PHYTOMEDICINE
·
2026
·
IF 11.3
Xiaojue Xiang,Xu Lin,Qifang Wu,Xiaoming Zhang,Xueliang Fu,Linya Wu,Haibin Tong,Zhenyu Dai
Dendrobium officinale extract DOE ameliorates metabolic disorders and diabetic cardiomyopathy. • DOE reduced myocardial injury, fibrosis, and oxidative stress via activating NRF2 pathway. • Dendrobine was the principal cardioprotective alkaloid in DOE against diabetic cardiomyopathy . • Dendrobine directly targets KEAP1 Kelch domain to disrupt the KEAP1-NRF2 interaction . Background Dendrobium officinale Kimura et Migo is a prestigious traditional Chinese medicine historically used for treating "Xiao-Ke" (diabetes and its complications). However, the specific material basis and direct molecular targets responsible for its efficacy against diabetic cardiomyopathy (DCM) remain largely obscure. This study aimed to systematically investigate whether dendrobine, the characteristic active alkaloid of DO, serves as the principal cardioprotective component by directly targeting the KEAP1-NRF2 signaling axis. Methods The therapeutic effects of D. officinale extract (DOE) and dendrobine were evaluated in vivo using db/db mice and in vitro using high-glucose (HG)-challenged H9c2 cardiomyocytes. Cardiac pathological remodeling, lipid peroxidation, and systemic metabolic profiles were comprehensively assessed. The direct interaction between dendrobine and KEAP1 was definitively characterized using Co-Immunoprecipitation (Co-IP), Cellular Thermal Shift Assay (CETSA), Drug Affinity Responsive Target Stability (DARTS), MicroScale Thermophoresis (MST), and 100-ns Molecular Dynamics (MD) simulations. Reverse pharmacological validation was performed using NRF2-specific siRNA and the inhibitor ML385. Results In vivo , DOE significantly ameliorated systemic insulin resistance, hyperlipidemia, and myocardial pathological remodeling in db/db mice while robustly suppressing myocardial oxidative stress (MDA, 4-HNE) by upregulating NRF2-driven antioxidant enzymes (HO-1, NQO1, GPX4). In vitro , dendrobine dose-dependently replicated these cardioprotective and antioxidative efficacies. Mechanistically, dendrobine promoted NRF2 nuclear translocation and initiated the downstream antioxidant cascade. Crucially, MST and CETSA revealed that dendrobine binds directly to KEAP1 with high affinity ( K d = 1.18 μM), functioning as a competitive protein-protein interaction (PPI) inhibitor to disrupt the KEAP1-NRF2 complex. MD simulations and mutagenesis validation pinpointed the Kelch domain of KEAP1 (specifically residue Arg415) as the binding pocket. Finally, silencing or inhibiting NRF2 abolished the anti-hypertrophic and cytoprotective effects of dendrobine, and the in vivo administration of dendrobine successfully recapitulated the metabolic regulation and KEAP1/NRF2-mediated cardioprotection of DOE in db/db mice. Conclusion Dendrobine, the principal alkaloid of D. officinale , effectively ameliorates diabetic cardiomyopathy by functioning as a novel, direct competitive inhibitor of the KEAP1-NRF2 interaction. This targeted disruption liberates NRF2 to restore myocardial redox homeostasis, highlighting dendrobine as a promising lead compound for DCM therapy. Download: Download high-res image (395KB) Download: Download full-size image
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Food Innovation and Advances
·
2026
·Vol. 5
·期 3
·419-431
·
IF 5.4
Xueyan Zhang,Zi Li,Lixiang Zhu,Junjie Yi,Linyan Zhou
This study investigated the combined effects of high-pressure processing and Phyllanthus emblica (PE) juice supplementation on enzymatic browning and nutritional quality of apple juice. All PE-treated groups delayed enzymatic browning. After 35 d, the 10% PE group showed a 0.17-fold higher L* value, and 1.27- and 0.29-fold lower a* and b* values, respectively. The HPP-PE treatment suppressed polyphenol oxidase (PPO) and peroxidase (POD), with 10% PE reducing initial PPO and POD activities by 28% and 24%, respectively, and remaining 23.81% and 26.25% lower by day 35. PE supplementation enhanced biofunctional activities. In the 10% PE group, total phenol and flavonoid contents increased by 13.5- and 3.78-fold on day 0, and by 28.75- and 6.4-fold on day 35, respectively. Correspondingly, ABTS and DPPH radical scavenging activities increased by 18.23- and 15.35-fold on day 0, and by 20.56- and 33.81-fold on day 35. Twenty phenols were identified in PE juice, predominantly ellagic acid, trigalloyl-hexoside, ellagic acid 4-O-β-D-glucoside, and geraniin. Molecular docking indicated that hydrogen bonding and ionic interactions were the key forces inhibiting PPO activity. By integrating high-resolution metabolite profiling with molecular docking, this study elucidated the structure–function relationships underlying PE-derived browning inhibition, offering a mechanistically grounded, clean-label strategy for NFC juice preservation.
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JOURNAL OF FOOD SCIENCE
·
2026
·Vol. 91
·期 7
·e71273
·
IF 4.1
Jie Liu,Yuxiao Wang,Xin Sun,Qiyong Jiang,Hongxun Tao,Na Li,Wai San Cheang,Rentang Zhang
Previous studies have established that the blackening process significantly enhances both the flavor profile and the content of bioactive compounds in jujubes. Fruit vinegars, which are rich in antioxidants and organic acids, are recognized for their role in supporting digestion and enhancing metabolism. To fully leverage these augmented benefits, the present study developed and comparatively analyzed red jujube vinegar (RJV) and blackened jujube vinegar (BJV). Our results show that BJV attained a significantly higher total acidity (3.73 g/100 mL) compared to RJV (3.33 g/100 mL) and demonstrated a continuous accumulation of polyphenols and flavonoids. A model is proposed in which polyphenols in BJV undergo structural transformation, synergizing with acids generated by Acetobacter to form a distinctive “phenol-acid” antioxidant system. Volatile compound analysis revealed that the smoother, sweet-and-sour flavor profile of BJV is attributable not only to the synergy between organic acids and esters but also to a unique ensemble of characteristic volatiles identified exclusively in BJV. This ensemble includes a series of acids (e.g., 4-methylpentanoic acid), alcohols (e.g., isoamyl alcohol), and esters, with ethyl acetate notably contributing intense banana and pear-like fruity notes. These findings provide a scientific basis for developing high-value, health-promoting vinegar products from blackened jujubes.
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PLANT PHYSIOLOGY AND BIOCHEMISTRY
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2025
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IF 5.7
Jiafei Qian,Wenwen You,Yicheng Wang,Yunlin Cao,Xiaoyong Zhao,Hongxia Xu,Hang Ge,Xiaoying Li,Di Wu,Junwei Chen,Xian Li
Loquat ( Eriobotrya japonica ) is rich in flavonols with health-promoting functions. However, regulatory mechanisms of flavonol biosynthesis in loquat are unclear. This study functionally characterized an R2R3-MYB, EjMYBF1 from loquat. Expression level of EjMYBF1 is positively correlated with flavonol content at different stages of fruit development and in ten accessions with differential flavonol accumulation. Dual-luciferase assays and EMSA indicated that EjMYBF1 transactivated the promoters of structural genes involved in flavonol biosynthesis by binding to promoter regions containing MYBCORE elements. Transient overexpression and virus-induced gene silencing assays of EjMYBF1 in loquat fruit peels and leaves, as well as heterologous constitutive expression of EjMYBF1 in Nicotiana tabacum confirmed that EjMYBF1 is a positive regulator of flavonol biosynthesis in loquat. These results demonstrate that EjMYBF1 is a functional flavonol-specific activator in loquat and explain one reason for its high flavonol content from the perspective of transcriptional regulation. This work provides a potential target for improving loquat nutrition and for applications in secondary metabolic engineering.
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PHYSIOLOGIA PLANTARUM
·
2026
·Vol. 178
·期 2
·e70832
·
IF 4
Lihong Jiang,Huanwen Chen,Dan Xie,Xiaowei Chen,Jianjiao Xu,Niya Jia,Zikai Pan,Yajing Wang,Yan Dai,Defu Chi,Jia Yu
Abiotic stresses such as high salinity or alkalinity usually alter plant secondary metabolism, thereby affecting their defense against herbivorous insects. This study investigated the effects of changes in terpenoids and phenolics of black pine ( Pinus thunbergii ) induced by salt, alkali, and mixed saline–alkali stresses on the host selection, host fitness, and laboratory bioassays of red-haired bark beetle ( Hylurgus ligniperda ). Field experiments showed that saline–alkali stress resulted in a decreased proportion of (1R)-(+)-α-pinene, an increased proportion of β-pinene and sabinene, and reduced contents of tannins, total flavonoids, and total phenols in the phloem of P. thunbergii , and promoted the damage of H. ligniperda to P. thunbergii . Laboratory bioassays showed that changes in terpenoids had a significant impact on the electroantennogram (EAG) and behavioral responses of H. ligniperda , while changes in phenolics had little effect on its growth and development. Based on Partial Least Squares Path Model (PLS-PM) comparison and laboratory bioassays results, terpenoids were identified as the main factor promoting damage of H. ligniperda to P. thunbergii . Specifically, saline–alkali stress enhances the host-locating ability of H. ligniperda , leading to an increase in its population density and thus promoting damage to P. thunbergii . This study provides insights into the complex interactions between plant physiology and insect behavior under abiotic stress, delivering more precise strategies for pest management in salinized–alkaline forest environments.
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Antioxidants
·
2023
·Vol. 12
·期 3
·552
·
IF 7
Abid Naeem,Chengqun Yu,Zhenzhong Zang,Weifeng Zhu,Xuezhen Deng,Yongmei Guan
Oxidants play a significant role in causing oxidative stress in the body, which contributes to the development of diseases. Rutin—a powerful antioxidant—may be useful in the prevention and treatment of various diseases by scavenging oxidants and reducing oxidative stress. However, low solubility and oral bioavailability have restricted its use. Due to the hydrophobic nature of rutin, it cannot be easily loaded inside hydrogels. Therefore, first rutin inclusion complexes (RIC) with hydroxypropyl-β-cyclodextrin (HP-βCD) were prepared to improve its solubility, followed by incorporation into xanthan gum-based (hydroxypropyl methylcellulose-grafted-2-acrylamido -2-methyl-1-propane sulfonic acid) hydrogels for controlled drug release in order to improve the bioavailability. Rutin inclusion complexes and hydrogels were validated by FTIR, XRD, SEM, TGA, and DSC. The highest swelling ratio and drug release occurred at pH 1.2 (28% swelling ratio and 70% drug release) versus pH 7.4 (22% swelling ratio, 65% drug release) after 48 h. Hydrogels showed high porosity (94%) and biodegradation (9% in 1 week in phosphate buffer saline). Moreover, in vitro antioxidative and antibacterial studies (Staphylococcus aureus,Pseudomonas aeruginosa, andEscherichia coli) confirmed the antioxidative and antibacterial potential of the developed hydrogels.Keywords:hydrogels;flavonoids;anti-oxidants;inclusion complexes;oral formulations
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Nutrients
·
2023
·Vol. 15
·期 5
·1078
·
IF 5.9
Shengxiang Yi,Gaowei Zhang,Mingyan Liu,Wenjie Yu,Guohua Cheng,Liping Luo,Fangjian Ning
Citrus honey (CH) is rich in nutrients that have a wide variety of biological functions, such as antibacterial, anti–inflammatory, and antioxidant activities, and which demonstrate therapeutic properties, such as anti–cancer and wound–healing abilities. However, the effects of CH on alcohol–related liver disease (ALD) and the intestinal microbiota remain unknown. This study aimed to determine the alleviating effects of CH on ALD and its regulatory effects on the gut microbiota in mice. In total, 26 metabolites were identified and quantified in CH, and the results suggested that the primary metabolites were abscisic acid, 3,4–dimethoxycinnamic acid, rutin, and two markers of CH, hesperetin and hesperidin. CH lowered the levels of aspartate aminotransferase, glutamate aminotransferase, and alcohol–induced hepatic edema. CH could promote the proliferation of Bacteroidetes while reducing the abundance of Firmicutes. Additionally, CH also showed some inhibitory effects on the growth of Campylobacterota andTuricibacter. CH enhanced the secretion of short–chain fatty acids (SCFAs), such as acetic acid, propionic acid, butyric acid, and valeric acid. Given its alleviating functions in liver tissue damage and its regulatory effects on the gut microbiota and SCFAs, CH could be a promising candidate for the therapeutic treatment of ALD.Keywords:citrus honey;gut microbiota;alcohol–related liver disease;short–chain fatty acids
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JOURNAL OF SEPARATION SCIENCE
·
2023
·Vol. 46
·期 11
·2300037
·
IF 3.1
Yue Xiong,Zhuhui Chen,Peng-Bo Duan,Ranran Ma,Gisèle Ineza Urujeni,E-Hu Liu,Deli Xiao
In the classical natural product extraction and separation process, it is tedious and requires large amounts of reagents and time. In this study, an efficient coaxial liquid centrifugal oil-water-oil triple-liquid-phase system with a simple structure and convenient operation was successfully constructed and used to extract flavonoids from Platycladi Cacumen. The results showed that the coaxial liquid centrifugal platform constructed in this study had good stability and 6 ml was the minimum volume of the middle phase for 1000 rpm to stabilize the system. Besides, it was easy to repeat the operation: the relative standard deviations of the extraction yields of flavonoids and sugar in six parallel operations were all less than 10%. Moreover, it was only one-tenth of the time required for this method as traditional liquid-liquid extraction while reducing the use of volatile organic reagents. Finally, the new method was more selective than the traditional method for the extraction of flavonoids. Therefore, this study provides a possibility for the coaxial liquid centrifugal platform to be used in multi-liquid phase systems to achieve the simultaneous extraction of different parts of natural products by different liquid phases. It is expected to provide a reliable reference for further expansion of small-scale experimental operations to industrial production.
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Antioxidants
·
2023
·Vol. 12
·期 3
·664
·
IF 7
Mei Han,Fengxian Yang,Kun Zhang,Jiyan Ni,Xia Zhao,Xuelin Chen,Zhennan Zhang,Hanlei Wang,Jing Lu,Yumei Zhang
Tectona grandisis a traditional Dai medicine plant belonging to the Lamiaceae family, which can be used to treat malaria, inflammation, diabetes, liver disease, bronchitis, tumors, cholelithiasis, jaundice, skin disease and as an anti-helminthic. To find more novel therapeutic agents contained in this medicinal plant, the antioxidant, anti-inflammatory and anti-diabetic activities ofT. grandismethanolic extract, fractions and compounds were evaluated. In this study, 26 compounds were isolated from the leaves and branches ofT. grandis. Their structures were identified based on extensive spectral experiments, including NMR, ESI-MS and comparison with published spectral data. Among them, compounds1–2,4–6,9–14and16–22were reported for the first time for this plant. The antioxidant activity screening results showed that compounds5,15and23had potent antioxidant capacities, with SC50values from 0.32 to 9.92 µmol/L, 0.92 to 1.10 mmol Trolox/L and 1.02 to 1.22 mmol Trolox/L for DPPH, ABTS and FRAP, respectively. In addition, their anti-inflammatory effects were investigated by releasing TNF-α, IL-1βand IL-6 through the use of mouse monocytic macrophages (RAW 264.7). Compounds1,13,18and23had the effects of reducing the expression of inflammatory factors. Compounds13and18were reported for the first time for their anti-inflammatory activities. Furthermore, the methanolic extract (ME), petroleum ether extract (PEE) and EtOAc extract (EAE) ofT. grandisshowed significant glucose uptake activities; compounds21and23significantly promoted glucose uptake of 3T3-L1 adipocytes at 40 µM. Meanwhile, compounds4,5and7showed significant inhibitory activities against α-glucosidase, with IC50values of 14.16 ± 0.34 µmol/L, 19.29 ± 0.26 µmol/L and 3.04 ± 0.08 µmol/L, respectively. Compounds4and5were reported for the first time for theirα-glucosidase inhibitory activities. Our investigation explored the possible therapeutic material basis ofT. grandisto prevent oxidative stress and related diseases, especially inflammation and diabetes.Keywords:Tectona grandis;chemical composition;antioxidant activity;anti-inflammatory activity;α-glucosidase;glucose uptake
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Food Bioscience
·
2023
·
IF 5.2
Qianwei Ma,Yang Yu,Zhongkai Zhou,Lili Wang,Ruge Cao
To take better advantage of the soluble dietary fibers (SDF) of buckwheat bran, different treatments including extrusion, steam explosion, microwave, roasting , superheated steam, ultrasound and enzymolysis were applied to buckwheat bran, and the monosaccharide compositions, structural, physicochemical and biological properties of SDF were estimated. Except enzymolysis treatment, all the other treatments have significantly increased ( p < 0.05) the content of SDF in buckwheat bran. Compared to natural SDF, the SDF in treated buckwheat bran showed porous and wrinkled surface, lower crystallinity, increased thermal stability and better rheological and hydration properties. These treatments have significantly improved the glucose adsorption capacity, nitrite ion adsorption capacity, antioxidant and antibacterial properties of SDF of buckwheat bran, among which extrusion showed the best effect. The microstructure, molecular size, monosaccharide and bound phenolics composition of SDF in buckwheat bran were effectively modified by appropriate treatments, with preferable physicochemical and biological properties.
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NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 16
·7566-7574
·
IF 3.3
Shengbiao Zheng,Tianna Liu,Nini Zhang,Liang Li,Yanli Zhu,Erhui Zhang,Jing Tang,Jiahao Guo
In this report, CoNi-bimetal metal–organic framework (CoNi-MOF) nanosheets with a three-dimensional (3D) flower-like hierarchical structure have been fabricated to modify a glassy carbon electrode and improve its efficiency for determination of quercetin. Scanning electron microscopy, X-ray photoelectron spectroscopy, N2 adsorption–desorption isotherms and impedance spectroscopy were used for the characterization of the CoNi-MOF. Under optimal electrochemical conditions, the anodic peak current measured around 0.44 V (vs. SCE) increased linearly in two linear ranges: 0.05 to 1.0 μM and 1.0–20 μM with a sensitivity of 13.21 μA μM−1 cm2 and 0.934 μA μM−1 cm2, respectively. The excellent electrochemical performance might be attributed to the hierarchical structure, large specific surface area and enhanced electrical conductivity. Finally, the analytical utility of the CoNi-MOF sensor was evaluated by detecting quercetin in human plasma samples.
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Surfaces and Interfaces
·
2023
·
IF 6.2
Yulin Xu,Zhiqiang Xu,Congcong Chen,Weihao Ye,Baoyan Guo,Jiemin Qiu,Jianle Zhuang,Chaofan Hu,Bingfu Lei,Guangqi Hu,Yingliang Liu
In this paper, a kind of novel fully UV absorbing carbon dots (R-CDs) were prepared as UV absorbers by a one-step hydrothermal method using natural renewable rutin as raw material. R-CDs can achieve multifunctional applications under acid-base amphoteric conditions. Under acidic conditions, R-CDs have high absorption efficiency (over 98%) in the whole ultraviolet region (200–400 nm) which have wider UV absorption range than commercial water-soluble organic UV absorbers (BP-4) and are almost completely transmitted in the visible light region. The UV absorption mechanism of R-CDs be investigated to the UV absorption systems of degradable cellulose coating (CMC) and the R-CDs@CMC coating was applied to prevent UV leakage of the 395UV chip. Under alkaline conditions, R-CDs remain fully UV absorbing and good absorption stability, but BP-4 loses its function as a UV absorber. Finally, R-CDs was applied to the UV absorption and anti-blue light systems of PU . The R-CDs@PU are more effective than commercially available anti-blue light lenses. At present, the preparation process of R-CDs is simple and rutin is a natural renewable energy source. So, R-CDs have the potential to become a new generation of water-soluble UV absorbers and be applied to prevent blue light.
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