RSC Advances
·
2015
·Vol. 5
·期 96
·78833-78840
·
IF 3.84
Lin Luo,Jin-Yi Yang,Zhi-Li Xiao,Dao-Ping Zeng,Yong-Jun Li,Yu-Dong Shen,Yuan-Ming Sun,Hong-Tao Lei,Hong Wang,Zhen-Lin Xu
Histamine (HA) is an essential test item for fishery samples. However, the fast and effective determination of HA is difficult due to its simple structure and small molecular size. In this study, a sensitive and specific monoclonal antibody against p-nitrobenzoylated histamine (NPHA), which can be easily obtained from the reaction of HA and p-nitrobenzoic acid N-hydroxysuccinimide ester (PNBA-OSu) under mild conditions, was generated for the first time. Based on this mAb, an immunochromato-graphic assay strip (ICA strip) using a colloidal gold nano particles-antibody (GNPs) probe for rapid detection of HA in saury samples was established. After screening the coating antigens and optimization of analytical parameters, a heterologous coating based ICA strip exhibited the most excellent detection ability with a visual detection limit (VDL) of 6.0 mg kg−1 in qualitative experiments and a detection limit (by a strip reader) of 1.0 mg kg−1 in semi-quantitative experiments for saury samples, with no cross-reactivity with HA analogs. Good correlation between the ICA strip with liquid chromatography-tandem mass spectrometry was achieved for spiked and naturally contaminated saury samples. Overall, this method is suitable for screening of HA residue for large scale fish samples in a quick, simple and low-cost manner.
研究领域:
分析化学
·
食品安全
·
免疫学
·
分子诊断
·
兽药残留检测
✓ 已验证
DOI
高影响因子
Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy
·
2019
·Vol. 29
·期 1
·46-53
·
IF 1.754
Peng Wei,Han Ping,Yu Luyao,Chen Ying,Ye Bingzhu,Qin Luping,Xin Hailiang,Han Ting
The fruits of Xanthium strumarium L., Asteraceae, have been used for various diseases in Chinese folk medicine, including allergic rhinitis, tympanitis, arthritis, ozena etc. The current study was aimed to investigate the therapeutic effects of caffeoylquinic acids from fruits of X. strumarium on allergic rhinitis in animals. The toxicity test indicated that the caffeoylquinic acids have no obvious toxicity. By using HPLC assays combined with reference standards, ten caffeoylquinic acids were identified as the predominant constituents. Anti-allergic activities of the caffeoylquinic acids were evaluated using passive cutaneous anaphylaxis test and Schultz-Dale test; dimethylbenzene induced ear edema test was performed to evaluate its anti-inflammatory effect. Then, the allergic rhinitis model in rats was established to evaluate the therapeutic effects of the caffeoylquinic acids against allergic rhinitis with the following indexes: allergic rhinitis symptom scores, serum levels of pro-inflammatory cytokines, histopathological examination, and histamine release. Our study revealed that the caffeoylquinic acids showed obvious anti-allergic and anti-inflammatory properties, and its treatments were beneficial for ameliorating the nasal symptoms, decreasing pro-inflammatory cytokines, and inhibiting the releases of histamine. Collectively, the caffeoylquinic acids might be utilized as effective and safe disease therapeutic agents for allergic rhinitis.
研究领域:
药理学
·
免疫学
·
过敏
·
植物化学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Zuyao Fu,Lingfeng Yang,Yuanyuan Zhang,Xuanjun Zhang,Jing Xie,Zhaoyang Ding
HRP was covalently immobilized on MAF to form a biocomposite with high activity and fluorescence. • HRP@MAF exhibited a remarkable fluorescence quantum yield of 97.77%. • HRP@MAF showed superior substrate affinity with a Km value 25-fold lower than HRP. • A dual-mode colorimetric/ratiometric fluorescent sensor was developed for histamine. Integrating strong fluorescence and efficient biocatalysis within a single metal-organic framework (MOF) is attractive for advanced optical sensing. Herein, a highly emissive metal-organic framework (denoted as MAF) was constructed using ligands with aggregation-induced emission (AIE) properties, followed by horseradish peroxidase (HRP) immobilization to yield HRP@MAF with high catalytic activity and strong fluorescence. The resulting HRP@MAF exhibited a fluorescence quantum yield of 97.77%. Benefiting from the high surface area and porous structure of MAF, HRP@MAF showed markedly improved stability and substrate affinity toward 3,3′,5,5′-tetramethylbenzidine (TMB), with a K m value 25-fold lower than that of free HRP. Based on its catalytic and optical properties, a colorimetric/ratiometric fluorescence sensor was developed for histamine detection with detection limits of 1.71 μM and 0.56 μM, respectively. In addition, smartphone-integrated on-site detection was achieved with linear ranges of 10–1000 μM for the colorimetric mode and 1–1000 μM for the ratiometric fluorescence mode. This work establishes an effective strategy for constructing multifunctional sensing platforms by coupling AIEgens derived highly emissive MOFs with immobilized enzymes. Download: Download high-res image (151KB) Download: Download full-size image
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2026
·
IF 6.7
Yu Liu,Qizheng Bi,Xiaoning Chu,Zhihao Dang,Junmin Hui,Zhuo Liu,Congkui Tian
The detection of leucine aminopeptidase (LAP) activity is essential for diagnosing liver cancer and liver injury. This study presents a novel fluorescence-based method designed for the sensitive detection of LAP through cascade catalytic reactions. Leucine-histidine (LeH) was synthesized using histidine and leucine as substrates for LAP. Notably, histidine (His) effectively inhibited the Cu 2+ -catalyzed oxidation of o-phenylenediamine (OPD) to fluorescent 2,3-diaminophenazine (oxOPD), resulting in a diminished fluorescence signal. Given that LAP hydrolyzes LeH to release His, which subsequently inhibits the Cu 2+ -OPD system, LAP activity can be quantitatively assessed by measuring fluorescence changes during the hydrolysis process. The exceptional performance of the Cu 2+ -OPD system in quantifying His allowed for highly sensitive LAP detection, achieving a detection limit as low as 0.05 U/L. Furthermore, the strategy was successfully implemented to evaluate LAP activity in cells.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Food Science & Technology
·
2026
·
IF 3
Jiayi Chen,Dandan Zhang,Lu Liu,Xiaoli Wu,Meihan Guo,Hong Yan Zou,Xi Yang
Herein, a cost-effective, naked-eye-readable platform for on-site biogenic amines (BAs) detection and shrimp freshness monitoring was developed based on a ratiometric fluorescent CDs–RhB hybrid nanosystem that was constructed by integrating carbon dots (CDs) with rhodamine B (RhB). Biogenic amines induced a significant enhancement in the green fluorescence emission of CDs, while the red fluorescence emission of RhB remained unchanged, enabling an instant self-calibration during detection. Loaded on filter paper to form disposable test strips, the nanosystem enabled a visible color transition from orange to green with increasing BAs concentration. Readable via a smartphone RGB app, the paper-based assay showed a linear response of 5.0–250 μM with a limit of detection (LOD) of 3.56 μM using histamine as the model analyte. Validated through on-site shrimp quality evaluation, the paper-based sensor established a reliable “nanoprobe–paper–phone” strategy, facilitating on-site and quantitative freshness assessment in real-world demand scenarios.
✓ 已验证
产品关联
DOI
高影响因子
CHIRALITY
·
2026
·Vol. 38
·期 3
·e70091
·
IF 2.4
Mengwei Zhang,Shuyi Song,Yingao Wang,Songze Wang,Mingshan Zheng,Xi-Ling Li,Jun Zhe Min
Doenjang is the most used fermented soy product in Asian countries. At present, the simultaneous determination of DL-amino acids and histamine in doenjang has not been reported. Therefore, an UHPLC–MS/MS method was developed based on a novel chiral derivatization probe DBD-M-Pro, enabling for the first time the simultaneous quantification of 10 DL-amino acids and histamine in doenjang samples from different regions. Achieving resolution (Rs) values of 1.62–6.31 for the 10 DL-amino acids. The limits of detection (LODs) for DL-amino acids and histamine ranged from 0.54 to 15.62 pmol. The intraday and interday precision (RSD) were 0.21%–10.63%, and the average recoveries were 93.18%–109.07%. This method was applied to analyze 12 doenjang samples from Northeastern China, Korea, and Japan. The results showed that there was a significant geographical distribution of DL-amino acids and histamine. Yanbian Korean doenjang from China showed a unique advantage in that the total amount of DL-amino acids was significantly higher than that from other regions, with histamine concentration generally increasing with longer fermentation times. This study provides a stable and reliable method for the simultaneous determination of chiral amino acids and histamine in fermented foods such as doenjang.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Microbiology Spectrum
·
2025
·
IF 3.8
Fan Yang,Wei Dai,Hua Xue,Wen Chen,Chen Liu,Yaru Tian,Wanli Cheng,Jibin Zhang
Root-knot nematodes (RKNs) are notoriously challenging to control because of their broad host spectrum and swift proliferation. Consequently, there is an urgent need to develop safe and effective nematicides. Microorganisms and their metabolites have the potential to control RKNs safely and effectively. Paenibacillus polymyxa KM2501-1 can produce a range of volatile organic compounds (VOCs) with nematicidal activities, among which 2-undecanol is a potential biocontrol agent against Meloidogyne incognita. Nonetheless, the mechanism by which 2-undecanol prevents RKN infection in plant roots remains unclear. 2-Undecanol has many activities against M. incognita in vitro, including contact (LC50 = 34.5 mg/L), fumigation (LC50 = 191.6 mg/L), attraction, locomotion inhibition (frequency of head thrash and body bend reduced by 84.0% and 97.0% at concentration of 40 mg/L) and egg hatching inhibition (by 98.5% at concentration of 80 mg/L). Upon treatment with 2-undecanol, a noticeable reduction in the number of galls was observed, and the control efficacy reached 60.8% at the dose of 5 mg/pot. Metabolomics analysis revealed that the application of 2-undecanol significantly influenced the composition of root exudates of tomato. Among the 17 differential metabolites, the up-regulated 10-undecenal could attract and kill M. incognita J2 larvae at a concentration of 100 mg/L, and cyclohexylamine had nematicidal activity at the concentration of 1,000 mg/L. This study revealed the mechanism of 2-undecanol inducing plant roots to secrete nematicidal substances with attractant activity, effectively controlling RKNs, which could pave the way for the development of environmentally friendly and cost-effective nematicides.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FITOTERAPIA
·
2026
·
IF 2.9
Huarun Li,Shiyu Wen,Xiangyue Zhao,Xiuxian Zhou,Xianwen Li,Hongping Xiang,Lanyue Zhang,Si Qin
Traditional Chinese medicine Glycyrrhiza uralensis ( G. uralensis ) exerts anti-inflammatory and anti-allergic effects. However, there is currently no valid evidence regarding its anti-histamine effect. This study aimed to elucidate the antihistamine effect of Glycyrrhiza uralensis Fisch essential oils (GUEOs) and glabridin (Gla) extracted from G. uralensis in SPF KM mice and investigate the underlying mechanisms. The chemical components of GUEOs were analyzed using gas chromatography–mass spectrometry (GC–MS), and quality control was conducted. An acute histamine allergy model was established in SPF KM mice following one week of continuous histamine administration. Scratching behavior and appearance of back scratches were observed. Hematoxylin and eosin staining was used to detect the thickness of the mouse dermis. Toluidine blue staining was performed on dermal tissues to count the number of mast cells. Immunohistochemistry was used to determine the integrated optical density (IOD) values of nerve growth factor (NGF) and interleukin-1β (IL-1β). Quantitative Real-time polymerase chain reaction (qRT-PCR) was used to determine the relative intensity of IL-4 and IL-6. Enzyme-linked immunosorbent assay (ELISA) was employed to detect the content of interferon-γ (IFN-γ)，IL-1 and filaggrin (FLG). Compared with the model group, both the GUEOs and Gla groups exhibited reduced scratching behavior in mice, accompanied by epidermal thinning, decreased mast cell numbers, downregulated expression of IL-1β, NGF, IL-4, IL-6, IFN-γ, and IL-1, and upregulated FLG expression. We showed that GUEOs and Gla exert a favorable inhibitory effect on histamine-induced allergic inflammation in mice, suggesting that G. uralensis can serve as a promising natural anti-histamine agent.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.1
Yidan Zhang,Yuhua Lai,Yifei Ma,Lin Ge,Jinhua Liu
Since excess histamine (His) in seafood poses a significant health threat, the development of intelligent, on-site, and visual multimodal methods for detecting fish freshness has attracted widespread attention. In this study, we developed a Lewis base-induced paper-based chip coupled with smartphone RGB (Red Green Blue) recognition for the real-time colorimetric/fluorescent dual-mode detection of His in fish meat. The sensing mechanism was based on the catalytic role of His in rapidly forming a green fluorescent compound (GFC) from dopamine and 3-ethylamino-4-methylphenol. Interestingly, the introduction of 1% Tween (TW) 80 was found to significantly enhance the fluorescence signal and real-time stability of the system, achieving an exceptional detection limit of 16.67 nM for His. By immobilizing the GFC probe on Whatman 1 filter paper, a paper-based test strip for His was further developed. His quantification is achieved through RGB analysis with a smartphone, allowing visual and real-time assessment of fish meat freshness directly in the field. The developed sensor exhibits outstanding selectivity, stability, and sensitivity, and has been successfully applied to monitor His levels in fish meat samples (crucian, saury and golden pomfret) over time, providing a reliable means to prevent His poisoning. Therefore, this work presents a robust, low-cost, and user-friendly point-of-care testing platform that holds significant potential for applications in intelligent food monitoring and environmental sensing.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2026
·
IF 4.1
Wei Xiaolan,Wang Peng,Li Linjin,Luo Zhu,Ye Xinyuan,Long Jiujiang,Liu Xianghui
Biogenic amines (BAs), primarily formed through the microbial degradation of proteins, are important markers indicating food fermentation and spoilage. On the demand of on-site monitoring of food quality and safety, electrochemical and optical sensors have been developed, focusing on the detection of the total volatile amines or a specific amine. However, the multiplex detection of BAs with high accuracy and sensitivity still remains a major challenge. The sensor array strategy that mimics the biological olfactory sense provides an effective and powerful route to achieve multiplex detection of BAs. Herein, (metallo)porphyrin-based hydrogen-bonded organic frameworks embedded with silver nanoparticles were prepared and used as receptors to construct the surface-enhanced Raman scattering (SERS) sensor array, leveraging the multiple chemical interactions and the sharp Raman signals of (metallo)porphyrins, and the porous and flexible structure of the supramolecular frameworks. This SERS sensor array demonstrated a successful discrimination of ten BAs, an accurate quantitation of three BAs, and a successful simultaneous determination of three BAs in pork samples. Using the total volatile basic nitrogen as a spoilage label, the platform achieved over 90% accuracies in freshness discriminations for pork, beef, and chicken samples. In comparison with the existing detection methods, the proposed SERS sensor array strategy based on porphyrin- and metalloporphyrin-based hydrogen-bonded organic frameworks exhibits operational simplicity, rapid response, high sensitivity, as well as multiplex detection capability. Thus, it demonstrates a significant practical potential for applications in the production line and the supply chain of foods.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2023
·
IF 4.3
Baihui Wang,Huan Jiang,Ruyi Tang,Yaoyu Tan,Xuemin Xia,Xia Zhang
The activity of nanozymes is dependent on their surface for catalysis, and bioconjugation or adsorption of DNA aptamers will promote their activity. This paper aims to develop a novel aptamer sensor, for rapid, sensitive and specific detection of histamine. The design of the aptamer biosensor is based on the adsorption of histamine aptamers onto the surface of Au NPs by bioconjugation, which promotes the peroxidase activity of the nanozymes. Since histamine is strongly selective and specific for histamine aptamers, the histamine aptamer can be detached from the surface of Au NPs and bound to histamine, during which the catalytic activity of the nanozymes falls back due to the detachment of the histamine aptamer, which can be quantitative analysis by the TMB-H 2 O 2 system. The decrease in enzyme catalytic activity became larger with increasing histamine concentration, with a good linear correlation. Finally, the histamine aptamer biosensor was constructed, which has the advantages of ultrasensitive, high-accuracy, and wide linear range. The linear range and detection limit are 10 −11 ∼10 −3 M and 1.22 × 10 −12 M, respectively. With reference to this aptamer sensor design, this approach can also be extended to other schemes where the aptamer is combined with Au NPs as a way to detect the corresponding molecules.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Water Process Engineering
·
2023
·
IF 7
Ruixue Wang,Zhongwen Li,Xiaosen Li,Peiwen Guo,Haibo Wang,Xuan Guo,Jinyi Zhong
Atmospheric pressure plasma jet (APPJ), as an emerging clean and efficient technology, has been found a great potential for chemical warfare agents (CWAs) decontamination. In this study, we first proposed APPJ for the degradation of sulfur mustard (HD) and its surrogate 2-chloroethyl ethyl sulfide (2-CEES) in water. Under optimal conditions, the degradation efficiency of 2-CEES and HD reached 100 % after 5 min or 6 min plasma treatment. The degradation products of 2-CEES and HD were mainly non-toxic alcohols or low toxic sulfones, which can be degraded and converted into some small molecules (HCl, SO 2 , or CO 2 ) under further plasma treatment. We then identified two dominant short-lived reactive species (·OH and 1 O 2 ) and long-lived reactive molecule (H 2 O 2 ) and distinguished the effect of these three active particles during the degradation process of 2-CEES in water. This work provides detailed evidence of a novel decontamination strategy for HD and 2-CEES.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）