ANALYTICA CHIMICA ACTA
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2026
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IF 6.1
Yali Yang,Hui Ding,Kaijun Quan,Hongwei Zhao,Mingxia Sun,Zhimin Yang,Xiuhui Liu,Hongdeng Qiu,Jia Chen
Background To address the limitations of traditional single-mode stationary phases in separating complex samples, mixed-mode or multi-mode chromatographic stationary phases, which can provide two or more interaction mechanisms, have attracted extensive research interest. Among them, sulfonated pyrene derivatives present a promising candidate ligand, as they synergistically retain the hydrophobicity and π-π interaction capability of the pyrene moiety along with the hydrophilicity and strong cation-exchange capacity provided by the sulfonic acid groups. This synergistic multi-mechanism design significantly enhances separation selectivity for diverse and complex components. Results A pyrene trisulfonic acid-functionalized silica stationary phase (Sil-PyTSa) was successfully prepared and evaluated. In hydrophilic interaction liquid chromatography (HILIC) mode, Sil-PyTSa could efficiently separate nucleobases and nucleosides, ginsenosides, and alkaloids. For nucleobases and nucleosides, it utilized hydrophilic, electrostatic, and π-π interactions, showing superior retention and selectivity over a control HILIC column. In reversed-phase liquid chromatography (RPLC) mode, favorable separation of PAHs was achieved via hydrophobic and π-π interactions, enabling faster analysis and lower solvent consumption than a C18 column. In ion-exchange chromatography (IEC) mode, baseline separation of 14 rare earth elements (REEs) and removal of Fe/U impurities were accomplished. The column efficiency reached 95,920 N/m for naphthalene. Additionally, Sil-PyTSa achieved effective separation for REEs and iron (Fe) in a simulated NdFeB leaching solution and detected nucleobases and nucleosides in Angelica, indicting promising potential for analyzing complex matrices. Significance and novelty This work developed a novel multi-mode chromatographic stationary phase with multiple interactions mechanisms to enable high-performance separations in HILIC, RPLC, and IEC modes. It demonstrates good separation efficiency and selectivity, low solvent consumption, and high stability. This offers a versatile and green solution for the separation of complex samples with diverse analytes.
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Science Advances
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2026
·Vol. 12
·期 33
·
IF 13.9
Siyu Wang,Tingting Zhao,Wanying He,Fei Li,Zhicheng Wang,Ruhao Pan,Lianfu Li,Shichuan Xi,Zhendong Luan,Zhiyang Zhang,Bowei Li,Lingxin Chen,Xin Zhang
Deep-sea hydrothermal vents and cold seeps, typical ecosystems characterized by abundant carbon but limited nitrogen, support thriving microbial activities. The source of nitrogen has long been a key focus in scientific research. Although cultivation experiments have confirmed the presence of diverse nitrogen-fixing microorganisms, direct in situ evidence has been lacking. Therefore, a deep-sea in situ surface-enhanced Raman scattering sensor has been developed. It can adapt to extreme environment (≥350°C and ≥2000-m depth and LOD < 10 −7 M) and has excellent deep-sea application potential. Crucially, the sensor achieved the in situ detection of cyanide (-CN) (>5.7 μM) at cold seep, providing direct evidence for an energy-efficient nitrogen fixation pathway in the microbial communities. Concurrently, the gradient detection results indicated that CN was consumed as a microbial “circulating currency.” This finding offers critical in situ evidence for understanding the coupling mechanisms of cold seep carbon, nitrogen, and sulfur cycles, marking a substantial breakthrough in deep-sea sensing technology.
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BIOCHEMICAL SYSTEMATICS AND ECOLOGY
·
2026
·
IF 2.2
Qiucheng Luo,Gangxiang Fei,Xiaoguang Tian,Shuang Han,Jingqiang Guan,Bo Song,Longzhan Gan,Yeming Zhou,Zhijie Wang,Hongbo Lan,Xiao Zou
Two chemotypes, CTA and CTB, were identified in Cordyceps chanhua for the first time. • Intracellular nucleosides were key markers of chemotype differentiation. • CTA showed significantly higher intracellular uridine, guanosine, adenosine, and HEA than CTB. • Altitude and precipitation were the major environmental factors associated with chemotype differentiation. • Intracellular nucleosides showed significant negative correlations with altitude and precipitation and strong positive correlations with each other. Cordyceps chanhua is a valuable medicinal and edible fungus with great potential for functional food development because of its bioactive metabolites with significant pharmacological activities. However, samples from different geographical origins show significant variations in metabolite composition and content. Existing studies are mostly limited to local regions and lack systematic evaluation based on multi-regional samples, particularly regarding chemotype classification and its environmental driving mechanisms. Therefore, this study systematically analyzed the differences in bioactive metabolites, chemotype differentiation patterns, and their environmental driving factors using multi-regional samples. A total of 123 C. chanhua samples were collected from 16 regions across 8 provinces in China. Key bioactive metabolites, including uridine, guanosine, adenosine, N6-(2-hydroxyethyl)-adenosine (HEA), cordycepic acid, extracellular polysaccharides, and ergosterol, were determined in liquid fermentation broth. The results showed significant geographical differences in metabolite accumulation. Hierarchical cluster analysis (HCA) and principal component analysis (PCA) classified the samples into two chemotypes, Chemotype A (CTA) and Chemotype B (CTB). CTA exhibited significantly higher intracellular nucleoside contents than CTB ( p < 0.01), and intracellular nucleosides were identified as the core variables driving chemotype differentiation. Environmental analysis showed that CTA was mainly distributed in low-altitude and low-precipitation regions, while intracellular nucleosides were significantly negatively correlated with altitude and precipitation. Correlation network analysis further revealed synergistic accumulation patterns among multiple metabolites. This study revealed the geographical variation characteristics of bioactive metabolites in C. chanhua , identified its chemotypes for the first time, and clarified that intracellular nucleosides are key chemical markers for chemotype classification. Download: Download high-res image (340KB) Download: Download full-size image
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Bo Wang,Feng Zhao,Zheng Zhou,Ji Wang,Lei Qin,Xuhui Huang
Studied hazardous compound changes in the thermal processing of 7 animal foods. • Hazards: acrylamide, 5-hydroxymethylfurfural, 13 heterocyclic aromatic amines. • AA, 5-HMF, and 13 HAAs in all animal foods increased with roasting temperature. • Low moisture and high carbs promoted AA; high protein favored HAAs. • Amino acids and dipeptides had negative correlations with hazardous compounds. The thermal processing of food is often accompanied by changes in composition and the generation of hazardous compounds. However, the unclear mechanisms linking food composition to related hazardous compounds hinder the advancement of inhibition technologies for hazardous compounds formed during thermal processing. In this paper, the mechanisms of 15 kinds of associated hazardous compounds produced by 7 different animal-derived food samples during the roasting process were investigated. When the roasting temperature was 260 °C, mutton exhibited the highest acrylamide content at 0.11 μg/g, chicken had the highest HAAs (heterocyclic aromatic amines) content at 0.17 μg/g, and Antarctic krill showed the highest 5-hydroxymethylfurfural content at 0.72 μg/g. notably, the compounds closely related to the production of associated hazards during thermal processing include amino acids, small dipeptides, and certain carbohydrate compounds. The results of this study have positive significance in improving food safety and optimizing production processes. Download: Download high-res image (136KB) Download: Download full-size image
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JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Minyi Yuan,Zheng Zhao,Ji Wu,Sumin Bian,Yuling Liao,Ying Zhou,Huikai Shao,Zhengjin Jiang,Qiqin Wang
To meet the increasing demand for the separation of complex samples, a novel monolithic stationary phase with hydrophobic and hydrophilic functionalities was developed by using 3-(1-(4-vinylbenzyl)-1H-imidazol-3-ium) propane-1-sulfonate (VBIPS) as monomer, pentaerythritol triacrylate (PETA) as crosslinker. The optimized mixed-mode poly(VBIPS- co -PETA) monolith exhibited good mechanical strength, high permeability (K = 0.23 × 10 -14 m 2 in 100% ACN, 0.23 × 10 -14 m 2 in 100% MeOH, and 0.11 × 10 -14 m 2 in 100% H 2 O), and multiple interaction modes, including hydrophilic, hydrophobic, hydrogen, and electrostatic interactions. The highest column efficiency of 83,330 plates/m was achieved for thiourea at a linear velocity of 0.23 mm/s. Systematic evaluations confirmed strong retention and separation of polar substances (e.g., benzoic acids, nucleoside bases, phenols), primarily due to the sulfobetaine-derived hydrophilicity as well as effective separation of nonpolar substances (e.g., aniline, aryl ketones, polyaromatic hydroxy compounds), facilitated by π-π stacking interactions between analytes and benzene rings within the stationary phase. Overall, this zwitterionic imidazolium sulfonate-functionalized monolith successfully combines hydrophobic and hydrophilic groups in a single continuous bed, enabling efficient separation of both polar and nonpolar analytes under low backpressure. This work enriches the library of mixed-mode stationary phases, and broadens the detection scope for complex sample analysis.
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Pharmaceutics
·
2022
·Vol. 14
·期 11
·2307
·
IF 6.525
Xueyan Gou,Fenglin Ran,Jinru Yang,Yanrong Ma,Xin’an Wu
Background: Organic anion transporter 1 (OAT1) and OAT3 have an overlapping spectrum of substrates such that one can exert a compensatory effect when the other is dysfunctional. As a result, the knockout of either OAT1 or OAT3 is not reflected in a change in the excretion of organic anionic substrates. To date, only the mOAT1 and mOAT3 individual knockout mouse models have been available. Methods: In this study, we successfully generated aSlc22a6/Slc22a8double-knockout (KO) rat model using CRISPR/Cas9 technology and evaluated its biological properties. Results: The double-knockout rat model did not expressionmRNAfor rOAT1 or rOAT3 in the kidneys. Consistently, the renal excretion ofp-aminohippuric acid (PAH), the classical substrate of OAT1/OAT3, was substantially decreased in theSlc22a6/Slc22a8double-knockout rats. The relativemRNAlevel ofSlco4c1was up-regulated in KO rats. No renal pathological phenotype was evident. The renal elimination of the organic anionic drug furosemide was nearly abolished in theSlc22a6/Slc22a8knockout rats, but elimination of the organic cationic drug metformin was hardly affected. Conclusions: These results demonstrate that this rat model is a useful tool for investigating the functions of OAT1/OAT3 in metabolic diseases, drug metabolism and pharmacokinetics, and OATs-mediated drug interactions.Keywords:OAT1/OAT3;CRISPR/Cas9;gene knockout;pharmacokinetic
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Advanced Science
·
2023
·Vol. 10
·期 9
·2206851
·
IF 15.1
Peidong Du,Yanzhe Shen,Baoli Zhang,Shan Li,Minzheng Gao,Ting Wang,Xiaokang Ding,Bingran Yu,Zhen-Gang Wang,Fu-Jian Xu
Photodynamic therapy (PDT) is a light triggered therapy by producing reactive oxygen species (ROS), but traditional PDT may suffer from the real-time illumination that reduces the compliance of treatment and cause phototoxicity. A supramolecular photoactive G-quartet based material is reported, which is self-assembled from guanosine (G) and 4-formylphenylboronic acid/1,8-diaminooctane, with incorporation of riboflavin as a photocatalyst to the G4 nanowire, for post-irradiation photodynamic antibacterial therapy. The G4-materials, which exhibit hydrogel-like properties, provide a scaffold for loading riboflavin, and the reductant guanosine for the riboflavin for phototriggered production of the therapeutic H 2 O 2 . The photocatalytic activity shows great tolerance against room temperature storage and heating/cooling treatments. The riboflavin-loaded G4 hydrogels, after photo-irradiation, are capable of killing gram-positive bacteria (e.g., Staphylococcus aureus ), gram-negative bacteria (e.g., Escherichia coli ), and multidrug resistant bacteria (methicillin-resistant Staphylococcus aureus ) with sterilization ratio over 99.999%. The post-irradiated hydrogels also exhibit great antibacterial activity in the infected wound of the rats, revealing the potential of this novel concept in the light therapy.
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Genes
·
2022
·Vol. 13
·期 5
·878
·
IF 4.141
Xiuying Lin,Qianhui Zhang,Yichao Qin,Qisheng Zhong,Daizhu Lv,Xiaopeng Wu,Pengcheng Fu,Huan Lin
Triple quadrupole mass spectrometry coupled to liquid chromatography (LC-TQ-MS) can detect and quantify modified nucleosides present in various types of RNA, and is being used increasingly in epitranscriptomics. However, due to the low resolution of TQ-MS and the structural complexity of the many naturally modified nucleosides identified to date (>160), the discrimination of isomers and mass-analogs can be problematic and is often overlooked. This study analyzes 17 nucleoside standards by LC-TQ-MS with separation on three different analytical columns and discusses, with examples, three major causes of analyte misidentification: structural isomers, mass-analogs, and isotopic crosstalk. It is hoped that this overview and practical examples will help to strengthen the accuracy of the identification of modified nucleosides by LC-TQ-MS.Keywords:nucleoside misidentification;RNA modification;epitranscriptomics;LC-MS/MS;tRNA;HILIC
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JOURNAL OF APPLIED PHYCOLOGY
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2022
·Vol. 34
·期 3
·1497-1511
·
IF 3.404
Liu Jing,Bai Xinpeng,Fu Pengcheng
Arthrospira platensis proteins are considered as viable ingredients for functional foods with nutraceutical properties and health-promoting effects. In this study, we used five different proteases including pepsin, trypsin, alcalase, papain, and bromelain to hydrolyze A. platensis phycobiliprotein. LC–MS/MS (liquid chromatography-tandem mass spectrometry) was used to determine the composition of the hydrolysates with thousands of bioactive peptides. Based on peptide sequencing, a candidate list of 1,333 bioactive peptides was constructed for the first time for which bioinformatics tools were used to assess the properties of the bioactive peptides. The inhibitory activity of dipeptidyl peptidase IV (DPP-IV) and angiotensin-converting enzyme (ACE), as well as the antioxidant activity of five phycobiliprotein hydrolysates (PBPHs), were verified in vitro. The IC 50 values of PBPH-pepsin, PBPH-trypsin, PBPH-alcalase, PBPH-papain, and PBPH-bromelain for DPP-IV inhibited activity were determined to be 4.059, 5.603, 5.257, 3.819, and 4.195 mg mL −1 , respectively. All the above five PBPHs were also seen to significantly inhibit ACE activity ( P < 0.0001) in the range of 0.1–1.0 mg mL −1 . The activity of five PBPHs fraction was found for the 2-azino-bis (3-ethylbenzothiazoline6-sulfonic acid) diammonium salt (ABTS) assay (the highest was 1.37 mM Trolox equivalent of 30 mg mL −1 of PBPH-trypsin), 1,1diphenyl-2-picrylhydrazyl (DPPH) assay (the highest was 204.67 µM Trolox equivalent of 10 mg mL −1 of PBPH-pepsin), and Fe 2+ chelating ability (the highest was 639.73 µM FeSO 4 equivalent of 10 mg mL −1 of PBPH-trypsin). This study indicated that it was feasible to utilize A. platensis phycobiliprotein as a source of bioactive peptides which may be used as functional food and/or nutritional supplements for human health.
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JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Kaixing Luo,Yu Luo,Yanjuan Liu,Yuefei Zhang,Wei Chen,Zhengwu Bai,Sheng Tang
s In this work, a new strategy of simultaneously grafting two different functional ligands onto the silica surface was adopted to prepare a carbonyldiimidazolium/dodecyl dual-functionalized silica stationary phase (Sil-CDI-DD) by recycling the discarded commercial C18 columns as the raw materials. A full characterization of the waste silica-C18, the calcined silica, the acidified silica and the new packing materials was done to explore the feasibility of recycling the waste chromatographic columns. Hydrophobic and hydrophilic selectivity of the obtained Sil-CDI-DD was specifically investigated, and retention mechanisms in reversed-phase and hydrophilic interaction chromatography modes were discussed. The experimental results showed that the perfect combination of carbonyldiimidazolium and long-chain alkyl groups commendably enhanced the separation selectivity and efficiency of the versatile Sil-CDI-DD, which could provide multiple interaction sites for diverse target analytes, thereby contributing to the favorable resolution of hydrophilic nucleosides/nucleobases and weak polar environmental endocrine disruptors , including alkylphenols, bisphenols , steroid hormones and phthalates .
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MOLECULES
·
2022
·Vol. 27
·期 13
·4130
·
IF 4.927
Qiuhua Zhang,Lingyi Huang,Youjia Wu,Liying Huang,Xiaowen Xu,Renyi Lin
CompoundAnoectochilus roxburghii(Wall.) Lindl. (A. roxburghii) oral liquid (CAROL) is a hospital preparation ofA. roxburghiiandGanoderma lucidum(G. lucidum),which have hepatoprotective effects. Eight active components (five nucleosides/nucleobases and three triterpenoid acids) in CAROL,A. roxburghii, andG. lucidumwere simultaneously detected by high-performance liquid chromatography–tandem mass spectrometry (LC–MS/MS). The multiple reaction monitoring (MRM) mode was applied for the detection of analytes. These eight compounds were separated well within 12 min and quantified using the internal standard working curve method. The method showed good linearity (R2> 0.9935) and high sensitivity (limit of detection = 0.29 ng/mL). The analyte recovery ranged from 85.07% to 97.50% (relative standard deviation < 3.31%). The content of the target analytes in four batches of CAROL, and the raw materials ofG. lucidumandA. roxburghiifrom the five regions was determined using this method. The contents of guanosine and ganoderic acid A in four batches of oral liquid were high and stabilized and could be recommended as quality markers (Q-marker) for CAROL. Simultaneous qualitative and quantitative analysis of nucleosides and triterpenoid acids in CAROL,A. roxburghii, andG. lucidumby LC–MS/MS based on the MRM model was reported for the first time. The proposed method provides a sensitive, rapid, and reliable approach for the quality control of Chinese medicinal products.Keywords:LC–MS/MS;Anoectochilus roxburghii(Wall.) Lindl. and its compound oral liquid;Ganoderma lucidum;analysis;quality control
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JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Shitao Feng,An Zhang,Fengshou Wu,Xiaogang Luo,Juan Zhang
This study constructed boronic acid grafted Zr-MOF (BA-Zr-MOF) by a simple pre-installation strategy through mixed organic ligands. Typically, BA-Zr-MOF was prepared by one-step hydrothermal method used for enrichment of cis -diol-containing nucleosides through pipette tip solid-phase extraction (PT-SPE) followed by detection of high-performance liquid chromatography. It is worth mentioning that BA is well assembled into MOF and cis -diol-containing compounds can be efficiently and selectively enriched by abundant boronic acid groups. Three groups of different types of compounds were used to evaluate their selectivity and the results showed the excellent selectivity to cis -diol-containing compounds of as-prepared adsorbent. The BA-Zr-MOF adsorbent possesses a high adsorption capacity, which can reach 86.40 mg g −1 for adenosine. Under the optimal extraction condition, a PT-SPE-HPLC method based on BA-Zr-MOF for analysis of nucleosides was established. The linear range of the four nucleosides is 0.01 to 50 µg mL −1 with R 2 ≥ 0.99 and the detection limits (LODs) are estimated at between 0.005-0.012 µg mL −1 . The recoveries in urine were used to test the reliability of the analytical methods, which ranged from 82.8% to 117.1%, with intra-day relative standard deviations (RSDs) ranged from 0.1% to 4.2% and the inter-day RSDs ranged from 0.2% to 6.2%. All the results show that the pre-installation strategy based on dual ligands is an alternative to fabricate MOF composite material and BA-Zr-MOF is a promising material for the analysis of cis -diol-containing biomolecules.
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