qiu,Huisi Yang,Wenqiang Lu,Mei Yang,Wei Gu,Lixiang Wu,Danqun Huo,Yang Luo,Changjun Hou
Real-time quantitative monitoring of miRNAs plays an essential role in diagnosis and therapeutics. Herein, a DSN-coupled graphene nanoarray/gold nanoparticles (GNAs/AuNPs) carbon paper (CP) electrode for the dynamic, sensitive, and real-time analysis of miRNAs is reported. GNAs are vertically grown on the conductive CP by radio frequency plasma enhanced chemical vapor deposition, and AuNPs are electrodeposited on CP/GNAs to build a 3D ultrasensitive sensing interface with large specific surface area, good conductivity and biocompatibility. The dynamic quantitative monitoring of microRNA-21 (miR-21) is realized by cyclic voltammetry with a series of different concentrations within 16 min, and this 3D GNAs/AuNPs DNA-circuit strip shows good performance for the simultaneous detection of miR-21 and miR-155, and the detection limits are as low as 21.4 and 30.3 am, respectively. Moreover, comparable detection results are achieved for clinical samples between the proposed sensor and qRT-PCR, suggesting the reliability of the constructed sensor. This ultrasensitive sensing and disposable DNA-circuit strip with 3D structure can efficiently shorten the diffusion distance between reactive biomolecules and the sensing interface, enhance the hybridization of probes and improve the sensitivity of the biosensor, holding great promise for the rapid, quantitative and dynamic monitoring of multiple low concentrations of biomolecules in point-of-care clinical analysis.
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International Journal of Electrochemical Science
·
2023
·
IF 1.5
Zejun Zhang,Yunxiu Sun,Baoli Wang,Yijing Ai,Fan Shi,Yucen Yao,Xueliang Niu,Wei Sun
A novel sensing system was constructed by using gold nanoparticles (AuNPs) and MXene-Ti 3 C 2 T x nanocomposite decorated glassy carbon electrode (GCE) for dopamine (DA) determination. The AuNPs/MXene nanocomposite was prepared via a drop coating method, which could appreciably enlarge the electrochemically active surface area of as-prepared sensor and promote electron transfer with the enhanced electrochemical signals. Applying AuNPs/MXene/GCE in the optimum conditions, differential pulse voltammetric (DPV) current responses vs. DA concentrations are linear in the wider range of 2.0-500.0 μmol/L along with low detection limit of 0.67 μmol/L (S/N=3). Moreover, the proposed AuNPs/MXene modified GCE exhibited excellent stability, selectivity and reproducibility. Finally, this sensor had been utilized to detect DA successfully in DA hydrochloride injection with satisfactory recoveries, providing a promising application for further sensing analysis.
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高影响因子
ANALYST
·
2013
·Vol. 138
·期 18
·5365-5370
·
IF 3.969
Zhao Zhang,Liqiang Luo,Limei Zhu,Yaping Ding,Dongmei Deng,Zhenxin Wang
A novel protocol of electrochemical thrombin aptasensor based on a goldnanoparticles/thionine–graphene (AuNPs/Th–G) nanocomposite modified glassy carbon electrode was presented. Graphene was non-covalently functionalized by thionine via π-stacking interaction, and then AuNPs were electrodeposited onto the Th–G surface. The morphology and conductivity of the AuNPs/Th–G nanocomposite were characterized by scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. The ethanethiol-substituted oligonucleotide probe was immobilized onto the surface of the AuNPs/Th–G nanocomposite to prepare an electrochemical biosensing platform. The aptamer–thrombin reaction was monitored by differential pulse voltammetry. Under optimum conditions, the proposed biosensor exhibits high sensitivity and a low detection limit for thrombin determination. The thrombin could be quantified in a wide range of 0.5 to 40 nM and with a low detection limit of 0.093 nM (S/N = 3).
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ACS Applied Nano Materials
·
2024
·
IF 5.3
Mohamed Bahri,Zina Fredj,Peiwu Qin,Mohamad Sawan
Detecting preinvasive tumors before the onset of advanced clinical symptoms represents a critical advancement in improving the efficacy of early medical interventions and reducing cancer-related mortality rates. Immunosensors have emerged as the preferred choice for analyzing oncomarkers and meeting the ever-increasing demands of medical diagnostics. Therefore, the quest for the development of highly specific and sensitive immunosensors remains most sought-after to open new avenues for personalized healthcare and medical monitoring. Here, we report amplification-free and nonlabeled DNA framework-coupled AuNPs@CuMOF for ultrasensitive and highly specific electrochemical detection of carcinoembryonic antigen (CEA). Upon the CEA concentration variation from 0.0001 to 200 ng mL–1, the proposed biosensor led to a limit of detection (LOD) of 0.25 pg mL–1, surpassing most previously published CEA biosensors that rely on amplification-free methods and aligns with those based on amplification techniques. Furthermore, the suggested CEA immunosensor demonstrated auspiciously high specificity and sensitivity in detecting CEA in human serum comparable to the commercial ELISA within a relative error falling between 6.25% and 2.26%. The proposed analytical approach, hinging on the synergy effect of the CuMOF’s large surface area and the mechanical rigidity of the three-dimensional tetrahedron DNA structured probes (3D TDNA), obviously broadens the potential of immunosensors, offering higher sensitivity and lower LOD. Therefore, this work is not only an expansion of CuMOF exploitation in electrochemical biosensing but also a major milestone in detecting diverse biomarkers in clinical settings for tumor diagnosis.
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高影响因子
Frontiers in Bioengineering and Biotechnology
·
2024
·
IF 4.3
Chen Xiaohua,Liu Hengyu,Zhang Qianqian,Chen Xuehua,Wang Lihui,Yu Yanling,Hao Yuanping
Acidic bacterial biofilms-associated enamel white spot lesions (WSLs) are one of the hallmarks of early caries, causing demineralization and decomposition of dental hard tissues. Therefore, to effectively prevent and treat WSLs, it is important to inhibit the activity of cariogenic bacteria while promoting the remineralization of demineralized enamel. Amorphous calcium phosphate (ACP) favors hard tissue remineralization due to its biological activity and ability to release large amounts of Ca2+ and PO43-. However, ACP-based biomineralization technology is not effective due to its lack of antimicrobial properties. Here, carboxymethyl chitosan (CMCS) was employed as a reducing agent and stabilizer, and dual-functional nanohybrids CMCS/AuNPs/ACP with biofilm resistance and mineralization properties were successfully synthesized. The addition of AuNPs enhances the antimicrobial activity and participates in regulating the formation of hydroxyapatite (HAp). The nanohybrids exhibited significant destructive effects against cariogenic bacteria and their biofilms and showed bactericidal activity under bacteria-induced acidic conditions. More importantly, this nanohybrids showed superior results in promoting the remineralization of demineralized enamel, compared to fluoride and CMCS/ACP in vitro. The CMCS/AuNPs/ACP nanohybrids not only reverse the cariogenic microenvironment at the microbial level, but also promote self-repairing of enamel WSLs regarding the microstructure. The present work offers a theoretical and experimental basis for using the CMCS/AuNPs/ACP nanohybrids as a potential dual-functional agent for the clinical treatment of enamel WSLs.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xinyi Zhou,Siyu Fang,Luoyan Lin,Hao Feng,Tianyu Li,Lu Fang
Heparin is vital in treating thrombotic disorders; it works well as an anticoagulant coating on biomedical devices. Due to its pharmacological nature, excessive heparin can lead to thrombocytopenia, hemorrhage, and hyperkalemia, while too little may cause thrombi. Precise measurement of heparin concentration is essential for clinical practice; therefore, this study developed a heparin sensor utilizing Poly(3,4-ethylenedioxythiophene): Poly(styrenesulfonate) PEDOT:PSS/gold nanoparticle (AuNPs) nanocomposites. Heparin quantification is achieved via the specific protamine-heparin interaction, with protamine immobilized on the electrode surface. We systematically optimized the deposition conditions of PEDOT:PSS/AuNPs and the concentration of protamine and its incubation time. Following this optimization, the sensor's performance was evaluated using cyclic voltammetry (CV) in a 0.5 mM [Fe(CN)₆] 3−/4- solution over a voltage range spanning from −0.3 V to 0.7 V. The GCE/PEDOT:PSS/AuNPs/PRTM sensor achieved a heparin sensitivity of 8.113 μA·mM −1 ·cm −2 , a low detection limit of 0.05 μM, and a wide linear detection range of 0-35 μM. Under simulated physiological conditions (2.5 % human serum - containing buffer), the system exhibited a linear response to heparin (0–35 μM) and achieved recovery rates of 95.2 %–101.5 %, confirming its anti-interference capability in biological environments. The sensor demonstrated rapid response, high selectivity, wide linear range, and robust reliability, thus enabling its potential as a POCT device for real-time heparin monitoring in blood.
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