JOURNAL OF APPLIED POLYMER SCIENCE
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2025
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IF 2.8
Jinzheng Zhang,Xiaoyu Lei,Jiajing Tang,Qing Zhao,Li Li,Jinlun Feng,Yubao Li,Yi Zuo
To address the challenges associated with bone cement curing in the presence of bleeding, a moisture-tolerant polyurethane-based cement was developed, demonstrating gender-specific therapeutic efficacy when repairing osteoporotic bone defects. The synthesized cements (PHE-1, PHE-2, and PHE-3) incorporated osteoinductive nano-hydroxyapatite (20 wt%) and varying concentrations of enoxacin (EN, 1–3 wt%) as an osteoclast inhibitor. These formulations exhibited moderate curing temperatures (36.8°C–40.7°C), maintained mechanical stability in the presence of blood, and achieved 99% monomer conversion within 240 min via moisture-triggered –NCO reactions. In vitro evaluations showed that PHE-2 effectively inhibited osteoclastogenesis in RAW 264.7 cells while simultaneously promoting osteogenic differentiation in bone marrow-derived mesenchymal stem cells. In vivo studies revealed more efficacious bone healing in ovariectomized rats compared to orchiectomized rats after 8 weeks of implantation. This difference was attributed to estrogen deficiency accelerating the degradation of the bone organic matrix, whereas androgen deficiency impaired biomineralization processes. Further mechanistic analysis indicated that gender-specific differences in bone turnover result from the enhanced matrix degradation induced by estrogen deficiency. Notably, PHE-2 effectively mitigated matrix-related pathologies associated with ovariectomies. This study highlights the importance of gender-specific approaches in managing osteoporotic bone defects and underscores the therapeutic potential of EN-loaded polyurethane cement for treating osteoporotic defects.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2026
·
IF 4.1
Yi Jintao,Fu Nani,Zhong Wenhui,Liu Xia,Zhou Anqi,Li Xun
Amoxicillin (AMO), a penicillin antibiotic, and ciprofloxacin (CIP), a quinolone antibiotic, are widely used to combat bacterial infections and are frequently co-administered to enhance therapeutic efficacy. However, improper use of AMO and CIP may lead to antibiotic residues in the environment, posing serious threats to human health. The visual detection of AMO and CIP remains significant challenges. Herein, the Eu-DPA@CQDs was used for the dual-mode detection of AMO and CIP, which was synthesized by CQDs doped in europium-DPA metal-organic framework. In the colorimetric mode, the catalase-mimicking Eu-DPA@CQDs oxidized TMB to blue oxTMB. Upon addition of AMO, the oxTMB was reduced, causing color fading. Subsequent introduction of CIP restored nanozyme activity, regenerating the blue signal and enabling dual-analyte antibiotic detection. In the fluorescence mode, the Eu-DPA@CQDs probe exhibited dual emission at 440 nm and 613 nm. Upon AMO addition, the fluorescence at both wavelengths was quenched, accompanied by a color change from red to purple. In the presence of CIP, the emission at 440 nm increased and redshifted to 490 nm, while the signal at 613 nm was quenched, resulting in a multicolor transition from red to cyan. The Eu-DPA@CQDs sensor enabled rapid and dual-mode detection of AMO and CIP. The colorimetric method exhibited wide linear ranges of 0.005–25 μM for AMO and 0.007–4.25 μM for CIP, with detection limits of 1.67 nM and 3.75 nM, respectively. A visible color transition from dark blue to colorless was observed with increasing AMO concentration, while the addition of CIP reversed the signal back to dark blue. Similarly, the fluorescence detection achieved wide linear ranges from 0.005 to 30 μM for AMO and 0.005 to 15 μM for CIP, with detection limits as low as 4.74 nM and 3.11 nM, respectively, also within only 1 min. As the concentration of AMO increased, the color shifted from red to purple. In contrast, increasing CIP concentration induced a color transition from red to cyan. These color transitions were both clearly visible to the naked eye. The sensor demonstrated excellent selectivity and anti-interference capability, providing a reliable platform for accurate, sensitive, and quantitative determination of both antibiotics in real samples. Graphical abstract
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MICROCHEMICAL JOURNAL
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2026
·
IF 5.1
Jinli Lin,Xueying Wu,Fangkai Du,Lirong Jiang
The presence of fluoroquinolone antibiotic residues brings significant threats to food safety and human health. This situation has necessitated the development of rapid visual detection methods for identifying these residues in food products. This study constructed a self-calibrating ratiometric fluorescent sensor based on europium and deep eutectic solvent complex (Eu-DES) for the sensitive visual detection of levofloxacin (LEV). By mixing Eu 3+ with the DES (oxalic acid:choline chloride = 1:1) ligand at room temperature, a stable red fluorescence at 623 nm was successfully achieved in the Eu-DES system. Upon the addition of LEV, the Eu-DES induced a red-shift fluorescence of LEV, enabling a self-calibrating ratiometric fluorescent response to LEV with a wide linear range (0.5–100 μM) and a low detection limit (86.50 nM, S/N = 3). Furthermore, integrating Eu-DES into a gel matrix and combining it with smartphone-assisted RGB color analysis enabled quantitative LEV detection through multicolor shifts from red to green. This approach was successfully applied to detect LEV in honey and milk with high sensitivity and good recovery (92.7%–106.4%). The Eu-DES-based detection platform developed in this study provides a powerful tool for on-site LEV detection in actual food samples, promising to advance rapid food safety testing technologies.
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Science China-Life Sciences
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2026
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IF 9.5
Wang Xugang,Zhang Chenxi,Yang Chengjie,Guo Xiaotong,Hu Siyun,Qian Chaonan,Liao Bingrui,Ashraf Usama,Jin Weiwei,Yang Fei,Ouyang Hehao,Liu Yixin,Chen Huanchun,Huang Qiuying,Si Youhui,Zhu Bibo,Cao Shengbo,Ye Jing
Orthoflaviviruses are recognized as significant emerging mosquito-borne pathogens globally. Members of the Japanese encephalitis (JE) serogroup can be distinguished from other orthoflaviviruses by the presence of an additional non-structural protein (NS1’). However, the role of the NS1’ protein in the virus’s life cycle and transmission remains incompletely understood. This study demonstrates that the acquisition of the secretory NS1’ protein from JE serogroup viruses by mosquitoes from infected hosts significantly suppresses the antiviral effect of the RNA interference pathway, thereby facilitating viral infection in mosquitoes. Furthermore, immunization of mice with the NS1’ protein showed considerable efficacy in preventing the transmission of orthoflaviviruses from mice to mosquitoes. These findings indicate that targeting the NS1’ protein may represent a promising strategy for controlling the spread of JE serogroup viruses and other orthoflaviviruses between vertebrate hosts and mosquitoes.
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MICROCHIMICA ACTA
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2026
·Vol. 193
·期 4
·284
·
IF 5.4
Wang Yanbin,Wang Liling
Nanoenzymes have emerged as a powerful alternative to natural enzymes due to their excellent chemical stability and simple synthesis process. In this study, sulfur-doped iron-based nanostructures (FeS 4 -T) were synthesized through a one-step hydrothermal method under mild conditions. FeS 4 -T exhibited strong peroxidase-like activity, and the addition of norfloxacin significantly enhanced the color response of TMB/H 2 O 2 /FeS 4 -T. Thus, a rapid and sensitive method for detecting norfloxacin was developed with a linear range of 0.05–10 μmol·L⁻ 1 and a detection limit of 2 nM. Moreover, various quinolones produced different absorbance changes at 652 nm and 370 nm, forming a unique dual-wavelength signal. Based on these patterns, a colorimetric array was developed to identify seven quinolones and their mixtures. The sulfur-doping strategy proposed provides a novel approach for the rational design of iron-based nanoenzymes and highlights its significant potential in the environmental monitoring of emerging pollutants. Graphical abstract
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2026
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IF 9.6
Cong Yan,Ruyi Li,Ding Yang,Mingchuan Yu,Cong Liu,Hui Li,Yufei Zhou
Graphitic carbon nitride prepared through supramolecular prepolymerization and molten-salt strategies has shown strong potential in photocatalytic hydrogen evolution and pollutant degradation. In this work, we integrate these two approaches to construct single-atom-anchored carbon nitride (NaK-CN) and demonstrate that precise calcination temperature control enables divergent photocatalytic functionalities. Aberration-corrected high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM) and X-ray absorption near-edge structure (XANES) analyses were employed to elucidate the structural features of the materials. The results reveal that temperature-dependent tuning of defect concentration, coordination environment, and crystallinity effectively regulates charge separation behavior and reaction pathways. Consequently, NaK-CN-500 achieves a high hydrogen evolution rate of 12.81 mmol h −1 g −1 catalyst , while NaK-CN-550 delivers a markedly enhanced apparent rate constant of 0.431 min −1 for enoxacin degradation. This study supplements a versatile synthesis platform for tailoring carbon nitride frameworks and provides mechanistic insight for designing CN-based photocatalysts optimized for energy conversion and environmental remediation.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2026
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IF 9.6
Zhiming Chen,Jiabin Chen,Haoming Bao,Kaitao Liu,Pan Zeng,Fan Yang,Wei Cao,Dongqi Yang,An Cao,Tao Zhang,Yue Li
Surface-enhanced Raman scattering (SERS) has been one of the efficient strategies for trace molecular analysis, but the traditional substrates with sharp tips cannot effectively detecting the molecular isomers. Here, we develop a facile way to fabricate large-area, reproducible Ag/Au nanocavity arrays by the combination of microsphere-templated soft lithography and self-assembly. Compared to the flat and convex substrate, the Ag/Au units arranged on the concave surface exhibit a high-density of hotspots via spatial light confinement and multi-reflection-enhanced electromagnetic coupling, which enables highly sensitive detection of probe molecules down to10 −12 M. Moreover, by integrating machine learning algorithms, the test set shows a coefficient of determination (R 2 ) of 0.9896, achieving advanced training performance for classifying four structurally analogous quinolone antibiotics (ciprofloxacin, enrofloxacin, danofloxacin, and enoxacin) with a quantitative prediction of 10 −10 M. This work demonstrates a robust pathway for the controllable fabrication of high-performance SERS substrates for the development of intelligent, quantitative, and on-site sensing platforms in complex practical scenarios. Download: Download high-res image (179KB) Download: Download full-size image
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FOOD CHEMISTRY
·
2025
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IF 9.8
Guangxin Yang,Jingru Zhang,Junyu Zhang,Peng Wang,Wei Xia,Jun Wang,Xiaosheng Shen,Cong Kong
Antibiotic use in aquaculture leads residues in aquatic products, contributing to antimicrobial resistance and potential human toxicity. Complex sample matrices hinder detection. This study uses wolfberry biomass waste-derived biochar as a novel solid-phase extraction (SPE) sorbent to detect 26 antibiotic residues (14 fluoroquinolones, 12 sulfonamides) in aquatic products, effectively repurposing agricultural waste into a high-performance, eco-friendly material. The mesoporous structure of the biochar enables rapid diffusion of antibiotics into its interior, facilitating highly efficient adsorption. Adsorption kinetics follow a pseudo-first-order model, reaching equilibrium in 60 min. The superior adsorption performance enables the prepared biochar to serve as an SPE sorbent for drug residue analysis with stable performance over at least three reuse cycles. The SPE was used for real samples analysis based on HPLC-HRMS, and the detection limits was 1–4.5 μg·kg −1 , recoveries was 75–119 %. Validation against the national standard GB 31658.17–2021 confirms method practicality and reliability.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Yanning Zheng,Dankui Liao,Bimei Xie,Jinlei Sun,Jianhua Sun,Zhangfa Tong,Guangzhi Zhou
Effective removal of antibiotic residues from water is crucial for maintaining balance and cleanliness in the ecological environment. To achieve this, we conducted a study on the development of a highly efficient catalyst from the in-situ growth of Fe 3 O 4 nanoparticles on nitrogen-doped hollow porous carbon spheres (Fe 3 O 4 @N-HollCS). We controlled the in-situ growth time to optimize its performance. Material characterization and theoretical calculations revealed that the improved catalytic performance of Fe 3 O 4 @N-HollCS is due to electron transfer from the graphitic-N in N-HollCS to the catalytic interface of Fe 3 O 4 nanoparticles. Fe 3 O 4 @N-HollCS, with an ordered mesoporous structure and high specific surface area, had remarkable adsorption capacity for NOR antibiotics, with an enrichment efficiency exceeding 60% within 10 min. Moreover, the Fe 3 O 4 @N-HollCS degraded more than 90% of fluoroquinolone antibiotics, especially norfloxacin (NOR), which was eliminated within 60 min. The Fe 3 O 4 @N-HollCS also exhibited stable catalytic recycling ability within 10 magnetic separations. Fukui function predicted the sensitive sites of NOR, and we proposed potential degradation pathways. Ecological structure activity relationship model evaluation showed that the intermediate produced by Fe 3 O 4 @N-HollCS degradation of NOR has low levels of ecotoxicity. Overall, Fe 3 O 4 @N-HollCS catalysts have great potential for antibiotic wastewater treatment.
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MICROCHIMICA ACTA
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2022
·Vol. 190
·期 1
·1-9
·
IF 6.408
Wang Jiarui,Feng Juanjuan,Sun Min,Lian Yujing,Wang Minglin,Qiao Luqin
Sulfonic acid-functionalized covalent organic frameworks (COF-SO 3 ) as a coating of stir bar sorptive extraction (SBSE) for capturing three fluoroquinolones from milk have been developed. The COF-SO 3 material was characterized by X-ray diffraction, scanning electron microscopy, X-ray photoelectron spectroscopy, and infrared spectroscopy. Milk without any typical treatments like protein precipitation and defatting was only diluted five times with water for test. Combined with high-performance liquid chromatography (HPLC), a SBSE-HPLC method was established for detecting fluoroquinolones in milk samples. The corresponding wide linear ranges (4.00–500.0 µg L −1 ), low detection limits (1.20–2.62 µg L −1 ), good test repeatability (RSD < 5.2%), and acceptable enrichment factors (56.2–61.5) were implemented for three fluoroquinolones. The analytical method was applied to determine trace targets and provided satisfactory results. Furthermore, the research displayed satisfied reproducibility for bar-to-bar (RSD < 6.5%) and batch-to-batch (RSD < 8.6%) tests. Graphical abstract
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MOLECULES
·
2022
·Vol. 27
·期 24
·8764
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IF 4.927
Panxue Wang,Li Wang,Cen Li,Xiang Li,Guoliang Li
Reliable detection and quantification of antibiotic residues in food using surface-enhanced Raman spectroscopy remain challenging, since the intensities of SERS signals are vulnerable to matrix and experimental factors. In this work, a ratiometric SERS aptasensor using 6-Carboxyl-X-Rhodamine (ROX)-labeled aptamers and 4-mercaptobenzonitrile (4-MBN)-functionalized gold nanoparticles (Au NPs) as SERS probes was established for the reliable and rapid detection and quantification of enrofloxacin. In the presence of enrofloxacin, the conformational transform of aptamers took place, and the distance between ROX and Au NP increased, which resulted in a decrease in the SERS signal intensity of ROX. Meanwhile, the intensity of the SERS signal of 4-MBN was used as an internal standard. Reliable determination of enrofloxacin was realized using the ratio of the SERS signal intensities of ROX to 4-MBN. Under optimal conditions, the developed ratiometric SERS aptasensor provided a wide linear range from 5 nM to 1 µM, with a correlation coefficient (R2) of 0.98 and a limit of detection (LOD) of 0.12 nM (0.043 ppb). In addition, the developed ratiometric SERS aptasensor was successfully applied for the determination of enrofloxacin in fish and chicken meat, with recovery values of 93.6–112.0%. Therefore, the established ratiometric SERS aptasensor is sensitive, reliable, time-efficient, and has the potential to be applied in the on-site detection of enrofloxacin in complex matrices.Keywords:enrofloxacin;detection;SERS aptasensor;ratiometric;portable Raman spectrometer;food safety
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Chinese Journal of Chromatography
·
2022
·Vol. 40
·期 5
·433-442
·
IF 0
Yangjie LI,Jiaying HUANG,Jihui FANG,Zhiye HUANG
A high throughput screening method based on ultra performance liquid chromatography-quadrupole-time-of-flight high resolution mass spectrometry (UPLC-Q-TOF HRMS) was developed for the simultaneous and rapid confirmation of 73 prohibited compounds in cosmetics. The sample was dispersed in a saturated sodium chloride solution and ultrasonically extracted using acetonitrile containing 0.2% (v/v) formic acid. The resultant solution was centrifuged and then cleaned using dispersive solid phase extraction using a primary secondary amine (PSA) sorbent. The purified solution was centrifuged, and the supernatant was filtered through a 0.22 μm membrane before determination. The optimal pretreatment method was determined by comparing the recovery rates obtained using different extraction solvents and different amounts of purifying agents. The chromatographic separation conditions and mass spectrometry scanning mode were also optimized. Chromatographic separation was performed on an Acquity UPLC HSS T3 column (100 mm×2.1 mm, 1.8 μm) with gradient elution using 0.1% (v/v) formic acid aqueous solution and methanol as mobile phases. The eluent from the column was further detected using Q-TOF HRMS with the high resolution multiple reaction monitoring (MRM HR) scanning mode. Retention time, precise mass of parent ion, isotope abundance ratio, and precise mass of fragment ions were the parameters considered for rapid untargeted screening and confirmation. The matrix effects of water- and cream-based cosmetics were investigated. The matrix effects could be addressed using the matrix matched standard curve method. The correlation coefficients for the 73 prohibited compounds were all >0.99 in the corresponding linear concentration range. The limits of detection (LODs) were in the range of 5-150 μg/kg, and the limits of quantification (LOQs) were in the range of 15-450 μg/kg. Average recoveries were in the range of 60.3%-130.3% at three spiked levels, and the intra-day and inter-day precisions were 0.8%-10.0% ( n =6) and 1.1%-15.0% ( n =3), respectively. A total of 692 cosmetics samples were screened; 16 positive samples were detected, namely, sulfamethoxazole, meprednisone, lincomycin, 4-acetamidophenol, trimethoprim, alfacalcidol, betamethasone 17-valerate, brimonidine, chloramphenicol, chlorpheniramine, clobetasol propionate, crotamiton, econazole, ketoconazole, prednisone 21-acetate, and prednisone, with content in the range of 0.5-1136.1 mg/kg. The optimized method is accurate, fast, and simple, and it is suitable for the routine detection and rapid screening of common prohibited compounds in cosmetics. In addition, a screening and confirmation library was established for the 650 prohibited compounds using SCIEX OS and Library View software, using information-dependent acquisition (IDA)-MS/MS mode for MS data acquisition. The database contains multiple types of information, including formulas, theoretical exact mass, retention time, precise mass of parent ion, isotope abundance ratio, and fragment ion distribution. The library can be used for the simultaneous and rapid confirmation of prohibited compounds in cosmetics.
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危险品化学品经营许可证（带存储）