FOOD CHEMISTRY
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2026
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IF 10.4
Bo Zhao,Xiuxian Mo,Jiayao Wang,Akang Dan,Tong Chen,Dan Ouyang,Xiaopeng Hu
Dual-mode sensor integrates fluorescence/colorimetric detection for cross-validated acetamiprid monitoring. • Synergistic recognition by MIP and aptamer enhances specificity in complex food matrices. • The MIL-88B (Fe) was used as a dual-signal probe triggered by PPi. • The integration of smartphone-based colorimetric readout and machine learning enhances field applicability. • Achieves ultra-sensitive detection with high recovery in real samples. This work presents a smartphone-integrated, fluorescence/colorimetric dual-mode sensing platform that combines fluorescence and colorimetric detection with support vector regression (SVR) for monitoring of acetamiprid in food. The system employs a sandwich-structured mechanism using MIPs/Fe 3 O 4 as capture probes and aptamer-functionalized MIL-88B(Fe) as bifunctional signal probes. Uniquely, pyrophosphate (PPi) triggers the disassembly of MIL-88B(Fe), synchronously releasing fluorescent 2-aminoterephthalic acid ligands and Fe 3+ ions, which subsequently catalyze Prussian blue formation for colorimetric readout. Furthermore, the integration of smartphone imaging and a SVR mode allows for intelligent translation of RGB color features into precise acetamiprid concentrations, facilitating rapid analysis. The biosensor demonstrates exceptional performance with detection limits of 41.0 pg mL −1 (fluorescence) and 45.0 pg mL −1 (colorimetric), maintaining high specificity in complex matrices. Successful application in fruit and vegetable extracts confirmed its practical utility, offering a sensitive, cross-validation, and field-deployable strategy for food safety monitoring. Download: Download high-res image (176KB) Download: Download full-size image
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JOURNAL OF HAZARDOUS MATERIALS
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2026
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IF 10.6
Ziyan Cheng,Xinru Wang,Zhimin Yang,Jingtong Yu,Nan Zhao,Chenyang Ji,Quan Zhang,Meirong Zhao
Neonicotinoid insecticides (NNIs) are ubiquitously detected in the environment, yet their comparative neurotoxicity and mechanisms remain insufficiently characterized. This study systematically evaluated the neurotoxic potential of eight common NNIs (imidacloprid, acetamiprid, thiacloprid, thiamethoxam, clothianidin, flonicamid, sulfoxaflor, and imidaclothiz) using human SH-SY5Y cells. Results demonstrated that all tested NNIs significantly reduced cell viability. Mechanistically, NNI exposure triggered excessive reactive oxygen species (ROS) production, inhibited superoxide dismutase (SOD) activity, and elevated malondialdehyde (MDA) levels, indicating severe oxidative stress. Notably, Exposure to NNIs caused a dissipation of mitochondrial membrane potential (MMP) and profoundly disrupted mitochondrial bioenergetics. Analysis of energy metabolism revealed that NNIs suppressed both the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), suggesting an impairment of oxidative phosphorylation and glycolysis. These findings suggested that NNIs induced neurotoxicity through coordinated oxidative stress and mitochondrial dysfunction, resulting in global bioenergetic suppression characterized by concurrent impairment of oxidative phosphorylation and glycolysis. This study provides critical toxicological evidence for the environmental risk assessment of NNIs and underscores their potential impact on human neurological health.
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Foods
·
2026
·Vol. 15
·期 15
·2598
·
IF 6
Qingqing Li,Xuecheng Zhang,Shurun Chen,Bowen Guo,Qingwen Cao,Xinyue Feng,Xiaoping Yu,Xiaoyu Jia,Biao Zhang,Hongqing Wang
Acetamiprid (ACE) is a widely used neonicotinoid insecticide with potential health risks due to its persistence in the environment and bioaccumulation in plant-based foods. In this study, an improved dual-mode lateral flow immunochromatography assay (DMLFIA) was established for the sensitive detection of ACE residues in plant-based foods using Au@Ag@Pt NPs as dual-function signal probes. In the colorimetric mode (Mode 1), with the inhibition rate as the vertical coordinate and the ACE concentrations as the horizontal coordinate, the linear detection range of the colorimetric mode was 0.1–3000 μg/L with the detection limit of 0.08 μg/L. In the photothermal mode (Mode 2), the temperature values obtained via smartphones integrated thermal imager showed a linear relationship with 0.1–3000 μg/L of ACE, and the detection limit is 0.1 μg/L. The cross-reactivity rate of the developed DMLFIA with other structural analogs and functional substances was less than 0.39%. The recovery rate of the developed DMLFIA used in spiked plant-based foods was 81.1–99.4%, with relative standard deviations (RSD) of 1.3–10.7%. The detection results of this constructed DMLFIA showed good consistency with those obtained by HPLC and ELISA. The constructed DMLFIA provides a selective, sensitive and rapid detection tool for ACE in different scenarios in plant-based foods.
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ANALYTICA CHIMICA ACTA
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2026
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IF 6.1
Saqib Farooq,Jie Zhang,Jia Xie,Jie Yuan,Xujiang Yang,Xiaoting Su,Weiguo Miao,Yongmei Fan
This study presents a novel analytical method based on molecularly imprinted polymers (MIPs) synthesized using an eco-friendly β-CD-DES functional monomer. These MIPs were developed for the selective extraction of acetamiprid (ACT) and thiamethoxam (TMX). The adsorption affinity analysis revealed rapid adsorption within 15 min, achieving 73% uptake in the first 5 min, with high adsorption capacities of 27.38 mg/g for ACT and 19.86 mg/g for TMX, and also poses strong selectivity towards both. Extraction parameters were optimized, resulting in high recovery rates in spiked fruit samples analyzed by high-performance liquid chromatography. The determined limits of quantification were 0.01 mg/kg (each), and the limits of detection were 0.002 and 0.005 mg/kg (for both ACT & TMX). The method showed low relative standard deviations (RSDs <5.84%) and high selectivity for both ACT & TMX. Additionally, the MIPs retained recovery efficiency above 90% for four reuse cycles before a sharp decline. This study highlights a simple, reliable, and environmentally sustainable method for trace-level extraction of both ACT & TMX from fruits.
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Environmental Science-Nano
·
2026
·
IF 5.6
Fengjiao He,Linpin Luo,Si Li,Xin Liu,Heng Wang,Nannan Qiu,Wentao Zhang,Yongning Wu,Yizhong Shen
Given the serious harm that microcystins (MCs) cause to public health and ecosystems (e.g., freshwater systems), there is a great need for an onsite MCs environmental assay that is cost-effective, easy-to-use, reliable and precise. Regarding this point, we here proposed a molecular imprinting-based selectively regulated laccase-like copper-doped carbon dot nanozyme (MIP@Cu-CD) to engineer a smartphone-assisted portable device, as a proof of concept, yielding the onsite visual colorimetric/fluorescence imaging-driven precise and reliable detection of MC-LR (a representative MCs) in real environmental waters. The detection mechanism was derived from the combination of MC-LR with its recognizable molecularly imprinted cavity, blocking the laccase-like activity-triggered MIP@Cu-CD to oxidize 2,4-dichlorophenol (2,4-DP) into a red quinoneimine product in the presence of 4-aminophenol (4-AP). At this moment, a decrease in colorimetric absorbance at 510 nm and a recovery of intrinsic blue fluorescence at 420 nm were observed in the MIP@Cu-CD + 2,4-DP + 4-AP system, with an increase in MC-LR concentration. On this basis, the system of MIP@Cu-CD + 2,4-DP + 4-AP was integrated with a home-made smartphone-assisted portable device to perform the onsite visual colorimetric/fluorescence images-based RGB detection of MC-LR in tap water, urban sewage, and lake water, with the detection limits of 0.227 μg/L and 0.101 μg/L in colorimetric and fluorescence ways, respectively. Moreover, the reliability was also confirmed by the liquid chromatography-mass spectrometry (LC-MS) method. This work provides a feasible approach to utilize molecular imprinting-based laccase-like nanozyme strategy to integrate portable devices for realizing the on-demand onsite detection of MCs in environmental waters.
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JOURNAL OF MOLECULAR STRUCTURE
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2026
·
IF 4.9
Junzhi Ni,Dian Lei
Chitosan/thiourea nanoprobes enabled selective chlorpyrifos sensing. • Heteroatom-rich carbon surfaces supported molecular recognition. • Static quenching dominated with partial inner-filter contribution. • Detection was linear from 0.06 to 50 μg L⁻¹. • Real samples showed 91.3-97.1% recovery and HPLC-UV agreement. Pesticide residues in soil-water-plant interfaces pose persistent risks to ecosystem recovery, yet rapid sensing platforms that combine sustainable material design, structural interpretability, and matrix-tolerant analytical performance remain limited. Herein, biopolymer-derived fluorescent nanoprobes were engineered from chitosan and thiourea through controlled hydrothermal conversion for selective chlorpyrifos monitoring in restoration-relevant environmental matrices. The nanoprobes exhibited quasi-spherical morphology with an average diameter of 8.5 ± 2.1 nm, amorphous/turbostratic carbon organization, defect-rich aromatic domains, and oxygen-, nitrogen-, and sulfur-containing surface functionalities. These structural features generated blue fluorescence at 445 nm under 340 nm excitation, a quantum yield of 16.8%, nanosecond lifetime behavior, and excellent stability over a wide pH range, ionic strength, storage, and irradiation conditions. Chlorpyrifos recognition was mediated by cooperative hydrogen bonding, donor-acceptor interaction, electrostatic/dipole-assisted association, and π-associated contact between the pesticide molecule and the heteroatom-rich nanoprobe surface. The sensing platform showed concentration-dependent fluorescence quenching over 0.06-50 μg L⁻¹, with a detection limit of 0.018 μg L⁻¹, a quantification limit of 0.060 μg L⁻¹, and 95% signal stabilization within 108 s. Mechanistic analysis indicated dominant static quenching with a partial inner-filter contribution. In forest topsoil extract, runoff water, and plant-surface washings, recoveries ranged from 91.3% to 97.1%, and fluorescence-derived concentrations agreed closely with HPLC-UV validation. These results establish chitosan-derived fluorescent nanoprobes as structurally defined, photophysically stable, and environmentally applicable sensing materials for rapid chlorpyrifos residue monitoring.
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JOURNAL OF HAZARDOUS MATERIALS
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2026
·
IF 10.6
Xinyi Wen,Meiyan Li,Zhongyuan Zhang,Jingwen Wang,Hao Hu,Weihong Wang,Jing Jin,Kairong Wang,Jian Sun,Rui Zhang
Imidacloprid (IMI), a widely used neonicotinoid insecticide, has been frequently detected in drinking water and food; however, whether long-term low-dose exposure to environmentally relevant levels of IMI induces neurotoxic effects in the central nervous system remains unclear. In this study, C57BL/6 N mice were exposed to IMI in drinking water for three、six months at doses derived from human exposure estimates (0, 3 μg/kg, 103 μg/kg, and 6.55 mg/kg). Behavioral tests, including the Morris water maze and novel object recognition, revealed no overt cognitive impairment following IMI exposure. Nevertheless, IMI significantly increased microglial apoptosis in the hippocampus. Mechanistic analyses showed that IMI exposure enhanced JNK phosphorylation and promoted nuclear translocation of FOXO3, accompanied by increased expression of pro-apoptotic proteins BIM and BAX and decreased BCL-2 levels. Consistently, IMI induced apoptosis in BV2 microglial cells through activation of the JNK–FOXO3 signaling pathway, whereas FOXO3 knockdown or JNK inhibition with SP600125 markedly attenuated this effect. These findings indicate that environmentally relevant IMI exposure can trigger hippocampal microglial apoptosis via JNK–FOXO3 signaling even in the absence of overt behavioral deficits, suggesting a potential early neurotoxic effect of neonicotinoid exposure.
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ANALYTICAL CHEMISTRY
·
2025
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IF 6.7
Menghan Liu,Ruyu Zhang,Jie Wang,Huiting Zhang,Jingying Tan,Qingzhi Liu,Lei Han,Lihua Lu
Bimetallic nanozymes not only have strong synergistic catalysis ability but also may improve the catalytic specificity of the nanozymes. Herein, the oxidase (OXD)-mimic Ir/Co bimetallic nanozymes were prepared through a coordination reaction between the Ir(III) complex with the 2-methylimidazole (MeIm) ligands in ZIF-67 under mild conditions without the pyrolysis and physical doping procedures. It is found that the trace amount of Ir-modified ZIF-67 (Ir@ZIF-67) possesses 18.17-fold that of catalytic ability and 7.86-fold that of the reaction rate as those of pristine ZIF-67. On the other hand, compared with ZIF-67, the OXD-like specificity of Ir@ZIF-67 has been increased 9.7%. The theoretical simulation shows that the high-performance of Ir@ZIF-67 comes from its higher O2 adsorption ability. In virtue of the high performing OXD-like catalytic property, Ir/Co bimetallic nanozymes are employed to establish a simple and sensitive colorimetric pesticides detection platform without deleterious H2O2, which offers excellent “switch-on” malathion (MAL) detection in the linear range of 0.05–25 μM with a limit of detection (LOD) of 11.8 nM. Moreover, Ir/Co bimetallic nanozymes are successfully fabricated into the MAL test swabs. Assisted by a color-reading APP, the test swabs can detect MAL with the LOD as low as 18.5 nM. Surprisingly, such a simple test swab can achieve such high sensitivity for MAL detection. The real sample analysis verifies the high potential of the test swabs in practical application. The excellent performance of the fabricated test swabs can promote the formation of the situation where pesticides detection can be tested at any time and any place and can be carried out by anyone. This work not only provides a novel approach to designing polymetallic nanozymes but also displays the promising application of Ir/Co bimetallic nanozymes in the field of colorimetric sensing.
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Nanomaterials
·
2025
·Vol. 15
·期 20
·1596
·
IF 4.3
Yan Yang,Shuojie Wang,Wenxin Mai,Shiyu Wei,Guixiang Teng,Peng Pu,Jiaxing Zhao,Yongqiang Tian
Acetamiprid (ACE) and thiacloprid (THIA) are the dominant cyano-substituted neonicotinoids detected in fruit juices and bottled water, which raises food-safety concerns and regulatory scrutiny. Conventional purification with activated carbon or advanced oxidation shows limited selectivity and has a high energy demand. Covalent organic frameworks (COFs) offer tunable chemistry for targeted adsorption, yet no strategy exists to engineer COF sites that preferentially recognize the cyano group of ACE/THIA. Here, we synthesized a magnetic core-shell adsorbent, Fe3O4@COF(TBTD-BD)-Au, by growing cyano-affinitive Au nanoparticles on a Cl-decorated COF shell surrounding a Fe3O4core. Under optimized conditions (pH 6.0, 25 °C), the Fe3O4@COF(TBTD-BD)-Au achieved maximum adsorption capacities of 157 mg g−1(ACE) and 156 mg g−1(THIA). Uptake followed pseudo-second-order kinetics and the Freundlich isotherm; thermodynamic analysis confirmed an endothermic, spontaneous process. Competitive tests showed >80% removal of ACE and THIA in the presence of four co-occurring neonicotinoids, and the adsorbent retained 91.5% of its initial capacity after six adsorption–desorption cycles. Synergistic Au-cyano coordination, Cl-mediated hydrogen bonding, and π–π stacking confinement confer high selectivity and capacity. This ligand-guided, post-functionalized COF provides promising potential in the field of food sample treatment for contaminant removal.
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Foods
·
2025
·Vol. 14
·期 19
·3423
·
IF 5.1
Chi Zhang,Xin Liu,Tailong Cai,Jianfeng Ping,Yibin Ying
Accurate and rapid extraction of pesticide residues in fruits is crucial for timely food safety monitoring. However, conventional extraction methods remain labor-intensive and time-consuming, often requiring hours for sample preparation. Here, we present a porous hydrogel microneedle (HMN) patch integrated with an automated insertion applicator as a highly efficient platform for the rapid extraction of peach juice for imidacloprid residue detection. The HMN patch, composed of polymethyl vinyl ether/maleic anhydride (PMVE/MA) polymer, was fabricated with high porosity by adjusting the porogen content. Under optimized porogen content of 3% NaHCO 3 , the developed HMN patch exhibited ultrahigh extraction efficiency, achieving a 40-fold water absorption capacity and extracting 0.6% ( w / w ) peach solids of its weight within 5 min. The extracted juice could be readily recovered through a simple elution process and was directly compatible with both high-performance liquid chromatography (HPLC) analysis and lateral flow assays. Compared with conventional destructive methods, the HMN platform offers a scalable, high-efficiency, and user-friendly solution for high-throughput pesticide extraction. The integration of the automated applicator further enhances consistency, minimizes user variability, and facilitates on-site monitoring of pesticide residues, providing a practical pathway for field-deployable food safety monitoring.
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ENVIRONMENTAL RESEARCH
·
2025
·
IF 7.7
Zhongyuan Zhang,Jiaming Fu,Yihong Di,Honghui Li,Bing Wu,Xueyan Tian,Meiyan Li,Yuqing Dai,Zhuoheng Shen,Jinhao Jia,Zeyang Bai,Limeng Xiong,Yuhan Zhang,Xiaoyu Li,Yi Zhao,Hao Hu,GuangJun Wang,Huifang Yang,Rui Zhang,Jian Sun
Previous epidemiologic studies have indicated a positive association between population-based imidacloprid (IMI) exposure and diabetes. Moreover, toxicological evidence suggests that IMI can disrupt glucose metabolism in non-target organisms. However, the toxic doses of IMI in previous experimental studies were much higher than the exposure levels in the real world, and the underlying mechanism remains unknown. In this study, based on the exposure level of neonicotinoids in humans, we investigated the possible mechanism of IMI-induced glucose metabolism disorders using cybertoxicology predictions, a long-term exposure model of IMI in mice, and cellular models. The cybertoxicology predictions showed that NF-κB and apoptosis play key roles in the association of IMI, pancreatic β-cell injury, and diabetes. However, exposure of mice to IMI through potable water (104 μg/kg, population exposure maximum) for 24 weeks did not result in abnormal glucose metabolism (P > 0.05), while significant activation of inflammatory response, NF-κB phosphorylation, and mitochondrial apoptosis pathways were observed (P < 0.05). After inhibition of NF-κB P65 phosphorylation in the cellular model, IMI-induced alterations in the MIN6 cell structure and function, inflammatory responses, and mitochondrial apoptotic pathways were alleviated (P < 0.05). This finding provides evidence for the disruption of glucose metabolism due to long-term exposure to IMI, and subsequent studies should focus on the effects of longer exposure to IMI on glucose metabolism as well as the role of the NF-κB signaling pathway.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2025
·
IF 3.8
Yang Xiaoying,Liu Qian,Luo Longhui,Tian Wei,Kang Chao,Yang Wanliang,Li Tianxiang,Chen Dongmei,Yan Xiufang
A novel biosensing strategy based on the synergy of aptamer molecular recognition and surface-enhanced Raman spectroscopy (SERS) has been developed to address the growing problem of groundwater and soil contamination by the organophosphorus pesticide diazinon (DZN). By combining a high-affinity aptamer with a plasmonic resonance-enhanced substrate, a SERS biosensing interface with single-molecule detection capability was successfully constructed, and the electrochemical surface-enhanced Raman spectroscopy (EC-SERS) method using AgNPs-modified screen-printed electrodes (SPEs) was also used for sensitive detection of DZN. The results showed that the constructed SERS aptamer sensor was able to specifically identify diazinon in the system where multiple interfering pesticides coexisted, and the detection limit of the sensor for diazinon was up to 5.33 × 10 −10 M. The intensity of the characteristic peaks (602 cm −1 ) showed a good linear response with the concentration of DZN, which demonstrated very high detection sensitivity and specific identification function. The proposed EC-SERS method significantly improves the SERS signals of pesticides by potentiometrically modulating the plasmonic resonance coupling effect at the interface of AgNPs-modified electrodes, inducing an orientationally regularised arrangement of DZN molecules. The SERS aptasensor was used to detect diazinon in wastewater and subsurface soil with good recoveries (83.20%–117.78%), which were in good agreement with the results obtained by high-performance liquid chromatography. The results demonstrated that the SERS aptasensor has good sensitivity, stability, simplicity of operation, and specific identification, which provides a solution with ultra-sensitive response, rapid analysis, and strong anti-interference for the detection of trace pesticide.
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