PLANTA
·
2017
·Vol. 246
·期 2
·277-280
·
IF 3.361
Bashandy Hany,Teeri Teemu H.
Main conclusion
Unauthorized genetically engineered orange petunias were found on the market.
Genetic engineering of petunia was shown to lead to novel flower color some 20 years ago. Here we show that petunia lines with orange flowers, generated for scientific purposes, apparently found their way to petunia breeding programmes, intentionally or unintentionally. Today they are widely available, but have not been registered for commerce.
研究领域:
植物学
·
遗传学
✓ 已验证
DOI
PubMed
高影响因子
BMC PLANT BIOLOGY
·
2025
·Vol. 25
·期 1
·1-20
·
IF 4.8
Sun Meiyu,Han Baocai,Xiao Jianhua,Yu Haiyan,Jia Yinli,Yuan Shuhai,Yao Juan,Xing Quan,Wu Dongqi,Sun Guofeng,Zhang Jinzheng
Background Hemerocallis middendorffii is a kind of high quality ornamental plant with strong cold resistance, pleasant aroma, and gorgeous floral color. Hemerocallis is one of the three internationally recognized perennial flowers that has huge demand in the flower market. However, little is known about the floral scent and color biosynthesis in daylily due to the large genome size. Results We reported here a high-quality chromosome-scale genome of H. middendorffii which was a native daylily in China. Using PacBio HiFi and Hi-C sequencing technologies, we assembled a chromosome-level genome of 4.10 Gb with a contig N50 of 12.81 Mb. We identified 65,536 protein-coding genes across the 11 chromosomes. We employed a combination of genomic, transcriptomic, and metabolomic approaches to investigate mechanisms behind the floral scent and color biosynthesis in daylily. The main terpenoids contributing to floral fragrance were linalool, 3-carene, nerolidol, and α-farnesene, while carotenoids and anthocyanidins were the main components of floral color. These traits could be stably inherited by their hybrid progenies, and some of them exhibited heterosis. Through Weighted Gene Co-expression Network Analysis (WGCNA) analysis, we identified two modules MEblueviolet and MEgreenyellow related to terpenoids (floral scents) and carotenoids (floral colors) biosynthesis. The 10 hub genes including DXS , MVD , MVK , DXR , LCYBs , Z-ISO , CYPs , and JAS were screened by network construction. The results of qRT-PCR analysis showed that these hub genes were significant differentially expressed in daylily samples, and these results were basically consistent with the relative transcript levels of RNA-seq. Conclusions This study not only provides new insights into the molecular mechanisms of floral scent and color, but also lays a foundation for subsequent research on key gene functions and molecular breeding in Hemerocallis .
研究领域:
植物学
·
遗传学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Toxins
·
2025
·Vol. 17
·期 4
·181
·
IF 4
Qingling Zhan,Lina Liu,Wenjie Li,Jingshan Lu,Jiafu Jiang,Fadi Chen,Ye Liu,Zhiyong Guan
Alternariaspecies produce diverse secondary metabolites that act as critical virulence factors during plant pathogenesis. In cultivation areas ofChrysanthemum morifolium‘Fubai’—a key cultivar for herbal tea—black spot disease caused byA. alternatamanifests as necrotic leaf lesions progressing to wilting. Despite this disease’s economic impact, information on its associated toxins is limited, and the types of toxins produced by the black spot pathogen ofChrysanthemum morifolium‘Fubai’ in particular remain unclear. Furthermore, whether toxins are present in the flowers when the leaves show symptoms is uncertain, but their inflorescence is not visibly infected. Using two previously characterizedA. alternatastrains (F16/F20) isolated from ‘Fubai’ in earlier studies, we demonstrated the concomitant production of altenuene (ALT) and tenuazonic acid (TeA) in both strains, with strain-specific yield variations (F20 TeA: 342.16 µg/mL vs. F16: 21.84 µg/mL; ALT: 0.28 µg/mL vs. 0.90 µg/mL). Time-course monitoring revealed coordinated accumulation of both toxins in inoculated petals, reaching 18.07 μg/g ALT and 2.59 µg/g TeA by day 9. Notably, these two toxins were detected only in flower samples from black spot-infected plants, and their concentrations correlated closely with disease severity in the leaves. Moreover, although the inflorescences did not display symptoms, both fresh and dried flowers retained detectable toxin levels. We established a technical system for the extraction and quantitative detection of the toxins ALT and TeA produced by the black spot pathogen in tea chrysanthemum. This work provides the first confirmation of ALT/TeA co-contamination inChrysanthemum morifolium‘Fubai’, revealing substantial dietary exposure risks through tea consumption. Our findings suggest that, from a food safety risk reduction perspective, integrated management strategies should be developed to minimize toxin contamination in tea chrysanthemum, including improved disease prevention measures and potential regulatory considerations.
研究领域:
植物病理学
·
食品安全
·
次生代谢产物研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Wei Si,Lele Xiong,Han Zhou,Huimin Wu,Zhe Liu,Guoqiang Liu,Yanfang Liu,Aijin Shen,Xinmiao Liang
As a valuable medicinal and edible plant, C rocus sativus L. has had wide applications since ancient times. Herein, a comprehensive approach for characterization of constituents in saffron was established based on the combination of targeted and non-targeted strategies. A targeted UPLC-ESI/MS n strategy was applied for in-depth identification of crocins, and a non-targeted UPLC-ESI/MS 2 approach characterized other components. This integration strategy was used to analyze ingredients in 21 batches of saffrons from 6 origins. Forty-seven crocins belonging to 8 types were identified including 32 new crocins. Among them, 6 new compounds with specific structures were reported for the first time, i.e. trans -6(G, 2G), trans -4(GT, g), trans -3(GT), cis -3(GT), methyl ester- trans -2(G) and methyl ester- cis -2(G). Besides, 91 non-crocin components were identified including 43 new compounds. Based on systematic investigation of crocins and non-crocins, we found that crocins were the critical components to distinguish saffrons from different origins, especially between domestic and foreign samples.
研究领域:
药用植物学
·
分析化学
·
植物科学
✓ 已验证
DOI
PubMed
高影响因子
KEY ENGINEERING MATERIALS
·
2020
·
IF 0
Yi Peng Wang,Song Yang,Yun Zhen Jia,Pei Jian Sun,Xue Hui Sun,Hong Bo Wang,Jun Wei Guo,Xiao Bing Zhang,Hui Min Liu,Cong Nie
In this study, carboxymethyl peanut shell (CPS) was prepared, and then peanut shell-based porous material (PPS) was obtained by the complexation reaction of CPS with Cu2+. The carboxymethyl content of CPS increased gradually with the increase of the amounts of monochloroacetic acid and sodium hydroxide, and the Cu2+ loading amount of PPS increased gradually with the increase of carboxymethyl content. The scanning electron microscope results showed that PPS-1, PPS-2, PPS-3 (PPS-1~3) had many nanoscale pores. The nitrogen adsorption-desorption results suggested that PPS-1~3 were all mesoporous materials with an average pore diameter of about 10 nm, while their Brunauer-Emmet-Teller surface area and pore volume of PPS-1 to PPS-3 increased gradually. Compared with the control cigarette, the release of HCN in cigarette mainstream smoke could be effectively reduced with the use of PPS-1~3, the reduction rate of HCN per total particulate matter were 54.9%, 63.1% and 67.0%, respectively.
研究领域:
材料科学
·
环境科学
·
烟草研究
✓ 已验证
DOI
JOURNAL OF POLYMER ENGINEERING
·
2023
·Vol. 43
·期 3
·277-286
·
IF 2
Xiaoru Hao,Jun Xie,Yu Zhang,Wei Sheng,Haikun Zheng
This paper investigated anti-icing behavior and wettability of droplets on superhydrophobic polymercoatings between lotus effect and petal effect, which were prepared on surfaces of 2021 aluminum alloy with 1H, 1H, 2H, 2H-heptafluorodecyl (FAS-17). The prepared surfaces displayed excellent hydrophobicity with contact angles of 154.9° ± 1.5°and 139.8° ± 1.3°, while rolling angles are 4° ± 1.0° (lotus effect) and 30° ± 1.5° (petal effect). Thus, the present study focused on the different characterizations and the anti-icing potential of the superhydrophobic polymersurfaces were analyzed based on three parameters including the icing delay time, the crystallization temperature of water droplets, and contact time of impinging droplets on the cold superhydrophobic polymer coatings (−15 °C). Furthermore, the anti-icing of superhydrophobic coatings between lotus effect and petal effect with freezing time and crystallization temperature experimental phenomena were consistent with the thermodynamic analysis. It is also proved that the droplets have different bounce behavior on different polymercoating surfaces by droplet impact experiment. The study offers a comprehensive perspective on polymercoatings of different wetttablility for anti-icing behavior applications.
研究领域:
材料科学
·
表面工程
✓ 已验证
DOI
高影响因子
Frontiers in Pharmacology
·
2022
·
IF 5.988
Shi Le,Shu Yang,Hu Xiangdong,Akram Waheed,Wang Jun,Dong Shuang,Luo Biaobiao,Zhang Jiuliang,Hu Sheng,Li Xiaohua,Hu Xuebo
Chemotherapy is considered a most effective way to treat cancer. However, it is very common that chemotherapy causes unbearable mental and physical side effects to cancer patients, which ultimately reduces the patients’ confidence of overcoming diseases and compromises the treatment of chemotherapy. Cisplatin (DDP), a widely used anticancer agent for various types of cancers, also damages nontumor cells and tissues, which are mostly related to the activation of the inflammation pathway. Previously, we have discovered a few rational formulas of food as medicine materials that reduced systemic inflammation in in vitro and in vivo models. Hence, this study reports the ability of an optimized traditional Chinese anti-inflammatory formulation capable of synergizing the antitumor effect of DDP in vitro and in vivo. More significantly, by formulation of two anti-inflammatory herbal medicine, the Chrysanthemum × morifolium (Ramat.) Hemsl [Asteraceae] and Lonicera japonica Thunb [Caprifoliaceae] with a mediator Glycyrrhiza uralensis Fisch. ex DC [Fabaceae], a best formula relieved the kidney damage imposed by DDP. Treatments of various combinations of major chemical components of the three herbs also exhibited a similar trend for lowering the DDP-induced nephrotoxicity; however, contrary to that of the formula of herbal extracts, all chemical formulas could not recover the body weight and food intake of the tumor-bearing mice treated by DDP. Our findings suggested that the therapeutic index of DDP-based chemotherapy was able to be improved by minimizing toxicities from the two-herb formula to inhibit the inflammation in mouse tumor models and DDP-induced acute kidney injury mouse models.
研究领域:
肿瘤学
·
药理学
·
中医药学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2015
·
IF 2.912
Qian Lu,Qin Zhao,Qiong-Wei Yu,Yu-Qi Feng
In this study, a simple and convenient method for the determination of trans-resveratrol (TRA) in peanut oils based on pollen grain solid-phase extraction (SPE) was developed. Pollen grains were used as normal-phase SPE sorbent to separate TRA from peanut oils for the first time. As a naturally occurring material, pollen grains exhibited an excellent adsorption capacity for polyphenolic compounds due to their particular functional structures such as hydroxyl groups, saturated and unsaturated aliphatic chains with aromatics. Their stable compositions as well as adequate particle size (30–40 μm) also make them suitable for SPE. Several parameters influencing extraction performance were investigated. Coupled with high-performance liquid chromatography-ultraviolet detection (HPLC-UV), a green purification method for fast determination of TRA in peanut oils using pollen grain cartridges as sorbents was established. The linearity range of the proposed method was 10–2500 ng·g–1 with a satisfactory correlation coefficient (r2) of 0.9999. The limit of detection (LOD) for TRA in peanut oils was 2.7 ng·g–1, and the recoveries in spiked oil samples were from 70.2% to 98.4% with the relative standard deviations (RSDs) less than 4.9% (intraday) and 5.2% (interday). This method was successfully applied to the analysis of TRA in several peanut oils with different brands from local market as well as other kinds of vegetable oils.
研究领域:
食品科学
·
分析化学
✓ 已验证
DOI
高影响因子
ULTRASONICS SONOCHEMISTRY
·
2025
·
IF 9.7
Liwei Niu,Siwen Zhang,Xiaoyu Si,Yuhan Fang,Shuang Wang,Lulu Li,Zunlai Sheng
This study investigates the potential of ionic liquids (ILs) as sustainable solvents in ultrasonic-assisted extraction (UAE) to efficiently recover luteolin from peanut shells. Among the range of ILs tested, 1-butyl-3-methylimidazolium tetrafluoroborate stood out as the most effective solvent, achieving the highest extraction yield. Single-factor experiments were conducted to analyze the effects of ultrasonic power, extraction time, extraction temperature, IL concentration, and solid-to-liquid ratio on extraction efficiency. Further optimization of the extraction conditions was performed using response surface methodology and neural network analysis, resulting in a significantly enhanced luteolin yield of 3.71 ± 0.06 mg/g. Interaction energy analyses were conducted to elucidate the interactions between ILs and luteolin, confirming the experimental findings and highlighting the strongest interaction energy between 1-butyl-3-methylimidazolium tetrafluoroborate and luteolin. A kinetic model for luteolin extraction was developed, demonstrating that the extraction process follows a second-order rate model, where the extraction rate is directly proportional to the square of the concentration difference between luteolin and the solvent. The outcomes of this research present an efficient protocol for luteolin extraction and provide novel insights into the application of UAE in extracting natural products.
研究领域:
植物化学
·
天然产物开发
·
药用植物学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
·
2023
·Vol. 18
·期 3
·1734-1739
·
IF 3.4
Hao Lingchuan,Xu Wenxue,Wu Minghuo
Proanthocyanidins (PCs), known as natural antioxidants capable of scavenging free radicals, are rich in blueberry as oligomeric proanthocyanidins (OPCs). Aim to efficiently determine the total PCs in blueberry, a simple method of dispersive solid phase extraction (DSPE) followed by UV–Vis spectrophotometry detection was developed, in which C18-bonded silica microspheres were used as absorbent to purify the targets, and vanillin–HCl was applied for chromogenic reaction. After systematic optimization of DSPE conditions, reliable determination of total PCs was achieved. Though the sensitivity of this method was not comparable to those based on high performance liquid chromatography or mass spectrometry, it was simple and reliable for the analysis of PCs-rich fruits. With this method, impacts of storage conditions including time and temperature, as well as the freshness on total PCs concentration in blueberry were investigated. The results revealed that with the increase of storage time or temperature, the total PCs decreased accordingly. Meanwhile, the highest concentration of total PAs was found in the freshest blueberries stored at a low temperature of 4 °C, which supported that the low temperature was favorable for blueberry storage, showing the practical significance for the storage of PCs-rich fruits.
研究领域:
食品科学
·
分析化学
✓ 已验证
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2025
·
IF 6.2
Siwen Zhang,Liwei Niu,Xiaoyu Si,Lulu Li,Zunlai Sheng
Peanut shells, a byproduct of peanut cultivation, have limited uses but contain bioactive compounds like luteolin, a flavonoid with antioxidant, antibacterial, and anti-inflammatory properties. This study employed microwave-assisted extraction (MAE) technology combined with Natural Deep Eutectic Solvents (NADESs) to enhance the extraction process and clarify the underlying mechanisms. The most effective NADES, composed of choline chloride and ethylene glycol, was employed. Single-factor experiments assessed the effects of microwave time, power, and liquid-solid ratio on luteolin extraction. Following this, response surface methodology (RSM) and artificial neural network-genetic algorithm (ANN-GA) were applied for further optimization. The optimal extraction conditions were identified as 92 s of microwave time, 601 W of power, and a liquid-solid ratio of 32 mL/g, yielding a maximum luteolin extraction of 2.90 ± 0.02 mg/g. The ANN-GA and RSM models showed strong agreement with experimental values, with correlation percentages of 99.66 % and 98.95 %, respectively, indicating their reliability for optimizing extraction. Additionally, using the Conductor-like Screening Model for Real Solvents (COSMO-RS), quantum chemical calculations revealed that luteolin had the lowest logarithm of the infinite dilution activity coefficient (-16.43) and the most negative intermolecular interaction energy (-87.82 kJ/mol) in the NADES. These findings were consistent with validation test results, confirming the effectiveness of the NADES-MAE technology for luteolin extraction.
研究领域:
食品科学
·
化学工程
✓ 已验证
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Huilian Yu,Saif Ali,Ke Yan,Wenqing Zhao,Shanshan Wang,Zhiguo Zhou,Wei Hu
Drought stress negatively affects cotton pollen fertility, which in turn leads to a decrease in seed number per boll and boll weight. Exogenous melatonin application significantly enhances pollen fertility under drought conditions, while the specific underlying mechanisms remain unclear. A pot experiment was conducted using a cultivar Yuzaomian 9110 under two moisture treatments (soil relative water content at 75 ± 5 % and 45 ± 5 %) with two melatonin concentration (0 and 200 μM) to investigate the effects of exogenous melatonin on the structural traits and physiological metabolism of cotton anthers and its’ relationships with pollen fertility. Results demonstrated the significant impact of drought on anthers development and metabolism, with damage to the anther tapetum and decreased starch and adenosine triphosphate (ATP) contents, subsequently resulting in reduced pollen germination rate, seed number per boll and boll weight. Melatonin application in water-deficit anthers up-regulated the expression of sucrose transporter protein ( GhSWEET55 ) and phosphate sucrose synthetase, promoting sucrose import and synthesis, respectively. However, it also increased sucrose synthase and acid convertase, accelerating sucrose decomposition and reducing its content. Additionally, melatonin application promoted starch accumulation in water-deficit anthers by enhancing activities of adenosine diphosphate glucose pyrophosphorylase and soluble starch synthase, meaning that potential energy storage was increased, which facilitated the formation of pollen fertility. Although melatonin application reduced the expression of pyruvate kinase ( GhPK ) and glucose 6-phosphogluconate dehydrogenase ( GhG6PD ) genes in water-deficit anthers, it up-regulated hexokinase ( GhHXK ) and citrate synthase ( GhCIT ) expression, enhancing ATP content, and ultimately pollen fertility, seed number and boll weight under drought. In summary, exogenous melatonin preserved cotton pollen fertility under drought stress by regulating carbohydrate and energy metabolism, especially enhancing starch and ATP accumulation in anthers.
研究领域:
植物生理学
·
农业科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）