ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2026
·
IF 4.1
Tang Xiaolu,Li Quan,Yang Yue,Wu Meilin,Liu Qingqing,Mo Zhihong
Protein-rich foods release biogenic amines (BAs) during prolonged storage, and these compounds serve as key biomarkers of food spoilage. Current separation-based methods for detecting BAs in food require sophisticated instrumentation and laborious sample pretreatment procedures. The array-sensing strategy, which mimics biological olfaction, provides a simple and rapid approach for the simultaneous detection of multiple BAs without the need for separation. Here, we constructed a multidimensional fluorescent sensor array using porphyrin-functionalized carbon dots (P-CDs) and their composites with metal ions (M-P-CDs), thereby incorporating the excitation wavelength as an additional signal dimension. This design reduces the number of physical sensors required while maintaining high discrimination capability, thereby overcoming a key limitation of conventional sensor arrays that depend solely on increasing the number of sensors. With the support of chemometric and machine learning methods, the sensor array successfully discriminated among ten BAs. We achieved quantitative detection of putrescine, histamine, and tyramine, determining their limits of detection (LODs) to be 2.37, 2.96, and 1.55 mg/L, respectively. The sensor array exhibited excellent stability (i.e., no significant fluorescence change over 30 days) and high selectivity against common interfering substances. Validation experiments on milk samples demonstrated the array’s ability to reliably discriminate specimens at different spoilage stages. This work establishes a novel multidimensional array-sensing strategy for detecting BAs, an approach that holds great potential for food safety applications. Graphical
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Yanan Liu,Yuhao Huang,Qileng Aori,Yu Wang,Sai Zhao,Zhen-Lin Xu
Antibacterial hydrogel packaging often adheres poorly to hydrophobic prefabricated meat surfaces. Metal-polyphenol networks, combining metal ion antibacterial properties with polyphenol adhesion, offer promising preservation potential. In this study, tannic acid -Fe III metal polyphenol network-coated gallic acid -loaded ovalbumin/chitosan nanoparticles with adhesion and sustainable antibacterial properties were prepared and embedded into the gelatin hydrogel to elongate the shelf life of prefabricated meat. The composite hydrogel exhibited high adhesion, with an average adhesion force of 1.4-fold ∼1.9-fold larger than the other groups. It also exhibited good antibacterial properties, the colony-forming units (CFU) of 4 type of bacteria was reduced by 0.69, 0.34, 1.05 and 0.90 log 10 (CFU/mL). The composite hydrogel delayed pH, total volatile basic nitrogen (TVB-N), microorganism growth, and the marginally acceptable limit was delayed by 4 days compared with the other groups in applications. These results demonstrate the composite hydrogel's significant potential as a preservation material for prefabricated meat.
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FISH & SHELLFISH IMMUNOLOGY
·
2026
·
IF 4.2
Wenmei Li,Chenchen Guo,Kaixin Wu,Qunrui Xie,Jianye Yang,Aiwen Shen,Qin Liu,Yuhong Ren
Unmethylated cytosine-guanine oligodeoxyribonucleotides (CpG ODNs) are potent immunostimulants via toll-like receptor 9 (TLR9) recognition, but their application is limited by nuclease degradation and high costs. Herein, a designed ssDNA fragment (1826-F) was circularized with T4 DNA ligase and amplified by rolling-circle amplification (RCA) with Phi29 polymerase, self-assembling into RCA-1826 nanoparticles with a mean size of 63.05 nm and a zeta potential of −5.75 mV. Nuclease resistance assays were performed to evaluate degradation stability, showing RCA-1826 degraded slowly over 24 h while linear control 1826-R was fully degraded within 6 h. In vitro , murine macrophages (RAW264.7) and zebrafish liver cells (ZFL) were treated with RCA-1826, and the results demonstrated that inflammatory factor expression was significantly upregulated, verifying its immunostimulatory activity. In vivo , administration of 1 μg RCA-1826 induced robust innate immunity in zebrafish. Combined with inactivated vaccine, RCA-1826 acted as an effective adjuvant, conferring 94.4% protection against Edwardsiella piscicida . This protection rate is 11.1% higher than that of the sulfur-modified ODN1826-adjuvanted vaccine (83.3%). Overall, RCA-based synthesis enables stable, immunogenic CpG DNA nanoparticles, highlighting RCA-1826 as a promising candidate for E. piscicida prevention in aquaculture.
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BIOMATERIALS
·
2026
·
IF 13.6
Zheng Deng,Xirui Wu,Tianhao Huang,Huiyuan Chen,Zhuochao Wu,Li Liu,Yuqian Liu,Chunjie Wang,Zijian Xiong,Zhuang Liu,Guangbao Yang
Intracellular polyamine metabolic homeostasis is closely linked to therapeutic outcomes in cancer. However, their intrinsic antitumor mechanisms have not been fully clarified. Herein, we demonstrate that the catabolism of spermine (Spm) and spermidine (Spd) mediated by polyamine oxidase directly generates two potent cytotoxic metabolites, namely acrolein and hydrogen peroxide. These metabolites act synergistically to trigger a marked elevation in intracellular reactive oxygen species (ROS), which in turn activate the caspase-1/GSDMD and caspase-3/GSDME signaling pathways, ultimately driving tumor cells into pyroptosis. Based on these findings, we develop a phenylboronic acid-functionalized sodium alginate-based hydrogel controlled-release system (Alg-PBA/Spd). This platform achieves sustained Spd release through coordination interactions and markedly inhibits tumor growth by inducing pyroptosis. Further studies demonstrate that the Alg-PBA/Spd hydrogel synergizes with radiotherapy (RT) to enhance radiosensitivity in an orthotopic breast cancer model and inhibit pulmonary metastasis. Moreover, this hydrogel enables co-delivery of αPD-L1 with sustained release, which significantly improves the response rate of immune checkpoint blockade therapy by inducing pyroptosis and remodeling the tumor immune microenvironment. Overall, this study not only elucidates a previously unrecognized mechanism underlying polyamine metabolism-driven pyroptosis but also offers a versatile platform to enhance the efficacy of RT and immunotherapy.
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JOURNAL OF CONTROLLED RELEASE
·
2025
·
IF 11.5
Peng Jiang,Yongnian Zeng,Wen Zhang,Jiapeng Lei,Xue Jiang,Wei Li
Reactive oxygen species (ROS)-based therapies have emerged as promising non-antibiotic strategies for treating infected wounds due to their broad-spectrum antimicrobial activity and low risk of resistance development. However, their clinical translation remains limited by low delivery efficiency, poor bacterial targeting, and uncontrolled post-treatment inflammation. To address these challenges, we reported a multifunctional bilayer microneedle patch (LFT/S-MN) designed for efficient bacterial eradication and enhanced wound healing. The patch integrates positively charged lactoferrin-based nanoparticles (LFT NPs) conjugated with a porphyrin sonosensitizer (TAPPFe) in the dissolvable base layer, and a pH-responsive tip layer loaded with the anti-inflammatory agent spermidine (SPD). Upon insertion into infected tissue, the released LFT NPs efficiently target and aggregate negatively charged bacteria, enabling robust ROS generation through synergistic chemodynamic and sonodynamic therapy (CDT/SDT) under ultrasound stimulation. Concurrently, the acidic wound microenvironment triggers the gradual degradation of the tip layer, allowing controlled SPD release for immunomodulation. In a murine P. aeruginosa -infected wound model, the LFT/S-MN patch significantly reduced bacterial load, suppressed inflammation, promoted collagen deposition and neovascularization, and accelerated wound closure. This work presents a promising non-antibiotic strategy for wound disinfection and regeneration through synergistic CDT/SDT and inflammation modulation.
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CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Yumo Chen,Shenyuan Ouyang,Bingjie Tong,Mengjiao Zhang,Meng Liu,Yiying Jia,Ting Ouyang,Sunkuan Hu,Guangju Zhao,Helin Xu,Kaili Mao
Ulcerative colitis (UC) is a chronic inflammatory bowel disease characterized by oxidative stress, and impaired intestinal barrier. Current therapies often fail to address the multifaceted nature of the disease. Herein, we developed a novel therapeutic small-molecule coacervate microdroplets formed by the multiple physical interaction between thioctic acid (TA) and spermidine (SP). TA-SP coacervate microdroplet is easily prepared by simply dispersing TA powders in SP solution, without addition of any toxic organic solvents. Besides, this TA-SP coacervate microdroplet exhibits the exceptional wetting and mucoadhesive properties. Crucially, upon rectal administration, it undergoes an in-situ phase transition from a liquid-like microdroplet to a dense hydrogel, significantly prolonging its retention at the colonic site. In DSS-induced murine colitis model, TA-SP coacervate microdroplets demonstrated the superior therapeutic efficacy compared to individual components, effectively alleviating disease severity, reducing colon shortening, and restoring histological architecture. Moreover, the pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) inside colitis colon was potently suppressed by TA-SP coacervate microdroplets via promoting the restorative M2 macrophage phenotype. Importantly, it repaired the gut mucosal barrier by restoring goblet cell function, mucin secretion, and tight junction proteins (ZO-1, OCLN and CLDN-5). Besides, TA-SP coacervate could effectively normalized gut microbial ecosystem of colitis mice via suppressing the expansion of pro-inflammatory pathobionts while promoting the recovery of beneficial commensals. Mechanistic studies revealed dual modes of action, that was, TA-SP not only functioned as a ROS scavenger to rebalance the oxidative-redox homeostasis via the Nrf2-Keap1 pathway but also severed as a potent autophagy-inducer to repair the epithelial homeostasis. By integrating physical barrier function with synergistic pharmacological activities, this self-assembled coacervate represents a promising and robust platform for the local treatment of ulcerative colitis.
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Bioactive Materials
·
2026
·
IF 20.3
Weiwei Yu,Xiang Li,Xufang Liu,Xinyi Hao,Wen Qin,Guoqing Qi,Gaopeng Dang,Ziyuan Tian,Shiyin Jin,Conrado Aparicio,Kaiyan Wang,Wen Niu,Lina Niu
Oral ulceration is a prevalent mucosal disorder, and its healing process is frequently hampered by poor local drug retention and inadequate mucosal adhesion. However, the underlying pathological mechanisms remain incompletely elucidated. In this study, transcriptomic sequencing revealed that ferroptosis serves as a key driver of oral ulcer progression. To address the limitations of conventional formulations in the wet and dynamic oral environment, we constructed an asymmetrically adhesive, ferroptosis-targeting, spermidine (SPD)-functionalized Janus hydrogel microneedle system (MN-HTSO-C). This system was fabricated via chemical grafting and dynamic Schiff base crosslinking, enabling targeted drug delivery into mucosal tissues. By specifically delivering SPD, this system effectively inhibited ferroptosis, reduced reactive oxygen species (ROS) accumulation, reprogrammed the local immune microenvironment, and thereby promoted angiogenesis and epithelial regeneration. Our work not only identifies a novel pathological mechanism but also proposes an integrated therapeutic strategy that combines targeted delivery, immunomodulation, and ferroptosis inhibition, providing a new direction for the treatment of oral ulcers and other ferroptosis-related mucosal diseases.
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PLoS One
·
2026
·Vol. 21
·期 1
·e0339776
·
IF 2.6
Keming Ying,Han Xue,Shenheng Du,Xin Wang,Xiaoyun Lei
N1,N12-diacetylspermine (DiAcSpm), a promising biomarker for cancer diagnosis, presents significant quantification challenges due to the structural homology within the polyamine family. To address this issue, we engineered a molecularly imprinted monolithic (MIM) column functionalized with biomimetic phosphorylcholine (PC) as functional monomer for the selective recognition of DiAcSpm in human urine. The zwitterionic polymer was synthesized via thermally initiated polymerization, with its morphology and pore architecture characterized through scanning electron microscopy (SEM) and brunauer-emmett-teller (BET) analysis. After optimizing capillary microextraction (CME) parameters, the MIM demonstrated a broad linear response (10–500 μM), a low detection limit (3.3 μM, S/N = 3), and high recoveries (76.8–91.2%) when coupled with HPLC-UV analysis. The biomimetic PC-based recognition significantly improved selectivity against key structural analogs, such as spermine, in complex biological matrices. This study underscores the potential of zwitterionic-based MIMs as a robust and efficient platform for the sensitive and selective monitoring of acetylated polyamines in clinical settings.
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Food Analytical Methods
·
2023
·Vol. 16
·期 5
·909-917
·
IF 2.9
Xu Yanfen,Lu Ying,Yu Jianna,Liu Wen,Jing Guoxing,Li Wenshan,Liu Wenjie
Tuna is one of the most popular fish and therefore among the most commercially valuable seafood. However, inadequate refrigeration during the whole supply chain causes serious economic loss and food poisoning. One important result of tuna spoilage is the accumulation of biogenic amines such as histamine, which can be used as a sensitive indicator for Tuna freshness. Thus, the development of rapid, sensitive, and economic analytical methods for biogenic amines in Tuna is highly desired. This research developed a method that combines high-performance liquid chromatography (HPLC) and electrospray ionization ion mobility spectrometry (ESI-IMS) to detect 7 kinds of biogenic amines including tryptamine, tyramine, spermidine, cadaverine, β-phenylethylamine, putrescine, and histamine in tuna. The limit of detections (LODs) for 7 biogenic amines are 0.01, 0.02, 0.02, 0.17, 0.03, 0.04, and 0.15 mg L −1 respectively. The linear range of histamine and spermidine is 2–100 mg kg −1 , and the linear range of other biogenic amines is 0.5–10 mg L −1 . The recoveries of biogenic amines are between 69.39 and 112.65%. Compared to the traditional HPLC-based analytical method, the proposed method does not involve any derivatization steps, which greatly decreased the sample preparation time and cost. Another advantage of the developed method is the supplementary separation to the HPLC, in which coeluted biogenic amines can clearly be separated in the ion mobility dimension. The proposed method is applied to determining the concentration of biogenic amines in tuna storage, which showed that this method can quickly and accurately monitor the changes of biogenic amines in tuna during storage.
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NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 16
·7588-7594
·
IF 3.3
Jianfeng Yan,Quanbin Fu,Shikai Zhang,Xianbao Shi,Yuanhong Zhang,Juying Hou,Junling Duan,Shiyun Ai
As a kind of organic basic compound containing nitrogen, biogenic amine is released from food spoilage and considered as an indicator for monitoring food freshness. Herein, an excellent dual-emission ratiometric fluorescent sensor for eight kinds of biogenic amines was established via the facile mixing of pH-responsive carbon dots and Rhodamine B. The developed sensor shows a rapid response (10 s), low limit of detection (0.733–3.943 μM) and a wide linear range (0–150 μM). More importantly, a portable fluorescent filter paper was prepared by simple dispersing the filter paper in a solution mixture of carbon dots and Rhodamine B. The obtained fluorescent filter paper based sensor showed an obvious color change in the presence of biogenic amine vapor derived from shrimp and mushroom. The results revealed that this sensor could realize the on-site and visual detection of biogenic amines in real food samples, making it suitable for potential application in food safety monitoring.
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Nanomedicine-Nanotechnology Biology and Medicine
·
2022
·
IF 6.458
Qiming Yin,Xiang Song,Peng Yang,Wen Yang,Xinyu Li,Xuejun Wang,Shengqi Wang
Liver injury caused by hepatitis is the pathological basis of varied hepatic diseases with high morbidity and mortality. Although siRNA appears promising in therapeutics of hepatitis, efficient and safe delivery remains a challenge. In this study, we developed a new strategy of incorporating glycyrrhizic acid (GA) and polyene phosphatidylcholine (PPC) into lipid nanoparticles (GA/PPC-modified LNPs), which was capable of promoting cellular uptake, enhancing gene-silencing, reducing cytotoxicity and improving siRNA stability. GA/PPC-modified LNP and siRNA lipoplex targeting NF-κB, a key mediator of inflammation, mitigates acute liver injury, as assessed by liver histology , hematological and pro-inflammatory cytokine analysis. Furthermore, GA/PPC-modified LNPs reveal efficiently intracellular delivery of antisense oligonucleotides (ASOs) and mRNA inhibiting viral infection. In conclusion, GA/PPC-modified LNPs could be used as a promising delivery system for nucleic acid-based therapy.
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Biomass Conversion and Biorefinery
·
2023
·
IF 4
Si Lin,Hou Juying,Lv Wei,Hou Qin,Xu Zhixiang,Ai Shiyun
In this paper, lignocellulose components were separated under mild conditions to achieve hierarchical utilization of biomass. Cellulose was retained in cellulose pulp with highly crystalline structure, and lignin powder was recovered, which offered a possibility for the high-value utilization of lignocellulosic biomass. The extracted lignin (EL) was carbonized and activated to prepare lignin-based porous carbon microspheres (LPCMs) and further used as a substrate to interact with fluorescein-5-isothiocyanate (5-FITC) for the detection of biogenic amines (BAs). The fluorescence of 5-FITC was found to be quenched in the presence of LPCMs. And then, upon addition of BAs, 5-FITC was released from the surface of LPCMs, which resulted in the fluorescence recovery of 5-FITC. Thus, a novel “turn off–on” fluorescent probe was constructed for sensitive and visual detection of BAs. Meanwhile, the retained cellulose skeleton structure was used to prepare a smart identification label for real-time and visual evaluation of the freshness of pork and shrimp. This work realized the improvement of comprehensive utilization rate of lignocellulose and developed a rapid, efficient, and sustainable method which combined natural lignocellulose materials with green analytical technology for visually evaluating food quality.
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危险品化学品经营许可证（带存储）