JOURNAL OF CONTROLLED RELEASE
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2025
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IF 11.5
Peng Jiang,Yongnian Zeng,Wen Zhang,Jiapeng Lei,Xue Jiang,Wei Li
Reactive oxygen species (ROS)-based therapies have emerged as promising non-antibiotic strategies for treating infected wounds due to their broad-spectrum antimicrobial activity and low risk of resistance development. However, their clinical translation remains limited by low delivery efficiency, poor bacterial targeting, and uncontrolled post-treatment inflammation. To address these challenges, we reported a multifunctional bilayer microneedle patch (LFT/S-MN) designed for efficient bacterial eradication and enhanced wound healing. The patch integrates positively charged lactoferrin-based nanoparticles (LFT NPs) conjugated with a porphyrin sonosensitizer (TAPPFe) in the dissolvable base layer, and a pH-responsive tip layer loaded with the anti-inflammatory agent spermidine (SPD). Upon insertion into infected tissue, the released LFT NPs efficiently target and aggregate negatively charged bacteria, enabling robust ROS generation through synergistic chemodynamic and sonodynamic therapy (CDT/SDT) under ultrasound stimulation. Concurrently, the acidic wound microenvironment triggers the gradual degradation of the tip layer, allowing controlled SPD release for immunomodulation. In a murine P. aeruginosa -infected wound model, the LFT/S-MN patch significantly reduced bacterial load, suppressed inflammation, promoted collagen deposition and neovascularization, and accelerated wound closure. This work presents a promising non-antibiotic strategy for wound disinfection and regeneration through synergistic CDT/SDT and inflammation modulation.
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Bioactive Materials
·
2026
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IF 20.3
Weiwei Yu,Xiang Li,Xufang Liu,Xinyi Hao,Wen Qin,Guoqing Qi,Gaopeng Dang,Ziyuan Tian,Shiyin Jin,Conrado Aparicio,Kaiyan Wang,Wen Niu,Lina Niu
Oral ulceration is a prevalent mucosal disorder, and its healing process is frequently hampered by poor local drug retention and inadequate mucosal adhesion. However, the underlying pathological mechanisms remain incompletely elucidated. In this study, transcriptomic sequencing revealed that ferroptosis serves as a key driver of oral ulcer progression. To address the limitations of conventional formulations in the wet and dynamic oral environment, we constructed an asymmetrically adhesive, ferroptosis-targeting, spermidine (SPD)-functionalized Janus hydrogel microneedle system (MN-HTSO-C). This system was fabricated via chemical grafting and dynamic Schiff base crosslinking, enabling targeted drug delivery into mucosal tissues. By specifically delivering SPD, this system effectively inhibited ferroptosis, reduced reactive oxygen species (ROS) accumulation, reprogrammed the local immune microenvironment, and thereby promoted angiogenesis and epithelial regeneration. Our work not only identifies a novel pathological mechanism but also proposes an integrated therapeutic strategy that combines targeted delivery, immunomodulation, and ferroptosis inhibition, providing a new direction for the treatment of oral ulcers and other ferroptosis-related mucosal diseases.
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PLoS One
·
2026
·Vol. 21
·期 1
·e0339776
·
IF 2.6
Keming Ying,Han Xue,Shenheng Du,Xin Wang,Xiaoyun Lei
N1,N12-diacetylspermine (DiAcSpm), a promising biomarker for cancer diagnosis, presents significant quantification challenges due to the structural homology within the polyamine family. To address this issue, we engineered a molecularly imprinted monolithic (MIM) column functionalized with biomimetic phosphorylcholine (PC) as functional monomer for the selective recognition of DiAcSpm in human urine. The zwitterionic polymer was synthesized via thermally initiated polymerization, with its morphology and pore architecture characterized through scanning electron microscopy (SEM) and brunauer-emmett-teller (BET) analysis. After optimizing capillary microextraction (CME) parameters, the MIM demonstrated a broad linear response (10–500 μM), a low detection limit (3.3 μM, S/N = 3), and high recoveries (76.8–91.2%) when coupled with HPLC-UV analysis. The biomimetic PC-based recognition significantly improved selectivity against key structural analogs, such as spermine, in complex biological matrices. This study underscores the potential of zwitterionic-based MIMs as a robust and efficient platform for the sensitive and selective monitoring of acetylated polyamines in clinical settings.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2026
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IF 4.1
Tang Xiaolu,Li Quan,Yang Yue,Wu Meilin,Liu Qingqing,Mo Zhihong
Protein-rich foods release biogenic amines (BAs) during prolonged storage, and these compounds serve as key biomarkers of food spoilage. Current separation-based methods for detecting BAs in food require sophisticated instrumentation and laborious sample pretreatment procedures. The array-sensing strategy, which mimics biological olfaction, provides a simple and rapid approach for the simultaneous detection of multiple BAs without the need for separation. Here, we constructed a multidimensional fluorescent sensor array using porphyrin-functionalized carbon dots (P-CDs) and their composites with metal ions (M-P-CDs), thereby incorporating the excitation wavelength as an additional signal dimension. This design reduces the number of physical sensors required while maintaining high discrimination capability, thereby overcoming a key limitation of conventional sensor arrays that depend solely on increasing the number of sensors. With the support of chemometric and machine learning methods, the sensor array successfully discriminated among ten BAs. We achieved quantitative detection of putrescine, histamine, and tyramine, determining their limits of detection (LODs) to be 2.37, 2.96, and 1.55 mg/L, respectively. The sensor array exhibited excellent stability (i.e., no significant fluorescence change over 30 days) and high selectivity against common interfering substances. Validation experiments on milk samples demonstrated the array’s ability to reliably discriminate specimens at different spoilage stages. This work establishes a novel multidimensional array-sensing strategy for detecting BAs, an approach that holds great potential for food safety applications. Graphical
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Yanan Liu,Yuhao Huang,Qileng Aori,Yu Wang,Sai Zhao,Zhen-Lin Xu
Antibacterial hydrogel packaging often adheres poorly to hydrophobic prefabricated meat surfaces. Metal-polyphenol networks, combining metal ion antibacterial properties with polyphenol adhesion, offer promising preservation potential. In this study, tannic acid -Fe III metal polyphenol network-coated gallic acid -loaded ovalbumin/chitosan nanoparticles with adhesion and sustainable antibacterial properties were prepared and embedded into the gelatin hydrogel to elongate the shelf life of prefabricated meat. The composite hydrogel exhibited high adhesion, with an average adhesion force of 1.4-fold ∼1.9-fold larger than the other groups. It also exhibited good antibacterial properties, the colony-forming units (CFU) of 4 type of bacteria was reduced by 0.69, 0.34, 1.05 and 0.90 log 10 (CFU/mL). The composite hydrogel delayed pH, total volatile basic nitrogen (TVB-N), microorganism growth, and the marginally acceptable limit was delayed by 4 days compared with the other groups in applications. These results demonstrate the composite hydrogel's significant potential as a preservation material for prefabricated meat.
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BIOMATERIALS
·
2026
·
IF 13.6
Zheng Deng,Xirui Wu,Tianhao Huang,Huiyuan Chen,Zhuochao Wu,Li Liu,Yuqian Liu,Chunjie Wang,Zijian Xiong,Zhuang Liu,Guangbao Yang
Intracellular polyamine metabolic homeostasis is closely linked to therapeutic outcomes in cancer. However, their intrinsic antitumor mechanisms have not been fully clarified. Herein, we demonstrate that the catabolism of spermine (Spm) and spermidine (Spd) mediated by polyamine oxidase directly generates two potent cytotoxic metabolites, namely acrolein and hydrogen peroxide. These metabolites act synergistically to trigger a marked elevation in intracellular reactive oxygen species (ROS), which in turn activate the caspase-1/GSDMD and caspase-3/GSDME signaling pathways, ultimately driving tumor cells into pyroptosis. Based on these findings, we develop a phenylboronic acid-functionalized sodium alginate-based hydrogel controlled-release system (Alg-PBA/Spd). This platform achieves sustained Spd release through coordination interactions and markedly inhibits tumor growth by inducing pyroptosis. Further studies demonstrate that the Alg-PBA/Spd hydrogel synergizes with radiotherapy (RT) to enhance radiosensitivity in an orthotopic breast cancer model and inhibit pulmonary metastasis. Moreover, this hydrogel enables co-delivery of αPD-L1 with sustained release, which significantly improves the response rate of immune checkpoint blockade therapy by inducing pyroptosis and remodeling the tumor immune microenvironment. Overall, this study not only elucidates a previously unrecognized mechanism underlying polyamine metabolism-driven pyroptosis but also offers a versatile platform to enhance the efficacy of RT and immunotherapy.
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FISH & SHELLFISH IMMUNOLOGY
·
2026
·
IF 4.2
Wenmei Li,Chenchen Guo,Kaixin Wu,Qunrui Xie,Jianye Yang,Aiwen Shen,Qin Liu,Yuhong Ren
Unmethylated cytosine-guanine oligodeoxyribonucleotides (CpG ODNs) are potent immunostimulants via toll-like receptor 9 (TLR9) recognition, but their application is limited by nuclease degradation and high costs. Herein, a designed ssDNA fragment (1826-F) was circularized with T4 DNA ligase and amplified by rolling-circle amplification (RCA) with Phi29 polymerase, self-assembling into RCA-1826 nanoparticles with a mean size of 63.05 nm and a zeta potential of −5.75 mV. Nuclease resistance assays were performed to evaluate degradation stability, showing RCA-1826 degraded slowly over 24 h while linear control 1826-R was fully degraded within 6 h. In vitro , murine macrophages (RAW264.7) and zebrafish liver cells (ZFL) were treated with RCA-1826, and the results demonstrated that inflammatory factor expression was significantly upregulated, verifying its immunostimulatory activity. In vivo , administration of 1 μg RCA-1826 induced robust innate immunity in zebrafish. Combined with inactivated vaccine, RCA-1826 acted as an effective adjuvant, conferring 94.4% protection against Edwardsiella piscicida . This protection rate is 11.1% higher than that of the sulfur-modified ODN1826-adjuvanted vaccine (83.3%). Overall, RCA-based synthesis enables stable, immunogenic CpG DNA nanoparticles, highlighting RCA-1826 as a promising candidate for E. piscicida prevention in aquaculture.
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CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Yumo Chen,Shenyuan Ouyang,Bingjie Tong,Mengjiao Zhang,Meng Liu,Yiying Jia,Ting Ouyang,Sunkuan Hu,Guangju Zhao,Helin Xu,Kaili Mao
Ulcerative colitis (UC) is a chronic inflammatory bowel disease characterized by oxidative stress, and impaired intestinal barrier. Current therapies often fail to address the multifaceted nature of the disease. Herein, we developed a novel therapeutic small-molecule coacervate microdroplets formed by the multiple physical interaction between thioctic acid (TA) and spermidine (SP). TA-SP coacervate microdroplet is easily prepared by simply dispersing TA powders in SP solution, without addition of any toxic organic solvents. Besides, this TA-SP coacervate microdroplet exhibits the exceptional wetting and mucoadhesive properties. Crucially, upon rectal administration, it undergoes an in-situ phase transition from a liquid-like microdroplet to a dense hydrogel, significantly prolonging its retention at the colonic site. In DSS-induced murine colitis model, TA-SP coacervate microdroplets demonstrated the superior therapeutic efficacy compared to individual components, effectively alleviating disease severity, reducing colon shortening, and restoring histological architecture. Moreover, the pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) inside colitis colon was potently suppressed by TA-SP coacervate microdroplets via promoting the restorative M2 macrophage phenotype. Importantly, it repaired the gut mucosal barrier by restoring goblet cell function, mucin secretion, and tight junction proteins (ZO-1, OCLN and CLDN-5). Besides, TA-SP coacervate could effectively normalized gut microbial ecosystem of colitis mice via suppressing the expansion of pro-inflammatory pathobionts while promoting the recovery of beneficial commensals. Mechanistic studies revealed dual modes of action, that was, TA-SP not only functioned as a ROS scavenger to rebalance the oxidative-redox homeostasis via the Nrf2-Keap1 pathway but also severed as a potent autophagy-inducer to repair the epithelial homeostasis. By integrating physical barrier function with synergistic pharmacological activities, this self-assembled coacervate represents a promising and robust platform for the local treatment of ulcerative colitis.
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Biomass Conversion and Biorefinery
·
2023
·
IF 4
Si Lin,Hou Juying,Lv Wei,Hou Qin,Xu Zhixiang,Ai Shiyun
In this paper, lignocellulose components were separated under mild conditions to achieve hierarchical utilization of biomass. Cellulose was retained in cellulose pulp with highly crystalline structure, and lignin powder was recovered, which offered a possibility for the high-value utilization of lignocellulosic biomass. The extracted lignin (EL) was carbonized and activated to prepare lignin-based porous carbon microspheres (LPCMs) and further used as a substrate to interact with fluorescein-5-isothiocyanate (5-FITC) for the detection of biogenic amines (BAs). The fluorescence of 5-FITC was found to be quenched in the presence of LPCMs. And then, upon addition of BAs, 5-FITC was released from the surface of LPCMs, which resulted in the fluorescence recovery of 5-FITC. Thus, a novel “turn off–on” fluorescent probe was constructed for sensitive and visual detection of BAs. Meanwhile, the retained cellulose skeleton structure was used to prepare a smart identification label for real-time and visual evaluation of the freshness of pork and shrimp. This work realized the improvement of comprehensive utilization rate of lignocellulose and developed a rapid, efficient, and sustainable method which combined natural lignocellulose materials with green analytical technology for visually evaluating food quality.
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JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Xu Xu,Jiaxin Gao,Di Cao,Xiang Li,Xin Zhao,Shuang Yue,Lei Zhang
In this paper, the novel 3D N-doped magnetic porous carbon spheres (3D N-MPCSs) was constructed via a simple template-free and self-doping method, followed by a direct carbonization of polymer with Fe 3+ for simultaneous activation and magnetization. For the first time, the designed 3D N-MPCSs was used as multifunctional (for extraction and magnetic separation) adsorbents for microwave-assisted derivatization and magnetic solid phase extraction, which exhibited excellent extraction ability for its large surface area, mesoporous structure, spherical morphology, and incorporation of N/O heteroatoms. Moreover, when the microwave-assisted derivatization was combined with the magnetic solid phase extraction, the extraction, derivatization and concentration was completed simultaneously within 135 s. Furthermore, this method has a low detection limit (0.059–0.073 ng mL −1 ) and high enhancement factors (333 < EFs < 382) for trace biogenic amine (BAs). The recoveries were between 92.3 and 108.8% with relative standard deviations less than 5.9%. Overall, a simple, rapid, efficient and sensitive method for the analysis of BAs in food samples was developed, which provided the basis for routine detection of food safety.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Yujuan Qin,Pengcheng Huang,Fang-Ying Wu
With food safety problems abounding, it is essential to explore some efficient and effective methods for monitoring food quality. To this end, we have developed a histamine-responsive carbon dots (CDs)-based sensing system for visual monitoring of food spoilage. In this system, the aldehyde group on the surface of CDs serves as the recognition sites to combine with the amino group on histamine to form a Schiff base that effectively enhances the fluorescence. On the other hand, 1-aminoanthraquinone (ANQ) was introduced as a reference signal to endow the fluorescent probe with self-calibrating capacity. As the histamine concentration increases, linear ratiometric response was obtained accompanied with a distinguishable fluorescence color change from red to blue. Besides, test strips-based smartphone visual assays were successfully used to evaluate and monitor the histamine levels in fish samples. Our proposed sensing strategy has been demonstrated to be efficient and robust along with great potential for real-time visual monitoring of food spoilage.
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CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Yuan-Fu Ding,Jianwen Wei,Xingping Quan,Wenting Gu,Long Xi,Ying Zheng,Yonghua Zhao,Jingwei Luo,Shengke Li,Greta S.P. Mok,Ruibing Wang
Psoriasis seriously harms physical and mental health of patients. Hyaluronic acid (HA)-based topical formulation can increase drug concentration in psoriatic skin via CD44-assisted targeting. Herein, we developed a supramolecular medicine composed of curcumin-loaded HA-cucurbit[7]uril (HA-CB[7]@Cur), which could efficiently sequester polyamines (PAs) via host-guest interactions of CB[7] and PAs to suppress RNA-PAs immunocomplex formation. Meanwhile, anti-psoriasis drug Cur could be released from HA-CB[7]@Cur by PAs. With phenotypical disease evaluation, psoriasis area measurements and severity index scoring, and histological characterizations, we demonstrate topical administration of Carbopol gel formulation of HA-CB[7]@Cur on psoriasis-like skin in mice exhibited an enhanced anti-psoriasis activity, in comparison with gel of free Cur or HA-CB[7]. Cytokine expression analysis in psoriatic skin also supported the observed therapeutic outcomes. We provide a novel and effective supramolecular strategy to realize cooperative anti-psoriasis via controlled release of curcumin and PAs sequestration, which can be potentially expanded to treat other PA-involved skin inflammatory diseases.
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