TALANTA
·
2020
·
IF 5.339
Can Chen,Changpeng Zou,Lin Li,Haidong Yu,Jixin Zhu,Jinhua Liu,Wei Huang
Levodopa, commonly used anti-Parkinson drugs in the clinic, is the most significant prodrug of dopamine that plays important roles in the treatment of Parkinson's disease. Therefore, monitoring content of levodopa of anti-parkinson drugs in human serum is extremely necessary. Herein, a simple, fast and low-cost method for levodopa detection is proposed depending on the in situ formation of blue and green emission fluorescent copolymer (FCP). The proposed method is based on the conversion of fluorescence emission peak of FCP from blue (430 nm) to green emission (535 nm) in 2 h. In this sensing system, both blue and green emission exhibit a high selectivity and sensitivity for levodopa determination in the range from 0 to 50 μM with a detection limit of 0.2 μM and 0.36 μM, respectively. Among them green emission FCP shows excellent recovery even at human serum concentrations up to 30%. Additionally, the proposed method was successfully applied to assess the content of levodopa in three anti-Parkinson drugs (carbidopa and levodopa CR tablets, levodopa and benserazide hydrochloride tablets, and levodopa tablets). More importantly, the levodopa determination of three anti-Parkinson drugs in human serum also exhibit an excellent recovery. Therefore, our strategy provides a promising method for mechanism study and treatment of Parkinson's disease.
研究领域:
生物化学
·
医学诊断
·
药物分析
✓ 已验证
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2017
·
IF 3.648
Hongmei Zhang,Yanqing Wang,Haidan Zhu,Zhenghao Fei,Jian Cao
Triclocarban (TCC) is an antibacterial agent in antibacterial personal care products. Human serum albumin (HSA) is the transport protein with the ligand binding properties. The current study was undertaken to identify the binding mechanism of TCC with HSA by using biophysical methods . The fluorescence and UV–vis spectral results showed that the fluorescence quenching of HSA by TCC was static quenching by the formation of HSA-TCC complex. The binding constants were obtained by molecular modeling and fluorescence quenching, and the results indicated the existence of strong interaction between HSA and TCC with binding constant K b ~ 10 5 L/mol. TCC can enter into the binding pocket of domain II of HSA by mainly hydrophobic and hydrogen bonds forces. The conformations of TCC and HSA are all changed during the binding interaction of them. We hope that this work will provide some useful information for understanding the activity and mechanism of antibacterial agent with the transport protein.
研究领域:
生物化学
·
药理学
✓ 已验证
DOI
高影响因子
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES
·
2015
·
IF 1.202
Huilun Chen,Honghao Rao,Jian Yang,Yongxiang Qiao,Fei Wang,Jun Yao
This investigation was undertaken to determine the interaction of diuron with human serum albumin (HSA) was studied by monitoring the spectral behavior of diuron-HSA system. The fluorescence of HSA at 340 nm excited at 230 nm was obviously quenched by diuron due to dynamic collision and the quenching constant was of the order of 104 L mol−1 at 310 K. However, no fluorescence quenching was observed when excited at 280 nm. Thermodynamic investigations revealed that the combination between diuron and HSA was entropy driven by predominantly hydrophobic interactions. The binding of diuron induced the drastic reduction in α-helix conformation and the significant enhancement in β-turn conformation of HSA. In addition, both sites marker competition study and molecular modeling simulation evidenced the binding of diuron to HSA primarily took place in subdomain IIIA (Sudlow's site II).
研究领域:
生物化学
·
药理学
·
分子生物学
✓ 已验证
DOI
高影响因子
DYES AND PIGMENTS
·
2020
·
IF 4.613
Junying Zheng,Jian Cao,Yajing Tu,Shuxin Wang
In the present strategy, a novel sensor Z2 of naphthalimide derivative was designed, synthesized and used to detect the sensitivity and selection of heparin. Z2 was suitable to detect heparin without obvious interference from counter ions and biomolecules in a wide pH range of 3–10. The mechanism of heparin detected by fluorescent sensor was due to the energy change of molecular from the structure rotation of the sensor. A limit of detection of Z2 sensing heparin was 7.56 ng/mL. Z2 can be used to detect heparin in 0.02% human serum sample.
研究领域:
分析化学
·
生物化学
·
医学科学
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2016
·
IF 2.74
Jiawei He,Di Wu,Yuanming Zhai,Qing Wang,Xiaoli Ma,Hongqin Yang,Hui Li
Understanding the binding mechanisms of drug–protein interactions is a crucial factor in determining the pharmacokinetics and pharmacodynamics of a drug. In this study, the binding affinity of inosine toward the HSA, as well as the dynamic properties of HSA-inosine complexes was obtained from applying NMR methodology. The changes in proton selective and non-selective relaxation rates suggested that inosine interacts with HSA. The values of normalized affinity indexes and equilibrium constant K provided information about the binding strength, which were in accordance with fluorescence result. The detailed binding mechanisms were obtained through fluorescence method , and the binding mode was displayed using Discovery Studio. Circular dichroism data further confirmed the secondary structure changes in HSA upon inosine addition. This study provided insights on the interactions between inosine and HSA.
研究领域:
生物化学
·
药理学
✓ 已验证
DOI
高影响因子
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
·
2023
·
IF 1.3
Zizhen Huang,Jie Wang,Hui Chen,Ruofei Feng,Chunxia Tan
Four organic-polyoxometalate hybrids BR4[SiW12O40] (BR–SiW), BR3[PMo12O40] (BR–PMo), BR4K[EuSiW11O40]·2H2O (BR–EuSiW) and BR6Na3[EuW10O36] (BR–EuW) were fabricated by the polyoxometalates (POMs) anions and berberine cations (BR) noted for the alkaloids in traditional Chinese herbal medicine. These hybrids have been characterized and confirmed. The interaction between hybrids and human serum albumin (HSA) was investigated in a buffer solution (pH 7.4) using ultraviolet–visible light absorption and fluorescence techniques. The classical Stern–Volmer equation was used to analyze the fluorescence quenching at three temperatures (296, 303 and 310 K), and the static quenching mechanism for interaction was proposed. The Thermodynamic parameters, enthalpy, entropy change, and Gibbs free energy of hybrids interacting on HSA were calculated by Scatchard equation. The results indicated that therewas one binding site on the protein and BR–POMs all showed stronger binding force than that of raw materials. Synchronous fluorescence results showed that the binding sites of BR–POMs and HSA were not effectively affected the surrounding microenvironment. The following antibacterial experiments implied that inhibitory effect of hybrids were synergistic effect from organic active ingredient and POMs but the simple combination. All these data were prepared for further research on biology.
研究领域:
生物化学
·
药理学
·
分子生物学
✓ 已验证
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Qinghua Hu,Changmin Yu,Xitao Xia,Fang Zeng,Shuizhu Wu
Biothiols like Cys, Hcy and GSH play important roles in various physiological and pathological processes, and it is still challenging to simultaneously and discriminatively detect GSH and Cys/Hcy due to their similar structures and reaction activities. Hence, it would be highly desirable to design a fluorescent probe for simultaneously discriminating GSH and Cys/Hcy in biological samples with no spectral crosstalk , few interferences and rapid response. Herein, through coupling two fluorophores with biothiol-sensitive linker, we developed a fluorescent probe , which has two biothiol-responsive and distinctly-separated emissions via independent visible-light excitations, for simultaneously discriminating GSH and Cys/Hcy with near-infrared and green emissions. The probe is operable in human serum samples, thus holding promise for diagnostic-related applications. Moreover, the probe shows quite good properties. First, it exhibits a rapid response (within a few minutes) with highly selective and sensitive detection for GSH and Cys/Hcy. Second, it offers an apparent colorimetric and two fluorescence emission signals without spectral crosstalk . Third, it shows low cytotoxicity. Therefore, it would provide a useful method for further elucidating the roles of biothiols as well as for conducting pathological analysis for diseases involving biothiols.
研究领域:
生物化学
·
分析化学
·
医学诊断
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2014
·
IF 3.259
Di Wu,Jin Yan,Jing Wang,Qing Wang,Hui Li
Binding interaction of human serum albumin (HSA) with allura red AC, a food colourant, was investigated at the molecular level through fluorescence, ultraviolet–visible, circular dichroism (CD) and Raman spectroscopies, as well as protein–ligand docking studies to better understand the chemical absorption, distribution and transportation of colourants. Results show that allura red AC has the ability to quench the intrinsic fluorescence of HSA through static quenching. The negative values of the thermodynamic parameters Δ G , Δ H , and Δ S indicated that hydrogen bond and van der Waals forces are dominant in the binding between the food colourant and HSA . The CD and Raman spectra showed that the binding of allura red AC to HSA induces the rearrangement of the carbonyl hydrogen-bonding network of polypeptides, which changes the HSA secondary structure. This colourant is bound to HSA in site I, and the binding mode was further analysed with the use of the CDOCKER algorithm in Discovery Studio.
研究领域:
生物化学
·
食品科学
·
药物化学
✓ 已验证
DOI
高影响因子
TALANTA
·
2017
·
IF 4.162
Quanqing Zhang,Yuanyu Huang,Haofan Sun,Yangyang Gan,Huali Shen,Bin Jia,Pengyuan Yang
Novel 3-D floor-like ordered mesoporous carbon functionalized graphene composites, FLOMC-GO, with high graphitized carbon contents were successfully synthesized using a soft template method. The one-pot sol-gel method was employed to prepare the silica soft template. Then, the sandwich-like composites were further combined together to form a 3-D structure through pre-carbonization and carbonization . During these procedures, the sulphonyl and sulfide bridges were formed by cross-linking processes to connect the phenyl rings. The prepared FLOMC-GO was confirmed to have a large pore volume (1.03 cm 3 g −1 ), high BET surface area (544.99 m 2 g −1 ) and well-ordered mesoporous structure with a narrow pore-size concentrated at 3.74 nm. The content of carbon reached 80% and was highly graphitized. By taking advantage of the interactions between carbon and glycans, FLOMC-GO was utilized to enrich N-linked glycans from OVA and human serum. As expected, excellent size-exclusion was found during the enrichment of N-glycans released from OVA, and 25 N-linked glycans were identified. The intensity of glycans enriched by FLOMC-GO was 7 times to the result of active carbon, while the ratio of OVA digestion to BSA interfering proteins increased to 1:50. Additionally, 31 N-linked glycans in total were enriched from human serum. The relatively easy synthesis as well as ability to enrich N-linked glycans with high selectivity and efficiency makes FLOMC-GO a promising adsorbent material for the discovery of human serum biomarkers for disease diagnosis.
研究领域:
生物材料学
·
糖组学
·
分子诊断
·
纳米医学
·
生物分离技术
✓ 已验证
DOI
高影响因子
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2024
·Vol. 333
·期 4
·2069-2080
·
IF 1.5
Gong Mi,Zhou Xiayu,Yang Xinxu,Grimes Craig A.,Tang Ziwei,Yang Shengyuan,Wang Yue,Xiong Lihao,Song Jiayi,Li Le,Zhen Deshuai
Presented is an infrared-induced sensor constructed of upconversion nanoparticles and DNAzyme for ultra-sensitive, and rapid detection of uranyl ions. The detection method eliminates the use of chemically labeled nucleic acid probes, possesses high selectivity, and a uranyl detection limit of 39 nM. The technique is successfully applied to the detection of uranyl in various water samples as well as human serum, illustrating the excellent sensitivity and specificity of the quantification platform.
研究领域:
分析化学
·
环境科学
·
生物化学
·
纳米技术
·
传感器
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Hao Lu,Yu Yang,Ruijue Chen,Wenyi Huang,Hao Cheng,Xueping Liu,Hongxing Kong,Lijun Li,Jun Feng
In this study, we synthesized gold nanoparticles (AuNPs) in situ on the surface of two-dimensional Cu-TCPP (meso-tetra(4-carboxyphenyl)porphine) metal– organic framework (MOF) nanosheets to produce AuNP@Cu-TCPP nanocomposites as surface-enhanced Raman scattering (SERS) substrates. The SERS signal was enhanced in two ways: aggregation of the AuNPs@Cu-TCPP near the SERS hotspot through the strong adsorption capacity of the MOF, and the synergistic effect between the charge-transfer enhancement of the Cu-TCPP MOF and the electromagnetic enhancement of plasmonic metal AuNPs. The SERS activity of AuNPs@Cu-TCPP was evaluated by the analysis of multiple model analytes; Rhodamine 6G (R6G) and benzidine were detected with detection limits below 10 –13 and 10 –12 mol·L –1 , respectively. We used AuNP@Cu-TCPP as a SERS substrate combined with an enzyme-linked immuno- sorbent assay (ELISA) to detect the tumor marker carboxylesterase 1 (hCE1) in human serum. The SERS signal of benzidine at 1609 cm −1 is linearly correlated with hCE1 in the concentration range of 0.1–120 ng·mL −1 . Owing to their high sensitivity and excellent SERS sensing capability, AuNPs@Cu-TCPP nanocomposites have potential applicability in the analysis of clinical samples.
研究领域:
生物医学工程
·
分析化学
·
肿瘤标志物检测
✓ 已验证
DOI
高影响因子
Current Drug Delivery
·
2022
·Vol. 19
·期 1
·49-63
·
IF 3.758
Haijun Shen,Qianqian Gao,Tingting Liu,Haoran Wang,Ran Zhang,Jie Zhou,Sihui Ding,Yang Ye,Zhenhua Sun
Background: The combination of photothermal therapy (PTT) and chemotherapy has proven to be a promising strategy for cancer treatment. Various nanomaterials have shown great potential in combination therapy, including gold, graphene oxide, iron oxide, and other nanoparticles. However, their undefinable toxicity in vivo greatly slowed down their development for clinical applications. Objective: The present work aimed to develop a multifunctional nanoparticle for chemo-photothermal therapy composed of acknowledged biocompatible materials. Methods: A novel biocompatible nanoparticle (HIT-NPs) was self-assembled through the intrinsic interaction between D-α-tocopherol Succinate (TOS), human serum albumin (HSA) and indocyanine green (ICG). Doxorubicin (DOX) was then loaded due to the ion pairing between DOX and TOS. The feasibility of combined chemo-photothermal therapy induced by DOX-loaded HIT-NPs was carefully evaluated. Results: In vitro, HIT-NPs showed no cytotoxicity on human normal liver cells (HL-7702 cells) but obvious killing effects on murine breast cancer cells (4T1 cells). The combined chemo-photothermal therapeutic effect on 4T1 cells was successfully obtained. DOX-loaded HIT-NPs could effectively accumulate in 4T1 subcutaneous tumors after intravenous injection, and the tumor temperature rapidly increased under laser exposure, indicating the feasibility of PTT in vivo. Conclusion: The self-assembled HIT-NPs could provide a promising platform for combined chemo- photothermal cancer therapy with full biocompatibility.
研究领域:
肿瘤学
·
纳米医学
·
生物材料
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）