Animal Nutrition
·
2024
·
IF 6.1
Yufeng Liu,Cheng Liu,Shuo Zhang,Jinming Hu,Meng M. Li,Guangyong Zhao
The objectives of the experiment were to compare the effects of rumen-protected taurine (RPT) and rumen-protected methionine (RPM) on the nitrogen (N) metabolism, plasma biochemical parameters, and metabolomics in beef steers and to clarify whether taurine plays similar roles as methionine (Met) in improving the regulation of N metabolism in beef steers. Six Simmental steers aged 12 months (liveweight 325 ± 7 kg) were used as experimental animals. The experimental treatments included a basal diet, the basal diet + 70.0 g/d RPT and the basal diet + 74.2 g/d RPM. The treatments were assigned in a replicated 3 × 3 Latin square design. Each experimental period included 15 d of adaptation and 5 d of sampling. The results showed that supplementing the diet with RPT or RPM did not affect the apparent nutrient digestibility ( P > 0.05). Supplementing the diet with RPT or RPM increased the N retention ( P < 0.05) and the N utilization efficiency (NUE) ( P < 0.05) and decreased the urinary excretion of 3-Methylhistidine ( P < 0.05) and the estimated skeletal protein degradation rate ( P < 0.05). Supplementing the diet with RPT increased the plasma concentrations of taurine ( P < 0.001), cysteine ( P = 0.010), valine ( P = 0.013) and total non-essential amino acids (NEAA) ( P = 0.047) and tended to increase the plasma concentrations of essential amino acids (EAA) + NEAA ( P = 0.087), but it did not affect the plasma concentrations of total EAA ( P > 0.05). Supplementing the diet with RPM increased the plasma concentrations of methionine ( P = 0.033), lysine ( P = 0.047), cysteine ( P = 0.007), leucine ( P = 0.046), isoleucine ( P = 0.046), valine ( P = 0.034), total EAA ( P = 0.028), total NEAA ( P = 0.004) and EAA + NEAA ( P = 0.004). The plasma metabolomics profiling revealed that supplementing the diet with RPT upregulated the plasma concentrations of taurine ( P < 0.001), L-Cysteine ( P = 0.004) and some amino acid (AA) analogues ( P < 0.05) and RPM upregulated the plasma concentrations of Met ( P = 0.021), L-Isoleucine ( P = 0.036), L-Tryptophan ( P = 0.006) and some AA analogues ( P < 0.05). In conclusion, taurine has similar impacts to Met in improving the N retention and the NUE in beef steers. Taurine deficiency is one of the main reasons that the NUE of beef steers is low. Supplementation of the diet with taurine is beneficial to the N utilization in beef steers.
研究领域:
动物营养学
·
畜牧学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Animal Nutrition
·
2025
·
IF 7.5
Manman Fan,Jinming Hu,Cheng Liu,Shuo Zhang,Yufeng Liu,Guangyong Zhao
Taurine is a sulfur-containing nonproteinogenic amino acid. Recent studies have shown that taurine can improve rumen microbial crude protein (MCP) synthesis. This experiment aimed to investigate the action mechanism of taurine on rumen MCP synthesis and nitrogen (N) metabolism in beef steers using sodium sulfate (Na 2 SO 4 ) as a contrast. Six steers (bodyweight 506 ± 17 kg) were assigned to three experimental groups including a basal diet (control), a basal diet supplemented with 45 g taurine/d or 50 g Na 2 SO 4 /d, and were allocated in a replicated 3 × 3 Latin square design. The amounts of sulfur from taurine and Na 2 SO 4 were equal (11.38 g/d). The results showed that, compared with the control group, both taurine and Na 2 SO 4 increased ruminal production of MCP ( P < 0.05) by 37.50% and 29.17%, respectively, and increased the concentration of sulfide (S 2- ) ( P < 0.001). Both taurine and Na 2 SO 4 increased the digestibility of neutral detergent fiber ( P < 0.05). Taurine tended to increase ( P = 0.087) while Na 2 SO 4 decreased plasma urea concentration ( P = 0.049), with the taurine group exhibiting higher plasma urea concentrations than the Na 2 SO 4 group ( P = 0.003). Taurine did not affect urinary urea excretion ( P = 0.246) whereas Na 2 SO 4 decreased urinary urea excretion compared with the control group ( P = 0.002). Compared with the control group, both taurine and Na 2 SO 4 increased urinary allantoin excretion ( P < 0.05), total purine derivatives excretion ( P < 0.05), and the estimated rumen microbial N flow ( P < 0.05). The urinary urea excretion of the taurine group was higher than the Na 2 SO 4 group ( P = 0.019). Compared with the control group, taurine did not affect N excretion, N retention (NR) or N utilization efficiency (NUE) ( P > 0.10), but Na 2 SO 4 decreased urinary N excretion ( P = 0.018) and total N excretion ( P = 0.024), and increased NR ( P = 0.024) and NUE ( P = 0.022). No differences were found in NR and NUE between the taurine and Na 2 SO 4 groups ( P > 0.10). Taurine improved ruminal MCP synthesis by enriching the pathways associated with sulfur and amino acid metabolism while Na 2 SO 4 improved ruminal MCP synthesis by enriching pathways related to nucleotide and purine metabolism. In summary, both taurine and Na 2 SO 4 improved ruminal MCP synthesis by modulating different pathways. Taurine was less effective in decreasing total N excretion than Na 2 SO 4 but no differences in NR and NUE were found between the two groups of beef steers.
研究领域:
动物营养学
·
畜牧学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2022
·Vol. 15
·期 10
·2801-2809
·
IF 3.498
Wang Xiaofei,Zhang Lu,Ye Nengsheng,Xiang Yuhong
A new metal–organic framework heterostructure composite (MOF@MOF, ZIF-8@PCN-128Y) was synthesized by an internal extension growth method and used as a sensor for the detection of tetracycline (TC). In this method, TC was enriched by virtue of the adsorption capacity of ZIF-8 and detected by PCN-128Y acting as an optical tentacle in virtue of its luminescence advantage. TC was rapidly detected with sensitivity and selectivity, and the limit of detection was 60 nM. At the same time, this method has good linearity and a wide linear range. TC in milk and beef samples was determined by this sensor, and the recovery rate was 94.2–106.3%. This work provided guidance for the construction and application of the MOF@MOF fluorescence sensor.
研究领域:
材料科学
·
分析化学
✓ 已验证
DOI
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
·
2014
·
IF 2.803
Yan-Qing Wang,Hong-Mei Zhang,Jian Cao,Bo-Ping Tang
Interactions of bisphenol S, a new bisphenol analogue with bovine serum albumin and calf thymus DNA were investigated using different spectroscopic methods and molecular modeling calculation. According to the analysis of experimental and theoretical data, we concluded that hydrophobic interactions and hydrogen bonding primarily mediated the binding processes of bisphenol S with bovine serum albumin and DNA. In addition, the electrostatic force should not be excluded. Molecular modeling studies indicated that the binding site of bisphenol S to bovine serum albumin located in the subdomain IB, while bisphenol S was a groove binder of DNA. In addition, BPS did not obviously induce second structural changes of bovine serum albumin, but it induced a conformational change of calf thymus DNA.
研究领域:
生物化学
·
分子生物学
✓ 已验证
DOI
高影响因子
JOURNAL OF AGRICULTURAL SCIENCE
·
2018
·Vol. 156
·期 2
·272-281
·
IF 1.186
Q. Liu,C. Wang,Y. L Zhang,C. X. Pei,S. L Zhang,G. Guo,W. J. Huo,W. Z. Yang
The present study evaluated the effects of isovalerate supplementation on the development of the small intestinal mucosa in dairy calves. Forty-eight Chinese Holstein bull calves at 15 days of age and 45.1 ± 0.36 kg of body weight were assigned randomly to four groups. The treatments were control, low-isovalerate, moderate-isovalerate and high-isovalerate with 0, 3, 6 and 9 g isovalerate per calf per day, respectively. The study comprised 75 days with a 15-day adaptation period followed by a 60-day sampling period. Calves were weaned at 60 days of age. Six calves were chosen from each treatment at random and slaughtered at 30 and 90 days of age. The small intestine morphology and activities of amylase and trypsin improved significantly with increasing age. No interaction between treatments and age was observed. The small intestine length, mucosa layer thickness, villus height and crypt depth increased linearly with increasing isovalerate supplementation. However, the ratio of villus height to crypt depth was not affected by treatment. Activities of amylase and trypsin increased linearly. The lactase activity increased linearly during the 75-day period and for pre-weaned calves but was unaltered for post-weaned calves. The relative mRNA expressions of growth hormone receptor, insulin-like growth factor-1 receptor and sodium-glucose co-transporter-1 in the small intestine mucosa increased linearly, and a similar pattern was observed for the expression of peptide transporter-1 in the duodenum and proximal jejunum. The results suggested that small intestine development was promoted by isovalerate in a dose-dependent manner.
研究领域:
动物营养学
·
胃肠生理学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
Food Chemistry-X
·
2024
·
IF 6.5
Wentao Qian,Xiaobing Wang,Hongliang Li,Yinhua Zhu,Pengjie Wang,Xiaolu Geng,Jinhui Yang,Huiyuan Guo,Menghui Wang,Chong Chen
Introducing Holstein cows on Qinghai-Tibetan Plateau is a potential solution to enhance local milk production. However, the relationship between milk quality and altitude in China remains unknown. Therefore, the components and plasmin (PL) system of raw milk from different altitudes (sea level, 1600, 2700, and 3800 m) were investigated. The daily milk production of Holstein cows and PL activity decreased as the altitude increased. However, the components content of raw milk, plasminogen (PLG)/PL ratio, activities of PLG and plasmin activator (PA) increased with altitude. The pasteurization resulted a significant decrease in PA activity of all milk and a significant increase in PL activity in milk collected at higher altitudes (2700 and 3800 m), suggesting the pasteurization was unsuitable for preserving milk at higher altitudes. This study offered references for the production and storage of milk after introducing Holstein cows on Qinghai-Tibetan Plateau.
研究领域:
农业
·
乳品科学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Yimeng Ren,Longzhu Zhou,Chaohua Tang,Jing Li,Yujie Shi,Qingyu Zhao,Junmin Zhang,Yueyu Bai
Jiaxian Red cattle is an indigenous, high quality cattle breed with marbled beef in China. We investigated the changes in lipid distribution, volatile compound and taste in Biceps femoris , Supraspinatus and Longissimus dorsi of Jiaxian Red cattle to further explore the quality characteristics of beef muscles with high lipid deposition. Neutral lipids in high-lipid-depositing beef had a dominant content percentage; in addition, 24 triglyceride species varied significantly. Longissimus dorsi lipid was more non-polar, with the highest total lipid and the lowest phospholipid content. The distribution of neutral lipids and glycerophospholipids was analyzed. Triglyceride was the lipid class that had the highest monounsaturated fatty acid percentage (52.44 %-55.88 %). Saturated and mono/polyunsaturated fatty acid classes content derived from neutral lipid increased in Longissimus dorsi , while that derived from glycerophospholipid increased in Supraspinatus. N-3 fatty acids content had low levels in Longissimus dorsi. Triglycerides containing oleic acid and linoleic acid had a significant positive correlation with differential aldehydes. These findings provide valuable insights into the lipid profile of beef with high lipid deposition, offering a foundation for future enhancements in beef quality.
研究领域:
食品科学
·
动物学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Yuanfeng Zhao,Jingrui Zhou,Wenju Luo,Lingling Jiang,Fuzhan Song,Jiang Ran,Haoxiang Xu,Lu Lei,Rong Ai,Bo Yu
This study investigated breed-specific flavor characteristics in Guanling (GL), Sinan (SN), and Simmental-crossbred (XZ) cattle using UHPLC-MS/MS and GC × GC-TOF MS. Specifically, XZ had 73 and 48 differential metabolites compared to GL and SN, respectively, whereas GL and SN showed 31 discriminated metabolites. Notably, these differences were linked to flavor-related metabolic pathways, particularly beta-alanine, histidine, glutathione, and purine metabolism. Regarding the composition of volatile organic compounds (VOCs), GL displayed a higher ketone content (18.58 %), whereas XZ was enriched in alcohol (56.25 %). Key VOCs with the highest flavor contributions across all breeds were 2,3-Butanedione, (E)-2-nonenal, 2-ethyl-1-hexanol, and (E)-2-octenal. However, dimethyl sulfide contributed significantly to GL and SN flavors but not to XZ. Collectively, the 49 differential VOCs led to aroma profiles of breeds varied in “sweet”, “fruity”, “nutty”, and “waxy” characteristics. This comprehensive analysis of non-volatile metabolites and flavor-related VOCs revealed breed-specific profiles and crossbreeding effects, providing insights for improving meat quality.
研究领域:
食品科学
·
畜牧学
·
分析化学
·
代谢组学
·
风味化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 7
·1139
·
IF 5.1
Liusha Kuang,Enqi He,Lei Zhou,Aihua Lou,Yan Liu,Wei Quan,Qingwu Shen
Xiangxi Yellow Cattle, an indigenous Chinese livestock breed recognized for its exceptional nutrient composition and superior meat characteristics, has gained significant market preference among consumers. This investigation focused on evaluating physicochemical attributes, flavor development patterns, and bacterial population dynamics in chilled beef samples stored at 4 °C over an 8-day period. The research further examined interrelationships between meat parameters, predominant microbial species, and crucial aroma-active substances. Findings revealed a progressive elevation in lipid/protein oxidation levels, biogenic amine accumulation, and TVB-N values as microbial proliferation intensified toward the late storage phase. Microbial analysis demonstrated a final total viable count of 7.17 log10 (CFU/g), with bacterial community dominance distributed amongFirmicutes(58.15%),Proteobacteria(29.56%), andBacteroidota(12.05%). Notably,Brochothrix thermosphactaemerged as the primary spoilage organism in terminal storage phases. Volatile organic compounds (VOCs) in the beef during storage were analyzed, with a total of 66 compounds identified. The critical analysis identified 2,3-butanedione and 2-butanone as microbial metabolism-dependent substances showing strong correlations with meat quality parameters, emerging as freshness markers for beef evaluation. Importantly, the study highlighted the necessity for deeper investigation into microbial–VOC interactions, particularly considering the intricate bacterial ecosystems in aquatic environments. These outcomes enhance our understanding of spoilage mechanisms in Xiangxi Yellow Cattle beef while proposing practical approaches for microbial control in meat preservation systems.
研究领域:
食品科学
·
微生物学
·
动物科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Microfluidics and Nanofluidics
·
2019
·Vol. 23
·期 5
·1-9
·
IF 2.437
Yang Xiaohan,Weldetsadik Netsanet Tesfaye,Hayat Zafar,Fu Taotao,Jiang Shaokun,Zhu Chunying,Ma Youguang
The aim of this work was to experimentally examine apparent rheological properties of fluids in microchannels based on pressure drop. Pressure drops of Newtonian and non-Newtonian fluids in microchannels were measured using pressure sensors. The effects of flow rate, Reynolds number, and viscosity of fluids on the pressure drop of single phase flow in microchannels were investigated, and the measurements of pressure drop were compared with prediction models. For Newtonian fluids, the friction factor in the laminar regime is f/ 2 = 26/ Re . The results show that the pressure drop of Newtonian fluid is in good agreement with the prediction formula proposed by Cornish. However, the pressure drop of non-Newtonian fluid deviates from the theoretical prediction. A power-law is proposed for the relationship between the apparent viscosity of non-Newtonian fluid and the characteristic shear rate in a microchannel based on the pressure drop measurement. This relationship between the apparent viscosity and the characteristic shear rate in a specific microchannel is completely different from the rheological curve measured by cone-and-plate rheometer, due to the heterogeneous distribution of shear rates at the radial direction for non-Newtonian fluids flowing in a microchannel.
研究领域:
流体力学
·
微流控技术
·
工程学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Jinjin Tong,Zezheng Liu,Kexuan Zhou,Kaiming Wang,Shijiao Guo,Hua Zhang
The overuse of antibiotics in livestock production poses a significant threat to public health, primarily by accelerating the emergence of antibiotic-resistant bacteria—a critical global issue. Therefore, the development of effective alternatives to antibiotics is urgently required to address this problem. In this study, BLfcin-NPs@CS/β-GP thermosensitive hydrogels were developed by incorporating bovine lactoferricin-loaded chitosan nanoparticles into a chitosan/β-GP hydrogel matrix. Scanning electron microscopy (SEM) revealed the formation of a three-dimensional porous structure. Fourier-transform infrared (FTIR) spectroscopy confirmed successful synthesis, while X-ray diffraction (XRD) analysis indicated that nanoparticle incorporation did not alter the crystallinity of the hydrogel. The rheological properties, in vitro degradation, biocompatibility, and antibacterial activity of the composite hydrogels were systematically evaluated, and the results demonstrated that the composite hydrogels exhibited temperature sensitivity, enhanced degradation rates, low cytotoxicity, and strong antibacterial activity. These findings suggest that BLfcin-NPs@CS/β-GP hydrogels present a promising alternative to antibiotics for treating bacterial infections, such as bovine mastitis, offering an innovative and effective strategy to reduce antibiotic use and mitigate the risk of antibiotic resistance in livestock production.
研究领域:
兽医学
·
生物材料学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2018
·
IF 5.091
Yuan Xu,Yuhai Tang,Yayun Zhao,Ruixia Gao,Junjie Zhang,Delai Fu,Zhaolun Li,Hecheng Li,Xiaoshuang Tang
Novel magnetic molecularly imprinted nanomaterials (DA + BSA-MMIPs) were prepared adopting bovine serum albumin (BSA) and dopamine as bifunctional monomers for the first time. Besides the role of assistant functional monomer, BSA can exclude the proteins with like charges and promote low molecular weight tetracyclines to be adsorbed. Thus, the DA + BSA-MMIPs could fulfil the selective separation of tetracyclines directly from milk samples. The characteristics, polymerization conditions, and adsorption performances of the resultant nanomaterials were investigated in detail. In addition of uniform imprinting layers, stable crystalline phase, and good magnetism of the DA + BSA-MMIPs, they have rapid binding kinetic, high adsorption capacity, and favorable reusability. The imprinted nanomaterials were coupled with HPLC to selectively extract and determine trace tetracyclines from untreated milk samples. The recoveries of tetracyclines are in the range of 84.1–95.8% with relative standard deviations of less than 6.7%. The developed method is especially suitable for the selective enrichment and detection of target compounds directly from a complex sample with proteins.
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）