ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Muhammad Saqib,Wen-Xin Chen,Yun Lei,Ai-Lin Liu
Chemiluminescence (CL) assays are often constrained by the limited availability of coreactants that combine strong signal amplification with biocompatibility. Herein, we report for the first time that sericin, a natural protein derived from silk, serves as a highly efficient coreactant in the lucigenin CL system under alkaline conditions. Impressively, this new lucigenin–sericin CL system generates an unprecedented ∼43-fold higher emission intensity compared to the traditional lucigenin–hydrogen peroxide system. Moreover, the mechanistic studies reveal that sericin, with its electron-rich functional groups, significantly promotes the generation of superoxide radicals (O2•–). This, in turn, accelerates the lucigenin oxidation pathway, leading to significant enhancement in CL signal. Leveraging this enhanced system, we developed a dual-function CL platform for the ultrasensitive detection of sericin (limit of detection, LOD = 0.12 μM) and superoxide dismutase (SOD, LOD = 0.28 ng/mL), exhibiting an extended linear range spanning from 0.25 to 50 μM and 0.5 to 60 ng/mL, respectively, the latter based on CL quenching phenomena. This method offers compelling superiority in terms of simplicity, rapid analysis, enhanced sensitivity, higher selectivity, and cost-effectiveness. Moreover, this method successfully achieved the detection of sericin in cosmetics and the SOD in human serum samples. Importantly, this work represents the first chemiluminescent method for sericin detection. In addition, the system exhibited high sustainability and practical utility, as confirmed by AGREE (greenness) and BAGI (blueness) assessment tools. Furthermore, it introduces a new class of protein-based coreactants for CL systems, expanding the frontier of (bio)analytical detection.
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BIOMEDICAL CHROMATOGRAPHY
·
2026
·Vol. 40
·期 3
·e70382
·
IF 1.7
Linghao Zhao,Wei Yang,Shengjin Cui,Jingang Luo,Yaxin Cao,Nan Yan,Hongying Wang
Interference is a significant source of laboratory errors that can cause erroneous measurement results. In clinical practice, all the measured results should exclude the influence of interference. In this study, the ion exchange high-performance liquid chromatography of hemoglobin A1c (HbA1c) was evaluated via three brands of HbA1c analyzers, and 12 endogenous and 24 exogenous substances were added to hemoglobin solutions with different HbA1c levels to simulate the corresponding clinical samples. After the potential interfering substances were screened via interference screening testing, the interference concentration thresholds of each potential interfering substance were determined on the basis of dose–response experiment. When these substances exceed the interference concentration thresholds, elevated HbA1c values are caused by endogenous cholic acid and low pH, whereas exogenous ethanol increases HbA1c measurements in samples with low values. However, HbA1c measurements decreased in samples with high values. Although there are some differences in the thresholds of interferent concentrations between different brands of analyzers, the differences are not significant. On the basis of the detailed interference concentration thresholds and interference substances, this evidence can be provided to clinical laboratory experts and help them interpret and determine the testing results of clinical laboratories.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.6
Lian-Lian Duan,Wen-Jun Liu,Rui Zhai,Zhen-Guang Wang,Hong-Yuan Yan,Yun-Kai Lv
This study employed a pore-confined synthesis strategy to achieve the in situ growth of bovine serum albumin-capped copper nanoclusters (BSA-CuNCs) within the UiO-66 framework (BSA-CuNCs@UiO-66). This nanocomposite enables highly sensitive and specific detection of creatinine (CR). Results demonstrated that the spatial confinement imposed by UiO-66 induced aggregation of the BSA-CuNCs and suppressed non-radiative transitions, leading to an approximately 10-fold enhancement in fluorescence intensity and a 11-fold increase in quantum yield. Leveraging the specific adsorption and enrichment capability of the UiO-66 framework toward CR, the BSA-CuNCs@UiO-66 fluorescence probe exhibited significant fluorescence quenching upon exposure to CR, achieving a detection range of 50–1000 nM and a detection limit of 30.81 nM. This work presented a novel confinement engineering strategy utilizing metal-organic frameworks (MOFs), establishing a new design paradigm for high-performance fluorescence probes with significant potential in bioanalytical applications.
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Nature Communications
·
2026
·
IF 15.7
Chen Yue,Chen Qinyi,Ma Yuanyuan,Zhang Cheng,Xu Jie,Li Kejing,Sun Yajie,Su Xinge,Wei Mingguang,Bao Rui,Ding Tong,Tang Longguang,Yu Wenying,Ju Shenghong,Fan Wenpei
Radiotherapy (RT) is a clinical mainstay of cancer treatment that triggers tumor-specific immune responses. However, the effectiveness is usually hampered due to the hypoxic tumor microenvironment (TME) and the ambivalent impact of RT on the immune landscape of tumors. Herein, we develop an injectable hydrogel encapsulating interleukin-12 (IL-12)/anti-CTLA-4 (aCTLA-4) co-engineered red blood cells (RBC), which is in situ self-assembled within the TME to increase oxygen supply and instigate sequential aCTLA-4/IL-12 release, thus achieving Ba/O 2 self-compensated radiosensitization and activating multistage immune responses. Once in the acidic TME, the in situ injected BaO 2 undergoes hydrolysis to generate H 2 O 2 and Ba 2+ , followed by the rapid reaction of Ba 2+ with sodium alginate to afford a biocompatible hydrogel. Meanwhile, catalase presented on RBC converts H 2 O 2 into O 2 , thereby alleviating hypoxia-induced radioresistance and inducing O 2 -mediated pore formation on RBC membrane for rapid release of aCTLA-4 to relieve tumor immunosuppression. Subsequently, IL-12 anchored on RBC is dilatorily released and interacts with T/NK cells within the TME to induce IFN-γ-dependent antitumor immunity. Taken together, the in situ self-assembled cell reservoir hydrogel offers a futuristic avenue to realize multistage radioimmunotherapy for effective tumor regression by programmable immunoregulation with significant clinical value.
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2026
·
IF 5.6
Dechan Lu,Xiaoqi Zhou,Dandan Su,Jundong Li,Huizhen Lin,Rongmao Qiu
Abnormal serum creatinine levels are a key clinical biomarker for kidney dysfunction, making its accurate monitoring essential for early diagnosis and prevention. Current commercial kits based on the creatine kinase method are often affected by matrix interference, limiting their reliability in clinical settings. To address this issue, we developed a pH- assisted ratiometric surface-enhanced Raman scattering (SERS) approach for precise creatinine detection in biological samples. We synthesized core-shell nanoparticles (Au@MPA@Ag NPs) with 3-mercaptopropionic acid (MPA) as the internal standard. Under optimized alkaline conditions (pH = 9), the adsorption of creatinine onto the substrate was significantly enhanced, leading to a strong and reproducible SERS response. Moreover, the internal standard-calibrated Raman intensity exhibited a strong linear correlation with creatinine concentration, achieving the limit of detection as 3.3 μM. As a proof of concept, this method was successfully applied to quantify creatinine in human serum. The recovery rates ranged from 98% to 107%, demonstrating high consistency with results obtained using a commercial creatinine assay kit. This method provides a clinically accurate and reliable for creatinine monitoring, offering strong potential for point-of-care testing.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2026
·
IF 4.1
Liu Feng,Yu Decong,He Kun,Song Yanan,Li Yichao,Gao Haoxuan,Wu Bing,Li Xiaoyu,Zhao Ji,Xu Fei,Yang Huifang
The widespread use of neonicotinoids has raised growing concerns about their potential health risks. In this study, we developed a robust and high-throughput analytical method based on liquid-liquid extraction (LLE) and dispersive solid-phase extraction (dSPE), followed by high-performance liquid chromatography-quadrupole-Orbitrap high-resolution mass spectrometry (HPLC-Q-Orbitrap HRMS) for the simultaneous determination of 11 neonicotinoids and their 6 metabolites in human urine. After pretreatment of LLE and dSPE, qualitative and confirmatory analysis was achieved using tMS 2 acquisition of HPLC-Q-Orbitrap HRMS, and quantitative analysis was performed using isotope-labeled internal standards and matrix-matched calibration curves. The method was validated with good linearity ( R 2 > 0.9975), recoveries of 75.8%~118.9% (RSDs < 15%), and detection limits of 0.001~0.06 μg/L. The established method was successfully applied to urine samples of community-dwelling elderly population individuals ( n = 150) and to a suspected case of acute acetamiprid poisoning in Ningxia, China. The results showed that thirteen neonicotinoids and metabolites were detected in the elderly cohort, and the poisoning incident was confirmed to be caused by acetamiprid and its metabolite N-desmethyl-acetamiprid. Meanwhile, Spearman correlation analysis indicated significant positive correlations between parent compounds and their metabolites for all analytes except acetamiprid, and a strong correlation between clothianidin and thiamethoxam, suggesting shared exposure sources or common metabolic pathways. The established method is sensitive, accurate, and high-throughput, and has proven reliable for human biomonitoring and health risk assessment of neonicotinoid exposure. Graphical
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.8
Xueer Lin,Jiahui Zou,Guancheng Zhu,Linfeng Pang,Yuqi Chen,Yanyan Huang
Heterocyclic aromatic amines (HAAs), formed during the thermal processing of meat, are a group of carcinogenic and mutagenic compounds that pose significant risks to food safety and human health. Among them, 2-amino-3-methylimidazo[4,5- f ]quinoline (IQ) is considered one of the most hazardous substances, making its monitoring critically important. However, conventional detection techniques are often limited by sophisticated and costly instruments, complex operation process, and highly trained personnel. In this study, we proposed a “turn-on” colorimetric sensing strategy for the detection of IQ based on the analyte-triggered enhancement of oxidase-like activity of Ce-UiO-66 nanozyme. Ce-UiO-66 was synthesized using a simple solvothermal method via the self-assembly of terephthalic acid and Ce 4+ ions. The resulting Ce-UiO-66 exhibited excellent oxidase-like activity, catalyzing the oxidation of 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) via the generation of superoxide anions from oxygen. In the presence of IQ, electrostatic interactions facilitated the rapid adsorption of IQ onto the negatively charged Ce-UiO-66 surface, reversing the surface charge to positive. This charge shift enhanced the affinity of Ce-UiO-66 for negatively charged ABTS, significantly accelerating its oxidation. Based on this principle, we established a “turn-on” colorimetric sensing strategy for the detection of IQ, achieving a detection limit of 5.41 μM. This Ce-UiO-66 nanozyme-based detection method demonstrated high selectivity and anti-interference performance and could efficiently distinguish its analog 2-amino-1-methyl-6-phenylimidazo[4,5- b ]pyridine (PhIP) in complex food matrices. Further experimental data illustrated that this strategy was successfully applied to the detection of IQ in roasted meat samples. This work might offer a simple, cost-effective, and robust approach for rapid screening of IQ in highly contaminated processed meats.
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ACS Applied Materials & Interfaces
·
2026
·
IF 7.8
Dongxue Feng,Yuxin Zhou,Yao Tong,Jian Yang,Jinlou Gu
The assembly of covalent organic frameworks (COFs) in enzyme-compatible media is highly desirable yet challenging. Herein, we proposed an electron donor–acceptor (EDA)-complex-assisted strategy for the aqueous ambient construction of β-ketoenamine COFs under additive-free condition simultaneously with the in situ encapsulation of enzymes. EDA complexes, formed by an electron-rich 2,2′-bipyridine-5,5′-diamine (Bpy) with electron-deficient 1,3,5-triformylphloroglucinol (Tp), prearranged monomers in specific orientation and significantly lowered the reaction activation energy, thus enhancing the error correction and yielding highly crystalline TpBpy COFs. Beyond affording a protective scaffold, the Bpy units within COFs also served as anchoring sites for the chelation of various metallic ions to establish biomimetic enzymatic centers. On this basis, a spatially coupled nanoreactor was designed by encapsulating the natural creatinine deiminase (CD) followed by biomimetic laccase (Bpy-Cu) installation within COFs. The product generated by the encapsulated CD activated the neighboring Bpy-Cu within the adjacent pores, creating a proximity-induced cascade amplification and enabling the entire cascade to respond specifically to one of the key chronic kidney disease (CKD) biomarkers of creatinine. The constructed detection platform exhibits a wide linear range, with a detection limit as low as approximately 7.7 μM. This platform can provide valuable supporting information for the assessment of kidney function and holds promise for applications in the early screening and disease course monitoring of CKD. By simply altering the encapsulated natural enzymes and biomimetic active sites, this modular and generalizable platform is expected to offer a promising tool for early diagnosis of various diseases.
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ANALYTICA CHIMICA ACTA
·
2026
·
IF 6.1
Haiyan Cao,Bin Yang,Wenfei Dong,Wenbing Shi
Background The accurate detection of 2,3,5,6-tetramethylpyrazine (TMP) in complex matrices is significant for food quality control and biomedical analysis. Despite this need, a dedicated optical sensing platform for TMP has not been reported to date. Results Here, we present a novel ratiometric fluorescent probe based on a Tb–EDTA complex, which introduces a sensing strategy founded on coordination-induced modulation of the ligand-to-metal charge transfer (LMCT) process. Specific coordination of TMP's pyrazine nitrogen atoms to the Tb 3+ center results in a characteristic ratiometric response: quenching of the emission at 548 nm and concomitant enhancement at 650 nm. The probe exhibits excellent analytical performance with a linear range of 0.1―40 μM, a detection limit of 0.038 μM, and high selectivity over structurally similar interferents. Its practical applicability is demonstrated by reliable quantification of TMP in real samples, including sauce-flavor Baijiu, sake, and human urine, with recoveries of 94.54–104.82% and relative standard deviations below 10.57%. Significance This work establishes a pioneering optical sensing approach for TMP detection, providing a valuable analytical tool for flavor assessment in alcoholic beverages and pharmacological monitoring in biological systems.
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Polymers
·
2026
·Vol. 18
·期 13
·1647
·
IF 5.8
Zhongqiu Ge,Hengfeng Zhu,Yiyang Chen,Yihang Rong,Zhuqun Shi,Quanling Yang
Most adsorbent materials typically face difficulties such as poor blood compatibility, weak mechanical strength, and high cost. In this study, oxidized 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) was used to obtain cellulose nanofiber (TOCN), and cellulose beads were prepared using a drop curing method. The structure, adsorption properties, and blood compatibility of the prepared beads were thoroughly investigated. The TOCN beads exhibit a uniform, nanometer-scale, three-dimensional porous structure. With increasing carboxyl content, after adsorption of TOCN beads, the bilirubin concentration in rabbit plasma decreased from 0.03 to 0.0089 mg mL−1within 90 min, which is significantly lower than the average bilirubin concentration in humans (about 0.01 mg mL−1), and the bilirubin concentration decreased by about 70%. The results illustrated the excellent blood compatibility, self-anticoagulant ability, and superior toxin removal capabilities of the TOCN beads, highlighting their potential as an ideal blood purification adsorbent.
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Soft Science
·
2026
·Vol. 6
·期 2
·N
·
IF 15.9
Jiang Cheng,Naihui Hou,Tengda Wang,Zhenyun Zhao,Wei Chen
With the growing global burden of chronic stress, there is an increasing need for real-time, non-invasive monitoring of cortisol dynamics. Molecularly imprinted polymer (MIP)-based sensing technology is known for its cost-effectiveness and low susceptibility to deactivation. Current invasive MIP-based cortisol sensors face fundamental requirements for improving sensitivity and widening the detection range. In addition, they encounter challenges in improving wearability and portability, as well as in developing commercially viable techniques. This work designs dual-function core-shell nickel hexacyanoferrate-MIP nanocubes (NiHCF-MIP NCs) and accordingly proposes a one-step conjugate electrospinning technique to yield coaxial cortisol-sensing yarns. The core-shell architecture effectively integrates the redox signal transduction capability of NiHCF with the cortisol-specific recognition function of MIP, enabling a continuous one-step fabrication process. The utilization of conjugate electrospinning technology not only supports scalable manufacturing but also creates a coaxial yarn structure that combines the advantages of the spun nanofiber network cortex and core threads. The as-produced yarns simultaneously possess high conductivity, flexibility, wearability, and rapid body fluid absorption, collectively enhancing the sensing performance. Consequently, the electrospun yarns exhibit a high sensitivity of 2.08 μA·dec-1, and the limit of detection (LOD) is theoretically calculated to be 0.4 nmol/L. This synergistic strategy combines core-shell dual-function nanomaterials with a one-step continuous conjugate electrospinning technique. It provides an innovative pathway for developing integrated wearable biosensing textiles for personal real-time stress monitoring and telehealth applications.
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TALANTA
·
2026
·
IF 6.7
Hui Kong,Chengchen Tang,Daiyue Zhang,Kexin Chen,Yinhua Liu,Lianqun Zhou
Dynamic assessment of renal function has become a critical requirement in clinical and health management. Existing detection methods primarily rely on samples such as blood or urine, posing significant challenges for portable, continuous, real-time monitoring. This study introduces a novel wearable electrochemical biosensing platform based on sweat analysis for non-invasive detection of creatinine, a key biomarker of renal function. The platform employs o-phenylenediamine as a functional monomer to construct a molecularly imprinted polymer recognition layer. Leveraging the multi-point hydrogen bonding interactions provided by its o-diaminic groups, this layer forms strong and specific bonds with creatinine, endowing the sensor with high molecular specificity and anti-interference properties. The system integrates a flexible skin-adhesive interface, an electrochemical detection unit, and a pentagonal star-shaped sweat collection module. It induces localized sweating at rest via built-in electrodes, enabling efficient transport and real-time analysis of fresh sweat. The sensor exhibits excellent linear response characteristics across a concentration range of 0.1 to 400 μM, with two linear regions, both showing correlation coefficients R 2 > 0.99. The platform demonstrated stable detection performance in authentic human sweat samples. Tests evaluating circadian variations in serum creatinine and the impact of protein intake confirmed the sensor's ability to capture fluctuations linked to dietary intake and physiological rhythms. This validates its feasibility for non-invasive, dynamic monitoring of renal function. This wearable device platform shows significant potential as a sweat biomarker monitoring tool, offering possibilities for convenient tracking and personalized management of kidney health.
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