ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Rong Huang,Zhong Dong,Wanyi Zhang,Zhenggang Xue,Dongmei Deng,Wei Wang,Yuanyuan Li,Haibo He,Liqiang Luo
Background Organophosphorus pesticides contamination significantly threatens the ecological environment. Therefore, developing sensitive and efficient point-of-care detection methods is essential. Results In this work, Cu−N 4 single-atom nanozymes on nitrogen-doped carbon (Cu SAs/NC) with dual enzyme-mimicking activities (peroxidase-like and oxidase-like) were prepared for point-of-care detection of parathion using acetylcholinesterase inhibition-based colorimetric sensing mechanism. Cu SAs/NC shows high catalytic activity with high turnover number (K cat ) of ∼24.07 min −1 and K cat /K m of ∼1.35×10 5 M −1 min −1 . Experimental findings in combination with density functional theory simulations demonstrate that Cu centers in Cu SAs/NC play a pivotal role in intermediate binding optimization, which contributes to a decreased reaction energy barrier. Hence, a colorimetric sensing platform based on Cu SAs/NC was constructed for point-of-care detection of parathion, achieving a detection limit of 0.79 ng mL −1 . Significance This study provides a novel strategy for structuring single-atom nanozymes with enhanced enzyme-mimicking activity, showing great promise for advanced environmental protection.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2023
·
IF 4.1
Yifan Li,Shujun Ni,Yunping Wang,Ruichi Li,Hong Sun,Xuan Ye,Zhen Tian,Yalin Zhang,Jiyuan Liu
BACKGROUND Insecticide resistance continuously poses a threat to agricultural production. Chemosensory protein-mediated resistance is a new mechanism of insecticide resistance discovered in recent years. In-depth research on resistance mediated by chemosensory proteins (CSPs) provides new insight into aid insecticide resistance management. RESULTS Chemosensory protein 1 in Plutella xylostella ( PxCSP1 ) was overexpressed in the two indoxacarb-resistant field populations and PxCSP1 has a high affinity with indoxacarb. PxCSP1 was upregulated when exposed to indoxacarb and the knockdown of this gene elevated sensitivity to indoxacarb, which demonstrate that PxCSP1 is involved in the indoxacarb resistance. Considering that CSPs may confer resistance in insects via binding or sequestering, we explored the binding mechanism of indoxacarb in PxCSP1 -mediated resistance. Using molecular dynamics simulations and site-directed mutation, we found that indoxacarb forms a solid complex with PxCSP1 mainly through van der Waals interactions and electrostatic interactions. Between these, the electrostatic interaction provided by the Lys100 side chain in PxCSP1 , and especially the hydrogen bonding between the NZ atom and the O of the carbamoyl carbonyl group of indoxacarb, are the key factors for the high affinity of PxCSP1 to indoxacarb. CONCLUSIONS The overexpression of PxCPS1 and its high affinity to indoxacarb is partially responsible for indoxacarb resistance in P. xylostella . Modification of indoxacarb's carbamoyl group has the potential to alleviate indoxacarb resistance in P. xylostella . These findings will contribute to solving chemosensory protein-mediated indoxacarb resistance and provide a better understanding of the insecticide resistance mechanism. © 2023 Society of Chemical Industry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 16
·7722-7732
·
IF 3.3
Liran Tian,Xiangwei Song,Tianjiao Liu,Anfeng Li,Yang Ning,Xiuyi Hua,Deming Dong,Dapeng Liang
Based on the specific identification ability of aptamers towards a target, a combined UV-Visible with fluorescence detection method for acetamiprid was designed. In the absence of acetamiprid, AuNPs were uniformly distributed and had a good quenching ability for Rhodamine B's fluorescence at a higher NaCl concentration. In the presence of acetamiprid, a few AuNPs lost their protective effect and aggregated, meanwhile, the absorption peak of the UV-vis spectrum gradually redshifted from 520 nm to 680 nm. Additionally, the quenching effect of AuNPs on Rhodamine B was diminished when aggregation took place. Thus the presence of acetamiprid could be clearly determined using a UV-vis or fluorescence method. The combination of the two routes had a wider detection range and a detection limit as low as 0.0559 μM. This method was successfully used to determine acetamiprid in spiked lake water samples and would be a potential simple method for the determination of acetamiprid residues in natural water bodies.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Yue Tang,Xiangqiang Zhan,Jia Zheng,Zhengmin Xie,Shuangju Zhu,Yuangen Wu
The long-term and excessive use of organophosphorus pesticides (OPs) leads to hazardous residues in the environment, which threatens human health to a considerable extent. Colorimetric methods can quickly and readily identify pesticide residue , but they still have various challenges in accuracy and stability. Herein, a smartphone-assisted and non-enzymatic colorimetric biosensor was constructed for rapid monitoring of multiple OPs based on the enhanced effect of aptamer on the catalytic ability of octahedral Ag 2 O. It was demonstrated that the aptamer sequence could enhance the affinity of colloidal Ag 2 O to chromogenic substrates, and accelerate the generation of oxygen radicals such as superoxide radical (·O 2 − ) and singlet oxygen ( 1 O 2 ) from dissolved oxygen, thereby significantly increasing the oxidase activity of octahedral Ag 2 O. The color change of the solution can be easily converted to the corresponding RGB values by a smartphone for quantitative and rapid detection of multiple OPs. Hence, the smartphone-based and visual biosensor of multiple OPs was acquired with limit of detection of 10 μg L −1 , 28 μg L −1 and 40 μg L −1 for isocarbophos , profenofos and omethoate , respectively. The colorimetric biosensor also exhibited good recoveries in several environmental and biological samples, showing that it may have broad application prospects for detecting OPs residues.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2023
·
IF 4.3
Huihui Wang,Xiaojiao Zheng,Jiadong Wang,Shu Qin,Jindong Li,Yanli Qi,Xiaowen Wang,Xu Jing
The risk of the hazardous pesticide dichlorvos (DDVP) entering the human body through aquatic products is causing concern as global demand for aquatic products increases. Herein, a novel approach ground on switchable hydrophilicity solvent-based microextraction coupled with fluorescent detection of DDVP in aquatic products was established. Sodium nonanoate , a switchable hydrophilic solvent, was converted into nonanoic acid in-situ for use as an extractant under acidic conditions to extract DDVP from aquatic products. Thereafter, DDVP was hydrolyzed to dichloroacetaldehyde (DCA) by adjusting the extraction solution to an alkaline condition. The fluorescent substances were generated due to the condensation reaction of DCA and resorcinol which can achieve the quantification of DDVP. The assay had a linear relationship between 40 and 280 μg kg −1 , with a limit of detection of 2.4 μg kg −1 . The assay has been used to determine the residual content of DDVP in grass carp, sea cucumber, and abalone samples. The recoveries ranged from 93.8% to 106%. The strategy integrating microextraction and detection offers insight into monitoring pesticides in food products.
✓ 已验证
产品关联
DOI
高影响因子
PEST MANAGEMENT SCIENCE
·
2023
·
IF 4.1
Xiaomu Qiao,Tianhao Zhou,Jing Zhang,Lixin Zhang,Yaobin Lu,Jia Huang
BACKGROUND Insect RDL (resistant to dieldrin) receptors are essential pentameric ligand-gated chloride channels that mediate the neuroinhibitory effect of GABA, the chief inhibitory neurotransmitter in the central nervous system. These receptors serve as primary targets for various insecticides, including noncompetitive antagonists (NCAs) such as cyclodiene organochlorines and phenylpyrazoles, as well as allosteric modulators like meta-diamides and isoxazolines. This study focuses on a newly discovered A2′N mutation within the RDL receptors, identified in fipronil-resistant planthoppers. Despite in vitro electrophysiological studies have proposed its role in conferring target-site resistance, in vivo genetic functional validation of this mutation remains unexplored. RESULTS Our research employed toxicity bioassays, assessing various Rdl genotypes against a spectrum of insecticides, including fipronil, α-endosulfan, broflanilide, and isocycloseram. Results revealed distinct resistance profiles for A2′N and A2′S mutants, indicating different binding interactions of RDL receptors with NCAs. Significantly, the A2′N heterozygote showed substantial resistance to fipronil, despite its homozygous lethality. Molecular modeling and docking simulations further supported these findings, highlighting unique binding poses for fipronil and α-endosulfan. CONCLUSION This study confirmed that A2′N mutation of the RDL GABA receptor confer high resistance to fipronil in vivo . The observed resistance in A2′N mutants is likely attributable to a steric hindrance mechanism, wherein the introduction of larger side chains hampers fipronil binding, even in a heterozygous state. © 2023 Society of Chemical Industry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Shuqin Liu,Yiquan Huang,Yingming Duan,Zhangmin Xiang,Jian Liu,Xi Zhou,Zhiyong Chen
An in vivo solid-phase microextraction (SPME) fiber with high-coverage capture capacity of plant endogenous substances based on the porous covalent triazine framework (CTF) material was developed. The CTF fiber coupled with gas chromatographic quadrupole time-of-flight mass spectrometer (GC-QTOF-MS) analysis was used for monitoring untargeted endogenous metabolites in living Chinese cabbage plants ( Brassica campestris L. ssp. chinensis Makino (var. communis Tsen et Lee)). A total of 100 endogenous substances were identified, mainly including aldehydes, ketones, acids, alcohols, phenols, alkanes, alkenes, esters, isorhodanates, nitriles, as well as indole and its derivatives. Using the in vivo metabolites analysis method, Chinese cabbage plants at different growing stages demonstrated significantly statistical differences in plant metabolism. In addition, metabolic dysregulation of Chinese cabbage plants under fipronil pesticide contamination was observed. To summarize, the proposed approach provides a feasible method to capture metabolic information in living vegetables and for risk assessment of pesticide use during agricultural production.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Yuhang Liu,Yi Zhang,Pran Gopal Karmaker,Yuting Tang,Lilei Zhang,Feng Huo,Ya Wang,Xiupei Yang
The misuse of organic pollutants such as nitrofuran antibiotics (NFAs) and 2,6-dichloro-4-nitroaniline (DCN) has become a hot topic of global concern, and developing rapid, efficient, and accurate techniques for detecting NFAs and pesticides in water is a major challenge. Here, we designed a novel lead-based anion 2D metal–organic framework (MOF){[(CH3)2NH2]2[Pb(TCBPE)(H2O)2]}n (F3) with interlocking structures, in which TCBPE stands for 1,1,2,2-tetra(4-carboxylbiphenyl)ethylene. Powder X-ray diffraction and thermogravimetric analysis revealed that F3 has excellent chemical and solvent stability. It is worth noting that F3 has a grinding discoloration effect. The solvent-protected grinding approach achieved F3B with a high quantum yield (QY = 73.77%) and blue fluorescence, while the direct grinding method produced F3Y with a high quantum yield (QY = 37.27%) and yellow–green fluorescence. Importantly, F3B can detect NFAs in water rapidly and sensitively while remaining unaffected by other antibiotics. F3Y can identify DCN in water quickly and selectively while remaining unchanged by other pesticides. F3B demonstrated high selectivity and rapid response to NFAs at a limit of detection (LOD) as low as 0.26 μM, while F3Y indicated high selectivity and responded quickly to DCN in water at an LOD as low as 0.14 μM. The method was successfully applied to detect NFAs in actual water samples of the fish tanks and ponds as well as the pesticide DCN in soil samples. The recovery rates were 97.0–105.15% and 102.2–106.48%, and the relative standard deviations were 0.63–1.45% and 0.29–1.69%, respectively. In addition, F3B and F3Y can be made into fluorescent test papers for the visual detection of NFAs and DCN, respectively. Combined with experiments and density functional theory calculations, the mechanism of fluorescence quenching of MOFs by target analytes was also revealed.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Xiaoyu Su,Zhiyu Chen,Huan Wang,Lei Yuan,Kaiyi Zheng,Wen Zhang,Xiaobo Zou
A ratiometric electrochemical immunosensor, based on DNA tetrahedron nanostructure (DTNS), is introduced for vegetable phoxim determination. DTNS spontaneously adheres onto gold-nanoparticle-modified electrode and forms stable three-dimensional structure, providing plenty of binding sites to the built-in reference, methylene blue (MB). Monoclonal antibody (m-Ab) is vertically linked onto DTNS vertex, selectively responses antigenic phoxim, and promotes the target signal of I PHO . Thus, a ratiometric indicator, I PHO / I MB , is sensibly established with the target signal ( I PHO ) and the reference signal ( I MB ). Modifications, mechanisms and advances of the proposed method are subsequently examined with morphological methods and electrochemical experiments. This method brings considerable advances in analytical behaviors. The ratiometric signal presents better performance than solo system in repeatability and long-time stability. As-fabricated sensor presents wide dynamic range as 0.1∼30 μg/L, and limit of detection is well defined as 0.003 μg/L (S/N = 3). Finally, this method is verified with real-vegetable-sample analysis, certified HPLC and recovery test.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 189
·期 1
·1-10
·
IF 5.833
Liu Guangyan,Li Shiyu,Jiang Zejun,Li Jianping
A novel approach for trace detection of fipronil with a molecularly imprinted electrochemiluminescence sensor (MIECLS) is proposed. The sensitivity is significantly improved via signal amplification of the enzymatic reaction of horseradish peroxidase (HRP) released from encapsulated liposomes which linked onto the template molecules after rebinding. The molecularly imprinted polymer membrane was prepared through the electropolymerization of monomers with fipronil as a template. After the elution of the template molecules, the analyte fipronil was reabsorbed into the cavities. HRP-encapsulated liposomes were linked to the target molecules by light-triggered click reaction. The higher the concentration of the target was, the more HRP-encapsulated liposomes were present on the molecularly imprinted polymer (MIP) sensor. Then, HRP was liberated from liposomes, and the catalytic degradation of hydrogen peroxide (H 2 O 2 ) by HRP occurs, which changed the electrochemiluminescence intensity of luminol significantly. The change of the ∆ ECL intensity was linearly proportional to the logarithm of the fipronil concentration ranging from 1.00 × 10 −14 to 1.00 × 10 −9 mol/L, and the detection limit was 7.77 × 10 −16 mol/L. The recoveries obtained ranged from 95.7 to 105.8% with RSD < 5%. The sensitivity of the detection was significantly improved, and the analysis process was simplified in that the incubation step required in the conventional method was avoided. The sensor proposed provides a feasible platform for ultra-trace amount determination. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2023
·
IF 2
Liran Tian,Xiangwei Song,Tianjiao Liu,Anfeng Li,Yang Ning,Xiuyi Hua,Deming Dong,Zhijun Song,Dapeng Liang
Acetamiprid is mainly used to control pests. However, the residues of acetamiprid have resulted in significant negative effects on mammals. Many researchers are working on developing acetamiprid detection methods. In our study, four truncated aptamers were designed based on the secondary structure of the original aptamer. Molecular docking simulation of truncated aptamers confirmed that all of the truncated aptamers bind to acetamiprid, and their binding sites were identified. The affinity, sensitivity, and specificity of these truncated aptamers were measured by a spectrophotometric method with gold nanoparticles (AuNPs). Acetamiprid aptamers with 17-mer (Apt-J2) and 16-mer (Apt-J4) were successfully obtained, which show higher detection sensitivity and satisfactory specificity. Additionally, a spectrophotometric detection route superior to the original aptamer was explored, and the linear ranges were from 1 to 70 μM and 0.5 to 40 μM, respectively. The truncated aptamers were applied to the analysis of lake water, indicating that the aptamers have potential for practical analysis.
✓ 已验证
产品关联
DOI
高影响因子
Biology-Basel
·
2023
·Vol. 12
·期 9
·1214
·
IF 4.2
Ferry Saputra,Yu-Heng Lai,Marri Jmelou M. Roldan,Honeymae C. Alos,Charlaine A. Aventurado,Ross D. Vasquez,Chung-Der Hsiao
Simple SummaryIn recent years, the human population on Earth has increased, leading to a higher demand for food sources. Pesticides have emerged as a breakthrough to enhance crop yields. However, the excessive use of pesticides has raised concerns regarding their potential impact on aquatic animals. Thus, this study aims to assess the potential cardiovascular toxicity of various pesticides, with a specific focus on fenpropathrin. When compared to other pesticides, fenpropathrin causes cardiomegaly, an increased heart rate, a significant rise in blood flow velocity, and an elevated metabolism rate. Furthermore, it has been found to bind to several ion channels, inducing alterations in cardiovascular-related markers. These findings indicate that fenpropathrin induces cardiotoxicity in the zebrafish larvae model.AbstractFenpropathrin, a pyrethroid insecticide, has been widely used for many years in agricultural fields. It works by disturbing the voltage-gated sodium channel, leading to paralysis and the death of the target animal. While past studies have focused on neurodegeneration following fenpropathrin poisoning in humans, relatively few pieces of research have examined its effect on other peripheral organs. This study successfully investigated the potential toxicity of fenpropathrin on the cardiovascular system using zebrafish as an animal model. Zebrafish larvae exposed to varying doses of fenpropathrin underwent an evaluation of cardiac physiology by measuring the heart rate, stroke volume, cardiac output, and shortening fraction. The blood flow velocity and the dorsal aorta diameter were also measured to assess the impact of fenpropathrin exposure on the vascular system. Furthermore, molecular docking was performed to evaluate the pesticide binding affinity to various proteins associated with the cardiovascular system, revealing the potential mechanism of the fenpropathrin cardiotoxic effect. The findings demonstrated a significant dose-dependent increase in the heart rate stroke volume, cardiac output, shortening fraction, and ejection fraction of zebrafish larvae after 24 h of acute treatment with fenpropathrin. Additionally, zebrafish treated at a concentration of 1 ppm exhibited significantly larger blood vessels in diameter and an increased blood flow velocity compared to the control group. According to molecular docking, fenpropathrin showed a high affinity for various voltage-gated sodium channels like scn1lab, cacna1sb, and clcn3. Finally, from the results, we found that fenpropathrin caused cardiomegaly, which may have been induced by the voltage-gated sodium channel disruption. This study highlights the significant disruption of fenpropathrin in the cardiovascular system and emphasizes the need for further research on the health implications of this pesticide.Keywords:fenpropathrin;zebrafish;cardiovascular toxicity;pesticide
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）