Biomed Research International
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2017
·Vol. 2017
·期 1
·2564363
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IF 2.476
Zuoyi Yang,Junhui Zhou,Yanbin Xu,Yaping Zhang,Haien Luo,KenLin Chang,Yujie Wang
Indole and its derivatives are typical nitrogen heterocyclic compounds and have been of immense concern since they are known for the risk of their toxic, recalcitrant, and carcinogenic properties for human and ecological environment. In this study, a Gram-negative bacterial strain of eliminating indole was isolated from a coking wastewater. The strain was confirmed as Acinetobacter pittii L1 based on the physiological and biochemical characterization and 16S ribosomal DNA (rDNA) gene sequence homology. 400 mg/L indole could be completely removed within 48 h by the strain on the optimum condition of 37°C, pH 7.4, and 150 rpm. The organic nitrogen was converted to NH 3 -N and then to and the organic carbon was partially transferred to CO 2 during the indole biodegradation. The metabolic pathways were proposed to explain the indole degradation based on the liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis of indigo, 4-(3-Hydroxy-1H-pyrrol-2-yl)-2-oxo-but-3-enoic acid, and isatin. The toxicity of the biodegradation products was evaluated using the Microtox test, which revealed that the metabolites were more toxic than indole. Our research holds promise for the potential application of Acinetobacter pittii L1 for NHCs degradation, production of indigoids, and soil remediation as well as treatment of indole containing wastewater.
研究领域:
环境微生物学
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生物降解
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分析化学
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环境毒理学
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废水生物处理
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分子生物学
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Nanoscale
·
2018
·Vol. 11
·期 3
·962-967
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IF 7.233
Zhaohong Su,Xiaolin Xu,Yongbing Cheng,Yueming Tan,Langtao Xiao,Daili Tang,Hongmei Jiang,Xiaoli Qin,Huixian Wang
A porous graphene (PG) bionanocomposite of PG, gold nanoparticles (AuNPs) and anti-indole-3-acetic acid (anti-IAA) antibody for sensitive and label-free amperometric immunoassay of IAA was reported. A PG film was produced by a pre-reduction/electrochemical reduction process on a glassy carbon electrode (GCE) and then a homogeneous AuNPs layer electrodeposition on the PG film. The anti-IAA antibody was immobilized onto the AuNPs through electrostatic adsorption and covalent conjugation. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), Raman spectroscopy, energy dispersive spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV), elecro-chemical impedance spectroscopy (EIS), ultraviolet visible spectroscopy (UV-vis) and differential pulse voltammetry (DPV) were used to characterize the PG film and the stepwise modification of the immunosensor. The electrochemical immunosensor exhibited a wide linear range from 2 × 10−11 to 2 × 10−8 g mL−1 with a detection limit of 0.016 ng mL−1 (S/N = 3) and showed significant linearity R2 = 0.9970. In addition, the proposed immunosensor showed acceptable selectivity and has been applied to the determination of IAA in the extract samples of several plant seeds with acceptable relative derivation (%) ranging from −5.25% to 4.24% between the immunosensor and high performance liquid chromatography. The proposed chemical pre-reduction and electro-reduction guided protocol can be extended to the preparation of many other functionalized PG nanocomposite films for wide applications.
研究领域:
生物传感
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纳米生物技术
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分析化学
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植物生理学
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电化学
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免疫分析
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APPLIED ORGANOMETALLIC CHEMISTRY
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2013
·Vol. 27
·期 3
·148-154
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IF 2.011
Li Qiang Wu
Nano n -propylsulfonated γ-Al 2 O 3 is easily prepared by the reaction of nano γ-Al 2 O 3 with 1,3-propanesultone. This reagent can be used as an efficient catalyst for the synthesis of spiro [indoline-3,4-pyrazolo[3,4- e ][1,4]thiazepine]diones in aqueous media. This new method consistently has the advantages of excellent yields and short reaction times. Further, the catalyst can be reused and recovered several times. Copyright © 2013 John Wiley & Sons, Ltd.
研究领域:
催化化学
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有机合成
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绿色化学
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纳米材料
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药物化学
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POLYMER ENGINEERING AND SCIENCE
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2026
·
IF 3.6
Licheng Wang,Ming Xie,Mingyuan Li,Zhengguo Cai,Junfen Sun,Long Chen
To enhance the thermal-oxidative stability of polypropylene (PP) fiber, several nitrogen-heterocyclic polypropylene copolymers were synthesized as blending modifiers, containing pyrrole (Py), indole (Id), and carbazole (Cz) groups. The large steric hindrance of indole and carbazole groups reduces the negative effect of heteroatoms on the catalytic center, with the highest polymerization activity of 2.33 × 10 4 kg polymer mol Zr −1 h −1 . Compared with PP, the 5% mass loss temperature ( T 5% ) of PPPy and PPId is increased, while only PPPy is capable of further delaying oxygen permeation. The steady-state shear curves indicate that the cross-linked structure between PPPy molecular chains inhibits the flow of PP/NHPP melt, while the indole and carbazole groups promote the flow of PP/NHPP melt. All PP/NHPP blends are successfully prepared into fibers. The mechanical properties of PP/PPPy fiber are well maintained due to the cross-linked structure. The thermo-oxidative stability of PP/NHPP fibers can be enhanced at a low blend content of 5 wt%, with the maximum rise of 13°C in T 5% and 10.7 min in oxidation induction time, which is expected to be applied in engineering fields requiring high-temperature stability. Highlights High polymerization activity of NHPP. NHPP was first incorporated for the melt-spun blend fiber. NHPP enhances the thermo-oxidative stability of blend fiber.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Zhiying Liu,Jinli Ge,Fei Li,Yan Guo,Jianfeng Wang,Xuming Deng,Liuqin He,Hongtao Liu,Shizhen Ma,Jiazhang Qiu
Clostridioides difficile (C. difficile) is a leading cause of antibiotic-associated diarrhea and colitis, with hypervirulent or resistant strains and high recurrence, limiting current therapies. Antivirulence therapy presents a promising alternative by neutralizing toxins without damaging the gut microbiota or promoting resistance. Here, we identified myricetin (MYR) and its derivatives, dihydromyricetin, and myricitrin (MYT), which effectively suppressed TcdB-mediated cell rounding. Mechanistically, MYR inhibits TcdB internalization, self-cleavage, and Rac1 glycosylation, thereby reducing the cytotoxicity. Microbiomics demonstrated that MYR promoted the expansion of beneficial bacteria such as Muribaculaceae and Lactobacillaceae while reducing the abundance of harmful bacteria, including Enterococcaceae and Enterobacteriaceae. Metabolomics further revealed that MYR elevated the levels of indole and its derivatives, including castanospermine. Castanospermine supplementation decreased the fecal bacterial load and ameliorated histopathological damage in infected mice. Collectively, MYR exerts dual functions by antagonizing virulence and sustaining microbiota homeostasis, thereby improving outcomes in C. difficile infections.
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JOURNAL OF HAZARDOUS MATERIALS
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2026
·
IF 10.6
Dongxue Lu,Boyang Chen,Erqi Nie,Shujuan Lian,Rui Li,Rongbo Guo,Shanfei Fu
The emerging understanding highlights indole as a disruptive factor to quorum sensing (QS) mechanisms, prompting further investigation into its role in anaerobic digestion (AD) system inhibition. However, relevant studies are still scarce and the potential mechanism linking indole and AD inhibition remains unclear. This study showed that indole (1, 2, and 3 mM) significantly reduced cumulative methane production by 8.47–51.89% and extended the lag phase by 1.34–6.68 days. Time-series AHLs quantification, metagenomics, and circular clustering heatmaps analysis revealed that indole might disrupt microbial communication between hydrolysis-acidification bacteria and acetoclastic methanogens by reducing the AHLs level (C6-HSL, 3-oxo-C8-HSL, C10-HSL, C12-HSL, 3-oxo-C10-HSL, and C18-HSL). Notably, indole degradation alleviated the inhibition of C10-HSL, C18-HSL, and 3-oxo-C10-HSL, which might restore hydrolysis and acidification and mitigate AD inhibition. Exogenous AHLs (1 and 5 µM) restored methane production by 48.44–55.59% in 3 mM indole-inhibited reactors ( p < 0.05), while the quorum quenching agent vanillin further reduced methane production by 72.55%, suggesting that AHLs play an important role in helping microorganisms resist indole stress. These findings highlight the importance of AHLs-mediated inter-microbial communication in counteracting indole-inhibited methanogenesis inhibition, suggesting potential practical strategies to enhance AD stability and efficiency in challenging conditions.
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INTERNATIONAL JOURNAL OF FOOD PROPERTIES
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2026
·
IF 3.9
Nian Cao,Yubo Yang,Xiaoling Xiong,Bohan Zhang,Ping Xiang,Fan Yang
This pioneering study investigated the effects of sip volume on sensory perception and drinking comfort during the consumption of Jiangxiangxing Baijiu (JB). Six sip volumes (6, 9, 12, 15, 18, and 20 mL) were tested with 30 participants (15 males and 15 females). Results revealed gender-specific preferences: females tended to prefer sip volumes of 9–12 mL, while males were more likely to favor 18 mL. Using Temporal Dominance of Sensation (TDS) and Check-All-That-Apply (CATA) methods, it was found that oral sensations of sweetness and sauce flavor, as well as post-pharyngeal sensations of “smoothness,” “mellowness,” and “lingering aftertaste,” enhanced drinking comfort. In contrast, oral sensations of spiciness and bitterness, along with post-pharyngeal sensations of “pungency,” “thinness,” “blandness,” and “momentary aftertaste,” impaired comfort. Notably, females may place greater emphasis on oral perception, whereas males could focus more on post-pharyngeal sensations. A novel flavor analysis identified 20 key compounds that influence JB perception. The insights from this research provide valuable contributions to improving the drinking experience of JB.
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Virulence
·
2026
·
IF 5.4
Yu Zheng,Luhua Fu,Zhuoying Cao,Ting Zhang,Jiao Fei,Ming Jiang,Yuying Zhou,Zhi Shi,Yubin Su
Bacterial metabolism is important for antibiotic resistance and tolerance. However, the impact of indole on bacterial metabolism and antibiotic efficacy has not been fully elucidated. In this study, we investigated the effect and specific mechanism of exogenous indole on the antibiotic susceptibility of Edwardsiella tarda, a common pathogen in freshwater and marine fish farming. We found that exogenous indole promoted E. tarda tolerance to the antibiotic florfenicol, and reprogrammed the E. tarda metabolome. A total of 108 metabolites were detected, including 66 differential metabolites that regulate various metabolic pathways, such as the tricarboxylic acid (TCA) cycle and nucleotide metabolism. Exogenous indole disrupted the TCA cycle in E. tarda by increasing the intracellular NADH contents and activating the respiratory chain to increase the reactive oxygen species levels, thereby increasing the intracellular Fe2+ content to activate the Fenton reaction, which in turn promotes the oxidative stress response. Furthermore, indole inhibited antibiotic entry into the cell and activated efflux pumps to reduce the intracellular antibiotic content, ultimately promoting antibiotic tolerance. In vivo, exogenous indole compromised the ability of florfenicol to protect fish survival and eliminate pathogenic bacteria. These results shed light on the metabolic changes induced by indole and suggest future directions for addressing antibiotic tolerance and clinical infections of E. tarda in aquaculture. This study serves as a reminder of the adverse effects of combining antibiotics with metabolites in aquaculture.
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CARBOHYDRATE POLYMERS
·
2026
·
IF 13.2
Yuqing Sun,Shi Zhong,Jinxi Huo,Qin Xiao,Huanyan Ma,Xintian Dong,Jiayi Feng,Meiliang Pan,Zhengshun Wen,Yougui Li
Mulberry leaf is a traditional Chinese herbal medicine with hepatoprotective properties, but the role of its polysaccharides in counteracting drug-induced liver injury (DILI) remains unexplored. Herein, a polysaccharide named MBPS-02A was isolated from mulberry leaves, which featured a backbone of 1,2-α-Rha p , 1,4-α-Gal p A, 1,4-α-Gal p and 1,6-β-Gal p , with branches including 1,4-β-Gal p , t-β-Gal p , t-β-Glc p A, 1,5-α-Ara f , t-α-Ara f and t-α-Gal p A. MBPS-02A attenuated acetaminophen (APAP)-induced DILI in mice, as evidenced by reduced hepatic inflammatory damage and oxidative stress. Additionally, it strengthened intestinal barrier integrity and reshaped the gut microbiota, promoting Akkermansia proliferation both in vivo and in vitro. Correlation analysis identified the Akkermansia -derived tripeptide glycylprolylarginine (GPR) as a key metabolite strongly associated with DILI amelioration. Cellular experiments confirmed that GPR scavenges hydrogen peroxide and upregulates catalase expression in hepatocytes, thereby protecting liver cells. Collectively, these results suggest MBPS-02A as a promising natural polysaccharide candidate for DILI intervention via the gut-liver axis, and provide mechanistic insights into the hepatoprotective use of mulberry leaves.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Jing Wang,Ruifang Wang,Junyi Wang,Yan Gao,Lina Qiao,Ning Zhang
Qingke(highland barley) is a unique Tibetan Plateau crop, vital for local diets and traditional products. However, flavor differences among its varieties remain unclear. This work examined 4 representative Qingke varieties to investigate their flavor profiles and key aroma contributors. 60 aroma compounds were identified in the 4 Qingke varieties using gas chromatography–mass spectrometry and aroma extract dilution analysis. The results revealed that black/white and blue/yellow Qingke had similar flavor profiles respectively. Besides, black and white Qingke possessed more intense and abundant aromas, with 81 and 77 compounds, respectively. These two varieties were selected for further flavor analysis by aroma recombination experiment, which identified ( E )-2-octenal, vanillin, γ -caprolactone, 3-methylthiopropanal and 4-vinylguaiacol as key odorants. Additionally, 1-pentanol, 3-hydroxy-2-butanone, phenylethanol and guaiacol were found to be the flavor differentiators of black and white Qingke using omission experiment. This work provides a scientific foundation for Qingke's flavor quality assessment, product development and future applications.
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PHYTOMEDICINE
·
2026
·
IF 8.3
Qi Wu,Ziyi Chen,Zong Hou,Zhiqiang Liu,Rong Sun,Shu Liu
Background Polygoni Multiflori Radix (PMR) and its processed form, Polygoni Multiflori Radix Praeparata (PMRP), are two widely used traditional Chinese medicines (TCM). However, in recent years, frequent reports have emerged regarding their hepatotoxicity. Despite numerous studies, the underlying mechanisms of hepatotoxicity and key toxic components remain poorly understood. Purpose This study aimed to comprehensively elucidate the hepatotoxic processes of PMR and PMRP and identify the principal toxic components. Methods In vivo toxicity tests were carried out to assess the toxicity levels and characteristics of PMR and PMRP. The integration of untargeted serum metabolomics, liver spatial transcriptomics, and liver spatial metabolomics was first employed to elucidate the toxicity mechanisms, which were further validated through metabolite and sensitive index levels and by evaluating protein expression. Mass spectrometry and cytotoxicity tests were utilised to determine the primary toxic components. Results The findings revealed that PMR and PMRP primarily regulate tryptophan metabolism, the tricarboxylic acid (TCA) cycle, purine metabolism, and glutathione metabolism. Furthermore, PMR and PMRP can inhibit the expression of bile acid transporters, causing obstruction of bile acid secretion. These modulations trigger oxidative stress, which subsequently leads to cholestasis. The accumulation of bile acids further intensifies oxidative stress, creating a vicious cycle. Furthermore, emodin was identified as the primary toxic component. Conclusion PMR and PMRP can induce cholestatic liver injury. They exert hepatotoxic effects by establishing a vicious cycle between cholestasis and oxidative stress, with emodin being the key component responsible for this toxicity.
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FOOD CHEMISTRY
·
2026
·
IF 9.8
Yan Zhao,Chen-Yang Shao,Han Yan,Yue Zhang,Qun-Hua Peng,Zhi Lin,Xiao-Ting Zhai,Hai-Peng Lv,Yin Zhu
Tea cultivars significantly influence aroma, yet essential odorants linked to specific cultivars remain underexplored. In this study, an integrated sensomics approach identified 57, 50, and 59 odorants in three representative baked green tea cultivars using sequential stir-bar sorptive extraction coupled with gas chromatography–mass spectrometry/olfactometry, ultimately pinpointing eight key cultivar-specific odorants. The “sweet/green” notes in ‘Zhongcha 108’ were primarily attributed to cedrol (18.52 ng/mL, enhancing aroma by up to 176 % compared to the blank matrix). Indole, methyl salicylate, geraniol, and γ -dodecalactone were closely associated with the “floral” character of ‘Zhongcha 302’ (1.30–895.86 ng/mL, increasing aroma by up to 510 %). Viridiflorol, theaspirane B, and α -ionone contributed to the “sweet/floral” notes in ‘Fuding Dabaicha’ (trace levels to 12.47 ng/mL, enhancing aroma by up to 163 %). Furthermore, the aroma profile of ‘Zhongcha 302’ was successfully reconstructed based on its quantified odorants, achieving >90 % similarity to the original tea.
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