PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2026
·
IF 4
Hongmei Wang,Bo Lei,Xinyu Bao,Xiaobin Gu,Jing Xia,Yong Huang,Huaqiao Tang,Fei Shi,Xing Huang,Bo liang,Jing Xu
Scabies (or Sarcoptic mange) is a highly contagious skin disease caused by the ectoparasite Sarcoptes scabiei. The resistance of S. scabiei to common acaricides like permethrin has been reported, yet their precise mechanisms remain elusive. Glutathione S-Transferase (GSTs) constitute an important class of detoxification enzymes. In this study, we found that the expression levels of SsGSTd3 and SsGSTd5 in S. scabiei were significantly up-regulated after exposure to LC 30 of permethrin, amitraz, and selamectin, suggesting that SsGSTd3 and SsGSTd5 are involved in the detoxification of these acaricides. Therefore, we used in vitro metabolic assay and bacterial survival assays to further investigate their specific detoxification mechanisms. Co-incubation with the acaricides reduced the 1-Chloro-2,4-dinitrobenzene (CDNB) activities of both recombinant proteins to varying degrees. HPLC analysis revealed that SsGSTd3 and SsGSTd5 metabolized 35.24% and 53.28% of permethrin, respectively, but did not metabolize amitraz. SsGSTd3 metabolized a small fraction of selamectin (3.25%). In addition, bacterial survival assays demonstrated that under oxidative stress induced by CHP and H 2 O 2 , the OD 600 values of E. coli overexpressing SsGSTd3 or SsGSTd5 were significantly higher than those of control group, indicating robust antioxidant activity for both proteins. In conclusion, our findings demonstrate that SsGSTd3 and SsGSTd5 facilitate permethrin detoxification via a dual mechanism—metabolic and alleviation of oxidative stress, and may represent an early warning sign of decreased susceptibility to amitraz and selamectin through their antioxidant functions.
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MICROCHEMICAL JOURNAL
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2026
·
IF 5.1
Yeru Liu,Mengxue Zhang,Xiaolei Zhao,Yifan Zhou,Hongna Yin,Li Zhou,Zhicheng Liu,Qiyong Cai
Glyphosate, a broad-spectrum herbicide extensively applied in agriculture, may bioaccumulate in humans via the food chain and pose toxicological risks. In this work, an integrated biosensing strategy for glyphosate is developed, integrating a self-developed Android application (APP) with alkaline phosphatase (ALP)-triggered silicon nanodot (Si NDs) fluorescence biosensing to achieve field-deployable quantification. In the sensing system, ascorbic acid (AA), produced by ALP-catalyzed substrate 2-phospho- l -ascorbic acid trisodium salt (AA2P), can reduce the silane precursor 3-aminopropyltrimethoxysilane (APTMS) at room temperature to nucleate and grow green fluorescent Si NDs with an emission wavelength of 505 nm. The fluorescence intensity is dynamically modulated by the inhibitory effect of glyphosate on ALP activity, enabling a quantitative relationship between pesticide concentration and signal output. The sensor displayed a robust linear response toward glyphosate across 0.005–100 μM, with detection limits of 2.4 nM (fluorimeter) and 18 nM (APP). Importantly, the custom-designed Android application integrates image acquisition, RGB signal extraction, and calibration modeling, transforming smartphone into portable analytical devices. This synergy between nanomaterial innovation and digital technology not only delivered high recovery rates (80–109.5%) in spiked real samples but also demonstrated excellent reproducibility (RSD < 2.9%) and selectivity, underscoring its promise as a next-generation strategy for on-site glyphosate monitoring.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Yuhan Zhao,Xiaojie Zhou,Mei Li,Xinghui Qiu
Aedes albopictus is a primary vector of arboviral diseases such as dengue fever, chikungunya fever, and Zika. The rapid development of insecticide resistance is becoming a major challenge to sustainability of insecticide-based interventions for the control of Ae. albopictus borne arboviral diseases. Understanding the molecular basis of resistance is useful for developing effective insecticide-based control action. Our recent study showed that overexpression of the AalbCYP9J26 gene was associated with insecticide resistance in field Ae. albopictus populations in Beijing, but its contribution to insecticide metabolism and resistance remains unknown. In this study, the metabolic capacity of AalbCYP9J26 towards six insecticides was examined using an enzyme system reconstituted with Escherichia coli -produced AalbCYP9J26 protein and its redox partners (AalbCPR and Aalbb5). The results showed that AalbCYP9J26 was able to metabolize temephos, malathion, propoxur, permethrin, and deltamethrin, while no significant activity against bendiocarb was observed. Moreover, the Drosophila GAL4/UAS transgenic system confirmed that enhanced expression of AalbCYP9J26 conferred increased tolerance to malathion, permethrin, deltamethrin, and propoxur. This study demonstrates that AalbCYP9J26 plays a critical role in insecticide metabolism and resistance, suggesting AalbCYP9J26 may serve as a valuable biomarker for monitoring insecticide resistance and a target for new insecticidal agents.
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JOURNAL OF CHROMATOGRAPHY A
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2026
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IF 4
Huiru Xu,Yuqing Zong,Kehan Yin,Rimao Hua,Xiangwei Wu,Jiaying Xue
Water resources are increasingly contaminated by industrial and agricultural pollutants, with pesticide residues posing a persistent challenge. Most existing methods were designed for a single purpose of either measuring or eliminating water pollutants, while integrated strategies containing both functions remain scarce. Here we reported a novel semi-automatic silicone rubber (SR) based multi-vial microextraction technique for the simultaneous determination and removal of fifteen pesticides from aqueous matrices. An integrated adsorption-desorption platform enabled effective in-situ extraction of the target pesticides via hydrophobic effects. Key parameters including ultrasonication extraction for 45 min and two sequential 5-min desorption with 5 mL of hexane/ethyl acetate (1:1, v/v) were employed. The quantitative analysis was validated in real waters of the Nanfei River, Chao Lake, and tap water with good recoveries (72.8–112.6 %) and relative standard deviations (0.1–7.5 %), superior limits of detection (0.0020–0.20 μg/L) and quantification (0.006 to 0.50 µg/L), and free matrix effects (87.5–102.1 %) by gas chromatography equipped with electron capture detector. Focusing on its selectivity and recyclability, the proposed approach was further applied to purifying green tea and chrysanthmum tea infusions, achieving over 90 % removal efficiency for most pesticides. These results demonstrated that the semi-automatic SR-based multi-vial microextraction is a robust and effective method for both quantifying and eliminating target pesticides from waters and tea infusions. The high throughput and low cost encourage its potential for customized commercial application in monitoring and remediating emerging pesticides in environment and food fields.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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2022
·Vol. 29
·期 60
·91155-91164
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IF 5.19
Xiang Xiangmei,Dong Kangyu,Li Zhanjun
Microplastics (MPs) have aroused tremendous attentions because of their ability in accumulating contaminants from adjacent environment. However, their capability of concentrating hydrophobic organic contaminants might be influenced by huge amounts of coexisting natural particles (NaPs). Yet, our knowledge about the relative concentrating capability of MPs versus NaPs is still rare. Herein, the distribution behavior of several hydrophobic pollutants was studied in simplified MPs/NaPs/pollutant/water quaternary systems. We found that 1-(4-(phenylzao)phenyl)azo-2-naphthol (Sudan III) had similar distribution behavior with many hydrophobic pollutants and thus was used as a model pollutant. Polyethylene (PE) film debris derived from disposable plastic wrap was the most efficient microplastic which concentrated tens of times Sudan III than coexisting NaPs including bentonite, kaolin, sawdust, and diatomite. An enrichment coefficient of over 1000 times was observed in the case of PE film debris versus quartz sand. The concentrating capability of PE film MPs was found approximately equal to biochar particles. MPs seem to have limited contribution on the long distance transportation of hydrophobic organic pollutants. However, notably, over 70% of the adsorbed Sudan III could be released back into water within 0.5 h in the presence of 0.5% sodium oleate, which is a common amphipathic matter forming micelles in digestive fluid. Microplastics might play an important vector-like role in increasing the biological accumulation of hydrophobic pollutants via intestine digestive absorption. Due to much bigger quantity of NaPs, the relative concentrating effect of MPs to hydrophobic organic pollutants over NaPs is possibly a more reasonable reference when considering their potential ecological risk.
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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2021
·
IF 3.963
Kai Tian,Jing Feng,Jiang Zhu,Jiagao Cheng,Mei Li,Xinghui Qiu
The cotton bollworm , Helicoverpa armigera , is a polyphagous pest threatening many economically important crops worldwide. Until recently, synthetic pyrethroids remain in wide use for controlling pest insects including the cotton bollworm. Understanding the metabolic mechanism of pyrethroids in a given pest can provide significant implication for a smart choice of insecticides, and such information is useful for the development of novel selective and safe insecticides. In this study, we used complexes of recombinant H. armigera cytochrome P450 CYP9A and NADPH-dependent cytochrome P450 reductase to investigate the capacity of three CYP9A paralogs in the transformation of seven structurally different pyrethroids by metabolism assays. The results showed that the three paralogous CYP9As were able to metabolize multiple pyrethroids. Interestingly, all the three CYP9As transformed pyrethrin-resembling pyrethroids (e.g. bioallethrin) more efficiently than the heavily modified ones (e.g. bifenthrin). These findings suggest that herbivorous insects can cope with synthetic insecticides using their physiological systems that initially evolved to survive exposure to the defensive chemicals in their host plants, adding support to the pre-adaptation hypothesis.
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ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
·
2021
·Vol. 108
·期 2
·e21835
·
IF 1.698
Mao-Ye Li,Yun Wang,Xiao Lei,Chuan-Tao Xu,Dong-Dong Wang,Su Liu,Shi-Guang Li
The green peach aphid, Myzus persicae (Sulzer), is a serious agricultural pest with a worldwide distribution. Catalase (CAT), which is encoded by the catalase ( Cat ) gene, is an extremely important antioxidant enzyme that plays a pivotal role in protecting cells against the toxic effects of hydrogen peroxide. The Cat gene has not been characterized in M. persicae ; therefore, this study describes the identification of the Cat ( MpCat ) gene from M. persicae . MpCat contains an open reading frame of 1515 bp and encodes a MpCAT protein consisting of 504 amino-acid residues. MpCAT possesses features typical of other insect catalases, including 7 conserved amino acids involved in binding heme and 15 involved in binding nicotinamide adenine dinucleotide phosphate. Phylogenetic analysis showed that MpCAT was closely related to orthologs from other aphid species. MpCat consisted of nine exons and eight introns, and the number and insertion sites of introns are consistent with those of Cat genes from Acyrthosiphon pisum (Harris) and Aphis gossypii Glover. The mRNA transcripts of MpCat were detected at all tested developmental stages, with the highest mRNA level in alate adults. The expression of MpCat was significantly upregulated when M. persicae was exposed to low and high temperatures, ultraviolet radiation, Beauveria bassiana , and permethrin. The transcription of MpCat and the activity of catalase were suppressed by RNA interference, and knockdown of MpCat significantly reduced the survival rate in M. persicae under heat stress. The results provide valuable information for further study on the physiological functions of MpCat . Research Highlights A catalase gene ( MpCat ) was characterized in Myzus persicae . Transcription of MpCat was responsive to various environmental stresses. Knockdown of MpCat increased mortality in M. persicae under heat stress.
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TALANTA
·
2021
·
IF 6.057
Yuheng Guo,Wenmin Zhang,Hui Chen,Qingqing Ding,Qingqing Li,Lan Zhang
The tailor-prepare solid phase microextraction (SPME) coatings with stable and excellent properties to effectively extract analytes from sample matrix still remains a challenge. Herein, a nitrogen doped graphitic carbon networks (NG-CNTW) coated fiber was fabricated by direct carbonization of nanosized ZIF-67 crystals (nano-ZIF-67) that grown on stainless steel wire. The NG-CNTW coated fiber coupled with gas chromatography-tandem mass spectrometry (GC-MS/MS) was applied for enrichment and determination of pyrethroids . The NG-CNTW coating exhibited high surface area and hierarchical porous structures that facilitate diffusion and accessibility of target molecules. Simultaneously, the nitrogen doped and highly graphitic structures endow the coating with high adsorption affinity for aromatic compounds . Under optimum conditions, the SPME-GC-MS/MS method presented wide range of linearity performance (0.08–200.0 ng g −1 ), low limits of detection (0.02–0.5 ng g −1 ) and good repeatability (RSD < 9.6%) for 8 kinds of pyrethroids . Furthermore, the proposed method was successfully applied in the determination of pyrethroids in grape and cauliflower samples, as the results were in the range of 3.16–15.06 ng g −1 and 2.08–9.29 ng g −1 , respectively. This work not only provides a new method by fabricating carbon nanomaterial coatings in situ derived from MOFs , but also shows great potential of MOFs derivative materials in environmental analysis field.
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INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
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2021
·
IF 2.826
Songqing Li,Wentao Han,Wanning Zhao,Youqing Luo
In recent years, more and more interest has been focused on the on-site sample preparation. Dispersive liquid–liquid microextractions without centrifugation or toxic organic solvent were developed and proved suitable for on-site extraction. In this study, a flexible dispersive liquid–liquid microextraction was developed for on-site water sample pre-concentration. A device containing a phase separation filter was prepared. By means of the phase separation filter packed with hydrophobic nonwoven polypropylene fibre, extraction phase and aqueous phase of the emulsified system were separated by manual filtration, which avoided the electric devices usually required in dispersive liquid–liquid microextractions. Combining the device with ionic liquid-based in situ dispersive liquid–liquid microextraction, a totally electricity-free procedure was developed. During the procedure, the extraction phase 1-hexyl-3-methylimidazolium bis[(trifluoromethane)sulfonyl]imide was formed through an ion exchange reaction, and then separated with water by filtration. After filtration, the extraction phase was eluted and submitted to high-performance liquid chromatography-variable wavelength detector for analysis. Parameters affecting the extraction performance were investigated and optimised. The optimised procedure achieved extraction recoveries of 75–89%. The intra-device, inter-device, intra-day and inter-day relative standard deviations were less than 4.6%. Good linearity (r2 > 0.9992) was obtained over the calibration range from 2 to 1000 µg L−1. The limits of detection of the method were in the range of 0.41–0.65 µg L−1. Because electric devices and organic dispersants are not required, the present approach is proved green, fast, and extremely flexible for on-site preparation of water sample. The proposed device is also expected to make other dispersive liquid–liquid microextractions transform into the on-site procedure.
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Analytical Methods
·
2020
·Vol. 13
·期 5
·672-684
·
IF 2.596
Xiao-Chun Ma,Zong-Qin Ma,Meng-Xin Zhao,Ying-Hui Wang,Yuan Peng,Xin Guo,Fang-Huan Wang,Zhe Meng,Hao-Bo Zheng
A novel magnetic molybdenum disulfide@graphene (Fe3O4/MoS2@G) nanocomposite with amphiphilic properties was prepared via a co-mixing solvothermal method. To demonstrate the feasibility of Fe3O4/MoS2@G as a sorbent during sample preparation, it was employed for the magnetic solid phase extraction (MSPE) of ten pyrethroids, three triazoles and two acaricide pyridaben and picoxystrobin in an emulsified aqueous solution. Dichloromethane was used as the extractant to form an emulsified aqueous solution. Subsequently, the Fe3O4/MoS2@G sorbent with amphiphilic properties was used to retrieve 15 wide polarity insecticides from dichloromethane via MSPE. The proposed method has the advantage of being applicable to different polar pesticides, strengthening the capacity of enrichment and purification of target analytes. The π–π interaction between the hydrophilic and hydrophobic moieties of Fe3O4/MoS2@G and the aromatic rings of target analytes were responsible for the efficient sorption. Thus, a reliable, convenient, and efficient method for the analysis of 15 insecticides with wide polarity in wolfberry samples was established by coupling Fe3O4/MoS2@G nanocomposite MSPE with gas chromatography-mass spectrometry (GC-MS) analysis. The obtained linearity of this method was in the range from 1 to 5000 ng mL−1 for 15 analytes, with determination coefficients (R2) ≥0.9907. The limit of detection (LOD) for 15 insecticides was in the range from 0.1 to 5.0 ng g−1. The recoveries of 15 insecticides from spiked wolfberry samples were in the range from 71.41% to 110.53%, and RSD was less than 14.8%.
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Qiuyan Zhu,Yang Yang,Zhiting Lao,Yingying Zhong,Kun Zhang,Suqing Zhao
Photochemical methods attracted much research interests for their high-efficiency and low secondary pollution. Decomposition of synthetic pyrethroids , the fourth major group of insecticides in use worldwide, was also of great significance due to their possible environmental risks. The photodegradation of deltamethrin , permethrin and dihaloacetylated heterocyclic pyrethroids in methanol/acetone = 9/1 (by volume) by a 400 W mercury lamp was examined. The t 1/2 of tested pyrethroids was less than 25 min, except for cis -permethrin with a t 1/2 of up to 50 min. The trans -isomer of permethrin and compound DCA-01 with a smaller t 1/2 might be more susceptible to degradation than their cis -isomer. Besides, the photodegradation of pyrethroids was divided into twelve pathways including isomerization , ester hydrolysis, ester bond cleavage, C O bond cleavage, 3,3-dimethylacrylate formation, double bond break, C1-C3 bond cleavage in cyclopropyl, reductive dehalogenation, decarboxylation , nucleophilic reagents attack on lone pair electrons on oxygen atoms in the phenyl ether, cyano hydrolysis, and halogenated hydrocarbon hydrolysis. The ECOSAR program displayed that pyrethroids and most of their photodegradation products were toxic to fish, daphnid, green algae . Particularly, some photodegradation products were more harmful to aquatic organisms than their parents.
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PEST MANAGEMENT SCIENCE
·
2020
·Vol. 76
·期 12
·4210-4221
·
IF 3.75
Qiuyan Zhu,Yang Yang,Zhiting Lao,Yingying Zhong,Kun Zhang,Suqing Zhao
BACKGROUND Pyrethroids, a class of insecticides, that act on the nervous system of insects. Frequent consumption of foods with pyrethroid residues increase the risk of developmental and neurological diseases in humans. Assessing the toxicity of novel synthetic pyrethroids to mammals is also critical to the development of agrochemicals. RESULTS Using mice as models, the acute and chronic toxicity of deltamethrin, permethrin, dihaloacetylated pyrethroids to mammals was researched by gavage administration. Acute toxicity assessment displayed that the median lethal dose (LD 50 ) of deltamethrin, permethrin and dihaloacetylated heterocyclic pyrethroids DCA-O, DCA-01, and DCA-11 tested were greater than 500 mg/kg of weight. Furthermore, chronic toxicity assessment demonstrated that deltamethrin, permethrin caused epidermal damage near the genitals, while dihaloacetylated heterocyclic pyrethroids DCA-O, DCA-01, and DCA-11 showed no relevant symptoms. However, both the acute and chronic toxicity assessment suggested that pyrethroids exposure induced mice loss weight. Additionally, the elevated plus maze (EPM) test showed that pyrethroids caused anxiety-like behaviors and no motor defects in Kunming mice. Beside, during the sucrose preference test (SPT), 60-day pyrethroids exposure increased excitatory behaviors in mice. However, the neurochemical studies displayed that pyrethroids exposure increased the total amount of glutamate (Gln), glutamine (Glu) and γ -aminobutyric acid (GABA) in the mice's blood. CONCLUSION Pyrethroids exposure induced weight loss in mice, although the acute oral toxicity of deltamethrin, permethrin and dihaloacetylated heterocyclic pyrethroids DCA-O, DCA-01, and DCA-11 was low. However, regarding chronic toxicity, deltamethrin, permethrin and dihaloacetylated heterocyclic pyrethroids DCA-O, DCA-01, and DCA-11 induced anxiety-like behaviors, excitatory behaviors, Gln-Glu-GABA circulatory dysfunction in blood. Particularly, deltamethrin, though permethrin also had reproductive toxicity. © 2020 Society of Chemical Industry
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