PLANT JOURNAL
·
2018
·Vol. 94
·期 2
·288-303
·
IF 5.775
Jie Qian,Yueyue Chen,Ying Hu,Yingtian Deng,Yang Liu,Gang Li,Wenxuan Zou,Jie Zhao
Summary Replication factor C (RFC) is a conserved eukaryotic complex consisting of RFC1/2/3/4/5. It plays important roles in DNA replication and the cell cycle in yeast and fruit fly. However, it is not very clear how RFC subunits function in higher plants, except for the Arabidopsis ( At ) subunits AtRFC1 and AtRFC3 . In this study, we investigated the functions of AtRFC4 and found that loss of function of AtRFC4 led to an early sporophyte lethality that initiated as early as the elongated zygote stage, all defective embryos arrested at the two- to four-cell embryo proper stage, and the endosperm possessed six to eight free nuclei. Complementation of rfc4-1 /+ with AtRFC4 expression driven through the embryo-specific DD45pro and ABI3pro or the endosperm-specific FIS2pro could not completely restore the defective embryo or endosperm, whereas a combination of these three promoters in rfc4-1 /+ enabled the aborted ovules to develop into viable seeds. This suggests that AtRFC4 functions simultaneously in endosperm and embryo and that the proliferation of endosperm is critical for embryo maturation. Assays of DNA content in rfc4-1 /+ verified that DNA replication was disrupted in endosperm and embryo, resulting in blocked mitosis. Moreover, we observed a decreased proportion of late S-phase and M-phase cells in the rfc4-1 /– FIS 2; DD 45; ABI 3pro::At RFC 4 seedlings, suggesting that incomplete DNA replication triggered cell cycle arrest in cells of the root apical meristem. Therefore, we conclude that AtRFC4 is a crucial gene for DNA replication.
研究领域:
植物学
·
遗传学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Cell and Developmental Biology
·
2024
·
IF 4.6
Romualdo Sciorio,Luca Tramontano,Gerard Campos,Pier Francesco Greco,Giuseppe Mondrone,Anna Surbone,Ermanno Greco,Riccardo Talevi,Nicola Pluchino,Steven Fleming
Over the past 40 years there has been a worldwide critical change in the field of assisted reproduction technology (ART), leading to the increased application of single blastocyst transfer, which is extremely important to avoid the risks of multiple pregnancy and associated complications for both mother and babies. Indeed, advancements in ART over the last few decades have been obtained thanks to several improvements, including ovarian stimulation, embryo culture conditions and, of course, progress in cryopreservation methods, especially with the application of vitrification. The ability to cryopreserve human embryos has improved significantly with vitrification compared to the initially adopted slow-freezing procedures. Since the introduction of vitrification, it has become the gold standard method to effectively cryopreserve human blastocysts. However, some new protocols are now being explored, such as the short warming procedure and even shorter exposure to the equilibration solution before vitrification, which seem to provide optimal results. Therefore, the main aim of the current narrative review, will be to illustrate the benefit of vitrification as an effective method to cryopreserve the human blastocyst and to illustrate new protocols and variations which in future may increase the performance of vitrification protocols.
研究领域:
生殖医学
·
胚胎学
·
辅助生殖技术
·
低温生物学
·
临床胚胎学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2014
·
IF 3.259
Huihui Ti,Qing Li,Ruifen Zhang,Mingwei Zhang,Yuanyuan Deng,Zhencheng Wei,Jianwei Chi,Yan Zhang
This study quantified free and bound phytochemicals and their antioxidant activity in the endosperm and bran/embryo of different indica rice varieties. Phytochemicals mainly existed as free form in the bran/embryo and as both free and bound forms in the endosperm. The average values of total phenolic content, flavonoid content, FRAP, ABTS and ORAC values in the bran/embryo were 3.1, 10.4, 8.2, 11.2 and 11.4 times higher than those in the endosperm, respectively. In whole brown rice, the bran contributed 59.2%, 53.7%, 47.7%, 55.5% and 56.9% of total phenolics, flavonoids, FRAP, ABTS and ORAC values, respectively. Seven individual phenolics (gallic, protocatechuic, chlorogenic, caffeic, syringic, coumaric and ferulic acids) were detected with most coumaric and ferulic acids in the bran. All measurements exhibited varietal differences. These findings provide important information for improving human health by encouraging the consumption of whole brown rice and its use in food product development.
研究领域:
食品科学
·
植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW PHYTOLOGIST
·
2025
·
IF 8.1
Peng-Bo Wang,Yao-Yuan Duan,Ru-Meng Quan,Meng-Qi Feng,Jie Ren,Yong-Yu Tang,Mei Qing,Kai-Dong Xie,Wen-Wu Guo,Xiao-Meng Wu
Summary Somatic embryogenesis (SE) is an important in vitro regeneration approach for plants, especially in biotechnological manipulations. However, SE capability is difficult to modulate and deteriorates over time. Glycerol medium is effective in SE induction of citrus, while the mechanisms remain unclear. We found that auxin signaling reduced soon after the citrus embryogenic callus (EC) was transferred to glycerol medium, and the expression of CsTCP14 and AUX/IAA gene CsIAA4 was induced by glycerol. Overexpression of CsIAAm that encodes a stable indole-3-acetic acid (IAA) protein suppressed auxin signaling in EC and enhanced SE. CsTCP14 bound to the promoter of CsIAA4 and activate CsIAA4 expression in EC with strong SE competence. Overexpression of CsTCP14 activated CsIAA4 expression and reduced auxin signaling in citrus EC, and thus enhanced SE. Application of exogenous IAA inhibits SE, while the auxin signaling inhibitor p -chlorophenoxyisobutyric acid (PCIB) enhances SE in citrus. The SE enhancement effect of CsIAA4 and CsTCP14 overexpression on EC was alleviated by exogenous IAA, but reinforced by PCIB. We uncover the regulatory pathway of CsTCP14-CsIAA4 module-mediated repression of auxin signaling in glycerol-induced citrus SE, which deepens our understanding of SE mechanisms in plants and supports modulation of SE in citrus breeding via biotechnological approaches.
研究领域:
植物生物技术
·
分子生物学
·
植物发育
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SMALL RUMINANT RESEARCH
·
2025
·
IF 1.4
Jia Hao,Si Ouyang,Yubing Wang,Lei Cheng,Dayong Chen,Chunjuan Qiu,Riga Wu,Yongji Xie,Jianhui Tian,Lei An,Guangyin Xi
This study aims to investigate the effect of cyclic guanosine-3’,5’-monophosphate (cGMP) on ovine embryo development, thereby providing new strategies to improve the efficiency of in vitro embryo production (IVP). Following in vitro maturation (IVM) and in vitro fertilization (IVF) of ovine oocytes derived from abattoir, the presumptive zygotes were treated with different concentrations of cGMP. In order to explore the effect of cGMP on the developmental competence of embryos derived from ovum pick-up (OPU)-oocytes, the presumptive zygotes were cultured with cGMP after IVF. The cleavage rate and blastocyst rate were recorded at 24 h and on day 7 after IVF. On day 7, the blastocysts derived from slaughterhouse oocytes were collected to assess mRNA expression of development- and apoptosis-related genes. Additionally, the blastocyst quality, antioxidant capacity, mitochondrial function and lipid metabolism of blastocysts were evaluated. The results showed that cGMP significantly increased cleavage rate and blastocyst rate, with an increase in total cell number and ICM. The expression of pluripotency-related gene ( NANOG ) was upregulated and apoptosis-related gene ( CASPASE3 ) was downregulated after cGMP treatment. The administration of cGMP resulted in decreased lipid content and fatty acid levels, along with a significant decrease in reactive oxygen species (ROS) and lipid peroxidation. Additionally, mitochondrial membrane potential and ATP levels were elevated after cGMP treatment. Furthermore, cGMP also increased blastocyst rate of IVF embryos from ovine oocytes collected via OPU. These results demonstrate that cGMP can improve embryo developmental competence by enhancing lipid metabolism and reducing oxidative stress.
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Chemistry
·
2018
·
IF 4.155
Osin Oluwatomiwa A.,Yu Tianyu,Cai Xiaoming,Jiang Yue,Peng Guotao,Cheng Xiaomei,Li Ruibin,Qin Yao,Lin Sijie
The photocatalytic activity of TiO2 based photocatalysts can be improved by structural modification and elemental doping. In this study, through rational design, one type of carbon and nitrogen co-doped TiO2 (C, N-TiO2) photocatalyst with mesoporous structure was synthesized with improved photocatalytic activity in degrading 4-nitrophenol under simulated sunlight irradiation. The photocatalytic degradation efficiency of the C, N-TiO2 was much higher than the anatase TiO2 (A-TiO2) based on absorbance and HPLC analyses. Moreover, using zebrafish embryos, we showed that the intermediate degradation compounds generated by photocatalytic degradation of 4-nitrophenol had higher toxicity than the parent compound. A repeated degradation process was necessary to render complete degradation and non-toxicity to the zebrafish embryos. Our results demonstrated the importance of evaluating the photocatalytic degradation efficiency in conjunction with the toxicity assessment of the degradation compounds.
研究领域:
环境科学
·
材料科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
REPRODUCTIVE BIOMEDICINE ONLINE
·
2020
·
IF 3.218
Xiaoyan Wan,Xiaomei He,Qian Liu,Peng Duan,Honggang Li
Research question Frequent and mild scrotal heat stress (fmSHS) often occurs in humans. The aim of this study was to determine the impact of fmSHS on natural fertility , the IVF–embryo transfer (IVF–ET) process and the offspring sex ratio. Design Male mice were randomly divided into four groups: no scrotal heat stress (SHS) (controls) and those subjected to SHS at 37°C, 39°C or 41°C for 30 min once a week for 5 consecutive weeks. The testis, epididymis and sperm quality were assessed to evaluate the effects of different degrees of SHS, to establish an fmSHS model. Then, natural fertility, IVF–embryo transfer (IVF–ET) results, embryo development, offspring sex ratio and the X/Y chromosome-bearing sperm ratio were examined. Results SHS at 39°C and 41°C caused mild impairment to spermatozoa , leading to a phenotype similar to oligoasthenozoospermia in humans. Given that most SHS conditions in humans are close to body temperature, SHS of 39°C was adopted to build the fmSHS model. fmSHS reduced the fertilization rate, impaired on-time development and reduced the implantation rate of the embryos in the IVF–ET process, but it did not affect the development or function of blastocysts . The fmSHS mice produced more Y chromosome-bearing spermatozoa and propagated more male offspring. Conclusions fmSHS not only reduced the fertilization ability of spermatozoa but also influenced their function beyond fertilization, in addition to changing the offspring sex ratio. These results may help to shed new light on the infertility treatment of males with scrotal heat risk and the health concerns of offspring propagated from these fathers.
研究领域:
生殖生物学
·
男科学
·
胚胎学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Plant Science
·
2020
·
IF 4.402
Wei Shuai-Jie,Chai Sen,Zhu Rui-Min,Duan Cun-Ying,Zhang Yan,Li Sha
Ovules are female reproductive organs of angiosperms, containing sporophytic integuments and gametophytic embryo sacs. After fertilization, embryo sacs develop into embryos and endosperm whereas integuments into seed coat. Ovule development is regulated by transcription factors (TF) whose expression is often controlled by microRNAs. Mutations of Arabidopsis DICER-LIKE 1 (DCL1), a microRNA processing protein, caused defective ovule development and reduced female fertility. However, it was not clear whether other microRNA processing proteins participate in this process and how defective ovule development influenced female fertility. We report that mutations of HUA ENHANCER1 (HEN1) and HYPONASTIC LEAVES 1 (HYL1) interfered with integument growth. The sporophytic defect caused abnormal embryo sac development and inability of mutant ovules to attract pollen tubes, leading to reduced female fertility. We show that the role of HEN1 in integument growth is cell-autonomous. Although AUXIN RESPONSE FACTOR 6 (ARF6) and ARF8 were ectopically expressed in mutant ovules, consistent with the reduction of microRNA167 in hen1, introducing arf6;arf8 did not suppress ovule defects of hen1, suggesting the involvement of more microRNAs in this process. Results presented indicate that the microRNA processing machinery is critical for ovule development and seed production through multiple microRNAs and their targets.
研究领域:
植物学
·
遗传学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2025
·
IF 4.5
Chunfang Wang,Yining Wang,Faqiong Zhao,Baizhao Zeng
Organic-inorganic composite photoactive materials can combine the merits of organic and inorganic components and show better performance in comparison with single-component materials. In this work, a novel photoactive material was synthesized through chemical polymerization of 3,4-ethylenedioxythiophene (EDOT) on the surface of hydrothermally prepared Bi 2 WO 6 , followed by modification of self-polymerized dopamine (PDA). The integration of poly(3,4-ethylenedioxythiophene) (PEDOT) and Bi 2 WO 6 effectively facilitated the separation of photogenerated electron-hole pairs, thus demonstrated substantially strong photoelectrochemical signal compared to pure Bi 2 WO 6 . The PDA film, with abundant functional groups, could further sensitize the material and facilitate the immobilization of recognition units. As a proof of application, the composite was combined with carcinoembryonic antibody to construct an immunosensor for carcinoembryonic antigen sensing. Resultantly, although the components and construction procedure were simple, the prepared sensor demonstrated a broad linear detection range of 0.001–20 ng/mL and a relative low detection limit of 0.50 pg/mL. It also showed high selectivity and stability. Its practical feasibility was confirmed by detecting real samples and yielding satisfactory results.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomolecules
·
2021
·Vol. 11
·期 8
·1133
·
IF 4.879
Kevin Adi Kurnia,Fiorency Santoso,Bonifasius Putera Sampurna,Gilbert Audira,Jong-Chin Huang,Kelvin H.-C. Chen,Chung-Der Hsiao
Tail coiling is a reflection response in fish embryos that can be used as a model for neurotoxic analysis. The previous method to analyze fish tail coiling is largely based on third-party software. In this study, we aim to develop a simple and cost-effective method called TCMacro by using ImageJ macro to reduce the operational complexity. The basic principle of the current method is based on the dynamic change of pixel intensity in the region of interest (ROI). When the fish tail is moving, the average intensity is increasing. In time when the fish freeze, the peak of mean intensity is maintaining at a relatively low level. By using the optimized macro settings and excel VBA scripts, all the tail coiling measurement processes can be archived with few operation steps with high precision. Three major endpoints of tail coiling counts, tail coiling duration and tail coiling intervals can be obtained in batch. To validate this established method, we tested the potential neurotoxic activity of Tricaine (methanesulfonate, MS-222) and psychoactive compound of caffeine. Zebrafish embryos after Tricaine exposure displayed significantly less tail coiling activity in a dose-dependent manner, and were comparable to manual counting through the Wilcoxon test and Pearson correlation double validation. Zebrafish embryos after caffeine exposure displayed significantly high tail coiling activity. In conclusion, the TCMacro method presented in this study provides a simple and robust method that is able to measure the relative tail coiling activities in zebrafish embryos in a high-throughput manner.Keywords:ImageJ;chorion;tail coiling;ROI;zebrafish;embryo
研究领域:
发育生物学
·
毒理学
✓ 已验证
DOI
PubMed
高影响因子
Food and Bioprocess Technology
·
2024
·
IF 5.3
Hu Jin,Yang Wencheng,Lan Huanling,Lin Xueyuan,Li Chang
Rice ( Oryza sativa ) bran is a by-product of rice processing, which contains rich nutrients and is a resource that can be fully utilized. However, lipase in rice bran can lead to rancidity of rice bran oil. Therefore, it is important to study the characteristics of rice bran lipase for the stable storage of rice bran. A new lipase from rice bran was isolated by DEAE Sepharose column and gel filtration column, respectively. The determination of lipase’s molecular weight and purity was conducted utilizing the SDS-PAGE technique, and the enzyme was identified through LC-MS/MS. Concurrently, an investigation was conducted on the characteristics of the lipase. The findings indicated that the lipase had a molecular weight of 36.352 kDa. Optimal catalytic activity for rice bran lipase (RBL) was observed at a temperature of 40 ℃ and pH value of 7.0, while it exhibited remarkable stability below 20 ℃ and within the pH range from 6.0 to 8.0. The stability of the RBL against various metal ions and high NaCl concentrations was observed to be relatively modest. Notably, the catalytic activity was significantly influenced by certain organic solvents (such as acetone, THF, and trichloromethane) as well as several inhibitors ( P < 0.05). Furthermore, RBL demonstrated a preference for hydrolyzing long-chain p-NP esters (C12-C16).
研究领域:
生物化学
·
食品科学
✓ 已验证
DOI
高影响因子
Reproductive Biology
·
2024
·
IF 2.5
Romualdo Sciorio,Pier Francesco Greco,Mohammed Adel,Lucia Maresca,Ermanno Greco,Steven Fleming
In the last decades, to enhance success rates in assisted reproductive technology (ART) cycles, scientists have continually tried to optimize embryo culture and selection to increase clinical outcomes. In this scenario, the application of laser technology has increased considerably worldwide and is currently applied across ART in several ways: for assisted hatching (AH) or thinning of the zona pellucida (ZP), embryo biopsy, to immobilize and select the sperm during intracytoplasmic sperm injection, as well as to induce artificial blastocyst shrinkage before cryopreservation. Laser-AH has been suggested as a procedure to improve embryo implantation: the concept is that drilling holes through or thinning of the ZP could improve the hatching process and implantation. The artificial disruption of the ZP can be performed by different approaches: mechanically, chemically and with the laser, which is one of the most favourable and easy methods to remove part of the ZP and to augment the possibilities of implantation in patients defined as having a poor prognosis of success, or when the ZP is too thick. However, in the current literature, there is not sufficient evidence about the potential risk or impairment that laser utilization might induce on embryo development; therefore, the main aim of the current review is to provide an overview of the existing knowledge on the ZP and the mechanisms of manipulating it to improve the effectiveness of ART. Also, it emphasizes the positive aspect of laser application as a powerful tool that might increase the chance of pregnancy for infertile couples undergoing ART cycles.
研究领域:
生殖生物学
·
辅助生殖技术
·
胚胎学
✓ 已验证
DOI
PubMed
高影响因子
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