Small
·
2010
·Vol. 7
·期 1
·87-94
·
IF 6.171
Yi-Lun Ying,Hai-Yan Wang,Todd C. Sutherland,Yi-Tao Long
An aptamer is a specific oligonucleotide sequence that spontaneously forms a secondary structure capable of selectively binding an analyte. An aptamer’s conformation is the key to specific binding of a target molecule, even in the case of very closely related targets. Nanopores are a sensitive tool for the single-molecule analysis of DNA, peptides, and proteins transporting through the pore. Herein, a single α -hemolysin natural nanopore is utilized to sense the conformational changes of an adenosine 5’-triphosphate (ATP)-binding aptamer (ABA). The known DNA sequence of the ABA is used as a model to develop real-time monitoring of molecular conformational changes that occur by binding targets. The native, folded ABA structure has a nanopore unfolding time of 4.17 ms, compared with 0.29 ms for the ABA:ATP complex. A complementary 14-mer strand, which binds the ABA sequence in the key nucleic acids responsible for folding, forms linear duplex DNA, resulting in a nanopore transit time of 0.50 ms and a higher capture probability than that of the folded ABA oligomer. Competition assays between the ABA:ATP and ABA:reporter complexes are carried out, and the results suggest that the ABA:ATP complex is formed preferentially. The nanopore allows for the detection of an ABA in its folded, ATP-bound, and linear conformations.
研究领域:
分子生物学
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生物传感器
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核酸化学
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单分子检测
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转化医学
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DOI
高影响因子
JOURNAL OF ELECTRONIC MATERIALS
·
2022
·Vol. 51
·期 9
·4846-4854
·
IF 2.047
Bo Lin,Wang Lei,Hou Yangbo,Li Fujin,Liu Sida,Zhang Ruipeng,Zuo Min,Zhao Degang
Ternary Cu 3 SbSe 4 thermoelectric material with a diamond-like structure exhibits good thermoelectric performance in the middle-temperature region. In this study, Cu 2.98 Co 0.02 SbSe 4 material with intrinsically low thermal conductivity was prepared by a melting–ball milling–hot pressing process. The maximum zT value for the Cu 2.98 Co 0.02 SbSe 4 sample was 0.7 at 650 K, which was about 190% higher than that of the pristine sample. The improvement in thermoelectric performance was ascribed to the realization of multi-scale features induced by ball milling. It was found that multiple scattering centers of phonons were formed via interfacial engineering, including dislocations, nano-holes and grain boundaries, which offers an applicable pathway for the reduction of lattice thermal conductivity. Graphical
研究领域:
材料科学
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凝聚态物理
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功能材料
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能源材料
·
半导体物理
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DOI
高影响因子
RSC Advances
·
2012
·Vol. 2
·期 14
·5983-5989
·
IF 0
Hong-Wen Gao,Dong-Dong Ma,Gang Xu
Many kinds of medicinal plants are used in the treatment of disease, and a proportion of these plants grow tenaciously in soils contaminated with heavy metals. An example of one such medicinal plant is the herb Thlaspi rotundifolium L., which accumulates selenium (Se), and has anti-oxidation, anti-aging, anti-cancer and immunity-adjusting properties, but also simultaneously hyperaccumulates harmful metals such as Cd and Zn from the soil. An interesting model named ‘digging metals@plant then backfilling’ has been suggested, where the metals are removed from the plant by acid treatment, then the nano-pores imprinted with the heavy metals are backfilled from the drinking water. Experimental studies of this idea indicate that it can remove unwanted substances from the herb itself and also from the drinking water, ensuring the safety of the herb tea. Additionally, the Se normally embedded in the herb was not noticeably lost. Thus, this model provides a sustainable solution for the disposal of heavy metals using metal-accumulating plants.
研究领域:
植物生理学
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环境毒理学
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食品科学
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天然药物化学
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环境修复
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生物医学工程
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农业资源利用
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DOI
CHINESE CHEMICAL LETTERS
·
2016
·Vol. 28
·期 1
·49-54
·
IF 1.947
Qi-Feng Li,Chao Wang,Xiao-Zheng Lan
Latent heat storage performance of a layered perovskite-type compound, 1-C 14 H 29 NH 3 ) 2 ZnCl 4 (C 14 Zn), embedded in a series of silica gel (SG) with pore sizes of d = 15–200 nm is investigated using differential scanning calorimetry (DSC), and powder X-ray diffractions (XRD). C 14 Zn in the nanopores of silica gel shows size-dependent phase transition temperature, enthalpy change and supercooling. They have a stable transition temperature and heat capacity at each size in a short-term thermal cycling. Similar X-ray diffraction patterns are observed for the nano-sized and the bulk C 14 Zn. The encapsulation of a phase change material in nanopores is a new way of tuning its thermal energy storage properties for a wider range of temperature regulation.
研究领域:
材料科学
·
热力学
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纳米科技
·
能源存储
·
功能材料
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）