Zhihao Wu,Ruchun Chen,Shuang Pan,Hui Liu,Xiaoli Hu
Herein, a convenient method is developed for ratiometric fluorescence detection of ATP (adenosine triphosphate) based on CDs-OPD (carbon dots- o-phenylenediamine). In the absence of ATP, oxOPD, the colored oxidation product of OPD fabricated by a redox reaction occurred between OPD and Cu 2+ , not only quenches the fluorescence of CDs, but also emits a yellow fluorescence which acts as another signal element. However, ATP will compete with OPD to chelate Cu 2+ inhibiting the redox reaction, which hampers the formation of oxOPD resulting in the diminution of IFE (inner filter effect) between CDs and oxOPD, then the fluorescence of CDs restores. ATP-induced dual-signal response of the CDs-OPD/Cu 2+ system makes ratiometric assay possible. The method exhibits high sensitivity toward ATP with a detection limit of 0.43 μmol L −1 . Furthermore, the semi-quantitative measurement of ATP is available by naked eyes through the color of fluorescence and solution. Finally, the developed method is employed for the quantification of ATP in practical samples with satisfactory results.
Shanliang Song,Chuanxi Wang,Yue Zhao,Tantan Hu,Xinxin Zhou,Tianxin Zhao,Minghui Yang,Quan Lin
A dual-emission ratiometric fluorescent sensing film for metal ion detection is designed. This dual-emission film is successfully prepared from chitosan, graphitic carbon nitride (g-C 3 N 4 ), and gold nanoclusters (Au NCs). Here, it is shown that the g-C 3 N 4 not only serves as the fluorescence emission source, but also enhances the mechanical and thermal stability of the film. Meanwhile, the Au NCs are adsorbed on the surface of chitosan film by the electrostatic interaction. The as-prepared dual-emission film can selectively detect Cu 2+ , leading to the quench of red fluorescence of Au NCs, whereas the blue fluorescence from g-C 3 N 4 persists. The ratio of the two fluorescence intensities depends on the Cu 2+ concentration and the fluorescence color changes from orange red to yellow, cyan, and finally to blue with increasing Cu 2+ concentration. Thus, the as-prepared dual-emission film can be worked as ratiometric sensing paper for Cu 2+ detection. Furthermore, the film shows high sensitivity and selectivity, with low limit of detection (LOD) (10 ppb). It is observed that this novel gold-cluster-based dual-emission ratiometric fluorescent sensing paper is an easy and convenient way for detecting metal ions. It is believed that this research work have created another avenue for the detection of metal ions in the environment.
危险品化学品经营许可证（带存储）