TRENDS IN BIOTECHNOLOGY
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2026
·
IF 16.6
Dianjiang Yu,Haofeng Chen,Yang Zhang,Yueyang Chen,Hongwei Chen,Shujin Lin,Junyi Wang,Haoyi Yang,Xueping Ling,Ning He,Jifeng Yuan
Microbial synthesis of chemicals still faces low production due to the complicated metabolic networks, which generally cannot be tackled by rational engineering strategies. In this study, we report on a workflow, biosensor-guided indexing of genome evolution (BRIDGE), to obtain marker-free engineered strains with improved chemical production. We adopt the BRIDGE system to achieve high-level protocatechuic acid (PCA) production in Pseudomonas putida , reaching 4.67 g l −1 in shake flasks. Transcriptomics analysis reveals a synergy between metabolic pathway activation and the secretion system, allowing PCA overproduction. Furthermore, we demonstrate that the engineered P. putida can serve as a platform for de novo synthesis of other chemicals with high titers, achieving 2.97 g l −1 cis,cis -muconate in shake flasks. Since the BRIDGE system gives marker-free engineered strains, it would greatly favor downstream industrial applications. Considering the great variety of available biosensors, we envision that our BRIDGE approach will accelerate other microbial synthesis processes with enhanced productivity.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2026
·
IF 6.2
Yan Wang,Yitian Yu,Nuohan Xu,Zhenyan Zhang,Bingfeng Chen,Minglong Song,Qi Zhang,Tingzhang Wang,Yunting Ma,Tao Lu,Liwei Sun,Haifeng Qian
B vitamins (VBs) are essential micronutrients, yet their microbial production across natural habitats remains poorly understood. We analyzed 126,931 metagenome-assembled genomes to map the distribution of VB producers and constructed a database of 87 VB synthesis genes, identifying VB-independent producers and their environmental drivers. Soil and freshwater habitats harbored the greatest diversity of VB1, VB2, VB7, VB9, and VB12 producers, whereas marine systems contained fewer. Biosynthetic capacity was rare for VB5 and was limited for VB3 and VB6. Gammaproteobacteria are prolific producers, synthesizing up to eight VB types. Soil sulfur content and conductivity significantly enhanced VB production potential, linking beneficial microbes to improved soil health. We further validated VB3 and VB7 synthesis in Acinetobacter kookii, supporting our predictive framework. Because the identified producers include both beneficial and pathogenic taxa, careful evaluation is required. This work provides a robust gene database and a foundation for optimizing microbial VB production in agriculture and biotechnology.
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JOURNAL OF SEPARATION SCIENCE
·
2026
·Vol. 49
·期 7
·e70489
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IF 3.6
Jiaheng Yan,Yifan Xu,Yihui Chen,Zhicheng Bai,Jianfeng He,Meikang Lei,Xiaoqiang Qiao,Tingting Wang
Quantifying hydrophilic B vitamins (B1 [(thiamine], B2 [riboflavin], and B3 [nicotinic acid]) in complex functional food matrices presents a significant challenge. Conventional sorbents, such as C18, often exhibit poor retention and low recoveries (50%–60%) due to weak hydrophilic interactions. To efficiently extract these target analytes from functional foods, a new sorbent with tailored hydrophilic affinity has been developed by incorporating Co-cyromazine complexes onto halloysite nanotubes. This composite was employed as the sorbent for a spin-tip solid-phase extraction method, which was coupled with liquid chromatography-tandem mass spectrometry analysis. Following comprehensive optimization and validation, the method demonstrates simplicity, accuracy, sensitivity, and cost-effectiveness. It requires only 10 mg (or 10 µL) of functional food sample, while delivering high recoveries (81.3%–107.8%) with good precision (relative standard deviations ≤10.7%). The limits of detection ranged from 0.4 to 2.5 ng/mL in liquid matrices and from 0.7 to 8.7 ng/g in solid matrices. Practical applicability was confirmed through the successful quantification of vitamins B1, B2, and B3 in various commercial functional foods. Consequently, the developed method provides a reliable and promising analytical tool for the determination of B vitamins, with the potential for extension to other hydrophilic analytes in biological and food matrices.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2026
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IF 4.8
Shen Zhang,Zhuo Wang,Yuyu Guo
Although vitamin B12 (VB12) is an essential water-soluble vitamin for the human body, the adverse reaction by excessive use is also worth noting. Therefore, it is crucial to detect the accurate concentration of VB12. In this work, biomass-based carbon dots (BCDs) were prepared by a facile one-pot hydrothermal method from black rice and urea. Based on inner filter effect and static quenching mechanism, the BCDs suggested conspicuous sensitivity and specificity for VB12 detection. The linear ranges were 0.5–100 μM and 0–50 μM under dual fluorescence and colorimetric modes, respectively. Additionally, the obvious color change by addition of VB12 in BCDs solution ensured the feasibility of visual experiments with smartphone assistance. The portable determination system maintained exceptional operability, quick analysis and excellent precision. Combined with the outstanding performance in real samples, this study offers a novel tactic for pharmaceutical analysis based on fluorescence/colorimetric/visualization modes.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2026
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IF 5.4
Qiao Ding,Yuhang Liu,Ning Li,Bo Wu,Qing Cheng,Qi Zhao,Tong Liu,Rui Wang,Deyu Li
Dye-containing acid sulfate copper plating baths frequently suffer from severe dull deposits in the low-current-density (LCD) region due to the overpowering synergistic inhibition of polyether suppressors and dyes. To address this challenge, we developed a novel nicotinic acid-modified imidazole–epichlorohydrin complex (IEN1.5) designed to uniquely preserve the activity of the bis(3-sulfopropyl) disulfide (SPS) accelerator. Through a combination of electrochemical analyses, macroscopic plating tests, and DFT/MD simulations, we demonstrate that IEN1.5 physically interacts with SPS via specific non-covalent bonds rather than acting as a standalone, competing brightener. This original molecular strategy successfully breaks the severe LCD suppression, significantly enhancing deposit brightness and strengthening the Cu(111) crystallographic texture. Crucially, by preventing the detrimental over-coordination to Cu⁺ intermediates, IEN1.5 achieves unprecedented bath stability, reducing apparent SPS consumption to a mere 0.9% over 100 cyclic voltammetric stripping (CVS) cycles. These findings provide a highly original and robust mechanistic framework for designing stable, high-efficiency additive systems in advanced copper electrodeposition.
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FOOD CHEMISTRY
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2022
·
IF 9.231
Zhiqin Ding,Mengdie Cai,Weimin Gan,Ping Yuan,Lijun Wei,Xianglei Cheng
In this paper, a novel, selective and sensitive high performance liquid chromatography-UV photolysis-fluorescence detection (HPLC-UV-FLD) method for nitrosamines (NAs) is proposed based on terbium-doped carbon dots (Tb-CDs). Experimental studies have shown that nitrosamines can be degraded by UV photolysis with wavelength at 254 nm to produce NO 2 − , while NO 2 − can rapidly and specifically quench the fluorescence of Tb-CDs. Based on this principle, N -nitrosodimethylamine (NDMA), Nnitrosomorpholine (NMor), N -nitrosopyrrolidine (NPYR), N -nitrosodiethylamine (NDEA) and N -nitrosopiperidine (NPIP) are separated within 8 min by HPLC. These five kinds of NAs showed a good linear relationship in the range of 10–2000 μg/L, and the limit of detection was as low as 2.25 μg/L. The proposed method is successfully applied to the detection of sweet potato, lotus root, potato, water chestnut, tomato and cucumber. The sample recoveries range from 77.35 % to 119.3 %.
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ANALYTICAL LETTERS
·
2023
·
IF 2
Peng Qiu,Zhaorui Song
A simple and rapid fluorescence polarization immunoassay (FPIA) based on polyclonal antibodies is reported for the first time for the determination of nicotinic acid. First, six nicotinic acid analogues were coupled with fluorescein isothiocyanate isomer I (FITC) to obtain tracers. The influences of tracer structure on the performance of FPIA as well as the structure-activity relationships were explored. Subsequently, the tracer concentration and antibody dilution ratio on the performance of FPIA were investigated. For the optimal conditions, the detection range of FPIA was from 1.1 to 145.6 μg mL−1 and its IC20 value as the limit of detection (LOD) was 1.1 μg mL−1. The cross-reactivity of antibodies with common vitamins was evaluated. The intra-assay recovery was from 92.0% to 101.2% in food and tablets, with inter-assay recoveries between 92.0% and 103.8%. The developed FPIA provided good sensitivity and reproducibility and therefore provided efficient and economic screening for nicotinic acid in food and tablets.
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DYES AND PIGMENTS
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2022
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IF 5.122
Yujiao Tu,Ze Liu,Lei Jiang,Yingying Xiang,Fei Song,Lifen Meng,Xiuling Ji,Lin Yuan
New coccine is one of the most used red colorants in foods. Due to its cytotoxicity, quantitative determination is very necessary. Herein, the ratiometric fluorescent and UV absorption dual-mode nanosensor , derived from fangchinoline and o-phenylenediamine, was successfully prepared by hydrothermal way. The prepared carbon dots with three colors were purified with dialysis, thin layer chromatography and chromatographic column. Highly green fluorescence of Fan,N-CDs was quenched by new coccine with inner filter effect. The dependence of the ratiometric fluorescent response on new coccine concentrations was found linear over the range of 0–12.5 μM and 20–55 μM with LOD of 30.12 nM and 54.85 nm, respectively. The linearity of UV absorption ranged from 0 to 45 μM with of LOD 1.98 nM. In addition, the nanosensor was used to determine new coccine in energy drinks, carbonated beverage, gum and ketchup. It provides a new insight on the detection of new coccine by the dual-mode nanosensor.
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Nanomaterials
·
2022
·Vol. 12
·期 23
·4306
·
IF 5.719
Huifang Wu,Mengqi Xu,Yubing Chen,Haoliang Zhang,Yongjun Shen,Yanfeng Tang
The specific detection of oxytetracycline (OTC) residues is significant for food safety and environmental monitoring. However, rapid specific determination of OTC from various tetracyclines is still challenging due to their similar chemical structures. Here, nitrogen-doped carbon dots (NCDs) with excitation and pH-dependent optical properties and a high-fluorescence quantum yield were successfully synthesized, which were directly employed to fabricate a dual-response fluorescence probe by self-assembly with Eu3+(NCDs/Eu3+) for the ratiometric determination of OTC. The addition of OTC into the probe greatly enhances the characteristic emission of Eu3+due to the “antenna effect”, and the incorporation of NCDs into the probe further improves the Eu3+fluorescence by remarkably weakening the quenching effect caused by H2O molecules and efficiently shortening the distance of energy transfer from OTC to Eu3+. Meanwhile, the fluorescence of NCDs apparently decreases due to aggregation-caused quenching. The results demonstrate that a ratiometric detection of OTC (0.1–25 µM) with a detection limit of 29 nM based on the double response signals is achieved. Additionally, visual semi-quantitative assay of OTC can be realized with the naked eye under a 365 nm UV lamp according to the fluorescence color change of the as-fabricated probe. This probe exhibits acceptable specificity and anti-interference for OTC assay, holding promise for the fast detection of OTC in real water and milk samples.Keywords:oxytetracycline;nitrogen-doped carbon dots;lanthanide;ratiometric;visual analysis
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PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
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2023
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IF 7.8
Hongwei Ren,Yize Liu,Ruoyao Zhang,Tengda Zhao,Jing Han,Zhiyang Zheng,Erhong Duan
The sustainable development of carbon dioxide (CO 2 ) resource requires the realization of green and efficient capture for the fossil fuel CO 2 . However, the high operation costs and biological toxicity have hampered the application. Natural deep eutectic solvents (NADESs) are efficient and green CO 2 absorbent. In this manuscript, kinds of amino acid-based NADESs were prepared successfully to improve the absorption efficiency of CO 2 capture solvent. The chemical structure of NADESs was characterized, and the thermal stability was also measured. The CO 2 capture behavior of amino acid-based NADESs was investigated. With multi-reactive sites, unstable stage and more accessibility of alkyl structure on HBA (alkyl amino acids and aromatic amino acids, especial L -Ary), and less electronegativity on HBD (EG), L -Arg/EG NADESs had lower solvation effect ( β value, 1.286) and reduced viscosity (48.33 mpa.s at 60 ºC). It behaved better absorption capacity . The optimized conditions by single-factor analysis and Response surface methodology were L -Arg/EG at the mole ratio of 1:5, 30 °C and 200 mL/min, and the absorption could reach 0.81 molCO 2 /kgNADESs. Additionally, an insight into the reaction mechanism was investigated. It was found that hydrogen-bond structure played an important role in CO 2 capture. The broken of H-O···H-N formed by amino acids and ethylene glycol promoted the formation of new bonds of -(O=)C···NHR between L -Arg/EG and CO 2 , thus achieved higher CO 2 capture. The investigated proved that the green solvent amino acid-based NADESs could be efficient absorbents for CO 2 capture.
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Catalysts
·
2023
·Vol. 13
·期 2
·371
·
IF 3.9
Xue-Jiao Liu,Bao-Di Ma,Xiao-Mei Wu,Yi Xu
A recombinantE. coli, expressing nitrilase fromAcidovorax facilis72W with dual-site expression plasmid pRSFduet (E. colipRSF-AfNit2), was constructed. It showed higher soluble expression of nitrilase than that in the pET21a plasmid. The recombinant nitrilase can efficiently catalyze the hydrolysis of 3-cyanopyridine to nicotinic acid. The whole cells ofE. colipRSF-AfNit2 were immobilized by using sodium alginate/glutaraldehyde/polyethylene imine as the best immobilized reagents. The immobilized cells showed 95% activity recovery and excellent mechanical strength, with improved thermal stability and pH stability. They also retained 82% of initial activity after nearly two months of storage at 4 °C. A semi-continuous packed-bed bioreactor (sPBR) filled with the immobilized cells was studied for efficient production of nicotinic acid. After optimization, the highest space–time yield of 1576 g/(L·d) was obtained on 0.8 M substrate concentration at 2 mL/min of flow rate. The sPBR was repeatedly operated for 41 batches, keeping 100% conversion in the presence of 30 mM CaCl2. Finally, 95 g of nicotinic acid were obtained at 90% yield after separation and purification. The developed technology has potential application value.Keywords:nitrilase;cell immobilization;nicotinic acid;reaction optimization;semi-continuous packed-bed bioreactor
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JOURNAL OF AOAC INTERNATIONAL
·
2022
·Vol. 105
·期 5
·1350-1359
·
IF 2.028
Xiang Yingying,Song Fei,Jiang Lei,Liu Ze,Tu Yujiao
BackgroundVB12 is involved in the production of bone marrow red blood cells, preventing pernicious anemia and brain nerve damage.ObjectiveA large edible fungus Lactarius hatsudake (Lh) was used as a carbon source to produce fluorescent carbon dots (CDs) with high quantum yield and high photostability by a one-step hydrothermal method.MethodThe structure and optical properties of Lh-CDs were characterized by XRD, XPS, FTIR, and UV-Vis, and the stability of Lh-CDs was studied. The maximum excitation wavelength and emission wavelength of Lh-CDs were 324 and 408 nm, respectively, and the average fluorescence lifetime was 2.29 ns.ResultsThe linear range is 0∼20 μM (R2 = 0.9972) with a detection limit of 36.9 nM. At the same time, the mechanism of fluorescence quenching was internal filtration effect.ConclusionsIt showed that Lh-CDS had good selectivity, high sensitivity, and good anti-interference ability to VB12, which provided a new detection method for VB12.Highlights The fluorescence intensity of the LH-CDs could be effectively quenched by VB12.HighlightsThe fluorescence intensity of the LH-CDs could be effectively quenched by VB12.
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