ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Xingkang Diao,GuoHua Qi,Xinli Li,Yu Tian,Jing Li,Yongdong Jin
Cell cycle analysis is crucial for disease diagnosis and treatment, especially for investigating cell heterogeneity and regulating cell behaviors. Exosomes are highly appealing as noninvasive biomarkers for monitoring real-time changes in the cell cycle due to their abundant molecular information inherited from their metrocyte cells and reflecting the state of these cells to some extent. However, to our knowledge, the relationship between exosomes and the cell cycle has not been reported. Herein, we successfully monitored the variation of exosomal surface-enhanced Raman spectroscopy (SERS) spectra to discriminate different cell cycle stages (G0/G1, S, and G2/M phases) based on label-free surface-enhanced Raman spectroscopy (SERS) combined with the machine learning method of linear discriminant analysis (LDA). An average accuracy of 85% based on the trained SERS spectra of exosomes from different cell cycle stages confirmed the high reliability of the support vector machine (SVM) algorithm for analyzing dynamic changes in the cell cycle at different time points. Importantly, the related molecular mechanisms among mitotic processes (prometaphase, metaphase, and anaphase/telophase) and unique biomolecular events between cancerous (HeLa) and normal (H8) cells were also revealed by the present label-free SERS detection platform. Based on SERS analysis, the content of phenylalanine (Phe) within HeLa cells increased, and some structures of proteins containing Phe and tryptophan (Trp) residues may be transformed during the mitotic process. Notably, the α-helix and β-sheet of proteins coexisted in HeLa cells; meanwhile, the α-helix of the proteins was more dominant in H8 cells than in HeLa cells. The strategy is effective for discriminating cell cycle stages and elucidating the associated molecular events during the cell mitotic process and will provide potential application value for guiding the cell cycle treatment strategies of cancer in the future.
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高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2025
·
IF 6.3
Jianbo Zhang,Yidong Zhong,Yue Feng,Meijun Chen
Using thymine as a structure-directing agent, this study successfully prepared trimetallic platinum-palladium-copper nano-dendrites (PdPtCu NDs) with a highly branched architecture for plasmonic catalytic applications. The material exhibits a uniform and well-dispersed nano-spike structure, significantly increasing its specific surface area and exposing more active sites. At the atomic scale, the close integration of platinum, palladium, and copper, along with the modulation of the electronic structure, synergistically optimizes the material's physicochemical properties. Under near-infrared (NIR) irradiation, the PdPtCu NDs effectively convert incident light energy into heat through surface plasmon resonance and metal-metal synergistic effects, rapidly increasing the system's temperature and significantly enhancing the efficiency of H 2 O 2 decomposition into hydroxyl radical (•OH). This characteristic suggests a strong synergistic effect with Fenton-like catalytic performance, thereby accelerating the oxidative degradation of dye molecules. This coupling effect overcomes several limitations of traditional Fenton reactions, including low efficiency, the need for acidic conditions, and the generation of iron precipitates.
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FISH PHYSIOLOGY AND BIOCHEMISTRY
·
2026
·Vol. 52
·期 1
·11
·
IF 2.5
Yu Jinzhi,Wang Naixuan,Liu Lijun,Zhou Hong,Zhang Anying,Wang Xinyan
S100A10 protein, a member of S100 protein family, has been extensively studied in mammalian models. In fish species, evolution of S100 proteins including S100A10 has been clarified, revealing that two paralogous genes, S100A10a and S100A10b , arose in teleosts through the fish-specific whole-genome duplication event. However, the functional roles of fish S100A10 genes remain largely uncharacterized. In the present study, we identified two S100A10 homologs in grass carp, designated gcS100A10a and gcS100A10b, and demonstrated that their mRNA is highly expressed in grass carp skin and strongly upregulated after skin trauma by real-time quantitative PCR. Using the fish epithelial cell line, Epithelioma papulosum cyprini (EPC) cells, as a model, we showed that overexpression of gcS100A10a or gcS100A10b enhances cell viability, increases expression of proliferating cell nuclear antigen (PCNA), and promotes DNA synthesis, thereby demonstrating their capacity to stimulate cell proliferation. Flow cytometric analysis further uncovered that both proteins accelerate EPC cell cycle progression by promoting transition into the S and G2/M phases. Additionally, gcS100A10a and gcS100A10b significantly elevate EPC cell migration and this elevation is attenuated by a broad-spectrum matrix metalloproteinase (MMP) inhibitor, GM6001, suggesting involvement of MMP activity in this process. This suggestion is strongly supported by findings that overexpression of gcS100A10a or gcS100A10b increases extracellular MMP activity and decreases intracellular MMP accumulation although MMP-2 / MMP-9 mRNA levels remain unchanged. Collectively, these results demonstrate the positive regulation of gcS100A10a and gcS100A10b on fish epithelial cell proliferation and migration, together with their high and injury-inducible expression in grass carp skin, indicating their possible involvement in fish skin wound healing.
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高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2023
·
IF 4.1
Li Gao,Yuan Wang,Yaqiong Qin,Yaming Sun,Lijun He,Shusheng Zhang,Wenjie Zhao
Carbohydrate is a renewable, sustainable, hydrophilic, and biodegradable natural product, which is widely used in the field of adsorption. In this study, a glucose-based covalent organic polymer (COP) coated silica was fabricated by facile solvent knitting reaction between tetrabenzylglucose and silica-phenyl with anhydrous aluminum trichloride as catalyst, forming a core-shell stationary phase (donated as SiO 2 @COP Bn-glu ) for high performance liquid chromatography. The prepared SiO 2 @COP Bn-glu was characterized by scanning electron microscopy, transmission electron microscopy, Fourier-transform infrared spectrometry, solid-state 13 C nuclear magnetic resonance spectrometry, X-ray photoelectron spectroscopy, and N 2 adsorption-desorption experiments. Owing to the coexistence of benzene units and alkyl, hydroxyl and ether groups in the skeleton of COP Bn-glu shell, the developed chromatographic packing exhibited reversed-phase/hydrophilic interaction mixed-mode with multiple retention mechanisms, such as hydrophobic, π-π, hydrogen bonding, and electron donor-acceptor interactions. The results revealed that the SiO 2 @COP Bn-glu column demonstrated excellent selectivity and retention behavior for both hydrophilic and hydrophobic compounds with good repeatability and stability. Meanwhile, the chromatographic performance of the prepared SiO 2 @COP Bn-glu column was compared with a C 18 column to assess the role of the coating COP Bn-glu shell. Therefore, the development of the SiO 2 @COP Bn-glu stationary phase expands the potential application of COPs in separation field.
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高影响因子
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
·
2023
·Vol. 39
·期 10
·1-9
·
IF 4.1
Xu Lian,Li Hui-Min,Lin Juan
2’-deoxyguanosine is a key medicinal intermediate that could be used to synthesize anti-cancer drug and biomarker in type 2 diabetes. In this study, an enzymatic cascade using thymidine phosphorylase from Escherichia coli ( Ec TP) and purine nucleoside phosphorylase from Brevibacterium acetylicum ( Ba PNP) in a one-pot whole cell catalysis was proposed for the efficient synthesis of 2’-deoxyguanosine. Ba PNP was semi-rationally designed to improve its activity, yielding the best triple variant Ba PNP-Mu3 (E57A/T189S/L243I), with a 5.6-fold higher production of 2’-deoxyguanosine than that of wild-type Ba PNP ( Ba PNP-Mu0). Molecular dynamics simulation revealed that the engineering of Ba PNP-Mu3 resulted in a larger and more flexible substrate entrance channel, which might contribute to its catalytic efficiency. Furthermore, by coordinating the expression of Ba PNP-Mu3 and Ec TP, a robust whole cell catalyst W05 was created, capable of producing 14.8 mM 2’-deoxyguanosine (74.0% conversion rate) with a high time-space yield (1.32 g/L/h) and therefore being very competitive for industrial applications.
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TALANTA
·
2023
·
IF 6.1
Fei Wang,Fan Yang,Jiawei Liu,Quan Bai
This paper is a continuation of research into the retention behavior and mechanism of solutes in hydrophilic interaction chromatography (HILIC) using stoichiometric displacement theory (SDT). A HILIC/reversed-phase liquid chromatography (RPLC) dual-retention mechanism was studied in detail using a β -CD HILIC column. The retention behaviors of three groups of solutes with varying polarities were studied over the entire range of water concentrations in the mobile phase on the β -CD column, resulting in the formation of “U-shape” curves when lg k ’ was plotted against lg[H 2 O]. Additionally, the effect of hydrophobic distribution coefficient lg P O/W on the retention behaviors of solutes in HILIC and RPLC modes was also examined. A four-parameter equation derived from the SDT-R was found to accurately describe the “U-shaped” curves of solutes with RPLC/HILIC dual-retention mechanisms on β -CD column. The theoretical lg k ’ values of solutes calculated using the equation were found to be in agreement with their experimental values, with correlation coefficients greater than 0.99. This indicates that the four-parameter equation derived from SDT-R can effectively describe the retention behaviors of solutes over the entire range of water concentrations in the mobile phase in HILIC. As such, SDT can be used as a theoretical guide for the development of HILIC, including the exploration of new dual-function stationary phases to enhance separation efficiency.
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MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Zhihua Song,Jinqiu Liu,Deyang Zhang,Yumeng Wang,Wen Lin,Yi Bi,Hongbo Wang,Maryam Arabi,Jinhua Li,Jiping Ma,Yan Wang,Lingxin Chen
Up to now, the chromatographic separation and analysis of highly polar compounds are still challenging because of low retention ability and selectivity of conventional stationary phases (SPs). Developing new SPs to achieve high-resolution and high-efficiency separation of polar compounds is of great importance for health and medicine, food safety, environmental protection, etc. . Herein, an influential multi-step modification strategy (modification with 1H,1H,2H,2H-perfluorooctyltriethoxysilane on the silica twice and end-capped with trimethylchlorosilane once) is introduced to prepare C8F13-SiO 2 SP. The physical properties of C8F13-SiO 2 SP, including morphologies, pore structures and carbon content were fully characterized by various instrumental techniques. The packed HPLC column with C8F13-SiO 2 SP exhibited high column efficiency, good peak shape (tailing factors for most analytes are between 0.9 and 1.3), and resolution for simultaneous separation of ginsenosides, sulfonamides, nucleosides , and benzoylurea insecticides (BUs); meanwhile, the retention mechanism of typical reversed-phase liquid chromatography (RPLC) mode was observed. A column efficiency of 30,220 plates/m for separation of ginsenoside Re was obtained. Furthermore, the resulting columns benefit from the advantages of supreme reproducibility, repeatability and stability. Importantly, the chromatographic properties (e.g. column efficiencies, chromatographic peak shapes, retention ability and resolution) of the C8F13-SiO 2 SP for the polar analytes were superior to those of the commercial SPs (including commercial amino-modified silica SP, diol-modified silica SP and commercial PFP-SiO 2 SP). Thus, the C8F13-SiO 2 SP is able to satisfy the strict requirements of highly polar compounds separation.
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JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Kaixing Luo,Yu Luo,Yanjuan Liu,Yuefei Zhang,Wei Chen,Zhengwu Bai,Sheng Tang
s In this work, a new strategy of simultaneously grafting two different functional ligands onto the silica surface was adopted to prepare a carbonyldiimidazolium/dodecyl dual-functionalized silica stationary phase (Sil-CDI-DD) by recycling the discarded commercial C18 columns as the raw materials. A full characterization of the waste silica-C18, the calcined silica, the acidified silica and the new packing materials was done to explore the feasibility of recycling the waste chromatographic columns. Hydrophobic and hydrophilic selectivity of the obtained Sil-CDI-DD was specifically investigated, and retention mechanisms in reversed-phase and hydrophilic interaction chromatography modes were discussed. The experimental results showed that the perfect combination of carbonyldiimidazolium and long-chain alkyl groups commendably enhanced the separation selectivity and efficiency of the versatile Sil-CDI-DD, which could provide multiple interaction sites for diverse target analytes, thereby contributing to the favorable resolution of hydrophilic nucleosides/nucleobases and weak polar environmental endocrine disruptors , including alkylphenols, bisphenols , steroid hormones and phthalates .
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高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Yan Wu,Kaixing Luo,Yanjuan Liu,Wei Chen,Zhengwu Bai,Sheng Tang
Developing a versatile stationary phase to achieve the purpose of efficiently separating various target analytes is of great significance. In this work, ureido/dodecyl dual-functionalized silica (Sil-Ur-DD) was synthesized and employed as a new mixed-mode stationary phase for high-resolution chromatographic analysis. It was observed that multiple interactions, including hydrophobic, hydrophilic, hydrogen bonding, electrostatic and π-π interactions, contributed to the retention. Nucleosides and nucleobases could be effectively determined in hydrophilic interaction chromatography mode, and the separation performance of the Sil-Ur-DD was found to be even superior to that of the commercial XAmide column. Environmental endocrine disruptors, including alkylphenols, bisphenols, steroid hormones and phthalates, were more favorably separated in reversed-phase chromatography mode with highly aqueous mobile phases compared with the commercial C18-silica column. The experimental results indicated that the combined effect of ureido and long-chain alkyl groups obviously enhanced separation efficiency and selectivity. More significantly, the proposed method concerning the preparation of an efficient separation material with powerful separation ability is economical and environmentally friendly, which provides a proven and effective way for the recycling and utilization of the discarded chromatographic packing materials, thereby showing a good application prospect.
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MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Ye Shu,Tong Chen,Tao Liu,Hechen Xu,Jinjin He,Zhihui Cai,Wanning Zhu,Zihan Zhao,Jun Jiang
In this paper, the surface modification of spherical silica was sequentially grafted with functional groups including imidazole, amide and sulfonic acid. The novel surface polymerization type stationary phases (SPs) for hydrophilic interaction liquid chromatography (HILIC) were obtained in three procedures. Elemental analysis, Fourier transform infrared spectroscopy, transmission scanning electron microscopy, Brunauer Emmett Teller and Barrett Joyner Halenda were carried out for the characterization of the SPs. The bonding rate of the functionalized group (1-acrylamide propyl sulfonic) was calculated to be 2.02 μmol/m 2 . Positive outcomes were attained for the surface grafting of the SPs. The effect of various chromatographic conditions on the retention time of various analytes was investigated on high performance liquid chromatography. The dual retention mechanism of hydrophilic interaction and ion exchange mechanism of the prepared SPs was confirmed. The polymeric HILIC SPs had more abundant functionalized groups on the surface, which provided superior hydrophilic properties, ion exchange and thus higher selectivity. Compared with the C 18 column, the resolution of the HILIC column was better. The HILIC SPs were used for the separation of sulfonamides and flavonoid natural components, and had achieved better baseline separations. In addition, the HILIC mode was used for the separation of the extract of the Fructus Ligustri Lucidi , in which the quality markers had satisfactory peak shape and baseline separation. In conclusion, the prepared surface polymeric SPs were competent for the analysis of polar components and applicable to complex samples.
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MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Tong Chen,Liang Xu,Guangsan Song,Yuan Li,Hong Xu,Hongbin Zhou,Zhen Xiao,Ping Li
Au nanoparticles (Au NPs)-decorated SO 3 H-cofunctionalized porous silica particles was prepared as a novel mixed-mode stationary phase and used in Per aqueous liquid chromatography (PALC) mode. The obtained stationary phase was characterized by elemental analysis, FT-IR spectra, and transmission electron microscopy. To evaluate the retention behavior, the effect of a variety of chromatographic factors on the retention of different types of solutes was investigated. The obtained column exhibited a hydrophobic interaction/cation-exchange mixed-mode retention mechanism, and a typical PALC characteristic was observed in water-rich mobile phases. When the particle size of Au NPs was 10–20 nm, the analytes had a sharp peak shape and the column pressure was also moderate. Compared with the hydrophilic interaction liquid chromatography (HILIC) and C 18 column, some hydrophilic compounds such as six monosaccharides were baseline separated with the enhanced resolution of the obtained column under the PALC mode. Moreover, the efficiency of the Au-S/SO 3 H-SiO 2 -2 column was significantly higher than that of the HILIC column. PALC mode was a green annlysis method and suitable for the replacement of hydrophilic interaction liquid chromatography.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Jia Ding,Fei Liu,Chunling Qi,Yunlei Zhou,Huanshun Yin,Shiyun Ai
A novel photoelectrochemical (PEC) biosensor was constructed for DNA hydroxymethylation detection based on Bi 4 NbO 8 Cl/PTC-NH 2 heterojunction and Fe-MIL-88NH 2 , where Bi 4 NbO 8 Cl/PTC-NH 2 was employed as photoactive material and Fe-MIL-88NH 2 was adopted as artificial hydrogen peroxide mimic enzyme. Based on the structure of hydroxymethylated cytosine in DNA sequence, 5-hydroxymethyl-2’-deoxycytidine (5hmdC) was employed as detected target molecule for the first time using PEC technique. After Bi 4 NbO 8 Cl/PTC-NH 2 heterojunction was modified on the substrate electrode, thioglycolic acid (TGA) was captured by forming amide bond, where the sulfhydryl group (-SH) of TGA was employed as the specific recognition of 5hmdC. Under M. HhaI catalysis, the −CH 2 OH of 5hmdC reacted selectively with the –SH. Finally, Fe-MIL-88NH 2 was modified on the electrode based on the crosslinker of 4-formylphenylboronic acid (FPBA), where FPBA possessed two active groups of formyl and boronic acid group, reacting with glycosyl of 5hmdC and amino of Fe-MIL-88NH 2 . Under the catalysis effect of Fe-MIL-88NH 2 , the insoluble substance was produced on the surface of electrodes, resulting in distinct lessening of PEC response. Under optimal conditions, the photocurrent shows a linear relationship with the logarithm value of 5hmdC concentration from 0.01-100 nM with a low detection limit of 6.48 pM. More importantly, this method can be applied to investigate the influence of exogenous phytohormone on 5hmdC content in the genomic DNA of rice seedling roots and leaves.
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