JOURNAL OF FOOD PROCESS ENGINEERING
·
2018
·Vol. 42
·期 2
·e12976
·
IF 1.955
Ning Yang,Liang-Liang Xie,Chen Pan,Min-Feng Yuan,Zhi-Han Tao,Han-Ping Mao
Ubiquitous food security control is desirable. However, the analysis methods, such as those for milk, are highly based on manual analysis, leading to low efficiency. Thus, we report a multilayer paper-based microfluidic chip transforming chromatic readout to electric signals. First, colloidal gold immunochromatographic assays (GICA) are used to achieve the colorimetric occurrence. Secondly, the test (T) lines and control (C) lines are patterned to encode different EAN-8 barcode. Furthermore, accurate scanning is accomplished by the automatic threshold segmentation. These technologies make the digital determination of melamine (MEL) and chloramphenicol (CAP) by phone readily accessible. Results indicate that the accurate machine-scanning can be assured when MEL and CAP is above 30 ng/mL and 6 ng/mL in milk respectively. The detection time is under 8 min, and the stable storage life is over 90 days. Therefore, this simple chip allows rapid, machine-readable, electric-recordable diagnostic directly from chromatic assays with satisfying stability, specificity and accuracy. Practical applications This article illustrates a method used to determine the concentration of melamine and chloramphenicol in milk. When melamine and chloramphenicol is above 30 and 6 ng/ml in milk, the produced EAN-8 barcode can be recognized with high accurate level, compared with international standards, the results are acceptable and sensitive. This method is highly intellectual and environmentally friendly, with the disposable materials and instant smartphone-readable EAN-8 results patterns. This article demonstrates a multilayer paper chip based on EAN-8 encoding rules, colloidal gold nanoparticles, and immunochromatographic assays. The EAN-8 format assay is achieved by color-development occurrence of colloidal GICA in different encoding areas of EAN-8 code, and the analysis device, namely smartphone, is accessible by almost every individual. Therefore, this chip can pave a potential way for massive health control and digital automated analysis for daily life and industrial manufacture.
研究领域:
食品安全
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分析化学
·
生物医学工程
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Xirui Chen,Yujie Tu,Song Cheng,Xujing Guo,Tianying Lu,Yuqian Guo,Xiaolin Huang,Yonghua Xiong,Ben Zhong Tang
Existing single-signal-response fluorescent materials for biogenic amine detection have unsatisfactory detection performance. Herein, we developed a simple pH-sensitive aggregation-induced emission-active fluorogen (AIEgen) and demonstrated its potential for ratiometric monitoring of biogenic amines. This AIEgen, namely 4-(dimethylamino)styryl)quinoxalin-2(1 H )-one (ASQ), shows a typical donor (D)-acceptor (A) structure with nonmonotonic color and fluorescence responses to pH stimulation by its protonation/deprotonation on the D/A moieties. Subsequently, the H + ASQ-labeled paper chips were successfully prepared by physically depositing ASQ on qualitative filter paper, followed by the acidification by hydrochloric acid. Upon the exposure to ammonia vapour, the obtained H + ASQ-labeled paper chips exhibited obvious color and ratiometric fluorescence responses owing to the deprotonation of H + ASQ indicators, indicating that the proposed paper chips can be used for the detection of biological amines by bimodal colorimetry and ratiometric fluorescence. Under the optimal conditions, H + ASQ-labeled paper chip achieved the real-time, non-destructive, and visual monitoring of meat and seafood freshness by simultaneously quantify the biogenic amine index with high performance liquid chromatography (HPLC). The results of this work suggest that ratiometric AIEgen can serve as a promising alternative strategy to ensure accurate monitoring of biogenic amines and food freshness.
研究领域:
分析化学
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食品安全
·
生物传感
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DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Lu Yang,Tong Zhai,Jing Wang,Hong Zheng,Jing-Min Liu,Shuo Wang
To achieve high throughput detection of clenbuterol while reducing matrix interference, we developed a simple and integrated nano-inspection platform. Herein, based on the simplicity and convenience of paper chip, a multi-layer paper chip-chemiluminescence (CL) sensor was designed in the form of “48-well plate” type. The all-in-one paper chip-CL sensor has integrated the covalent organic frameworks (COFs) layer for sample matrix purification, high affinity nanobody layer for specific capture of target, and CL rapid response layer for the final determination. The signal readout was realized by the inhibitory effect of clenbuterol on the K 3 [Fe(CN) 6 ] CL system. Detection of clenbuterol in pork meat was verified by measuring the CL intensity and satisfactory recoveries (85.9 %–97.3 %) were achieved. 48 samples could be simultaneously detected on one-chip in one-test, favoring the high-throughput detection. Especially, the work explored a new pathway for design of portable analytical instruments to achieve immediate on-site detection of hazards.
研究领域:
分析化学
·
食品安全检测
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Ningning Li,Ying Zhou,Hao Sun,Nan Wang,Nan Yang,Peiyao Ren,Lanjun Fu,Yiming Zhang,Wei Liu,Yanjie Li,Juan Jin
Rapid and accurate glucose monitoring is extremely crucial for the health management of diabetic patients. Whereas the non-invasive detection of salivary glucose is promising alternative. In this study, a newly designed MOF/MIPs paper-based microfluidic biomimetic chip (MM-SGM-chips) was successfully constructed based on boron-affinity orientation surface imprinting strategy. The obtained chips demonstrated high adsorption capability (Q e = 213.40 mg·g −1 ), excellent selectivity (IF = 3.7), speedy detection (less than 30 min), and satisfied anti-interference ability for glucose. Moreover, the MM-SGM-chips could be capable of rapid and sensitive quantification of glucose level ranged from 0.1-3.2 mM through visual detection, with a limit of detection as low as 0.02 mM. Furthermore, the real clinical samples in daily period were collected for validating its correlation with serum glucose level. Present works demonstrated the promising potentiality of MM-SGM-chips for home self-test and even point-of-care testing clinical applications.
研究领域:
生物医学工程
·
分析化学
·
糖尿病研究
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DOI
高影响因子
Foods
·
2021
·Vol. 10
·期 11
·2810
·
IF 4.35
Xinpeng Yan,Zhong Zhang,Runguang Zhang,Tian Yang,Guoying Hao,Li Yuan,Xingbin Yang
Given the imperative of monitoring organophosphorus pesticides (OPs) residues in the ecosystem, here a novel, facile and sensitive fluorescence sensor is presented for the rapid detection of dimethoate. In this work, surface molecularly imprinted polymer (SMIP) and microfluidic technology had been introduced to enhance the selectivity and portability of the described methodology. Oil-soluble CdSe quantum dots (QDs) synthesized in a green way were used as fluorescent material for the selective detection of dimethoate on the basis of static quenching and photoinduced electron transfer mechanism. Among many kinds of paper materials, glass fiber paper was used as the novel substrate of paper chip due to low pristine fluorescence and better performance when combining CdSe QDs. In the process of molecular imprinting, the interaction between several functional monomers and dimethoate molecule was investigated and simulated theoretically by software to improve the selectivity of the sensor. Consequently, the fabricated novel detection platform could effectively respond to dimethoate in 10 min with the concentration range of 0.45–80 μmol/L and detection limit of 0.13 μmol/L. The recovery in the spiked experiment soybean sample was in an acceptable range (97.6–104.1%) and the accuracy was verified by gas chromatography-mass spectrometry, which signified the feasibility and potential in food sampling.Keywords:oil-soluble CdSe QDs;dimethoate;microfluidic paper chip;fluorescence sensor
研究领域:
分析化学
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食品安全
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产品关联
DOI
PubMed
高影响因子
Foods
·
2022
·Vol. 11
·期 7
·932
·
IF 5.561
Xujing Guo,Xirui Chen,Rui Chen,Yujie Tu,Tianying Lu,Yuqian Guo,Liang Guo,Yonghua Xiong,Xiaolin Huang,Ben Zhong Tang
Herein, we developed a paper-based smart sensing chip for the real-time, visual, and non-destructive monitoring of food freshness using a ratiometric aggregation-induced emission (AIE) luminogen (i.e., H+MQ, protonated 4-(triphenylamine)styryl)quinoxalin-2(1H)-one) as pH sensitive indicators. Upon exposure to amine vapors, the deprotonation of H+MQ occurs and triggers its color change from blue to yellow, with the fluorescence redshift from blue to amaranth. Consequently, we successfully achieved the sensitive detection of ammonia vapors by recording the bimodal color and fluorescence changes. Given the high sensitivity of H+MQ to ammonia vapor, a paper-based smart sensor chip was prepared by depositing H+MQ on the commercial qualitative filter paper through a physical deposition strategy. After being placed inside the sealed containers, the developed H+MQ-loaded paper chip was applied to the real-time monitoring of biogenic amine contents according to its color difference and ratio fluorescence change. The detection results were further compared with those obtained by the high-performance liquid chromatography method, which verified the feasibility of the designed paper chip for the food spoilage degree evaluation. Briefly, this work indicates that the designed H+MQ-loaded paper chip could be a promising approach for improving food freshness monitoring.Keywords:aggregation-induced emission;fluorescent sensor;paper chip;biogenic amines;food spoilage
研究领域:
分析化学
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食品安全
·
传感技术
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Chunhua Li,Xiao Xu,Fuli Wang,Yue Zhao,Yubo Shi,Xin Zhao,Jue Liu
It is significant to develop low-cost, sensitive, and portable sensing platform for point-of-care testing of ascorbic acid (AA) in food quality evaluation. Herein, a smart point-of-care (SPOC) sensor, consisting of fluorescent paper chip, 3D printed-accessories and smartphone, was developed for ultrasensitive and visual quantitative detection of AA. Fluorescent paper chip was made through printing silicon doped carbon dots (SiCDs)-Fe 3+ as “ink” onto filter paper, in which SiCDs emitting strong fluorescence signal quenched by Fe 3+ , and then recovered by AA owing to releasing –NH 2 /–OH and introducing defects on SiCDs. The smartphone with an application named “ AA-Tester ” was established to analyze AA and exhibited high sensitivity with low detection limits of 18.12 nmol/L. HPLC method was used to verify the SPOC sensor and obtained satisfactory accuracy with relative standard deviations of 0.79–2.31 %. The portable sensing platform integrated fluorescence sensor with smartphone-device will be favorable for ultrasensitive and on-the-spot detection of AA.
研究领域:
分析化学
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食品科学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 7
·2066-2071
·
IF 1.938
Wei Zhang,Bowei Li,Lingxin Chen,Yunqing Wang,Dingxue Gao,Xuehua Ma,Aiguo Wu
A simple and facile method has been demonstrated to fabricate low-cost surface enhanced Raman scattering (SERS) active microfluidic paper chips using a painting brush. This strategy solves the problem of mass production of highly reproducible SERS substrates without complicated or bulky micro- or nanofabrication instruments. Rhodamine 6G (R6G) was chosen as a probe molecule to evaluate the performance of the SERS active chip. To further demonstrate the possibility of this method's potential application in environmental monitoring, trace malachite green (MG) was successfully analyzed on this chip. The performance of our chips was desirable. The paper substrates with silver nanoparticles deposited by brush were found to be cost-efficient and highly sensitive (LOD for R6G and MG are 1 nM and 10 nM, respectively), and have good reproducibility (∼15% relative standard deviation).
研究领域:
分析化学
·
环境科学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Guobin Mao,Yuan Zeng,Chunmin Qiu,Guangmiao Ding,Leyao Li,Lixin Ma,Junbiao Dai,Wen Yin,Yingxin Ma
Accurately monitoring the freshness of high-protein foods has significant implications for both food safety and public welfare. Since a large amount of hydrogen sulfide (H 2 S) is produced during spoilage-related processes, abnormal H 2 S levels are often considered an important indicator of food spoilage. Therefore, we synthesized novel nanoparticles (NPs) containing Silicon (Si) dots and CdTe quantum dots to accurately assess the amount of sulfide ions (S 2− ) and thus the quality of high-protein foods in the early stage of storage. As the concentration of S 2− increased, the fluorescence intensity of Si/CdTe NPs at λ em = 488 nm increased, while the fluorescence intensity at λ em = 620 nm was quenched. The fluorescence intensity ratio (F 620 /F 488 ) was negatively linearly correlated to S 2− concentrations in the range of 1–20 μM, with a detection limit of 0.3 μM. Furthermore, to achieve portable detection, we mixed Si/CdTe NPs with sodium carboxymethyl cellulose to prepare effective visual fluorescent sensing paper chips, which exhibited ideal porous structure, good particle dispersion, and excellent fluorescence properties. Incubating the paper chips with high-protein foods allowed for accurate monitoring of food freshness during storage. Therefore, this approach provided a reliable and portable method to determine H 2 S concentration using a novel concept to ensure the freshness and safety of high-protein foods.
研究领域:
食品科学
·
分析化学
·
材料科学
·
生物技术
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Ting Zhang,Miao Tang,SiYi Yang,Huanbao Fa,Yongzhong Wang,Danqun Huo,Changjun Hou,Mei Yang
Given the significant and potential fatal implications of glyphosate (GLY) residues on human health and the integrity of ecosystems, their presence has garnered substantial global concern and scrutiny. Herein, we introduced a pioneering colorimetric sensing platform, the first of its kind, based on ternary metal-organic frameworks (ZnCo-ZIFs@MIL-101(Fe)). This innovative platform enabled ultra-sensitive, affordable, portable and rapid on-site detection of GLY. This platform achieved a wider linear range for GLY of 0.02–40 μg/mL with an exhibiting remarkable detection limit of 1 ng/mL, which was attributed to the electronic hybridization of the Fe 3+ , Co 2+ , and Zn 2+ metal centers of ZnCo-ZIFs@MIL-101(Fe), significantly enhancing the composite's catalytic performance. The assay was successfully employed to detect GLY in food and herb samples. Moreover, to meet the demand of in-field detection for GLY, a smartphone detection method based on ZnCo-ZIFs@MIL-101(Fe) with visual, intelligent, and portable features was fabricated. This detection concentration range of GLY was 0–1 μg/mL, and the limit of smartphone detection was 23 ng/mL. Furthermore, this sensor seamlessly integrated with smartphones and paper-based microfluidic chips (μPADs), which constructed a portable test strips-smartphone sensing platform for facilitating real-time and on-site visual quantitative detection of GLY. The detection concentration range was 0–1 μg/mL, and the limit was calculated as low as 75 ng/mL. The assay was highly adaptable in practical applications. In summary, our study paved a novel pathway for the design and utilization of multi-metal MOF nanozymes in on-site pesticide monitoring.
研究领域:
分析化学
·
环境科学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2016
·Vol. 40
·期 3
·789-797
·
IF 2.741
Liangfei Ouyang,Qian Liu,Heng Liang
A common drawback of paper-based separation devices is their poor detection limit. In this study, we combined field-amplified sample stacking with moving reaction boundary electrophoresis on a paper chip with six array channels for the parallel separation and concentration of multiple samples. With a new hyphenated technique, the brown I 2 from the Fe 3+ /I − oxidation–reduction reaction emerged near the boundary between the dilute ethylene diamine tetraacetic acid and potassium iodide and highly concentrated KCl solutions. For the separation and concentration of three components, Cr 3+ , Cu 2+ , and Fe 3+ , the Fe 3+ detection limit was improved at least 266-fold by comparing the hyphenated technique with moving reaction boundary electrophoresis. The detection limit of Fe 3+ was found to be as low as 0.34 ng (20 μM) on the paper chip. We also demonstrated the analysis of a real sample of four metal ions, with detection limits as follows: 0.16 μg Cr 3+ , 1.5 μg Ni 2+ , 0.64 μg Cu 2+ , and 1.5 μg Co 2+ . The synergy of field-amplified sample stacking and moving reaction boundary electrophoresis in the micron paper-based array channels dramatically improved the detection limit and throughput of paper-based electrophoresis.
研究领域:
分析化学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Yi Wu,Liyuan Zhang,Dongjie Zhang,Runzhong Yu
A microfluidic paper chip colorimetric detection system based on surface molecular imprinting of zinc ferrite nanoparticles was established, and the detection images were obtained by smartphone for gray value analysis and determination of butachlor. The best functional monomers and addition ratio were selected by quantum chemical simulation calculation, the properties of the prepared molecularly imprinted polymers were analyzed, and the detection conditions were optimized. The linear range, sensitivity, and selectivity of the method were evaluated. The results showed that under the optimum conditions, the concentration of 2–80 ng/g had a good linear relationship (R 2 is 0.9953), the detection limit was 1.43 ng/g, the specificity was good, and the whole detection process did not exceed 20 min. The microfluidic paper chip was applied to detect butachlor in mung bean samples. The results showed that the recovery was 93.4–106.4 %, and the relative standard deviation was less than 5.6 %.
研究领域:
分析化学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）