FOOD MICROBIOLOGY
·
2026
·
IF 5.4
Liangliang Gao,Tengjie Li,Zengrong Xiong,Mingnan You,Jun Zhang,Nengguo Tao
In this study, N'-(4-fluorophenyl)-2-pyridinecarbohydrazide (2-FPH), N'-(4-fluorophenyl)-3-pyridinecarbohydrazide (3-FPH), and N'-(4-fluorophenyl)-4-pyridinecarbohydrazide (4-FPH), which showed considerable antifungal activities against the prochloraz-resistant Penicillium italicum strain (PiR2), were designed and synthesized. Among these, 2-FPH displayed the strongest antifungal activity against PiR2 growth, with minimum inhibitory concentrations (MIC) of 12.5 μg/mL and minimum fungicidal concentrations (MFC) of 25 μg/mL. In vivo antifungal and hemolytic toxicity tests showed that 2-FPH effectively prevented PiR2 infection of citrus fruits, but exhibited exceptionally low hemolytic toxicity. At equivalent concentrations, its biological safety and control efficacy were far superior to those of prochloraz. According to morphological findings, 2-FPH increased cell membrane permeability, caused cell swelling and morphological abnormalities, disrupted structural integrity, and decreased chitin content in cell walls. Physiological, biochemical, and molecular biology investigations indicated that 2-FPH binds to succinate dehydrogenase (SDH), inhibits its enzymatic activity, and suppresses associated gene expression. This decreases intracellular ATP levels, disrupts normal mitochondrial function, and triggers a substantial increase in reactive oxygen species (ROS). In summary, 2-FPH shows promising potential as a possible fungicide and succinate dehydrogenase inhibitor for managing citrus blue mold.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2026
·
IF 7.5
Junwei Yao,Qian Wang,Chunxin Wang,Hong Qi,Qingshan Shi,Jingxia Liu,Jin Feng,Ping Yang,Manli Yu,Xiaobao Xie
Attributing to poor effectiveness and inadmissible toxicity, fungicide is a tendency to restricted in the field of agriculture. Therefore, it was a prospective scene to exploit the inventive antifungal active ingredient delivery system for improving biological activity and environmental safety. The excellent adhesive fungicide formulation was endowed with an effective tactic to promote wettability and bioavailability. The elemental intention of this study was to illuminate synergistic mechanisms of dopamine (DA) coated prochloraz (Pro)-polycaprolactone (PCL) nanospheres on prominent roles of improving antifungal activity and environmental safety. Apart from enhancing wettability and anti-photodegradation ability, Pro-PCL-DA nanospheres was also able to reduce the burst release rate and prolong release duration for the phenolic hydroxyl group in DA. The Pro-PCL-DA nanospheres was charged with the superior antifungal activity against Fusarium oxysporum . The predominant antifungal activity of Pro-PCL-DA nanospheres was predominantly ascribed to superior transmembrane transport capacity, inducing excessive reactive oxygen species and enhancing membrane permeability. The environmental safety assay indicated that Pro-PCL-DA nanospheres could effectively reduce the acute toxicity of Pro to adult zebrafish and Eisenia fetida . Overall, these findings indicated that DA coated Pro-PCL nanospheres was potential route to provide a bright platform for improving fungicide utilization with the long-term sustainable development of agriculture.
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2025
·
IF 2.5
Qianyu Cai,Yucheng Chen,Ting Wang,Qiongmei Mai,Jianglong Hu,Yunyou Lv,Yanhui Zhou,Jie Liu
The advancement of stimulus-responsive pesticide delivery systems offers a pivotal strategy for reconciling agricultural productivity with ecological sustainability. This study engineers a cellulose-functionalized vaterite calcium carbonate (CaCO3) nanoplatform (Pro@CaCO3@HPC, PCH) endowed with pH/cellulase dual-responsive intelligence for targeted prochloraz delivery. The hierarchically porous vaterite architecture facilitates superior drug loading (12.4%, w/w), while demonstrating environmentally triggered release kinetics. Controlled liberation studies reveal 2.37-fold and 2.13-fold cumulative release enhancements under acidic conditions and cellulase exposure, respectively, compared to neutral environments—a response mechanism strategically aligned with the pathophysiological microenvironment of Sclerotinia sclerotiorum infection. The multifunctional nanoformulation exhibits threefold advantages: (1) enhanced antifungal performance, achieving 96.1% mycelial inhibition at 1 µg mL−1 compared to conventional formulations (72.7%); (2) reduced environmental footprint through 27.7% decreased soil mobility relative to free prochloraz; (3) improved biosafety profiles, maintaining crop germination rates 13.3% higher than commercial formulations while demonstrating attenuated cytotoxicity. This phytopathology-activated delivery system establishes a technological paradigm that synchronizes three critical agricultural requirements: pathogen-responsive precision, environmental persistence mitigation, and non-target organism protection. Therefore, this study provides an environmentally friendly, intelligent and efficient strategy, which shows great potential in the field of Sclerotinia sclerotiorum control.
✓ 已验证
产品关联
DOI
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2026
·
IF 6.8
Xiumei Chen,Chen Zhu,Junyu Chen,Chaobo Bing,Qiuli Ouyang,Xiangrong Zhu,Nengguo Tao
The citrus green mold caused by Penicillium digitatum is a wound-mediated disease considered the main cause of post-harvest citrus fruit spoilage. In this study, an antifungal composite aerogel based on carboxymethyl cellulose (CMC) and polyvinyl alcohol (PVA) was prepared and used to controlling green mold in citrus fruit with a combination of cinnamaldehyde and citronellal (CC). Characterization revealed that the CMC/PVA aerogel had a three-dimensional porous structure, and hydrogen-bond interactions existed between -C O groups of CMC and -OH groups of PVA. The composite aerogels had lower porosity, higher density and lower hygroscopicity performance than raw CMC aerogels. When loaded with CC (4:1, v/v), the aerogel enabled continuous release to fifteen days, with its maximal release rate on the second day. The release profiles of the CMC/PVA-CC were fitted well by the Korsmeyer–Peppas model and the release behavior was governed by Fickian diffusion. In vitro and in vivo tests showed that these aerogels inhibited P. digitatum growth in a concentration-dependent manner. Moreover, CMC/PVA composite aerogels loaded with 0.01 μL L −1 CC suppressed postharvest green mold decay in citrus fruit, demonstrating efficacy comparable to that of the 0.025 % prochloraz, while maintaining fruit quality after storage of 30 d. These results indicated that CC-loaded CMC/PVA composite aerogels can serve as a sustained release and eco-friendly alternative to conventional chemical fungicides for managing postharvest diseases in citrus fruit.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Honglin Qin,Yuan Tao,Ruiting Niu,Bingyan Han,Lizhen Qiao
In this work, magnetic flower-like and fluorine-doped nickel oxide composites Fe 3 O 4 @SiO 2 @F/NiO were prepared and applied for the magnetic solid-phase extraction (MSPE) of five imidazole fungicides. The obtained materials were characterized by scanning electron microscopy, X-ray diffraction, energy-dispersive X-ray photoelectron spectroscopy and Brunauer-Emmett-Teller analysis to verify the successful synthesis. The effect of some experimental parameters on the extraction efficiency were explored to optimize the MSPE conditions, including the adsorbent dosage, ultrasonic time, salt concentration, sample solution pH, elution solvent type and volume. Then, method validation was performed under the optimal conditions in combination with high-performance liquid chromatography-ultraviolet detection. The determination coefficients ranged from 0.9956 to 0.9991, and the limits of detection and limits of quantification for the imidazole fungicides were 0.1–3 μg·L −1 and 0.3–10 μg·L −1 , respectively. Moreover, the intra-day and inter-day precisions were evaluated in three different concentrations with relative standard deviations in the range of 1.34 %-13.31 % and 4.96 %-13.92 %. At last, the proposed MSPE method was used to enrich and determine imidazole fungicides in tap water, milk and green tea samples, and the spiked recoveries were in the range of 77.93 %-119.38 %, demonstrating the potential of the method in the determination of imidazole fungicides from real samples.
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Wanqi Jiang,Zan Yang,Fei Tong,Siyu Zhang,Lu Zhu,Lei Wang,Lunjing Huang,Kang Liu,Mingming Zheng,Yibin Zhou,Ruyan Hou,Yingnan Liu
In this study, based on the oxidase activity and photothermal effect of manganese dioxide nanosheets (MnO 2 NSs), with thiamine (TH) as the fluorescence response signal and tris (2,2'-bipyridyl) ruthenium (II) hexahydrate as the reference signal, an enzyme-regulated ratiometric fluorescence and photothermal dual-mode probe was constructed for the quantitative detection of organophosphorus pesticide (OPs) residues. OPs reduced the production of the reductive product thiocholine by inhibiting the activity of acetylcholinesterase , thereby regulating the residual amount of MnO 2 NSs. With the increase of OPs concentration, the color of the probe solution gradually transitioned from red to blue, and the temperature gradually increased. Using dichlorvos and chlorpyrifos as pesticide models, the developed probes exhibited sensitive responses to OPs in a wide linear range of 0.1–8000 ng/mL. The detection limits of dichlorvos and chlorpyrifos in fluorescence mode were 1.13 × 10 −3 ng/mL and 0.86 ng/mL, respectively. The corresponding detection limits in photothermal mode were 1.01 ng/mL and 1.02 ng/mL, respectively. The proposed probe displayed excellent anti-interference and reliability in the analysis of OPs residues in real samples. The dual-mode probe with self-verification function is expected to provide more accurate and robust detection results than the single-mode probe, and has a wider application prospect.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Siting Lai,Li Li,Qian Li,Shijiang Zhu,Guang Wang
Internal browning (IB) is a physiological disorder without external symptoms of postharvest pineapples, but whether and how IB influences pineapple volatiles remain unknown. We examined eigenvalues of volatiles in ‘Comte de Paris’ pineapples with or without IB using electronic nose (E-nose) and gas chromatography-mass spectrometry (GC–MS). Correlation coefficients between the responses of E-nose sensors S7 and S9 and IB were 0.836 and 0.804, respectively. The multilayer perceptron neural network and radial basis function neural network models classified IB degree with accuracy of 94.77% and 91.67%. GC–MS analysis revealed 30 volatile substances upregulated in pineapple with IB compared to those without, of which 15 were esters. IB regulated volatile compound synthesis through the lipoxygenase pathway which involved lipoxygenase , pyruvate decarboxylase 1, alcohol dehydrogenases , acyl-CoA oxidase 1 , and alcohol acyltransferase genes and their related enzymes. These results suggested that volatiles regulated by IB could be used to discriminate IB severity in pineapples.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2023
·
IF 7
Yuwei Luo,Xin Chen,Jin Xin Che,Yonghua Zhang,Qiuli Ouyang,Nengguo Tao
Blue mold caused by Penicillium italicum is one of the most devastating postharvest diseases in Citrus fruit. This work aims to determine the antifungal potential of ( E )-2-octenal, a component of plant essential oil, on the mycelial growth and disease incidence of a prochloraz-resistant strain of P. italicum isolated from an orchard in Luxi, Hunan Province, China. An in vitro assay revealed that the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of ( E )-2-octenal were 0.25 and 0.50 mL L −1 , respectively. An in vivo assay showed that wax + 4 × MFC ( E )-2-octenal treatment could reduce the blue mold incidence in Ponkan fruit inoculated with P. italicum without impairing fruit quality. Scanning electron microscopy analysis illustrated that the mycelia of P. italicum were anomalously twisted and shrunken upon treatment with ( E )-2-octenal. The primary antifungal targets were attributed to the cell membrane instead of the cell wall based on propidium iodide and calcofluor white staining results. In addition, ( E )-2-octenal induced massive accumulation of reactive oxygen species in hyphae in a dose-dependent manner, leading to oxidative stress and dysfunction of energy and respiration metabolism. At the same time, the activities of phosphofructokinase (PFK) and pyruvate kinase (PK) were decreased in ( E )-2-octenal-treated samples. Taken together, our present study suggested that ( E )-2-octenal could exert its antifungal activity against P. italicum by damaging the function of mitochondria and may be a potential antifungal substance for an alternative to chemical fungicides.
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Junwei Yao,Heng Zhi,Qingshan Shi,Yu Zhang,Jin Feng,Jingxia Liu,Hui Huang,Xiaobao Xie
With the poorly soluble and intrinsically unstable feature, prochloraz (Pro) was confronted with lower bioavailability in the crop defense against fungal erosion. Therefore, it was a challenging project to explore the innovative antifungal compound delivery system for improving bioavailability. The superior adhesive fungicide formulation was supposed to be an efficient pathway to enhance transmembrane permeability and biological activity. According to abundant phenolic hydroxyl groups, tannic acid (TA) was an ideal modified adhesive biomaterial to improve interfacial interactions. The fundamental purpose of this research was focused on the synergistic mechanism of TA-interfacial-modified Pro-ethyl cellulose (EC) nanoparticles for improving bioavailability and biosafety. In the stability test, TA-modified Pro-EC nanoparticles had the capacity to reduce Pro initial release burst, extending a persistent validity and improving anti-photodegradation property. The toxicity index of Pro-EC and Pro-EC-TA was approximately 2.93-fold and 4.96-fold that of Pro technical against Fusarium graminearum (F. graminearum), respectively. Compared with nonmodified EC nanoparticles, TA-modified EC nanoparticles obtained eminent transmembrane permeability and superior adherence ability to F. graminearum, for hydroxyl and carboxyl groups of TA to enhance interaction with target cell membranes. The contents of cellular reactive oxygen species induced by Pro-EC and Pro-EC-TA nanoparticles were about 2.31 times and 3.00 times that of the control check (CK), respectively. Compared to the CK group, the membrane potential and ergosterol values of F. graminearum treated with Pro-EC-TA nanoparticles were drastically reduced by 74.91 and 56.20%, respectively. In the biosafety assay, the maximum half-lethal concentration value of the TA-modified Pro-EC nanoparticles indicated that the acute toxicity of the Pro-EC-TA nanoparticles to adult zebrafish was approximately 8.34-fold reduced compared to that of the Pro technical. These findings demonstrated that the successful interfacial modification of Pro-EC nanoparticles with TA was a highly efficient, environmentally safe, and promising alternative for sustainable agricultural application, thus making the fungicide formulation process more simplified, easier fabrication, and lower cost.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Toxics
·
2022
·Vol. 10
·期 8
·435
·
IF 4.472
Jiajian Huang,Sukun Lin,Jingtong Zhou,Huiya Chen,Shiqi Tang,Jian Wu,Suqing Huang,Dongmei Cheng,Zhixiang Zhang
Background: As a systematic fungicide, prochloraz is often used to control banana freckle disease, and it is significant to assess the safety and risk of prochloraz. Methods: The dissipation kinetics and distribution of prochloraz in bananas were measured by high-performance liquid chromatography (HPLC). Results: The results showed that the fortified recoveries in bananas were 83.01–99.12%, and the relative standard deviations (RSDs) were 2.45–7.84%. The half-life of prochloraz in banana peel (3.93–5.60 d) was significantly lower than it was in whole banana (8.25–10.80 d) and banana pulp (10.35–12.84 d). The terminal residue of prochloraz in banana fruits was below the maximum residue level (MRL, China) at pre-harvest intervals (PHI) of 21 d. Moreover, the residue of prochloraz in banana peel was always 1.06–7.71 times greater than it was in banana pulp. The dietary risk assessment results indicated that the prochloraz residue in bananas at PHI of 21 d was safe for representative populations. (4) Conclusions: We found that a 26.7% prochloraz emulsion oil in water (EW) diluted 1000-fold and sprayed three times under field conditions was safe and reliable, providing a reference for the safe application of prochloraz in bananas.Keywords:banana;prochloraz;high-performance liquid chromatography;dissipation dynamics;distribution;dietary risk assessment
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Fungi
·
2022
·Vol. 8
·期 5
·547
·
IF 5.724
Gui-Yang Zhu,Ying Chen,Su-Yan Wang,Xin-Chi Shi,Daniela D. Herrera-Balandrano,Victor Polo,Pedro Laborda
Fungal pathogens can invade not only the fruit peel but also the outer part of the fruit mesocarp, limiting the efficacy of fungicides. In this study, the relationships between fungicide structure, diffusion capacity and in vivo efficacy were evaluated for the first time. The diffusion capacity from pear peel to mesocarp of 11 antifungal compounds, includingp-aminobenzoic acid, carbendazim, difenoconazole, dipicolinic acid, flusilazole, gentamicin, kojic acid, prochloraz, quinolinic acid, thiophanate methyl and thiram was screened. The obtained results indicated that size and especially polarity were negatively correlated with the diffusion capacity. Although some antifungal compounds, such as prochloraz and carbendazim, were completely degraded after a few days in peel and mesocarp, other compounds, such asp-aminobenzoic acid and kojic acid, showed high stability. When applying the antifungal compounds at the EC50concentrations, it was observed that the compounds with high diffusion capacity showed higher in vivo antifungal activity againstAlternaria alternatathan compounds with low diffusion capacity. In contrast, there was no relationship between stability and in vivo efficacy. Collectively, the obtained results indicated that the diffusion capacity plays an important role in the efficacy of fungicides for the control of pear fruit diseases.Keywords:fungicides;plant fungal pathogens;pear fruit;antifungal activity;peel diffusion;postharvest diseases
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHYTOPATHOLOGY
·
2022
·Vol. 170
·期 6
·373-381
·
IF 1.826
Ruofan Wei,Rong Wang,Yong Li,Moyi Yue,Wanlong Ding
Zhi mu ( Anemarrhena asphodeloides Bunge.) is an important medicinal plant in China, and its growth is greatly threatened by the fungal disease anthracnose, which can be caused by a number of species of Colletotrichum . Studying the phytopathogen and its sensitivities to fungicides will help to control anthracnose on zhi mu. Ten isolates of Colletotrichum were collected from zhi mu in Hebei, Anhui and Shanxi Provinces in China for this study. The isolates were identified as C . gloeosporioides , C. liriopes and C. spaethianum based on multi-locus phylogenetic analyses, including ACT , CHS - 1 , GADPH , HIS3 and ITS , micromorphological characteristics and the characteristics of colonies. Koch's postulates were confirmed by inoculating the leaves of zhi mu in vitro . The levels of virulence of 10 isolates varied significantly. Isolate YC-1 was the most virulent, while BZ-2 was the least virulent. The sensitivity of isolates YC-1, BY-1 and BY-2 ( C . spaethianum , C. gloeosporioides and C. liriopes respectively) to fungicides were examined. The EC 50 values of BY-1, BY-2, and YC-1 to tebuconazole, difenoconazole, prochloraz, fludioxonil, pyraclostrobin, carbendazim and azoxystrobin confirm that all of the isolates were markedly sensitive to the seven fungicides tested, strongly suggesting that these fungicides could be used to control anthracnose on zhi mu in the field.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）