ANALYTICA CHIMICA ACTA
·
2026
·
IF 6.1
Zhongjie Wang,Yuhao Wang,Yang Guo,Zhong-Xia Wang,Wei Wang,Hang Gao
Ensuring appropriate quercetin (Qn) levels in food is essential for food safety and human health. In this work, we developed a sensitive electrochemiluminescence (ECL) sensing platform for Qn analysis, utilizing aggregation-induced ECL (AIECL)-active polymer dots (Pdots) as the emitter. Benefiting from the restriction of intermolecular motions in the aggregated states, the prepared Pdots exhibit efficient ECL emission. Upon Qn introduction, the ECL signal was effectively quenched via a dual-path electron and/or energy transfer mechanism. This “signal on-off” strategy enables ultrasensitive and highly selective Qn detection, achieving a broad linear range from 0.1 nM to 1.0 mM with a low detection limit of 41 pM. The sensor was successfully applied to determine Qn in various real food samples with satisfactory recoveries. This work not only offers a reliable method for Qn monitoring but also highlights its promise for advancing food safety.
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ACS Nano
·
2025
·
IF 16
Yin Zhou,Yutong Chen,Wentao Zhao,Jiafeng Wang,Yi Chen,Haobo Wen,Yiyan He,Ning Li,Hongli Mao,Yuwen Cui,Zhongwei Gu
Intracellular bacterial infections are challenging due to immune evasion and antibiotic resistance, especially within macrophages harboring dormant bacteria. Here, a synergistic antibacterial strategy is presented using the PG3M@GaPP nanoflowers, which integrate immune modulation, iron metabolism disruption, and antibacterial photodynamic therapy (APDT) to eliminate intracellular bacteria. This nanoflower encapsulates a gallium–protoporphyrin IX complex (GaPP) within mannose-functionalized poly-l-lysine dendrimers (PG3M), enabling targeted delivery to macrophages via mannose receptor recognition. PG3M@GaPP promotes macrophage polarization to the anti-inflammatory M2 phenotype, enhancing immune modulation and bacterial uptake. The platform’s positive charge facilitates endosomal escape, releasing GaPP in the acidic intracellular environment, where free iron ions compete with gallium ions to form the iron–protoporphyrin IX complex (FePP). This disrupts the gallium/iron ion balance, enhancing the Trojan horse effect of gallium ions and inducing iron metabolism-dependent bacterial death. Additionally, laser activation of GaPP generates reactive oxygen species (ROS), further amplifying bacterial killing via APDT. In vitro and in vivo experiments show that PG3M@GaPP outperforms both free GaPP and commercial antibiotics in eliminating intracellular bacteria. These nanoflowers offers an alternative, nonantibiotic approach to combat intracellular infections, addressing drug resistance and providing a promising platform for antibacterial therapies.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Yongda Wang,Zheng Hu,Jia Li,Yunhan Zhao,Meng Kou,Feng Qin,Zhiguo Zhang
A dual-excitation laser modulation technique for the sensitive measurement of photosensitizers in tumor is proposed. The technique is based on the difference in optical absorption properties of photosensitizers and biological tissues at 405 nm and 450 nm. The 405 nm and 450 nm laser beams, with the same modulation frequency and 180° phase difference, were coupled into a single fiber simultaneously. A periodic signal with a frequency of 1 kHz was generated in alternating excitation, and was processed by phase sensitive detectors. The weak photosensitizer fluorescence masked by tissue autofluorescence can be effectively separated through the proposed method, so that the final signal intensity is only related to the concentration of photosensitizer. The method with a sensitivity of 0.22 mL/ng was determined by measuring the low concentration of protoporphyrin IX (PpIX) solution, and the detection limit of instrument reached 0.5 ng/mL. The puncturable optical probe with a diameter of 675 µm ensures that PpIX in can be detected with spatial resolution, which was performed in the measurement of PpIX in tumor bearing nude mice .
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Jie An,Xinyi He,Huizhu Ma,Yanglei Li,Yayuan Li,Xinyu Zhang,Qizhi Shuai,YinMeng Wang,Wen Liu,Weihua Li,Hongliang Wang,Zhifang Wu,Sijin Li
Radionuclide therapy (RNT) is an effective method for the clinical precise treatment of cancer. However, the uneven dose distribution and rapid metabolism of nuclides limit the effective killing of tumors. To overcome the limitations of radionuclide therapeutic approaches, combining different therapeutic strategies to treat cancer has manifested great promise in basic and clinical research. Here, a new combination therapy strategy was developed to combine radionuclide therapy, sonodynamic therapy and photothermal therapy (RNT-SDT-PTT) under radionuclide imaging guided achieve highly effective combination therapy. We prepared a polydopamine-modified Au nanostar (AN), then loaded with the acoustic sensitizer protoporphyrin (IX) and labeled with diagnostic ( 99m Tc) or therapeutic ( 131 I) radionuclides ( 131 I/ 99m [email protected] /IX) for the precise diagnosis and treatment of pancreatic cancer. After intratumor administration, single photon emission computed tomography imaging showed that the nanocarriers were mostly retained in the tumor compared to free radionuclide. As well as using near-infrared light to trigger PTT and ultrasound with high penetration depth to activate IX to generate reactive oxygen species achieved SDT of tumor. The ultimate significantly improved the inhibitory effects by the RNT-SDT-PTT combined therapy for pancreatic cancer. Therefore, this study proposes an effective radionuclide combination therapy regimen consisting of three widely used treatments, offering promising prospects for the future of oncology.
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2022
·Vol. 140
·期 7
·e53485
·
IF 3.057
Jiahao Shi,Zhengnan Lu,Yan Pan,Yang Sheng,Yixin Sun,Linhong Deng,Mark Bradley,Le Zhou,Rong Zhang
Protoporphyrin IX (PpIX) is a photosensitizer used in photodynamic therapy, however, its poor aqueous solubility always presents challenges and limits its application. To solve these problems water-soluble derivatives of PpIX were synthesized by esterification with polyethylene glycol, and polymerization of the PpIX diol with hexamethylene diisocyanate to form a water-soluble polyurethane. The polyurethane PpIX derivatives were characterized and showed high levels of singlet oxygen generation. The photodynamic antibacterial activity of the PpIX derivatives were explored on Escherichia coli , Staphylococcus aureus and methicillin-resistant Staphylococcus aureus indicated that 100% antibacterial killing was achieved with a concentration of 40 μM upon 405 nm irradiation for 5 min. The polyurethane showed high biocompatibility in the dark with a variety of cell lines, but when exposed to 405 nm light (for 5 min) cytotoxic singlet oxygen was generated, resulting in cell death. Importantly, this was also possible with 635 nm illumination. In summary this novel protoporphyrin polyurethane overcomes the issues of solubility, and offers enhanced killing (singlet oxygen) generation with the potential to be used in photodynamic therapy for both antibacterial and anticancer treatment.
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ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Xiaoying Wang,Tao Gao,Jin Zhu,Shipeng Long,Songyan Zhao,Li Yuan,Zhaoxia Wang
Hematogenous metastasis is the main route of cancer spreading, causing majority death of cancer patients. During this process, platelets in the blood are found increasingly essential to promote hematogenous metastasis by forming platelet-interacted circulating tumor cells (CTCs). Hence, we aim to fabricate an integrated method for the availability of capture and detection of such invasive CTCs. Specifically, a new form of channeled and conductive three-dimensional (3D) electrode is constructed by modifying a conductive layer and capture antibody on the templated and channeled poly(dimethylsiloxane) scaffold. The modified antibody enables the capture of the platelet-interacted CTC hybrid, while the conductive layer significantly facilitates electron transfer from electro-active signal molecules that are targeting platelets. Therefore, sensitive electrochemical detection of platelet-interacted CTCs has been realized. Efficient capture and sensitive detection have been demonstrated by this work. Additionally, dynamic analysis of patients’ CTCs has also been conducted to provide accurate information about disease assessment and efficacy evaluation. The cut-off line was set as 5.15 nA based on the sample signals from healthy volunteers. Thus, stage III cancer patients with high risk of hematogenous metastasis have been identified. Together, this work shows the development of a new strategy for simultaneous capture and detection of the invasive CTC subtype form patient blood, which favors precise monitoring of hematogenous metastasis.
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Acta Biomaterialia
·
2023
·
IF 9.7
Kang Song,Guobo Chen,Zongyan He,Jing Shen,Jing Ping,Yuhao Li,Lulu Zheng,Yuqing Miao,Dawei Zhang
Decreasing the scavenging capacity of reactive oxygen species (ROS) and enhancing ROS production are the two principal objectives in the development of novel sonosensitizers for sonodynamic therapy (SDT). Herein, we designed a protoporphyrin-sensitized bismuth-based semiconductor (P-NBOF) as a sonosensitizer to generate ROS and synergistically depleted glutathione for enhanced SDT against tumors. The bismuth-based nanomaterial (NBOF) is a wide-bandgap semiconductor. Sensitization by protoporphyrin made it easier to excite electrons under ultrasonic stimulation, and the energy of the lowest unoccupied electron orbital in protoporphyrin was higher than the conduction-band energy of NBOF. Under ultrasound excitation, the excited electrons in the protoporphyrin were injected into the conduction band of the NBOF, increasing its reducing ability leading to the production of more superoxide anion radicals and also helping to increase the charge separation of protoporphyrin leading to the production of more singlet oxygen. Meanwhile, P-NBOF continuously depleted glutathione, which was not only conducive to breaking the redox balance of the tumor microenvironment to enhance the therapeutic efficacy of SDT, but also promoted its degradation and metabolism. The construction of this P-NBOF sonosensitizer thus provided an effective strategy to enhance SDT for tumors. Statement of significance To enhance the efficacy of sonodynamic tumor therapy, we developed a degradable protoporphyrin-sensitized bismuth-based nano-semiconductor (P-NBOF) by optimizing the band structure and glutathione-depletion ability. Protoporphyrin in P-NBOF under excitation preferentially generates free electrons, which are then injected into the conduction band of NBOF, improving the reducing ability of NBOF and promoting the separation of electron-hole pairs, thereby enhancing the production capacity of reactive oxygen species. Furthermore, P-NBOF can deplete glutathione, reduce the scavenging of reactive oxygen species, and reactivate and amplify the effect of sonodynamic therapy. The construction of the nanotherapeutic platform provides an option for enhancing sonodynamic therapy.
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Environmental Technology & Innovation
·
2022
·
IF 7.758
Xijuan Chao,Dezhi Yao,Chuxuan Chen,Chengfei Zhang
Hemin, an iron (III) complex of protoporphyrin IX dissociated from met Hb, exhibits many biological functions. The excess of hemin can be extremely toxic, such as permeabilizing cellular membranes and oxidizing proteins, lipids and nucleic acids. Therefore, it is of great significance to achieve hemin detection. Here, a simple and environmental-friendly fluorescence-based approach for hemin detection is reported by using synthesized dicyanomethylene-4H-pyrans-morpholine ( DCM-ML ) as a fluorescent signal reporter molecule in the presence of human serum albumin (HSA). DCM-ML is non-fluorescent in aqueous solution, but emits 630 nm fluorescence signal with HSA. Upon hemin addition, the specific competitive-binding towards HSA results in the release of DCM-ML reporter, accompanied by a gradual decrease of fluorescence intensity of the DCM-ML /HSA system. Importantly, the fluorescence intensity at 630 nm exhibits a good linear relationship ( R 2 > 0.99) with the added concentration of hemin at the range of 0– 2 . 8 μ m ol/L, giving a quite low detection limit of 9.84 nmol/L in PBS buffer. The DCM-ML /HSA system possesses strong anti-interference capacity against other biomolecules or ions, and fast response time (30 s) for hemin and a wide pH range of 7–10 is generally suitable for the detection system. At last, the applications of DCM-ML /HSA system for hemin detection in environmental and biological systems are successfully demonstrated, indicating its practical application potential in the future.
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Journal of Materials Chemistry A
·
2022
·Vol. 11
·期 4
·1829-1840
·
IF 14.511
Yan-Li Ji,Kai Zhang,Jin-Lin Wang,Zhuang-Zhuang Liu,Xin Wen,Cong-Jie Gao
Porous organic polymer (POP) membranes show great promise for the precise separation of organic molecules and salt ions due to their high porosity, tunable pore aperture, and robust porous structure. However, their application is hindered by the poor processability of POPs and complicated and uncontrollable membrane preparation. Herein, we report a nanoparticle-polymer reticulation (NPR) strategy by bridging POP nanoparticles for the fabrication of POP nanochannel membranes with a relatively large area (>700 cm2). A new type of water dispersible and processible triazine porous organic polymer (TPOP) nanoparticles was synthesized via the facile and straightforward Debus–Radziszewski reaction. Chitosan (CS) macromolecules as the bridge tied the self-assembled TPOP nanoparticles into a thin film via in situ chemical cross-linking on a polymeric porous substrate, wherein the resultant film thickness, pore size and separation performance could be conveniently tuned. The NPR strategy enabled the construction of an ultrathin and continuous nanochannel TPOP-CSM membrane, presenting both a high water permeance of ∼130 L m−2 h−1 bar−1 and dye/salt selectivity of ∼35, which is one order of magnitude higher than that of conventional polymeric nanofiltration membranes. Meanwhile, the membrane exhibited good fouling resistance and antibacterial properties in an aqueous environment. Our strategy may provide a paradigm shift in engineering POP nanomaterials and constructing next-generation advanced separation membranes.
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VETERINARY PARASITOLOGY
·
2022
·
IF 2.821
Xiaoxiao Qi,Huan Li,Bohan Wang,Jiali Meng,Xu Wang,Weiwei Sun,Baoliang Pan
Dermanyssus gallinae , also known as the poultry red mite (PRM), is one of widespread ectoparasite in the poultry industry worldwide, causing direct and indirect detriments to poultry as well as substantial financial losses. Novel control methods are urgently needed to improve the current acaricide-based control of D. gallinae . The control approach based on arrestment pheromone is environment-friendly but the related research is limited in PRMs. In the present study we found two compounds from the mite feces acting as arrestment pheromones of D. gallinae , which could lead to mite arrestment upon contact. One is guanine, which was also found in unfed female mites’ acid-saline extract. The other is hematin. Moreover, it was found that the ferric ion of hematin played a pivotal role in stimulating the arrestment of mites. Finally, it was found the combination of guanine or/and hematin plus cypermethrin led to significantly improved mite-killing performance compared with cypermethrin, showing a promising potential of novel control method based on the arrestment pheromone.
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JOURNAL OF PHYSICAL CHEMISTRY B
·
2023
·
IF 3.3
Lu Li,Meng Kou,Yongda Wang,Feng Qin,Hua Zhao,Zhiguo Zhang
The oxygen-varying correlated fluorescence provides an effective strategy to gain the quenching constant KSV for a non-phosphorescent system. Owing to the absence of detectable phosphorescence emission in general optical spectroscopy, the facticity of this method has never been verified. Here, the correct way to determine KSV by oxygen-varying correlated fluorescence was systematically studied in detail. We selected gadolinium protoporphyrin IX (Gd-PpIX) to establish a fluorescence and phosphorescence dual emission system. Then, KSV of Gd-PpIX can be obtained by the change of fluorescence intensity (IF) with oxygen concentration using the method of correlated fluorescence at intense pump power; meanwhile, the value can also be determined from the relationship of the phosphorescence intensity ratio and oxygen content by the Stern–Volmer equation under relatively low power density. It was found that the KSV values obtained by the above two methods were 12.2(1) and 11.9(7) kPa–1, respectively. Our results successfully verified the accuracy of oxygen-varying correlated fluorescence for determining the KSV by the phosphorescence property and fluorescence saturation of Gd-PpIX.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Liu Wang,Fang He,Xueyun Chen,Kaiyu He,Linlin Bai,Qiang Wang,Fang Zhang,Xiahong Xu
CRISPR/Cas12a, as a powerful and programmable biosensing tool, has brought great convenience in visual detection of the target in a sequence-specific mode. Though fluorescent output is the predominant way of present visual methods, this strategy highly depends on cleaving fluorophore-/quencher-labeled oligonucleotides . Few research focuses on realizing fluorescent visual detection without DNA-labeling. Herein, we have proposed a CRISPR/Cas12a-based label-free fluorescent visual detection strategy. The basic principle of this strategy is that G-quadruplex can enhance the fluorescent emission of fluorescent ligands while dsDNA target-activated Cas12a make them impotent by disrupting the higher-ordered structure. After comparing four kinds of G-quadruplex-specific ligands, we selected Thioflavin T (ThT) to achieve the visual observation goal. The application feasibility was confirmed by combining with loop-mediated isothermal amplification (LAMP) for detection of Vibrio parahaemolyticus ( V. parahaemolyticus ), and it demonstrated a high sensitivity (1.36 × 10 2 copies) and specificity (among 12 kinds of bacteria). The method displayed similar performance as the real-time PCR for detection of real samples yet had lower requirement on the instrument. The signal output format could also distinguish Salmo salar from other seven kinds of sequence-similar Salmonidae. The findings and strategy proposed in this manuscript will expand the application of CRISPR/Cas12a-based label-free fluorescent analysis.
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