LANGMUIR
·
2025
·
IF 3.9
Jiaqi Xia,Shuangshuang Chen,Zhencai Guo,Cheng Chen,Jing Zhang,Zhiwei Zhang,Lixia Jia
By utilizing the etching reaction between a hydrophobic copper mesh (CuM) and an alkali solution to obtain a superhydrophilic copper mesh (AT CuM), and then only depending on the modification of linoleic acid (LA) at room temperature, a fluorine-free superhydrophobic copper-based material (CuM@Cu(OH)2@LA) was fabricated. Subsequently, a funnel-shaped fog collector was constructed by simple folding for fog collection, which collected 10 400 mg of water with a water-harvesting rate (WHR) of 13.9 mg min–1 cm–2 after 30 min. Meanwhile, the AT CuM was deposited with polydopamine to obtain a photothermal nonfluorinated superhydrophobic copper base material (CuM@Cu(OH)2/PDA@LA). CuM@Cu(OH)2/PDA@LA was folded in the shape of a boat, filled with degreasing cotton, and placed upside down for seawater desalination as the (CuM@Cu(OH)2/PDA@LA)/degreasing cotton photothermal interface evaporation device ([(CuM@Cu(OH)2/PDA@LA)/DC]-PTIED). Thanks to its photothermal effect and superhydrophobic property providing the possibility of desalination, the evaporation device was capable of photothermal heating and floating on the water, achieving an evaporation rate of up to 1.85 kg m–2 h–1 under seawater outdoor simulation test conditions. Condensed water, which was desalinated by [(CuM@Cu(OH)2/PDA@LA)/DC]-PTIED, met the World Health Organization (WHO) drinking water standards. In terms of durability analysis, both products maintained good wettability and performance after continuous 12 h mechanical abrasion, immersion in water, and UV irradiation. As a result, these two copper-based non-fluorine superhydrophobic materials had the advantages of superior performance, a simple preparation path, convenient operation, a mild reaction process, sustainable energy saving, and good application durability, providing a new perspective for the large-scale and low-cost production of freshwater resources in a low-carbon and sustainable way in underdeveloped areas.
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Food Bioscience
·
2026
·
IF 6.2
Jing Cui,Lulu Chang,Bo Yang,Jianxin Zhao,Wei Chen,Haiqin Chen
The membrane-bound linoleic acid isomerase from B. breve CCFM683 (BBI) is a key biocatalyst for the conversion of linoleic acid (LA) to conjugated linoleic acid ( c 9, t 11-CLA). However, its intrinsic membrane-associated nature severely limits the efficiency of heterologous expression. To address this, we employed a fusion tag strategy to enhance solubility and improve protein expression levels. We introduced the solubilizing tag NusA and a FLAG tag for detection, systematically evaluating their effects on BBI expression and function. Protein solubility and CLA bioconversion activity were assessed through Western blot analysis of recombinant proteins and GC-MS quantification of products. The results demonstrated that NusA increased BBI expression, raising the soluble fraction from 9.52% to 66.49%, but significantly reduced enzymatic activity. To reconcile the trade-off between solubility and activity, we incorporated a flexible linker peptide between BBI and NusA. This modification enhanced total BBI expression by 3.49-fold, increased the soluble ratio to 95.06%, and restored CLA bioconversion activity to 97.23%, representing a 3.82-fold improvement compared to the construct without the linker. Molecular docking analysis suggested that the underlying mechanism may involve steric hindrance imposed by NusA on BBI, which was alleviated by the physical separation afforded by the linker. This study highlights the regulatory role of domain conformational flexibility in transmembrane protein function and offers an effective strategy for the soluble expression and rational design of complex membrane proteins.
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CARBOHYDRATE POLYMERS
·
2026
·
IF 13.2
Bo Zheng,Hui Wen,Xingwei Xiang,Bin Zheng
This study elucidates how dynamic high-pressure microfluidization (DHPM) synergistically combined with ultrasound (US) pretreatment regulates the molecular assembly, hierarchical structure, and in vitro digestive behaviors of starch-unsaturated fatty acid (UFA) complexes. Fully gelatinized wheat starch (WS) was complexed with trans-oleic acid (EA)/linoleic acid (LA) and processed by US-assisted DHPM. Distinct digestion behaviors were observed, with WS-LA complexes exhibiting a significantly higher resistant starch (RS) content up to 21% than the EA counterparts. Structural analyses revealed that US pretreatment enhanced amylose chain mobility and promoted the formation of single-helical inclusion structures, while subsequent DHPM intensified starch-lipid interactions and drove the self-assembly of compact supramolecular domains. Owing to its near-linear conformation, EA favored deeper helix insertion and higher V -type crystallinity, whereas LA was associated with fewer but potentially more densely organized nanostructures. Correlation analysis demonstrated that digestive resistance and release rate was governed not only by the extent of inclusion complexation, but more critically by the hierarchical organization of assemblies. These findings provide mechanistic insights into structure-digestibility relationships and offer guidance for designing starch-based systems with tailored digestion properties.
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International Journal of Coal Science & Technology
·
2026
·Vol. 13
·期 1
·15
·
IF 10.1
Hao Haijing,Zhu Ying,Zhong Mei,Liu Yang,Dai Zhenghua,Jin Lijun
Oil-soluble catalyst is a promising alternative to non-oil-soluble catalyst due to excellent dispensability in coal-oil slurry and relatively high activity. To reveal evolution behavior of active phase of the oil-soluble Fe-based catalysts in direct liquefaction of Shangwan coal, three oil-soluble Fe-based catalysts (FeLA, FeOA, FeSA) were synthesized via saponification method with linoleic acid, oleic acid and stearic acid as organic ligands, and were compared with two traditional iron oxide catalysts (Fe 2 O 3 , FeOOH). The evolution of active compositions during different temperatures was studied by XRD, SEM, XPS and HRTEM. Results demonstrated superior stability and catalytic activity of oil-soluble catalyst during direct liquefaction. TG and FTIR analyses confirmed the decomposition at lower temperatures, and formed active phases more readily than Fe 2 O 3 and FeOOH catalysts. The oil yields were significantly improved up to 62.82 wt%, 64.38 wt% and 66.36 wt% for FeLA, FeOA and FeSA, which were higher than those of Fe 2 O 3 and FeOOH catalysts. FeOA and FeLA catalysts particularly excelled in promoting the formation of cycloalkanes, thereby improving oil quality. The characterizations by XRD and XPS analyses of the samples indicated that these oil-soluble catalysts rapidly form Fe 0.875 S at lower temperatures, avoiding intermediate oxide phases. Smaller crystallite sizes were produced for oil-soluble catalysts compared than Fe 2 O 3 and FeOOH. Especially, substantial amounts of Fe 0.875 S were generated for FeSA during initial coal pyrolysis, which activated hydrogen to stabilize coal macromolecular radicals and enhanced coal conversion and yield. This work provides a guide for the development of highly efficient catalysts in direct coal liquefaction.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2026
·
IF 3.8
Qin Ke,Cui Jiaqi,Zhang Yajing,Zhao Wuduo,Zhang Qidong,Xi Hui,Fu Yingjie,Wang Dingzhong
Fatty acids (FAs) are essential components of lipid metabolism and play crucial roles in biological systems. However, due to their low abundance and poor ionization efficiency, the detection of FAs in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) poses significant challenges, making the spatial distribution of these molecules in tissues difficult to analyze. We present a novel on-tissue derivatization strategy for FAs using N,N-diethylethylenediamine (DEEA) as the derivatization reagent, which converts the carboxyl group of FAs into a positively charged moiety via amidation, thereby improving their ionization efficiency in positive ion mode. By systematically optimizing experimental parameters including catalyst type, reaction time, and matrix concentration, combined with α -cyano-4-hydroxycinnamic acid (CHCA) matrix, in situ imaging of nine key FAs (palmitic acid, linoleic acid, eicosapentaenoic acid and docosahexaenoic acid, etc.) was successfully achieved in rat kidney tissues. The derivatization products were verified by ESI-Q-TOF-MS, confirming the reliability of the method. Furthermore, comparative analysis with the conventional derivatization reagent 2-picolylamine (PA) demonstrated that DEEA markedly enhanced the derivatization efficiency of FAs. This study employs DEEA as a derivatization reagent for MALDI imaging of FAs. This derivatization method effectively enhances the ionization efficiency of FAs in the positive ion mode of MALDI-TOF-MS, thereby providing a new and referable approach for the imaging of FAs in biological tissues. Graphical
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ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2026
·Vol. 60
·期 32
·22813-22822
·
IF 12.2
Gao Jigang,Liu Peiqi,Huang Yukai,Hu Yulong,Zhou Zhongyue,Yuan Wenhao,Zeng Meirong
Lipid oxidation driven by intermolecular interactions
plays a pivotal
role in human aging and indoor environmental quality, yet the underlying
interaction mechanisms remain largely elusive. Here, we uncover a
coupled autoxidation network in the heterogeneous co-ozonolysis of
model skin lipids (cholesterol and fatty acids), using online atmospheric
pressure photoionization coupled with an ultrahigh-resolution mass
spectrometer. Individual cholesterol exhibited extremely low heterogeneous
reactivity (determined as effective uptake coefficient, γ eff, Chol = 2.94 × 10 –6 ). However,
upon co-ozonolysis with fatty acids (oleic or linoleic acid), its
reactivity was enhanced by 31-fold (γ eff, Chol = 9.11 × 10 –5 when mixed with linoleic acid),
and elevated relative humidity further amplified this effect by an
additional 3-fold. The identification of α-alkoxyalkyl hydroperoxides,
α-acyloxyalkyl hydroperoxides, hydroxyalkyl hydroperoxides,
and secondary ozonides provides direct molecular evidence that Criegee
intermediates (CIs) drive the co-ozonolysis interaction network. Furthermore,
the detection of highly oxygenated molecules reveals that • OH reactions are triggered by CIs and hydroperoxide decomposition,
establishing a free-radical chain propagation pathway that further
accelerates autoxidation. We propose a novel interactive lipid autoxidation
mechanism: initiated by lipids’ co-ozonolysis, propagated via
CI-driven interactions, and amplified by • OH-mediated
radical chemistry. These findings unveil a coupling mechanism of CIs
and • OH pathways in governing the heterogeneous
reactivity of naturally coexisting lipids, providing fundamental insights
into their impacts on biological aging and environmental evolution.
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APPLIED CATALYSIS B-ENVIRONMENTAL
·
2026
·
IF 19.7
Bin Chen,Xingwen Cha,Bo Jiang,Yicong Chen,Jian Tian,Dandan Song,Zhongliang Huang,Xiaodong Zhang,Jiale Huang,Qingbiao Li,Guowu Zhan
Synergistic glycerol-grease pyrolysis yields high aromatics and deoxygenation rate • Olefin-aldehyde coupling at acid sites reduces carbon loss and boosts aromatization • DFT proves coupling thermodynamically favored over conventional aromatization • Broad feedstock scope and LCA‑confirmed environmental benefits The inefficient valorization of crude glycerol, a major biodiesel byproduct, severely limits the carbon economy of biorefining. Herein, we report a catalytic co‑pyrolysis strategy that synergistically converts crude glycerol and waste grease into aromatics over a rice husk silica-derived Ni/ZSM-5 catalyst, improving carbon utilization. The synergy is driven by an olefin-aldehyde coupling pathway on the acid sites of ZSM-5, where glycerol‑derived propenal reacts with grease‑derived olefins to form readily aromatized cyclic intermediates, minimizing secondary cracking and redirecting carbon toward liquid aromatics. This achieves >85.0% aromatic selectivity with a space-time yield of 1.8 g arom ·h -1 ·g catal -1 , representing an eightfold enhancement over glycerol‑alone catalytic pyrolysis. This coupling mechanism is thermodynamically superior to the traditional Ni-mediated aromatization pathway. Its applicability to diverse fatty acids and real waste oils highlights broad feedstock adaptability. This work establishes a scalable carbon‑efficient route for aromatic production from wastes, offering lower carbon footprint and higher energy gain than conventional alternatives. Download: Download high-res image (233KB) Download: Download full-size image
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Bo Zheng,Hui Wen,Xingwei Xiang,Bin Zheng,Tianjie Li
UFA molecular geometry directs hierarchical assembly of starch-lipid systems. • US-DHPM induces UFA-templated starch reassembly from helices to aggregates. • C18 UFAs promote ordered lamellar barriers and enhanced digestion resistance. • Long-chain PUFAs generate strain-induced heterogeneity and rapid digestion. • Digestive resistance is governed by barrier continuity rather than CI alone. The influence of unsaturated fatty acid molecular geometry on the assembly and digestive properties of starch-lipid complexes remains unclear. Here, five UFAs with distinct chain lengths and configurations were incorporated into gelatinized wheat starch via ultrasound-assisted dynamic high-pressure microfluidization to construct hierarchical complexes. Multiscale analyses showed that UFA geometry governed amylose helix inclusion, V-type crystallinity, and lamellar packing. Monounsaturated UFAs promoted compact ordered assemblies, whereas highly unsaturated UFAs generated looser interfacial structures. These differences were reflected in digestion behavior, with oleic-acid-based complexes exhibiting enhanced digestion resistance and delayed lipid release, while certain highly unsaturated systems such as DHA showed accelerated starch hydrolysis and fatty acid liberation. Notably the linoleic acid-based complex exhibited the highest resistant starch content of 20.77% and the slowest fatty acid release with 29.70% of fatty acids still retained after 120 min of digestion. Correlation analysis and molecular dynamics simulations further indicated that digestive resistance depends on the continuity of hierarchical packing rather than complexation efficiency alone. These findings clarify geometry-dependent structure-digestion relationships in starch-lipid complexes and support structure-guided design of systems with tunable digestive properties. Download: Download high-res image (203KB) Download: Download full-size image
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PHYTOMEDICINE
·
2026
·
IF 11.3
Xiao-Hui Ma,Jiang-Han-Zi Liu,Chang-Lun Chen,Cai-Sheng Wu,Di Lu,Liang-Liang He,Yan-Ping Wu,Yang Chen,Rong-Rong He,Tianhui Yuan
Background Wendan Tablet (WDT) is a traditional Chinese herbal compound formulation clinically employed in the management of coronary heart disease (CHD). Purpose This study aimed to elucidate the therapeutic mechanism of WDT in treating CHD and to identify its corresponding active constituents. Methods The primary components of WDT were identified, and a quantitative analytical method was established for quality control using UHPLC-QTOF-MS. We comprehensively evaluated the cardioprotective effects of WDT against ischemic injury and investigated its role in modulating phospholipid peroxidation of myocardial cells. Potential key molecular targets of WDT were predicted via molecular docking, and these predictions were subsequently validated through biochemical assays and transgenic mouse models. Results Mechanistically, WDT significantly improved cardiac function, reduced myocardial pathological damage, and suppressed lipid peroxidation. Specifically, it markedly downregulated the expression of arachidonate 15-lipoxygenase (ALOX15), a key enzyme involved in lipid peroxidation. Isocurcumenol was identified as the principal active component in WDT, demonstrating strong binding affinity for ALOX15. It effectively inhibited ALOX15 enzymatic activity and subsequently attenuated lipid peroxidation. While isocurcumenol conferred significant cardioprotection in the context of ALOX15 overexpression, both isocurcumenol and WDT lost their protective effects in ALOX15-deficient models. Conclusion This study demonstrates that WDT alleviates myocardial ischemia by inhibiting ALOX15 to reduce lipid peroxidation. We identified isocurcumenol as its key active component, which directly binds to and inhibits ALOX15. These findings establish ALOX15 not only as a crucial mechanistic target but also as a potential biomarker for the cardioprotective application of WDT and isocurcumenol in CHD management.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Baoying Yang,Yuelin Li,Shanshan Zhang,Siqian Li,Mindan Wu,Mingfu Wang
Wheat starch–linoleic acid (WS–LA) complex is a type 5 resistant starch (RS5) with potential for developing low glycemic index (GI) foods enriched in ω-6 fatty acids. In this study, WS–LA complexes were prepared via high-pressure homogenization (HPH, 100 MPa, 0, 3, 5, 10 cycles) and subsequently ultrasound-treated (UT, 600 W, 10 min), yielding the UT (0 cycles), HPH-UT3, HPH-UT5, and HPH-UT10 samples. The formation of V -type inclusion complexes was confirmed by XRD and solid-state NMR. Among all complexes, the HPH-UT5 sample exhibited the best slow digestion performance, with RS content increasing from 10.54% (native starch) to 18.05%, and the slowly digestible starch content reaching the maximum of 16.60% ( p < 0.05). In addition to its nutritional benefits, the HPH-UT10 sample demonstrated potential as a starch-based Pickering stabilizer, with emulsions showing no phase separation over 28 days at room temperature. Overall, the HPH-UT sequence offers an effective strategy for preparing RS5 with enhanced resistant starch content and emulsion-stabilizing capability, providing a promising route toward low-GI foods enriched with ω-6 fatty acids.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2026
·Vol. 27
·期 3
·1216
·
IF 4.9
Viktor A. Timoshnikov,Vladimir E. Koshman,Aleksandr A. Deriskiba,Nikolay E. Polyakov,George J. Kontoghiorghes
Salicyluric acid (SUA), the main metabolite of aspirin and a natural product, is known for its ability to chelate iron and other metal ions. In particular, the chelation and increased excretion of iron by SUA may contribute to the aspirin-induced iron deficiency anemia observed in long-term aspirin users. The redox activity of iron and copper complexes of drugs and also drug metabolites, such as SUA, is an important parameter of their overall toxicity profile, including the induction of ferroptosis, which has been associated with many diseases. In this context, the effect of SUA on iron- and copper-induced lipid peroxidation and also its localization within a model lipid membrane have been investigated. A combination of physicochemical methods, including Nuclear Magnetic Resonance (1H NMR), molecular dynamics (MD), and Nuclear Overhauser Effect Spectroscopy (1H NOESY), has been used to demonstrate that SUA does not promote the peroxidation of linoleic acid micelles in the presence of Fe(II) or Cu(II) ions. NMR experiments revealed that SUA incorporates into the lipid bilayer, which stabilizes the ligands and inhibits its metal chelation ability in comparison to the control. NOESY experiments and MD simulations further showed that SUA localizes shallowly within the membrane, interacting primarily with the head group and upper acyl chain regions of lipids. These findings provide crucial insights into the membrane redox reactivity and other behavior of SUA, explaining its lack of pro-oxidant activity and also highlighting its complex role in the pharmacological and toxicological effects on iron metabolism in long-term aspirin users.
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Science China-Life Sciences
·
2026
·
IF 9.6
Wang Bao,Fu Junpeng,Qian Jian,Wei Yuang,Bao Meiling,Miao Haoqi,Zhao Xinzhe,Shao Kezheng,Song Yuan,Tan Ruoyun,Li Jie,Shao Pengfei
Clear cell renal cell carcinoma (ccRCC) exhibits aberrant lipid synthesis, yet how this metabolic reprogramming influences ferroptosis—a form of regulated cell death driven by lipid peroxidation—remains poorly understood. This study demonstrates that the key lipid transcription factor SREBP1 acts as a master regulator that promotes ccRCC progression by suppressing ferroptosis. Using SREBP1-knockdown cell models, we found that SREBP1 depletion enriches ferroptosis-related pathways and enhances ferroptosis sensitivity, as evidenced by elevated mitochondrial ROS, labile iron pool, PE-OOH levels, and characteristic mitochondrial alterations. Mechanistically, SREBP1 transcriptionally represses the pro-ferroptotic gene ACSL4 while simultaneously activating the lipid-synthesis gene FASN . Rescue experiments revealed that modulating either ACSL4 or FASN alone only partially reverses the ferroptosis phenotype induced by SREBP1 loss, whereas dual manipulation of both genes fully restores ferroptosis resistance. Metabolomic analyses showed that ACSL4 and FASN converge to regulate the cellular PUFAs/MUFAs ratio, which in turn feeds back to modulate SREBP1 nuclear translocation, thereby establishing a self-reinforcing positive-feedback loop. Clinically, low ACSL4 expression correlates with advanced tumor stage and poor patient survival. In vivo, SREBP1 promotes tumor growth by inhibiting ferroptosis via the ACSL4 / FASN axis. Collectively, our findings reveal that SREBP1 drives ccRCC malignancy through a dual-pronged mechanism—repressing ACSL4 and activating FASN—to alter the PUFAs/MUFAs balance and establish a feed-forward loop that sustains ferroptosis suppression. ACSL4 represents a potential prognostic biomarker, and targeting the SREBP1-ACSL4 / FASN axis offers a promising therapeutic strategy for ccRCC.
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