CHEMICAL ENGINEERING RESEARCH & DESIGN
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2026
·
IF 3.9
Liang Zhang,Haifeng Lu,Xiaolei Guo,Haifeng Liu
This study addresses the challenges of micro-dosing and mixing in the continuous manufacturing of solid pharmaceutical formulations by developing a novel continuous feeding-mixing system based on external vibration excitation. The innovative design of a hopper structure with adjustable outlet area distribution demonstrated the potential for achieving precise mixing control of an active pharmaceutical ingredient (API) and an excipient across varying ratios. Experiments first validated the promotion of viscous particle flow by vertical vibration, achieving stable feeding within the range of 0.6–15 mg/s. Building on this, precise control of mixing ratios was enabled by designing different outlet area ratios. In mixing performance evaluation, online X-ray fluorescence spectroscopy confirmed the system achieves high-uniformity mixing with a coefficient of variation below 10 %. The study further revealed that optimal mixing stability occurs when Γ < 5, whereas excessive vibration degrades mixing quality.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Mengyue Gao,Zhiheng Li,Mengjie Liu,Ting He,Xiaolu Su,Yueting Zhao,Zhiqiao Zou,Yanxing Yao,Fengmei Jin,Zhihao Li,Lihua Wang
A dual-metal-site MOF nanozyme exhibits enhanced oxidoreductases-like activity. • DMS MOF nanozyme-based sensor shows high sensitivity in Cr(VI) detection. • The sensor shows excellent performance in dairy product samples. A dual-metal-site metal-organic framework (DMS MOF) nanozyme was developed for highly sensitive colorimetric detection of Cr(VI) in dairy products. DMS MOF nanozyme integrates trivalent iron nodes and iron-porphyrin ligands to mimic the multi-center natural oxidoreductases, with adjacent dual-metal centers synergistically enhancing catalytic activity. Steady-state kinetic analyses and Zeta potential investigations demonstrate that the DMS MOF exhibits significantly lower Michaelis-Menten constants and enhanced substrate affinity than single-metal-site MOFs. The proposed method based on DMS MOF nanozyme enables highly sensitive colorimetric sensing of Cr(VI) within merely 5 min, achieving a broad linear range from 0.50 ppb to 5.0 ppm and a limit of detection (LOD) of 0.36 ppb. Furthermore, the developed strategy has been validated in complex dairy products including pure milk, yogurt, and milk powder, yielding recoveries of 84.0–110% with RSD ≤2.10%. Thus, engineered DMS-MOF nanozymes with enhanced activity provide a cost-effective, reliable approach for heavy-metal detection in foods. Download: Download high-res image (173KB) Download: Download full-size image
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Separation Science Plus
·
2026
·Vol. 9
·期 3
·e70209
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IF 2.7
Lianfeng Liu,Turghun Muhammad,Jia Zhao,Feiyu Zhan,Yan Zhang
To address the poor reproducibility associated with manual acceptor phase collection in two-phase hollow-fiber liquid-phase microextraction, we introduce a novel centrifugal collection technique for the first time. Thiamine, selected as a model analyte, was oxidized into fluorescent thiochrome and extracted via hollow-fiber liquid-phase microextraction. Following extraction, centrifugation at 7000 rpm quantitatively transferred 15.7 ± 0.4 µL (relative standard deviation [RSD] 2.55%) of the acceptor phase, accounting for over 80% recovery, from the hollow fiber into a glass capillary for direct fluorescence detection. This procedure enhanced collection efficiency nearly 7-fold and reduced RSD to 2.55%, compared with 2.3 ± 0.5 µL and 21.74% for the conventional syringe-based method. Optimal extraction conditions were established as follows: n -heptanol as the acceptor phase, a 20 min extraction time, 400 rpm orbital shaking, 15% NaCl, and 8 min for acceptor phase loading. Under these optimal conditions, a good linear relationship was established for thiamine across the concentration range of 0.05–3.0 µg/mL ( R 2 = 0.9996), with recoveries exceeding 91.0% and a limit of detection (LOD) of 1.112 × 10 −2 µg/mL. The intra-day and inter-day RSD ( n = 7) were below 1.7%. The proposed centrifugal collection method is simple, highly reproducible, and efficient, offering a generic strategy to enhance reliability and throughput for the hollow-fiber liquid-phase microextraction technique.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2026
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IF 4.8
Junnan Wang,Lei Hou,Yusong Zhang,Lei Yang,Wei Shi
In recent years, although quantitative, nondestructive terahertz (THz) testing has developed rapidly, a broadband and fast approach for material quantification is still lacking. In this paper, a Rydberg-atom THz detector is applied to measure the concentration of α -lactose monohydrate ( α -LM). Specifically, when cesium atoms are excited to the 40D 5 / 2 state by two lasers, the probe transmission exhibits an electromagnetically induced transparency (EIT) signal, and then splits into an Autler–Townes doublet in the presence of a THz field, with the splitting interval proportional to the THz field strength. Meanwhile, α -LM attenuates the THz field incident on the cesium atom vapor, which reduces the AT splitting, therefore, the sample concentration can be inferred by the AT splitting interval and concentration relationship. Moreover, the same samples were measured by a conventional THz time-domain spectroscopy (THz-TDS) system for comparison. While the THz-TDS system failed to establish a reliable calibration at non-resonant frequencies, the Rydberg-atom detector achieved excellent agreement with the Beer–Lambert law across a wide range of concentrations, even at a non-resonant frequency of 108.9 GHz. Consequently, this approach has no requirement for the sample ′ s characteristic absorption peaks and the system ′ s bandwidth. The proposed method proves Rydberg-atom sensor is a powerful and versatile tool for quantitative THz spectroscopy, and pave a way for non-destructive testing and material analysis.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Haiqing Wang,Wei Shi,Lei Hou,Chunhui Li,Zhiquan Wang,Lei Yang,Juncheng Cao
The rapid and accurate identification of the types and contents of early pathological markers by THz technology are of particular importance for the prevention and treatment of major diseases. Nevertheless, these markers usually contain interference from water and other non-target molecules, resulting in low signal-to-noise ratio (SNR) and making identification and quantitative analysis difficult. Here, based on THz spectroscopy from a high-power THz source radiated by LiNbO 3 , we perform quantitative and real-time THz detection of mixtures (α-lactose monohydrate and 4-aminobenzoic acid) in liquids. The results demonstrate that the absorption spectra of the aqueous biomolecular mixtures exhibit an accumulation of THz features of each pure product, i.e., the amplitude of the absorption peaks is proportional to the mixing ratio, while the corresponding absorption baseline increases with decreasing concentration. Furthermore, the content of the target substance can be calculated from the linear relationship between the absorption spectra of pure and mixed samples. This technology will support the future application of THz-TDS in early disease diagnosis under complex states and environments.
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DRUG DELIVERY
·
2020
·
IF 4.902
Yuqi Yang,Yiyue Guo,You Xu,Yingying Meng,Xing Zhang,Xuejun Xia,YuLing Liu
Deformable nanovesicles (DNVs) have been used in the buccal delivery of biomacromolecules due to their ability to enhance drug penetration. However, no breakthroughs have been made until now due to limited understanding of the factors affecting in vivo buccal delivery. In this study, we designed a series of DNVs, based on an insulin–phospholipid complex (IPC-DNVs), to investigate the influence of drug dose, buccal administration methods, and key quality characteristics of IPC-DNVs for buccal delivery. IPC-DNVs showed a non-linear dose–response relationship between 8 and 12 IU. There was no significant effect of drug delivery site (sublingual mucosa/buccal mucosa) or ligation time (15 or 30 min) on buccal absorption of IPC-DNVs. However, the area above the curve of reduction in blood glucose level overtime (AAC0–6h) for oral mucosa administration was significantly higher than that for buccal mucosa administration. Increasing the drug concentration in IPC-DNVs led to a decrease in AAC0–6h. This might be due to local leakage of DNVs, while squeezing through biological barriers with high concentration of insulin, thus hindering the subsequent delivery of DNVs. IPC-DNVs, measuring 80–220 nm in size, did not significantly affect AAC0–6h. However, when the size was increased to approximately 400 nm, AAC0–6h decreased, thus suggesting that IPC-DNVs with reasonable size were more effective. Additionally, increased deformability of IPC-DNVs might cause drugs to leak easily, thus reducing the promoting effect of buccal absorption. Our results clarified the effect of characteristics of IPC-DNVs on buccal delivery in vivo and provided meaningful support for the design of dosage form of DNVs.
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Pharmaceutics
·
2019
·Vol. 11
·期 10
·539
·
IF 4.773
You Xu,Yiyue Guo,Yuqi Yang,Yingying Meng,Xuejun Xia,Yuling Liu
Deformable nanovesicles have been extensively investigated due to their excellent ability to penetrate biological barriers. However, suffering from serious physical and chemical instabilities, the wide use of deformable nanovesicles in medical applications is still limited. Moreover, far less work has been done to pursue the lyophilization of deformable nanovesicles. Here, we aimed to obtain stable deformable nanovesicles via freeze-drying technology and to uncover the underlying protection mechanisms. Firstly, the density of nanovesicles before freeze-drying, the effect of different kinds of cryoprotectants, and the types of different reconstituted solvents after lyophilization were investigated in detail to obtain stable deformable nanovesicles based on insulin-phospholipid complex (IPC-DNVs). To further investigate the underlying protection mechanisms, we performed a variety of analyses. We found that deformable nanovesicles at a low density containing 8% lactose and trehalose in a ratio of 1:4 (8%-L-T) have a spherical shape, smooth surface morphology in the lyophilized state, a whorl-like structure, high entrapment efficiency, and deformability after reconstitution. Importantly, the integrity of IPC, as well as the secondary structure of insulin, were well protected. Accelerated stability studies demonstrated that 8%-L-T remained highly stable during storage for 6 months at 25 °C. Based on in vivo results, lyophilized IPC-DNVs retained their bioactivity and had good efficacy. Given the convenience of preparation and long term stability, the use of combined cryoprotectants in a proper ratio to protect stable nanovesicles indicates strong potential for industrial production.Keywords:deformable nanovesicles;combination;cryoprotectant;mechanism;insulin
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JOURNAL OF SEPARATION SCIENCE
·
2023
·Vol. 46
·期 20
·2300384
·
IF 3.1
Zheng Jiang,Hong Wang,Miao Yu,Cheng Qu,Wei Yue,Qinan Wu
In this study, a low-cost efficient online derivatization system was developed which allows for the detection of various types of mono- and oligo-saccharides only utilizing high-performance liquid chromatography (HPLC)-ultraviolet detector (UV) system. In the proposed method, phenylhydrazine was used as the derivatization reagent and directly spiked in the mobile phase, allowing for the separation and detection of mono- and oligosaccharides in an accessible instrument system (HPLC-UV). And the online derivatization design of the proposed method has significantly reduced the potential harm of derivatization reagents to the analysts. Furthermore, critical chromatographic parameters were optimized via the Box-Behnken design strategy, culminating in the ideal response for saccharides. Finally, the methodology validation of the proposed method was conducted. The proposed method showed satisfactory linear ranges with acceptable correlation coefficients (R 2 > 0.99), outstanding accuracy (Recovery: 95.3%–105.6%), high intra-day precision (relative standard deviation [RSD]: 1.4%–7.1%) and inter-day precision (RSD: 2.0%–7.4%). The robustness and ruggedness of the proposed method were proved as the recovery values in the range of 95.0%–104.6% and 95.1%–104.8% for robustness and ruggedness, respectively. These satisfactory validation results confirm the applicability and reliability of the proposed method for the analysis of saccharides in various complex real-world samples.
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ACS Sustainable Chemistry & Engineering
·
2019
·
IF 6.97
Rui Li,Jinhu Wang,Yi He,Faqin Dong,Liang Bian,Bowen Li
Defect engineering on metal oxide semiconductors is considered as a versatile approach to regulate their properties. The traditional method for preparation of defective metal oxide semiconductors always requires harsh reaction conditions such as high temperature, high pressure, and explosive chemicals. Here we report an environmentally friendly mechanochemical synthesis way for preparation of several defective metal oxide semiconductors (MoO3, TiO2, and ZnO) in 10 g quantities by grinding semiconductor powders with ascorbic acid. The resulting vacancy-rich TiO2 and ZnO show a strong visible-light absorption. Meanwhile, the obtained hydrogen-doped MoO3 (H0.5MoO3) exhibits an exceptional localized surface plasmon resonance (LSPR), which follows Prout-Tompkins B1 solid-state reaction model. In addition, the plasmonic H0.5MoO3 is further utilized for colorimetric detection of H2O2. This colorimetric assay is able to determine concentrations of H2O2 from 0.05 to 3 mM with a detection limit of 30 μM. It can distinguish the presence of 3 mM H2O2 by the naked eye.
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Analytical Methods
·
2024
·
IF 2.7
Changmao Yang,Yue Xiao,Yunjun Yan,Houjin Zhang
The development of straightforward and cost-efficient methods for bacterial identification is very important. In this study, we utilized xylenol orange, metal ions, and diverse bacterial carbon sources to construct a sensor array, achieving precise bacterial identification. Initially, we examined the absorbance variations of xylenol orange with five metal ions (Mn2+, Co2+, Ni2+, Cu2+, and Zn2+) at pH levels ranging from 4 to 7, observing significant changes at 570 nm or 580 nm. Given that bacteria can generate varying amounts of acid in different carbon sources, we employed a blend of xylenol orange and the five metal ions as pH-sensitive probes to characterize bacterial metabolism in three carbon sources, resulting in the development of a five-sensor array that effectively differentiated seven bacteria. Additionally, by utilizing xylenol orange with Co2+, we successfully identified six different bacterial mixtures and five types of probiotic drink products. These findings highlight the potential of our methods for broader practical application in bacterial identification in practical use.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Wei Cheng,Huilin Wen,Haixia Shen,Chenwei Fu,Erkuang Miao,Dongbao Li,Xiaoqiang Chen,Chi Hu,Ziyi Yu
Polymer hydrogels are instrumental in cell immobilization for biomanufacturing, yet traditional hydrogel bioreactors often compromise cell viability, function, and mass transfer. This results in uncontrolled microbial proliferation and significant cell leakage, thereby impeding the continuous production of high-value products. To address these challenges, we introduce an innovative liquid microcavity technique employing a blend of Gelatin-methacryloyl (Gel-MA) and Pluronic F127diacrylate (F127-DA) for precision bioprinting, followed by selective enzymatic digestion of gelatin to create microbial habitats. Through tailoring of the hydrogel’s pore structure and integration of diverse networks, our approach facilitates controlled nutrient transfer, efficient waste removal, and stable mechanical support, all while maintaining a leak-free suspension culture of microorganisms. We validated the effectiveness of our method by fabricating yeast-infused Three-dimensional (3D) lattice constructs, successfully achieving continuous fermentation of glucose into ethanol in a flow reactor with minimal efficiency reduction (≥90 % yield) over five cycles. This research not only confronts the spatial limitations inherent in hydrogel-based systems but also demonstrates a foundational potential for revolutionary change in biomanufacturing practices.
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APPLIED CLAY SCIENCE
·
2024
·
IF 5.3
Ping Chen,Hui Zhong,Xiao-rong Li,Tingting Zhang,Xiang Wang,Fang Hu,Yubo Yan,Jiaxing Xu,Meisheng Li
A novel nanozyme, based on copper-ion-complexed palygorskite (Pal Cu 2+ ), was synthesized using a facile method. The introduction of Cu 2+ demonstrates a synergistic effect with Pal, enhancing the peroxidase-like activity for the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H 2 O 2 . Steady-state kinetic analysis revealed that Pal Cu 2+ exhibits a higher affinity for substrates compared to native horseradish peroxidase, presenting a practical alternative to natural peroxidase. The efficacy of Pal Cu 2+ as a peroxidase mimic for H 2 O 2 sensing was investigated through colorimetric methods. Additionally, when combined with enzymatic glucose oxidation, a sensitive and selective colorimetric strategy for glucose detection was established. The developed method was effectively applied to detect H 2 O 2 and glucose in real samples. The proposed strategy offers an approach for the rational design and development of metal ion composites, opening new way for future applications of Pal-based materials.
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