JOURNAL OF FOOD SCIENCE
·
2024
·Vol. 89
·期 12
·8398-8409
·
IF 3.2
Junguo Wang,Hongyue Wang,Keshuang Guo,Xindi Zhang,Xiangrong Zhang
The purpose of this study was to prepare and evaluate the formulation of nanoemulsions (NEs) to encapsulate phloretin (PT) to improve its stability, antioxidant, and tyrosinase inhibitory competence. The aim of this study was to improve the stability, antioxidant, and tyrosinase inhibitory effects of PT via NEs. The formulations were prepared using low energy emulsification method for PT-VE-NEs, α -tocopherol (Vitamin E) and medium chain triglycerides (MCT) were used as the oil phase, and Tween 60 was used as the emulsifier and PEG-400 as the co-emulsifier. The droplet size and zeta potential of oil-in-water NEs were evaluated using dynamic light scattering. The PT-VE-NEs were also characterized by transmission electron microscopy and Fourier transform infrared spectroscopy. The mean droplet diameter was 14.85 ± 0.14 nm, with a zeta potential of −2.47 ± 0.51 mV. Fourier transform infrared spectroscopy revealed the formation of molecular interactions in the NEs formulations. PT-VE-NEs size was maintained the same during the in vitro digestion study. The particle size of PT-VE-NE remained stable during in vitro digestion. The addition of VE significantly improved the antioxidant, tyrosinase inhibitory effects, as well as thelion and physical stability of PT-VE-NE. The results revealed that NEs is a promising strategy to improve the functionality and stability of PT and VE. PT-VE-NEs will be applied for the preservation of fruits.
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高影响因子
CROP PROTECTION
·
2026
·
IF 3.3
Yuying Xie,Yangchun Ge,Zhuochao Shen,Yupeng Qiu,Haohua Zhang,Changmeng Sun,Jiangkuan Tang,Bingke Li,Junran Xu,Ping Liang,Yubang Tao,Shengtian Wang,Xuqiang Peng,Zhongyu Li
Hydrophobic herbicides often suffer from poor solubility and low bioavailability in crop protection applications, which hinders the sufficient uptake of active ingredients by target weeds and severely limits their effectiveness in safeguarding crop growth. To enhance the delivery efficiency and targeting capability of such herbicides, this study developed a pH-responsive amphiphilic polymer nanocarrier using the natural product kojic acid and triethylene glycol divinyl ether as raw materials for loading diuron. This carrier can self-assemble in aqueous media to form stable core-shell nanoparticles with a critical micelle concentration of 6.92 × 10 −3 mg/mL and an average particle size of 181.4 nm after drug loading. In a simulated acidic rhizosphere environment of Echinochloa crus-galli , the drug-loaded nanoparticles exhibited distinct pH-responsive release behavior while maintaining good physical stability. Biological experiments further confirmed that this delivery system significantly enhances the herbicidal activity of diuron against Echinochloa crus-galli , providing new insights and a material foundation for the development of efficient and intelligent herbicide delivery systems.
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Pharmaceuticals
·
2026
·Vol. 19
·期 3
·469
·
IF 5.7
Lili Li,Xiaoya Zhang,Guangyuan Tang,Jianxin Wu,Qing Huang
Background: Glabridin, a natural compound derived fromGlycyrrhiza glabraL., possesses skin-lightening effects. This study aims to further elucidate the depigmentation mechanism of glabridin by investigating its effects on melanogenesis and melanin transfer.Methods: We initially confirmed the anti-melanogenic effects of glabridin in MNT-1 human melanoma cells. Then, we investigated the mechanism of its anti-melanogenic effect by evaluating the protein expression of β-catenin and MITF via Western blot. To investigate melanin transfer, we compared glabridin’s efficacy with that of niacinamide, a recognized inhibitor of melanosome transfer and employed two complementary experimental models: (1) α-melanocyte-stimulating hormone (α-MSH)-stimulated MNT-1 cells to analyze dendrite formation, and (2) a UVB-irradiated co-culture system of MNT-1 cells and HaCaT keratinocytes to evaluate melanin transfer.Results: By measuring glabridin’s effects on melanin content, tyrosinase activity, and melanogenesis-related protein expression confirmed its inhibition of melanin synthesis. Further investigation demonstrated that glabridin suppresses melanogenesis by downregulating β-catenin and MITF, indicating inhibition of the Wnt/β-catenin pathway. Furthermore, in α-MSH-treated MNT-1 cells, both glabridin and niacinamide were found to suppress dendrite formation and elongation. In a UVB-exposed co-culture system, both glabridin and niacinamide inhibited melanin transfer to keratinocytes. Mechanistically, these effects were linked to the regulation of Rho GTPases (Rac1, RhoA, Cdc42) and suppression of F-actin reorganization.Conclusions: This study provides, for the first time, evidence that the skin-lightening effect of glabridin involves two complementary mechanisms: inhibition of melanogenesis through suppression of the Wnt/β-catenin pathway, and attenuation of both dendricity and melanin transfer via the influence of Rho family GTPases expression.
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高影响因子
Foods
·
2026
·Vol. 15
·期 17
·3045
·
IF 6
Feifei Wang,Yuxuan Duan,Ziying Cai,Shidong Lin,Lingjun Yu,Yongbiao Zheng
Six representative small-molecule constituents were isolated and structurally characterized from the edible mushroomTremella fuciformis:cis-oleic acid (1), 1-oleoylglycerol (2), cerevisterol (3), a cerebroside (4),trans-homoaconitic acid (5), and uridine (6). Compound5, a tricarboxylic acid derivative, is reported for the first time as abundantly accumulated in this fungus. Aging-relevant assays showed that 1 potently inhibited α-glucosidase (IC50= 0.02 mM, surpassing acarbose) and moderately inhibited acetylcholinesterase (IC50= 0.29 mM);5competitively inhibited alkaline phosphatase (IC50= 5.47 mM) and suppressed GluN2A-containing NMDA receptor currents (IC50= 0.40 mM), the first such dual report for a fungal tricarboxylic acid; and4inhibited tyrosinase (IC50= 0.80 mM) comparably to kojic acid. HPLC confirmed meaningful recovery under mild hydro-alcoholic extraction compatible with functional food processing. These multifunctional small molecules target carbohydrate metabolism, cognition, and skin homeostasis, supportingT. fuciformisas a source of healthy-aging food ingredients.
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JOURNAL OF MOLECULAR STRUCTURE
·
2025
·
IF 4.7
Chuang Liu,Ying Li,Yu Zhang,Shiyu Li,Jun Dang
The discovery of tyrosinase modulators from natural products has long been hampered by limitations in screening efficiency and accuracy. In this study, a novel online screening system for tyrosinase was constructed based on HPLC technology that enables high-throughput recognition of active constituents in complex matrices. Applying this HPLC-tyrosinase system to Dracocephalum heterophyllum ( D. heterophyllum ), three active peaks were precisely captured, which were subsequently isolated and structurally characterized as caffeic acid, verbascoside, and rosmarinic acid. Enzyme activity assays revealed that caffeic acid (IC 50 = 1.08 mM) and rosmarinic acid (IC 50 = 0.41 mM) markedly inhibited tyrosinase activity, whereas verbascoside exhibited activating properties (EC 50 = 0.24 mM). The action mechanisms and bioactivities of these compounds were further elucidated through density functional theory calculations, molecular docking analysis, and in vivo validation. Collectively, the online HPLC-tyrosinase activity screening system established in this work not only provides a theoretical basis for the high value-added development of D. heterophyllum , but also delivers a high-throughput, precision tool for discovering natural tyrosinase inhibitors, demonstrating robust application potential in the fields of cosmetic science, food preservation and pharmaceutical development.
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Food Bioscience
·
2025
·
IF 5.9
Jindan Zhang,Deyang Yu,Jun Zhao,Yiping Chen,Mingqian Tan
Utilizing natural colorant-based colorimetric sensors for food freshness monitoring is an effective approach to reduce food waste and foodborne illness. However, it is hard to modulate the colorimetric endpoint of the pH-responsive natural colorants to determine the food quality accurately. Herein, Kojic acid (KA)-modulated anthocyanin (ACN)/ hydroxypropyl methylcellulose (HPMC)-sodium alginate (SA) film-based colorimetric sensor was prepared using drop casting method. HPMC and SA provided a homogeneous framework to incorporate ACN. KA, a natural product derived from fermented foods, was introduced to manipulate the colorimetric endpoint of ACN to realize an accurate prediction for the freshness of salmon. Molecular dynamics (MD) simulations and density functional theory (DFT) revealed not only the molecular interactions among KA, ACN, HPMC, and SA, but also the strong interactions between the KA-ANC/HPMC-SA colorimetric sensor and trimethylamine (TMA). Results of UV-vis spectra and TMA response experiments illustrated that the KA can inhibit the deprotonation process and promote the hydration equilibrium of ACN, which makes the KA-ACN/HPMC-SA colorimetric sensors exhibit different color responses comparing to the pristine ACN/HPMC-SA. Among all colorimetric sensors, the 1KA-ACN/HPMC-SA exhibited the most sensitive response to TMA with a limit of detection (LOD) of 15.79 μM. When applied in the real-time monitoring of the freshness of salmon at 4°C, the 1KA-ACN/HPMC-SA showed the most significant total color difference (ΔE) of 31.28±0.76 and synchronous color change with the spoilage of salmon after 10 days. The degradation and seed germination experiments of the colorimetric sensors indicated that the colorimetric sensors were environmentally degradable and had good biosafety. This work proposes a novel and effective strategy to modulate the colorimetric endpoint of ACN, and the prepared colorimetric sensors show applicable potential in the quality monitoring of salmon.
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JOURNAL OF MOLECULAR STRUCTURE
·
2023
·
IF 3.8
Jiangtao Su,Dan Li,Yuchen Hu,Xiangyu You,Xiaoxia Guo,Xican Li,Ban Chen
To improve the application prospects of celastrol (CE), a novel C 6 -sulfonated CE analog (called CENFN in this study) was designed via quantum chemical calculations. On this basis, CENFN was synthesized and clarified by spectroscopic methods (IR, 1 H NMR, UV–Vis and fluorescence spectra). Subsequently, the tyrosinase and melanin inhibitory effects of CE and CENFN were comparably explored in silico and in vitro . The calculation and spectroscopy results confirmed the rationality of the synthetic reactions of CENFN. The molecular simulation results showed that CENFN possessed a higher level of tyrosinase and melanin inhibition than CE, which was consistent with the experimental results. All these findings can help to design and synthesize CE analogs as tyrosinase and melanin inhibitors.
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JOURNAL OF SEPARATION SCIENCE
·
2023
·Vol. 46
·期 14
·2300195
·
IF 3.1
Yue Li,Xin-Xin Jiang,Hong-Mei Liu,Xiao-wei Li,Xiao-Jun Luo,Xun Liao,Yan Zhao
The inhibition of tyrosinase is considered to be a common therapeutic strategy for some hyperpigmentation disorders. Screening of tyrosinase inhibitors is of great significance to the treatment of pigmentation diseases. In this study, tyrosinase was covalently immobilized on magnetic multi-walled carbon nanotubes for the first time, and the immobilized tyrosinase was applied for ligand fishing of tyrosinase inhibitors from complex medicinal plants. The immobilized tyrosinase was characterized by transmission electron microscopy, atomic force microscopy, Fourier-transform infrared spectroscopy, vibrating sample magnetometry, and thermo-gravimetric analyzer, which indicated that tyrosinase was immobilized onto magnetic multi-walled carbon nanotubes. The immobilized tyrosinase showed better thermal stability and reusability than the free one. The ligand was fished out from Radix Paeoniae Alba and identified as 1,2,3,4,6-pentagalloylglucose by ultra-performance liquid chromatography-quadrupole time-of-flight high-resolution mass spectrometry. 1,2,3,4,6-pentagalloylglucose was found to be a tyrosinase inhibitor with similar half maximal inhibitory concentration values of 57.13 ± 0.91 μM compared to kojic acid (41.96 ± 0.78 μM). This work not only established a new method for screening tyrosinase inhibitors but also holds considerable potential for exploring the new medicinal value of medicinal plants.
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高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Min He,Meiyan Fan,Wei Yang,Zhiyun Peng,Guangcheng Wang
A class of new kojic acid hybrids ( 7a-7o ) bearing a 1,2,4-triazine moiety were prepared, and their inhibitory activities and mechanism on tyrosinase were investigated. All derivatives showed good to excellent anti-tyrosinase activity with IC 50 values ranging from 0.34 ± 0.06 μM to 8.44 ± 0.73 μM. In kinetic study, compound 7m was a mixed-type inhibitor with K i and K is of 0.73 and 1.27 μM, respectively. The interaction mechanism toward tyrosinase of compound 7m was further elaborated in combination with molecular docking and various spectral techniques. The results showed that compound 7m could change the secondary structure of tyrosinase to reduce its catalytic activity. Anti-browning assays demonstrated that 7m inhibited the browning of bananas effectively during storage. What’s more, 7m was found to have low cytotoxicity in vitro . In conclusion, compound 7m has the potential to be applied as an anti-browning agent.
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高影响因子
JOURNAL OF FOOD SCIENCE
·
2023
·Vol. 88
·期 3
·977-987
·
IF 3.9
Wenfu Hou,Yile Lin,Yujie Zhang,Tingting Liu,Juan Du,Yang Yi,Hongxun Wang
This study investigated the effect of 0.8% (m/v) kojic acid treatment on changes in the microstructure and myofibrillar protein of duck meat covered with oxygen-permeable polyvinylchloride (PVC) film (9 ± 0.5 µM) during superchilled storage (−1.65 ± 0.5°C). The superchilled samples exhibited wider gaps between muscle fibers at 5 weeks storage compared with kojic acid–treated groups. Based on the variation of water status, the water-holding capacity decreased significantly ( p < 0.05), and bound water and immobilized water were gradually converted into free water during superchilled storage. For kojic acid–treated samples, however, no major changes were observed with respect to muscle structure, water status, and protein degradation at 6 weeks. The 0.8% (m/v) kojic acid treatment increased the water-holding capacity, reduced carbonyl content and protein degradation, and decreased the α-helix contents loss of myofibrillar proteins. Kojic acid treatment effectively protected myofibrillar protein structure from being destroyed during superchilled storage, suggesting that this method was a good way to reduce protein oxidation and prolonged its shelf life.
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高影响因子
CHEMISTRY & BIODIVERSITY
·
2023
·Vol. 20
·期 3
·e202201171
·
IF 2.9
Qin-Tao Zhou,Peng-Yun Yang,Quan-Yu Yang,Wen-Hui Pang,Xiao-Li Li,Xing-Jie Zhang,Rui-Han Zhang,Wei-Lie Xiao
One new alkaloid, picrasine A, two new quassinoids, picralactones A−B, together with eleven known compounds were isolated from Picrasma chinensis P.Y. Chen. The structures of these compounds were determined using 1D and 2D NMR, HR-ESI-MS, and IR spectroscopic data, and by comparison with published data. Some compounds were tested for tyrosinase inhibiting activity, however, none of them exhibited strong inhibitory effects. Graphical
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高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2023
·
IF 1.2
Jianjin GUO,Lu BAI,Chi-Tang HO,Sen GUO,Naisheng BAI
Fraxinus mandshurica is widely used in folk medicine to treat various diseases and has significant pharmacological effects. To make full use of this resource, its roots were studied. Twelve lignans were first obtained by natural product extraction, selective separation and identification, and were identified and quantified by high performance liquid chromatography-photodiode array (HPLC-DAD). The results of quantitative analysis showed that the highest lignin content in F. mandshurica roots was compound 9 with 102.02 mg/g, while the overall lignin content in Shanxi was low. The results of methodological validation showed that the RSD values of intra-day precision ranged from 0.35% to 1.38%, while the RSD values of inter-day precision were within 1.97%; the RSD values of reproducibility experiments ranged from 0.35% to 1.96%, while the RSD values of stability experiments were less than 2.97%; the spiked recovey of samples were within 102.84%, and the RSD values ranged from 0.39% to 1.77%, which fully confirmed the high precision and good reproducibility of the analytical method. In addition, fingerprinting and heatmap were established for 40 batches of samples from 8 origins. Next, multivariate analysis, including principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA), was performed to investigate the differences in lignin content in the roots of F. mandshurica from different origins, and four lignins that could be used to distinguish F. mandshurica from different origins were screened. Finally, 12 lignans were evaluated for enzyme activity inhibition with α-glucosidase, tyrosinase and acetylcholinesterase, and the results showed that all 12 lignans exhibited enzyme activity inhibition to different degrees, which have potential applications in anti-diabetic, whitening and light spot and anti-Alzheimer's disease, and become a new source of natural enzyme activity inhibitors. This study established an identification and evaluation method applicable to plants of different origins, which provides a certain theoretical basis for the comprehensive exploitation of F. mandshurica .
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高影响因子
危险品化学品经营许可证（带存储）