Advanced Healthcare Materials
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2025
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IF 9.6
Yunfeng Tang,Xiangtian Deng,Min Yi,Dong Wang,Cheng Zheng,Shichang Zhao,Renliang Zhao
Melanoma remains one of the most aggressive skin cancers with high recurrence rates following surgical resection. To overcome the challenges of residual tumor cells and postoperative immune resistance, an injectable hydrogel is developed that co-delivers propranolol (Pro) and imiquimod (IMQ). The rationale is to achieve a synergistic therapeutic effect by enhancing immunogenic cell death (ICD) and strengthening antitumor immunity. The hydrogel is synthesized via boronate ester crosslinking between HA-EGCG and CMCS-PBA and demonstrates excellent injectability, self-healing, biodegradability, and tissue adhesion. In vitro, Pro potentiated IMQ-induced reactive oxygen species (ROS) production, enhancing ICD markers such as calreticulin exposure (CRT), high mobility group box 1 (HMGB1), and adenosine triphosphate (ATP) release, thereby promoting dendritic cell maturation and T cell activation. In vivo, Pro/IMQ@ Hydrogel (PIH) effectively inhibited tumor recurrence in an incomplete melanoma resection model, significantly reducing tumor volume and inducing apoptosis without systemic toxicity. Immunohistochemical analyses revealed increased CD8⁺ and CD4⁺ T cell infiltration, elevated inflammatory cytokine levels, and enhanced memory T cell responses. This work highlights the potential of PIH as a potent localized chemo-immunotherapy platform for melanoma by converting “cold” tumors into “hot” immunogenic lesions.
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
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IF 8.7
Zhichao Song,Shujie Xu,Bo Xu,Man Zhou,Ying Liu,Ying Zhang,Li Cui,Ping Wang,Qiang Wang,Yuanyuan Yu
This study prepared high-strength, colored and multifunctional polyphenol-chitosan fibers via a novel strategy of laccase-mediated phenolic-amine crosslinking within chitosan fibers in a swollen state. A dense covalent network within the fiber was formed through Michael addition and Schiff base reactions. Compared to unmodified chitosan fibers, the enzymatically cross-linked fibers exhibited significantly enhanced mechanical properties, with an increase of about 270% in dry tensile strength (up to 156.8 MPa) and excellent wet stability. In addition, the polyphenol-chitosan fibers were given color and different properties by the covalent cross-linking of polyphenols into the fiber. The polyphenol-chitosan fibers had stable colors and did not fade in the environment. They also had an exceptional antibacterial rate of 99.99% and excellent UV protection. This laccase-mediated phenolic-amine crosslinking strategy provides an effective, sustainable method for producing high-performance, bio-based functional fibers.
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FOOD HYDROCOLLOIDS
·
2026
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IF 13.8
Wei Liang,Xuhui Huang,Marat Muratkhan,Chengrong Wen,Lei Qin
Designing starch-based soft gels with visual functionality and modulated digestibility is important for developing texture-modified foods. In this study, blue wheat starch composite gels were constructed by incorporating phycocyanin (Phy, 5% and 10%) and chitooligosaccharide (COS, 2.5% and 5%), and their formation, multiscale structure, physicochemical properties, and in vitro digestibility were systematically investigated. Phy imparted a blue to blue-green appearance to the gels, while COS further modulated their color characteristics. Phy and COS reduced pasting viscosity and viscosity rebuilding and promoted the transition from a dense, starch-dominated gel network toward a looser and more heterogeneous composite structure. Structural and thermal analyses revealed reduced short-range order, relative crystallinity, and gelatinization enthalpy, accompanied by weakened viscoelasticity and lower textural strength. The incorporation of Phy and COS also redistributed the digestible starch fractions, with increased RDS and decreased SDS and RS observed in several composite formulations. Molecular docking suggested that starch-derived glucan chains and COS could potentially recognize partially overlapping regions on the Phy surface, providing a possible molecular basis for local component association and subsequent structural reorganization. Overall, these findings demonstrate that Phy and COS regulate wheat starch gel properties through formulation-dependent structural reorganization, providing a basis for designing visually distinctive and soft-textured starch-based composite gels with modulated digestion profile.
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高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2026
·Vol. 36
·期 62
·e76841
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IF 19.9
Lijun Lu,Xingwei Chen,Yujie Zhang,Jing Liu,Chang Liu,Yun Hu,Yanchao Mao
Sustainably derived all-natural conductive hydrogels offer a new class of soft materials with intrinsic biocompatibility, biodegradability, and tunable mechanical properties, showing great promise for flexible bioelectronic interfaces. However, their practical application remains limited by inherently low electrical conductivity. Ion channels in the human nervous system, composed of proteins and oligosaccharides, can reduce the effective radius of hydrated ions by inducing partial dehydration of the hydration shell, thereby enabling highly efficient and ordered ion-proton cooperative transport. Inspired by this ability, an all-natural highly conductive ion-proton bipathway hydrogel (IPBH) composed of bovine serum albumin (BSA), chitosan oligosaccharide (COS), and Ca 2 + was developed to mimic natural ion transport environments and significantly enhance the conductivity. The resulting IPBH achieves a remarkable conductivity of 40.59 mS/cm, which is 1272% and 384% higher than that of the proton-only and ion-only conductive hydrogels, respectively. Besides, the IPBH exhibits complete biodegradability within 36 h and exceptional biocompatibility. Moreover, the IPBH exhibits excellent low-noise performance in physiological signal monitoring, enabling accurate EMG-based gesture recognition integrated with a convolutional neural network (CNN) model for real-time robotic arm control. This synergistically conductive, ion-proton coupled hydrogel provides a versatile and sustainable platform for next-generation high-performance bioelectronic interfaces.
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CERAMICS INTERNATIONAL
·
2026
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IF 6
Kui Ding,Anqi Sheng,Jing Song,Shuyan Song,Hongjie Zhang,Lang Zhao
To address the low removal rate, severe surface damage, and unclear mechanism of A-plane sapphire during chemical mechanical polishing, a novel Al 2 O 3 -CeO 2 mixed-abrasive slurry was developed by integrating the mechanical abrasion of Al 2 O 3 with the chemical reactivity of CeO 2 . The optimal formulation, containing 2 wt% Al 2 O 3 , 0.2 wt% CeO 2 , 0.15 wt% sodium hexametaphosphate, 0.7 wt% isooctanol polyoxyethylene ether, and 0.5 wt% sodium D-gluconate with aminomethyl propanol as pH regulator, yields an atomically smooth surface (Ra = 0.079 nm) and a high removal rate (MRR = 905 nm/h). XPS analysis reveals that a hydrated Al-OH layer and a CeAl x O y soft phase form on the sapphire via interfacial solid-state reaction, and are removed by mechanical shearing of the mixed abrasives.
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FOOD RESEARCH INTERNATIONAL
·
2026
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IF 8.8
Wei Wan,Xiaokang Na,Ming Du,Jianyu Zhu,Beiwei Zhu,Chao Wu
7S and 11S globulins exhibit distinct binding modes with chitosan oligosaccharide. • The 7S/11S ratio determines the gelation pathway and network architecture. • 11S-rich systems form rigid, covalently cross-linked hydrogels. • Globulin composition modulates water mobility and mechanical properties. Soy protein isolate (SPI) is widely used in food applications, but the natural variation in its 7S/11S globulin ratio leads to inconsistent gel properties. In this study, we investigated how the 7S/11S ratio influences the structural and rheological properties of composite hydrogels formed with chitosan oligosaccharide (COS). Spectroscopic analyses revealed that 7S-rich systems maintain a more flexible conformation with higher α-helix content (12.7%), whereas 11S-rich systems adopt a rigid, β-sheet-rich structure with β-sheet content increasing to 49.6% (compared to 34.7% in 7S-rich systems). Rheological measurements showed that increasing the 11S fraction progressively enhances the storage modulus, which reaches approximately five times that of 7S-rich gels, transforming the gel network from physically entangled to covalently cross-linked. Microstructural and water mobility analyses confirmed that 11S-rich gels form dense, compact structures that restrict water mobility, with transverse relaxation time decreasing from 238 ms to 180 ms, whereas 7S-rich gels exhibit looser structures. These findings demonstrate that the 7S/11S ratio is a critical determinant of the macroscopic properties of SPI-COS composite gels, providing a practical strategy for tailoring plant protein gel textures. Download: Download high-res image (200KB) Download: Download full-size image
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2026
·
IF 5.9
Liying Qin,Xian Wu,Zirong Li,Li Cui,Yuanyuan Yu,Qiang Wang,Fenglai Yuan,Ping Wang
Phosphorylation and phenolic modification enhanced chitosan oligosaccharide reactivity. • Enzymatic grafting of modified chitosan oligosaccharides stabilized silk fibroin. • Modified silk fibroin scaffolds promoted efficient biomimetic mineralization. • Mineralized scaffolds enhanced mechanics and osteoblast differentiation. Silk fibroin (SF) is widely used in biomedical materials owing to its biocompatibility and biodegradability. However, natural SF scaffolds lack sufficient calcium-binding motifs, particularly carboxylate groups derived from acidic amino acid residues, thereby hindering efficient nucleation and oriented growth of hydroxyapatite (HAp) during biomimetic mineralization, and ultimately compromising the mechanical properties of the resulting scaffold. To address this, we functionalized chitosan oligosaccharide (COS) with phosphate and phenolic hydroxyl groups to yield a highly reactive modified COS (mCOS), which was site-specifically grafted onto SF chains via laccase-catalyzed oxidative coupling, followed by in situ mineralization. The resulting SF- g -mCOS hybrid scaffold exhibited enhanced calcium affinity, improved structural stability, and enabled controlled HAp deposition under alternating biomimetic mineralization conditions. The compressive stress of scaffolds increased from 7.13 kPa (SF) to 19.76 kPa (SF- g -mCOS), and further to 79.14 kPa after cyclic mineralization, accompanied by elevated phosphorus incorporation and significantly improved crystallinity and orientation of HAp. In vitro assays demonstrated that the objective scaffold showed superior cytocompatibility and accelerated osteogenic differentiation compared to native SF. This work provides a sustainable strategy for modifying SF with polysaccharides, achieving nanoscale interfacial mineralization and macroscale mechanical enhancement, thus offering a highly translatable platform for SF-based bone regeneration materials.
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CHEMICAL ENGINEERING JOURNAL
·
2026
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IF 12.5
Fengyi Li,Hong Lin,Chuan Zhang,Jing Li,Limin Cao,Jianxin Sui,Kaiqiang Wang,Lusheng Xin,Xiaokun Wang,Xiudan Wang
A single-enzyme level inorganic–organic engineering strategy was developed. • XOD@Si with enhanced activity and stability was achieved using silica precursors. • XOD@Si was integrated with pullulan on nitrocellulose membrane. • The XOD@Si-Pul biosensor achieve high stability under room temperature. • The XOD@Si-Pul biosensor enables on-site detectin of Hx in various food samples. Hypoxanthine (Hx) is an important biomarker for food freshness and clinical diagnosis, necessitating rapid and reliable on-site detection methods. Paper-based enzyme sensors offer a promising solution, yet their practical application is severely constrained by poor storage stability at room temperature. Herein, we report a bioinspired paper-based enzyme sensor for Hx detection based on enzyme stabilization at the single-enzyme level. Each xanthine oxidase (XOD) molecule was encapsulated within a conformal silica shell with three different silica precursors, achieving single-enzyme-level silicification and yielding XOD@Si with enhanced catalytic activity and structural stability. Further integration of XOD@Si with pullulan network on nitrocellulose membrane resulted in excellent color uniformity and signal intensity. The sensor exhibited a linear response of 19.5–625 μM and a limit of detection of 6.7 μM. With good selectivity and reliable quantitative capability, the sensor performed well in liquid meat soup and enabled “attach-and-read” assessment of meat freshness. Notably, the biomimetic dual-protection strategy conferred excellent storage stability, maintaining stable signal output for several weeks at room temperature, thereby overcoming a major limitation of conventional paper-based enzyme sensors. This work provides a versatile and scalable strategy for stabilizing enzymes on paper substrates, contributing to the development of stable, low-cost, and field-deployable biosensing platforms.
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高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2026
·
IF 4.1
Tian Juan,Yan Chunyu,Jiao Yuanyuan,Li Jiangbo,Chen Yaofeng,Chu Zongtang,Wu Yuxin,Liu Yanli,Zhou Zhe,Xing Yaling
DNA is widely employed in biological science and materials science, driving a growing demand for its effective preservation. The conventional cryopreservation method is highly resource-intensive, requiring substantial storage space and continuous energy input. These limitations highlight the urgent need to develop cost-effective and efficient alternative strategies. In this study, we creatively extended the application of chitosan derivatives to the preservation of extracellular DNA, based on our previous work on chitosan-mediated nucleic acid enrichment and detection. Among different types of chitosan derivatives, chitosan oligosaccharide lactate (COL) was identified for its outstanding DNA-binding and re-release capabilities. Upon complexation with COL, DNA is effectively protected against major environmental stressors including enzymatic hydrolysis, oxidative damage, and ultraviolet irradiation, which were the major environmental stressors. This protective interaction significantly enhances the stability of DNA under non-cryogenic conditions, which in turn minimizes the losses in DNA concentration and integrity while preserving critical sequence information. Furthermore, the binding and release of COL with DNA could be achieved handily by pH adjustment. Moreover, COL could be readily available and amenable to large-scale production. Collectively, our findings provide a great potential method for realizing convenient and cost-effective DNA preservation at room temperature. Graphical abstract
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CELLULOSE
·
2025
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IF 4.8
Tian Xiuzhi,Xiao Jiale,Bai Haozhe,Aissa Fatima Ait,Li Wei,Zhao Dou,Jiang Xue
A sustainable and scalable strategy for fabricating high-quality chito-oligosaccharide (COS)-functionalized boron nitride nanosheets (COS-g-BNNSs) was first developed via COS-assisted ball milling combined with ultrasonication. The method achieved an 85% yield of COS-g-BNNSs with an aspect ratio of ~ 400, layers of 10 ~ 15 and minimal structural defects. COS grafting (15 wt%) introduced hydrophilic -NH 2 and -OH groups, ensuring pH-independent aqueous stability (pH < 3 or > 4) through synergistic steric hindrance and electrostatic repulsion. The amphoteric nature of COS-g-BNNSs (pHzpc = 3.8) facilitated nanosheets’ homogeneous dispersion in carboxymethyl cellulose (CMC), forming vertically aligned thermal pathways via hydrogen bonding. The resulting CMC/COS-g-BNNSs nanocomposite films exhibited exceptional thermal and mechanical properties: a through-plane thermal conductivity of 2.44 W/(m·K) (60-fold higher than that of pure CMC), tensile strength of 16.5 MPa (302% improvement), and Young’s modulus of 41.1 MPa (856% enhancement), while retaining flexibility (48.3% elongation). Thermal management tests demonstrated a 20 °C reduction in the operating temperature of COS-g-BNNSs-incorporated circuits, alongside stable performance under cyclic bending. This eco-friendly approach utilized water-based purification and biodegradable components, positioning the COS-g-BNNSs/CMC nanocomposites as promising substrates for flexible electronics, balancing high thermal conductivity, mechanical robustness, and environmental sustainability.
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高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 24
·15735
·
IF 6.208
Zhe Luo,Yang Zhang
Wood dyeing is an effective way to alleviate the supply-demand imbalance of valuable wood and improve the surface decoration of fast-growing wood. However, applications of dyed wood are limited due to the susceptibility of dyes and wood to photo-discolor and degrade under light irradiation. Thus, the improved weather resistance of dyed wood is crucial. To prevent photochromic discoloration of dyed wood, an anti-photochromic coating structure was constructed via layer-by-layer self-assembly (LbL) using chitosan and zinc oxide (ZnO). The results showed that the surface color difference of treated dyed wood was reduced by approximately 84.6% after the first 2 h of irradiation under the following conditions °C: temperature (50 °C), relative humidity (55%), and irradiation intensity (550 W/m2). However, the color of untreated dyed wood drastically changed at this stage. The reason for the decrease was that the redness and yellowness of treated dye wood were significantly reduced. The deposition of ZnO onto treated dyed wood helped to protect the wood from UV light irradiation. Chitosan bridged the dyes and complexed ZnO to enhance UV resistance. This study provides valuable information for the protection of dyed wood against light discoloration that can be used as an interior and exterior decorative material.Keywords:dyed wood;UV;chitosan;ZnO;photochromic;assembly
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Chun-Xia Zhang,Yu Wang,Xinhe Duan,Kai Chen,Hong-Wei Li,Yuqing Wu
The cytidine 5′-monophosphate (CMP)-protected gold nanoclusters (AuNCs@CMP) was developed to be a fluorescent nanosubstrate for direct ratiometric determination of alkaline phosphatase (ALP). The binding mode investigations revealed that the ligand CMP binds to the gold core via two oxygen atoms of ribose and cytosine and leaves the phosphate group freely outside, promoting AuNCs@CMP to be the direct nanosubstrate of ALP. AuNCs@CMP emits at 570 nm while the hydrolysis product of AuNCs@Cyt emits at 485 nm; the large emission gap between them provided a ratiometric approach for ALP determination. Moreover, a natural and biofunctional oligomer , chitosan oligosaccharide (COS), was introduced to the system, which significantly amplified the fluorescence signals of AuNCs@Cyt and improved the limit of detection (LOD) down to 0.00026 U·L −1 . Furthermore, the intrinsic mechanism of AuNCs@CMP hydrolysis by ALP and the COS amplification on AuNCs@Cyt were studied in-depth, which indicated the emission enhancement was attributed to the aggregation-induced emission enhancement (AIEE) property of the produced AuNCs@Cyt. Finally, the developed approach was successfully applied to determine ALP in human serum and the evaluation of ALP inhibitors. Therefore, the present study develops a novel nanosubstrate for ALP determination in a range of 0.0050–0.25 U·L −1 and provides a straightforward way to amplify the fluorescence signal by employing available polymer. The work will stimulate and encourage the structural promotion of metal nanoclusters, and extend their biological applications broadly.
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危险品化学品经营许可证（带存储）