JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2026
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IF 9.6
Liming Wang,Yupeng Liu,Gonghua Hu,Liang Xiong,Yuanfu Ding,Bingzhe Wang,Xiaofeng Lin,Hanqiang Zhang,Qitong Huang,Songnan Qu,Licen Li
Nicotinamide-derived carbon dots (NAM-CDs) display amplified Ki67 binding, conferring potent tumor targeting. • Optimized solvothermal synthesis at 180 °C boosts red fluorescence and photothermal conversion. • Excellent biocompatibility and tight Ki67 binding enable selective nuclear retention of NAM-CDs in cancer cells. • In vivo 660 nm irradiation of NAM-CDs achieves complete tumor regression without recurrence over 30 days. The lack of tumor-specific targeting remains a major bottleneck in photothermal therapy. Here, we exploit the natural affinity of nicotinamide (NAM) for Ki67, a tumor proliferation marker, to design a targeted photothermal nanomaterial. NAM-derived carbon dots (NAM-CDs) were synthesized via a one-step solvothermal method, in which the carbon core serves as a scaffold for the multivalent presentation of NAM moieties. Dynamic molecular docking simulations revealed that NAM-CDs exhibited a markedly enhanced binding affinity toward Ki67, with binding energy of −7.7 kcal/molcompared with −3.8 kcal/mol for free NAM. These results indicate that the multivalent display of NAM not only preserves but also amplifies its intrinsic Ki67-targeting capability. To further optimize the photophysical properties of NAM-CDs, the synthesis temperature was systematically tuned. At 180 °C, the resulting NAM-CDs developed a pyridine-rich surface characterized by maximized pyrrolic-N content and enhanced amide bond formation, facilitating efficient energy transfer from the carbon core to surface states. This optimized electronic structure synergistically enhanced both red fluorescence emission and photothermal conversion efficiency. The pyridine-rich NAM-CDs exhibited excellent biocompatibility, specific nuclear retention through Ki67-mediated interactions in cancer cells, and potent photothermal tumor ablation upon 660 nm laser irradiation in vivo, achieving complete tumor regression without recurrence over a 30-day observation period. By harnessing the inherent Ki67 affinity of NAM, this work provides a facile strategy for imparting tumor-targeting capability to photothermal agents, opening a new avenue for proliferation-marker-directed cancer therapy. In this work, we developed nicotinamide-derived carbon dots that exploit natural Ki67 affinity to amplify binding, with a pyridine-N-rich surface boosting fluorescence and photothermal conversion. They achieve selective nuclear retention in cancer cells and complete tumor regression without recurrence. Download: Download high-res image (119KB) Download: Download full-size image
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Journal of Pharmaceutical Innovation
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2026
·Vol. 21
·期 4
·340
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IF 2.6
Hou Bo,Ma Dewei,Yang Keke,Peng Mingwei,Liu Qinglong,Heng Ziwen,Chen Siyuan,Liu Wei
Purpose Retinol (RET) and niacinamide (NA) are widely used in anti-aging and skin-brightening skincare products. However, RET is unstable under light and oxygen, while NA exhibits poor skin permeation. This study aimed to develop a liposomal co-delivery system (RN-Lip) to overcome these limitations and enhance the synergistic efficacy of the two compounds. Method RN-Lip was prepared by lipid film hydration, followed by extrusion to decrease particle size. The liposomal formulation was optimized, and its stability was evaluated under various conditions. Additionally, in vitro transdermal delivery, cellular uptake, cytotoxicity, and cellular efficacy evaluations were performed. Results The optimized RN-Lip exhibited a particle size of 147.03 ± 3.76 nm. The encapsulation efficiencies (EE) for NA and RET were 19.47% and 41.67%, respectively. Liposomal encapsulation improved the stability of RET, with a 7-fold higher retention rate than free RET after 30 days of storage at room temperature (under light protection). Compared to free NA and RET, liposomal encapsulation increased the skin retention of NA and RET by 3.8-fold and 1.5-fold, respectively, and further enhanced cellular uptake. Furthermore, RN-Lip reduced melanin content and tyrosinase activity in B16F10 cells compared to the free combination of RET and NA, promoted collagen I synthesis in BJ cells, and protected HaCaT cells against H₂O₂-induced oxidative damage. Conclusion RN-Lip successfully co-delivered RET and NA, overcoming their individual limitations and boosting their skincare functionalities. Thus, this system holds significant potential for advanced anti-aging and skin-brightening applications.
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CHEMICAL ENGINEERING JOURNAL
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2026
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IF 12.5
Pan Huang,Xiao-Bo Zhang,Jia-Qi Li,Guan-Wen He,Ying Chen,Ling-Yun Yang,Zai-Long Chi
LF-EVLPs treatment ameliorates retinal injury in mice. • LF-EVLPs mitigates oxidative stress, inflammation and complement factors in retinal injury. • LF-EVLPs exert retinal protective effects through the synergistic actions of multiple active ingredients. Oxidative stress and inflammation are major contributors to the development of age-related macular degeneration (AMD), which is one of the retinal degeneration (RD) diseases that are characterized by progressive damage to photoreceptors and retinal pigment epithelium (RPE) cells. This condition can ultimately lead to blindness. Lycii Fructus can clear liver heat, improve vision, and protect the optic nerve. Thus, the aim of this study was to investigate whether nanovesicles derived from Lycii Fructus (LF) can alleviate retinal damage. LF extracellular vesicle-like particles (EVLPs) were isolated from fresh Lycii Fructus . Intravitreal injection of LF-EVLPs resulted in significant retinal protective effects, such as the preservation of RPE cell morphology and junctional integrity, enhanced photoreceptor survival, improved retinal thickness, and restored visual function, in sodium iodate-induced retinal injury mice. Moreover, LF-EVLPs reduced retinal ROS levels and suppressed glial cell activation. Further experiments demonstrated that LF-EVLPs exert neuroprotective effects by modulating the NF-κB signalling pathway and suppressing the aberrant activation of proinflammatory cytokines ( IL-1β and TNF-α ) and complement factors ( C3, C4b, and C5 ). Through an integrated analysis of LF-EVLPs components and the retinal proteome, we identified 12 components and 17 target genes. We confirmed that the active ingredients caffeic acid phenethyl ester (CAPE), a derivative of caffeic acid, and nicotinamide (NAM) exhibit neuroprotective effects in sodium iodate-induced retinal injury mice. Our research provides new insight into neuroretinal protection, for which plant-derived EVLPs may be a promising therapeutic approach. LF-EVLPs preserve the structural and functional integrity of the retina by delivering bioactive constituents and attenuating oxidative stress, modulating the NF-κB pathway, suppressing inflammatory responses, and regulating the complement system after retinal injury. Download: Download high-res image (133KB) Download: Download full-size image
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CHEMISTRY-A EUROPEAN JOURNAL
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2025
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IF 3.7
Mi Wang,Tianqi Liu,Hao Wang,Zhenyuan Wang,Jiaheng Zhang
Graphical This study presents a sustainable supramolecular system composed of naturally derived ingredients: PE, AHP8, and a bioactive ionic liquid from betaine and MA, which offers multiple anti-aging benefits while promoting skin permeability. The growing demand for safe and sustainable cosmetics has led to a marked inclination toward natural extracts and peptides as raw materials. In this study, we developed a sustainable system with multiple antiaging effects by combining peony extract (PE) and acetyl hexapeptide-8 (AHP8) to simultaneously treat static and dynamic wrinkles, as well as skin dullness. As the poor skin permeability of AHP8 limited its efficacy, we used a bioactive ionic liquid derived from betaine and malic acid (MA) ([Bet][MA]) as a permeation enhancer. In vitro and in vivo zebrafish experiments indicated that [Bet][MA] not only significantly enhances transdermal and cellular penetration, but also exerts synergistic anti-aging effects. While being biosafe, [Bet][MA] significantly increased the transdermal permeation, skin retention, and cellular uptake of AHP8. Theoretical calculations revealed that [Bet][MA] improved the anti-wrinkle and whitening efficacies of AHP8 and PE through supramolecular interactions; it modulated their electron cloud distribution and molecular polarity and thus intensified their affinity to target proteins. The supramolecular PE/AHP8/[Bet][MA] system demonstrated excellent radical- and reactive oxygen species-scavenging capability, collagen production, and tissue repair capability while concurrently inhibiting tyrosinase activity, motion signaling, and inflammation. The study findings pave the way for sustainable and multifunctional skincare solutions.
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CURRENT APPLIED PHYSICS
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2025
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IF 3.1
Mengyu Guan,Ranran Bu,Haizhen Song,Changyuan Xu,Yichao Wang,Yan Zhang,Hongming Yin
Mn 2+ -based organic-inorganic hybrid metal halides were synthesized via evaporation crystallization using benzamide (BZM) and nicotinamide (NCM) as organic ligands. The DSC curve shows that the Mn 2+ -based organic-inorganic hybrid metal halides contain two crystal forms of BZM. Steady-state photoluminescence (PL) spectra reveal that the PL emission of Mn 2+ shifts from red to green as the crystal form of BZM changes. Density functional theory (DFT) calculation suggests that there is a certain rule between the transformation of BZM crystal form and the change of Mn 2+ luminescence characteristics.
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Chemical Science
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2025
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IF 7.4
Yiming Liu,Shanshan Du,Xiangxiang Zhang,Chao Li,Shubin Li,Wenxia Xu,Jingjing Zhao,Wei Mu,Xiaojun Han
Reduced nicotinamide adenine dinucleotide (NADH) salvage pathway reconstitution is a crucial step toward autonomous artificial cells. In living systems, D-ribose is a fundamental precursor intricately involved in the synthesis of nucleotides, nucleic acids, and critical metabolic pathways. An NADH synthesis pathway in artificial cells starting from D-ribose was constructed with five-enzyme cascade containing ribokinase, ribose-phosphate pyrophosphokinase, nicotinamide phosphoribosyltransferase, nicotinamide mononucleotide adenylyltransferase, and formate dehydrogenase (RK, RPPK, NAMPT, NMNAT, and FDH), which efficiently converted 10 mM D-ribose into 415 μM NADH within 80 minutes under optimized conditions. The produced NADH was further used to drive the amino acid metabolism, i.e., to convert NH4+ and α-ketoglutarate to glutamate by introducing additional glutamate dehydrogenase (GDH) inside artificial cells. The successful reconstitution of NADH synthesis pathway lays the foundation for fabricate artificial cells with complicated metabolic net works.
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Pharmaceuticals
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2026
·Vol. 19
·期 3
·469
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IF 5.7
Lili Li,Xiaoya Zhang,Guangyuan Tang,Jianxin Wu,Qing Huang
Background: Glabridin, a natural compound derived fromGlycyrrhiza glabraL., possesses skin-lightening effects. This study aims to further elucidate the depigmentation mechanism of glabridin by investigating its effects on melanogenesis and melanin transfer.Methods: We initially confirmed the anti-melanogenic effects of glabridin in MNT-1 human melanoma cells. Then, we investigated the mechanism of its anti-melanogenic effect by evaluating the protein expression of β-catenin and MITF via Western blot. To investigate melanin transfer, we compared glabridin’s efficacy with that of niacinamide, a recognized inhibitor of melanosome transfer and employed two complementary experimental models: (1) α-melanocyte-stimulating hormone (α-MSH)-stimulated MNT-1 cells to analyze dendrite formation, and (2) a UVB-irradiated co-culture system of MNT-1 cells and HaCaT keratinocytes to evaluate melanin transfer.Results: By measuring glabridin’s effects on melanin content, tyrosinase activity, and melanogenesis-related protein expression confirmed its inhibition of melanin synthesis. Further investigation demonstrated that glabridin suppresses melanogenesis by downregulating β-catenin and MITF, indicating inhibition of the Wnt/β-catenin pathway. Furthermore, in α-MSH-treated MNT-1 cells, both glabridin and niacinamide were found to suppress dendrite formation and elongation. In a UVB-exposed co-culture system, both glabridin and niacinamide inhibited melanin transfer to keratinocytes. Mechanistically, these effects were linked to the regulation of Rho GTPases (Rac1, RhoA, Cdc42) and suppression of F-actin reorganization.Conclusions: This study provides, for the first time, evidence that the skin-lightening effect of glabridin involves two complementary mechanisms: inhibition of melanogenesis through suppression of the Wnt/β-catenin pathway, and attenuation of both dendricity and melanin transfer via the influence of Rho family GTPases expression.
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Cell Death Discovery
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2026
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IF 10.4
Meng Li,You Jiawen,Zhang Zhongyin,Jiang Yucheng,Liu Yulan,Zhou Mingliang,Ma Junqing,Jiang Xinquan
Cleft palate, a prevalent congenital disorder, arises from dysregulated embryonic palatal fusion, but the posttranslational modifications (PTMs) driving this process remain poorly understood. Here, we report that lysine acetylation is a critical MSX1 proteostasis switch that governs embryonic palatal mesenchymal (EPM) cell survival. We demonstrate in vitro and in vivo that MSX1 protein stability regulation by deacetylase SIRT1-catalyzed acetylation underlies EPM apoptosis and palatal fusion. In atRA-induced cleft palate models, SIRT1 suppression drives MSX1 hyperacetylation, accelerating proteasomal degradation and culminating in EPM apoptosis. Strikingly, transcriptomic profiling revealed the exclusive proteostatic role of acetylation, indicating that MSX1’s structural stability differs from its transcriptional activity—a paradigm distinct from that of classic PTM mechanisms during development. Lentivirus-mediated delivery of the deacetylase SIRT1 or the deacetylation mimic MSX1 K139R significantly reduced cleft severity, indicating its preventive and therapeutic potential in humans. Our work establishes the MSX1 acetylation as both a pathogenic driver and a druggable target in cleft palate, redefining PTM regulation as a central etiological factor in genetic disorders.
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Brazilian Journal of Pharmaceutical Sciences
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2023
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IF 1.3
Yang Xintong,Chen Yang,Qiao Ning
In this study, we investigated the effects of polymers on the pharmaceutical cocrystal formation process. Ibuprofen (IBU) was selected as the active pharmaceutical ingredient (API), nicotinamide (NIC) and saccharin (SAC) as the cocrystal coformer (CCF), ethanol/water as the solvent, polyvinylpyrrolidone (PVP) and poly (ethylene glycol) (PEG) as the representative polymers. We prepared IBU-NIC and IBU-SAC cocrystals in ethanol-water cosolvent in the absence or presence of polymers. Cocrystal screening products were characterized by FTIR, DSC, PXRD, and HPLC. The results showed that the mixture of IBU and IBU-NIC cocrystal can be prepared in ethanol-water cosolvent without polymers. The addition of PVP facilitates the formation of pure IBU-NIC cocrystal; however, no cocrystal was formed in PEG solutions. SAC could not cocrystallize with IBU in the ethanol-water solvent in the absence of polymers. Neither PVP nor PEG could facilitate the formation of the IBU-SAC cocrystal.
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JOURNAL OF CHROMATOGRAPHY A
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2022
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IF 4.601
Qiurong Luo,Meijun Wan,Jingqiu Zhou,Xuemei Dai,Honglin Yang,Fengjiao Zu,Yunchao Zheng,Lujun Wang
The bovine serum albumin (BSA) was firstly bonded on the surface of graphene quantum dots (GQDs) functionalized silica as the stationary phase named as BSA@GQDs@SiO 2 which can perform hydrophilic interaction between the stationary phase and analytes. Characteristic methods including Fourier transform infrared spectroscopy (FT-IR), elemental analysis (EA), thermogravimetric analysis (TGA) and scanning electron microscope (SEM) were applied to estimate the chemical bonding results and morphological features of the prepared materials. In the chromatographic evaluation part, the BSA@GQDs@SiO 2 column showed excellent separation efficiency for the analysis of hydrophilic compounds involving nucleosides and bases, acids, phenols, quinolones, vitamins and alkaloids, which proved the prepared column can exhibit hydrophilic interaction mode with other interactions including hydrogen bonding and electrostatic interaction. What's more, essential experimental approaches were designed to evaluate the retention properties of the prepared column via comparing with a commercial amino column and a prepared GQDs@SiO 2 column. Results indicated the prepared column showed excellent separation ability for different kinds of hydrophilic compounds than commercial and single hydrophilic columns. The study showed that BSA@GQDs@SiO 2 stationary phase with double hydrophilic materials will be a potential chromatographic material for analyzing various hydrophilic analytes.
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Arabian Journal of Chemistry
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2022
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IF 6.212
Chun-Yan Zhang,Wei-Yi Zhang,Guo-Ying Chen,Tong-Qing Chai,Hao Zhang,Yi Xu,Feng-Qing Yang
Small-molecule enzyme mimics as biocatalysts have been extensively applied in diverse colorimetric sensors fabrication. However, excavating potential organic enzyme mimics with high catalytic activity still remains challenging. In this study, the peroxidase mimicking activity of nicotinic acid (VB3) was demonstrated for the first time through chromogenic substrate 3, 3′, 5, 5′-tetramethylbenzidine (TMB) at the existence of hydrogen peroxide (H 2 O 2 ). The catalytic activity of VB3 kept more than 80% of its optimum activity in a broad pH range of 3.0–9.0. In addition, the kinetic parameter (Michaelis constant, K m = 0.037 mM) of VB3 catalysis to H 2 O 2 is smaller than natural horseradish peroxidase (HRP) and previously reported peroxidase mimics. The catalytic mechanism of VB3 is mainly attributed to the active species of hydroxyl radical ( OH) and partially attributed to the superoxide free radicals ( O 2 − ). A convenient and sensitive colorimetric method based on VB3-H 2 O 2 -TMB chromogenic system for H 2 O 2 and glutathione detection was fabricated with the linear ranges of 5.0–100.0 μM and 5.0–50.0 μM, respectively. In short, this work will not only bring new enlightenment on the physiological functions and practical applications in the analytical field of VB3, but also provide a new type of structural reference for small-molecule enzyme mimics.
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JOURNAL OF SEPARATION SCIENCE
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2022
·Vol. 45
·期 12
·2045-2054
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IF 3.614
Yali Chen,Ling Xia,Xiaohua Xiao,Gongke Li
Cosmetics that have medicinal effects, including anti-inflammatory and antioxidant, have become a daily care routine consumption. The peptide additives, such as carnosine and nicotinamide, were frequently used to realize these medicinal effects. To accomplish rapid and effective quantitation of carnosine and niacinamide in cosmetics, capillary zone electrophoresis was executed in cyclic olefin copolymer microchips having both dynamic and static coatings. The static coating of cyclic olefin copolymer microchannel was constructed from bovine serum albumin adsorption, immobilization, and active site closure, while the dynamic coating was formed by adding surfactant into running buffer of capillary zone electrophoresis. The static coating can improve the hydrophilicity of cyclic olefin copolymer surface and avoid nonspecific peptide adsorption. The dynamic coating of sodium dodecyl sulfate in running buffer proved to be useful in flow velocity adjustment and the column efficiency enhancement in the capillary zone electrophoresis separation channel of the cyclic olefin copolymer microchip device. A separation resolution up to 4.24 on the mixture of carnosine and nicotinamide was obtained. Moreover, an analysis method was established and applied to simultaneous carnosine and nicotinamide determination in a liquid whitening essence and a solid antiglycation pill, and the results were verified by comparison with high-performance liquid chromatography methods, indicating its potential in complex sample analysis.
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