JOURNAL OF SEPARATION SCIENCE
·
2026
·Vol. 49
·期 7
·e70470
·
IF 3.6
Hanbing Li,Jie Wang,Yuyang Li,Yaming Li,Lei Yan,Sumei Li,Dayi Zhang,Yixuan Liang,Danyang Li,Xiaowei Wang,Sha Chen
A selective phase for tetracycline antibiotics (TAs) uptake was designed by incorporating [BMIM][PF 6 ]-functionalized ZIF-67-derived carbonaceous material into the diffusive gradients in a thin-films (DGT) device. This material, integrated in the binding gel of DGT (ta-DGT), exhibited specifically strong affinity toward tetracycline (TC), oxytetracycline (OTC), and chlortetracycline (CTC), with adsorption capacities of 12.22, 11.81, and 13.78 µg/disc, respectively. The ta-DGT showed preferential uptake of TAs over coexisting antibiotics and organic contaminants, with adsorption ratios above 80% and stable performance over pH 4–9 and ionic strength of 0.001–0.1 M. Mechanistic analysis indicated that π–π electron donor–acceptor interactions and cation–π bonding facilitated the specific targeting of TAs onto ta-DGT. During 7 days of field deployment in a domestic wastewater treatment plant in Beijing, ta-DGT showed 5–40 times higher detectable concentrations than conventional HLB/XAD DGT devices. These results validated its sensitivity, reliability, and feasibility for the selective separation and quantification of TAs in complex aqueous matrices.
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Foods
·
2026
·Vol. 15
·期 4
·724
·
IF 6
Haonan Jiang,Rong Liu,Xuhui Huang,Lei Qin
Erythromycin, a widely utilized antibiotic in aquaculture, poses potential food safety risks through residues in fish products. However, research on the effects of thermal processing on its degradation remains limited. This study developed a sensitive detection method using UHPLC-Q-Exactive HF-X to quantify erythromycin and its degradation products, investigating influences of various thermal treatments and predicting additional degradants. Turbot meat samples spiked with erythromycin were processed via roasting, microwaving, deep frying, and boiling. Post-treatment degradants were identified, with potential metabolites forecasted through structural analysis. The results indicated that erythromycin rapidly degrades under all thermal treatments, with boiling and roasting promoting substantial formation of dehydration products (anhydroerythromycin A and erythromycin A enol ether). The content of N-demethylerythromycin A remained consistently low and stable (<6.67 ng/g). Additionally, five additional erythromycin degradants were screened out. The degradation pathways and product profiles of erythromycin varied depending on the thermal treatment, indicating that thermal processing does not eliminate erythromycin residues but rather transforms them into other substances. This study revealed the specific degradation pattern of erythromycin during thermal processing of fish meat, providing scientific evidence for identifying which harmful substances require priority monitoring in processed fish products, thereby enhancing the level of food safety assessment.
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Hui Wang,Chengqian Li,Xiaoyu Luo,Chenxi Jiang,Fei Shang,Zhonghong Li,Yong Han
Multi-emissive red fluorescence carbon dots (R-CDs) were synthesized using Stellaria pallida and urea precursors through a solvothermal method, based on which a ratiometric fluorescent sensor was constructed and integrated into a portable dual-mode sensing platform for determining the residual levels of Chlortetracycline Hydrochloride (CTC) and Hemin Chloride (Hemin) in food products. The ratiometric fluorescent sensor achieved limits of detection (LODs) were 13.00 × 10 −3 μM for CTC and 0.74 × 10 −3 μM for Hemin, while the visual detection platform provided LODs of 1.50 × 10 −3 μM and 0.53 × 10 −3 μM, respectively. The relative standard deviation of the spike recovery tests ranged from 0.69% to 1.12%. This work presents a green, low-cost, and highly sensitive dual-analyte sensing strategy combined with a visual readout, offering a reliable platform for on-site food safety monitoring.
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Food Chemistry-X
·
2026
·
IF 9.2
Xuqi Situ,Jiacong Li,Yeshun Zhang,Hui Yan
To meet the urgent demand for monitoring tetracycline residues in milk—a critical food for vulnerable groups a novel fiber-optic Localized Surface Plasmon Resonance (LSPR) biosensor was engineered using gold-nanoparticle-labeled DNA triple-helix switches. The sensor operates via a target-activated disassembly: tetracycline binding dissociates the immobilized triple-helix, releasing gold nanoparticles and inducing a measurable LSPR blue shift. This active displacement amplifies the signal via localized refractive-index changes, enabling rapid, label-free detection. The sensor achieved excellent performance, with a linear range of 5–100 ng/mL and a low detection limit of 0.28 ng/mL. When applied to real milk samples, it delivered excellent recovery rates of 96.75%–104.15% (RSD <5.1%) and showed no statistically significant difference ( p > 0.05) compared to HPLC reference analysis. Combining high sensitivity with a modular, engineering-flexible design, this platform not only provides a practical solution for on-site tetracycline screening in food safety but also holds significant potential for adapting to other antibiotics and environmental targets, extending its utility beyond dairy monitoring.
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TALANTA
·
2026
·
IF 6.7
Wei Zhang,Jinhui Wang,Jialu Xu,Yulong Xu,Haiyan Li,Yujun Jiang
The environmental persistence and inherent biodegradation resistance of pesticide residues pose a serious threat to ecosystems and human health, necessitating the development of efficient monitoring strategies. Herein, we decipher the fluorine-fluorine (F···F) interaction within an AIE-active, trifluoromethyl-functionalized covalent organic framework (PEFA-COF) and exploit this molecular recognition motif for the selective detection of trifluralin (TFR). Benefiting from abundant trifluoromethyl moieties and superior luminescence properties, the PEFA-COF enables specific TFR recognition through a synergistic interplay of electrostatic forces, hydrogen bonding, and F···F interactions. This recognition event triggers a pronounced fluorescence quenching response via the internal filtration effect (IFE), facilitating highly sensitive and selective TFR quantification. The constructed PEFA-COF-based fluorescent sensing platform was successfully validated in complex water and milk matrices, achieving an excellent fluorescence quenching coefficient and a detection limit as low as 0.06 μmol/L. Collectively, this work not only deciphers the critical role of F···F interaction in pesticide recognition but also advances the function-led design of AIE-active COFs toward practical and robust pesticide residue monitoring in food supply chains.
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Analysis & Sensing
·
2026
·Vol. 6
·期 5
·e70116
·
IF 3.3
Keren Chen,Lingyun Wang,Qingfang Zheng,Longjiao Zhu,Liangjuan Zhao,Wenjie Zheng,Wentao Xu
Kanamycin residues pose potential risks to food safety and public health, highlighting the need for rapid, low-cost, and sensitive detection methods. However, developing label-free aptasensors that integrate target recognition and signal output remains challenging. Herein, a label-free fluorescent aptasensor was developed using a SMART-derived fluorescent aptamer and a competitive-equilibrium biosensing mechanism. The fluorescent aptamer MC-DA-LG was employed as an integrated signal-output and kanamycin-responsive aptamer module. MC-DA-LG generated a fluorescence signal upon binding to the fluorescent molecule, whereas the addition of kanamycin resulted in a concentration-dependent fluorescence decrease, suggesting a shift in the aptamer–dye binding equilibrium. Circular dichroism spectroscopy indicated conformational changes in MC-DA-LG upon the addition of kanamycin or malachite green, while molecular docking suggested partially overlapping binding regions for the two small molecules, collectively supporting the proposed mechanism. Under optimized conditions, the biosensor showed a linear response from 0.1 to 10 μM, with an ultralow detection limit of 400 pM. Moreover, the biosensor exhibited favorable selectivity over other antibiotics and satisfactory recoveries of 94.1%–97.3% in milk and fetal bovine serum samples. This simple, low-cost, rapid, and ultrasensitive assay enables direct fluorescence detection without labeling, immobilization, or complex procedures, demonstrating the potential of SMART-tailored fluorescent aptamers for integrated label-free biosensing of kanamycin. Graphical A SMART-derived fluorescent aptamer was designed as an integrated recognition and signal-output module for label-free kanamycin detection. The aptamer first binds malachite green to generate fluorescence, while kanamycin disrupts the aptamer–dye equilibrium through competitive interaction, producing a measurable signal change. This rapid and low-cost biosensor enables sensitive kanamycin detection in milk and FBS samples.
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Engineering
·
2026
·
IF 12.2
Ning Shao,Rui Liu,Jingjing Gan,Yuanjin Zhao
Biocompatible hydrogels are highly valuable for wound management; however, improving their mechanical compatibility and achieving controlled drug release for dynamic wound treatment remain challenging. Inspired by skin structure and function, a novel mechanically-responsive hydrogel was developed using drug-loaded liposomes as structural units. The crosslinked hydrogel network was generated via free-radical polymerization of acrylamide, incorporating double-bond-functionalized liposomes as crosslinkers. Deformable liposomes endowed the hydrogel with improved mechanical properties and enabled controlled drug release in response to mechanical deformation. The rifampin-loaded mechanically-responsive hydrogel exhibited strong antimicrobial activity both in vitro and in vivo . In addition, anti-inflammatory effects and enhanced wound-healing properties were observed in dynamic wound environments. These findings indicate that mechanically-responsive skin-mimicking hydrogels offer a promising strategy for dynamic wound management.
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RSC Advances
·
2026
·Vol. 16
·期 6
·5501-5514
·
IF 4.6
Lian Yu,Baozi Lu,Zhen Liang,Dabin Wang,Jiarui Lu,Fengyi Wei,Cunzhen Liang
The slow rate of the Fe3+/Fe2+ cycle has decreased the efficiency of photo-Fenton technology for wastewater treatment. Here, a novel GO/MIL-100(Fe)@Fe3O4 heterostructure was constructed by growing Fe3O4 nanoparticles and MIL-100(Fe) onto GO, thus accelerating the Fe3+/Fe2+ cycle and improving catalytic activity, and it was used as a heterogeneous photo-Fenton catalyst for ERY degradation. The catalyst was fully characterized by TEM, XRD, Raman spectroscopy, XPS, N2 adsorption–desorption and UV-vis DRS. The influences of initial solution pH, H2O2 concentration and catalyst dosage on ERY degradation were investigated. Under optimal conditions (initial pH of 4.03, 10.0 mM H2O2, 0.1 g L−1 catalyst), the GO/MIL-100(Fe)@Fe3O4 + H2O2 + vis system removed 97.3% of ERY (0.1 mmol L−1) within 60 min, and the pseudo-first-order rate constant was 0.0575 min−1. Furthermore, the catalyst showed favorable stability, maintaining excellent catalytic activity even after 4 cycles. A possible degradation mechanism of ERY was proposed based on the results of characterization, ERY degradation, and reactive species analysis. Fe3O4, MIL-100(Fe) and GO in the catalyst showed synergistic effects; the photo-induced electrons from MIL-100(Fe) could be immediately transferred to Fe3O4, promoting Fe3+ reduction to Fe2+ in the photo-Fenton reaction. GO in the catalyst could promote electron transfer from MIL-100(Fe) and organics to Fe3O4, significantly promoting the Fe3+/Fe2+ cycle.
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.1
Chenyan Lu,Dajie Zou,Ning Zhang,Huye Li,Yanyan Jia,Huan Wang
In this study, the hexane extract of Asters tataricus was used as the carbon source, and ethylenediamine and 4-bromophenylene diamine were selected as dopants to prepare nitrogen and bromine co-doped carbon dots (N,Br-CDs) by the hydrothermal synthesis method. The obtained fluorescent probe has a uniform particle size distribution, good water solubility and high stability, with a fluorescence quantum yield of 48.43%. Under 420 nm excitation conditions, N,Br-CDs exhibited the strongest fluorescence emission at 513 nm, indicating that the sensing system was in the “ON” state. Subsequently, TA was introduced as a quencher to induce static quenching of the N,Br-CDs system, significantly weakening the fluorescence intensity and switching the sensing system from “ON” to “OFF”. Finally, after EM was introduced into the N,Br-CDs/TA system, EM preferentially formed a complex with TA and weakened its quenching effect on the quantum dots, thereby achieving fluorescence recovery and returning the sensing system from the “OFF” state to the “ON” state. Based on the above mechanism, an “ON-OFF-ON” fluorescence sensing system with N,Br-CDs as the luminescent substrate was constructed for the sensitive detection of TA and EM. The results showed that the system exhibited good linear response to TA, with the linear ranges being 1.50–22.94 μM and 34.88–348.42 μM, respectively, and the limits of detection being 0.2168 μM and 2.1797 μM, respectively (LOD, S/N = 3). The linear range for EM was 1.93–377.08 μM, with a detection limit of 0.6693 μM. Furthermore, the sensor system was capable of quantitatively determining TA and EM in human serum, Huangshui River water, and milk matrices, demonstrating its potential for broad application in environmental monitoring and bioanalysis.
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 1
·60
·
IF 5.3
Yang Yu Zhu,Li Gui Yong,Zhang Pu,Cui Linyun,Liu Huan Li,Chen Shi,Dai Xin
In this study, N-CDs were combined with Eu 3+ to construct a ratiometric fluorescence sensor (N-CDs@Eu 3+ ) for the determination of tetracycline (TC). The nitrogen-doped carbon dots (N-CDs) with good water solubility, excellent fluorescence performance, and high stability successfully prepared by using the leaves of Mahonia fortunei leaf as the carbon source and ethylenediamine as the nitrogen source through a one-step thermal solvent method. In the presence of TC, the fluorescence intensity of N-CDs at 510 nm was quenched due to fluorescence resonance energy transfer (FRET), while the fluorescence intensity of Eu 3+ at 616 nm was significantly enhanced through the antenna effect (AE). This ratiometric fluorescence sensor enables rapid, sensitive and highly selective detection of TC, with a detection limit as low as 7.5 nM. Moreover, a distinct color change from green to red can be observed under a 365 nm UV lamp. With the assistance of a smartphone sensing platform, visual semi-quantitative monitoring of TC was achieved. This method was successfully applied to the detection of TC in milk, pork, and honey, with recoveries ranging from 90.6% to 105.0%. This research demonstrates that this sensor holds potential application value in the field of real-time visualization detection and semi-quantitative determination of TC in biomedical and food safety sensing fields. Graphical abstract
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Yunyan Ren,Zhefeng Fan
Tobramycin (TOB) plays a considerable role in combating milk spoilage and preventing disease in dairy cows. However, overuse of TOB can lead to nephrotoxicity, ototoxicity, neuromuscular blockade, and hypersensitivity reactions. Here, nitrogen-doped carbon dots ( N -CDs) were prepared with ethylenediamine and citric acid, then molecularly imprinted layers were obtained by imprinting of surface on the N -CDs to prepare Nitrogen-doped carbon dot-based molecularly imprinted polymers ( N -CDs@MIPs). The fluorescence emission spectrum of this probe showed a linear enhancement with the TOB concentration in the 1–12 μM. Meanwhile, a detection limit of 99.2 nM was obtained. This probe was not affected by the structural analogs of the TOB and can show high sensitivity and selectivity compared to non-imprinted polymers ( N -CDs@NIPs). Therefore, it can be successfully used for the trace analysis of TOB in milk with advantages over other reported techniques such as liquid chromatography coupled with tandem mass spectrometry or various aptamer sensors.
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Cell Metabolism
·
2023
·
IF 29
Di Li,Ting Sun,Yongqing Tong,Juan Le,Qian Yao,Jun Tao,Hang Liu,Woer Jiao,Yufeng Mei,Jingru Chen,Zhongchun Liu,Gaohua Wang,Yan Li
Estradiol decline can result in depressive disorders in females; nevertheless, the causes of this decline are unclear. In this study, we isolated estradiol-degrading Klebsiella aerogenes from the feces of premenopausal females with depression. In mice, gavaging with this strain led to estradiol decline and depression-like behaviors. The gene encoding the estradiol-degrading enzyme in K. aerogenes was identified as 3β-hydroxysteroid dehydrogenase (3β-HSD). Heterologously expressing 3β-HSD resulted in Escherichia coli obtaining the ability to degrade estradiol. Gavaging mice with 3β-HSD-expressing E. coli decreased their serum estradiol levels, causing depression-like behaviors. The prevalence of K. aerogene and 3β-HSD was higher in premenopausal women with depression than in those without depression. These results suggest that the estradiol-degrading bacteria and 3β-HSD enzymes are potential intervention targets for depression treatment in premenopausal women.
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