COLLOIDS AND SURFACES B-BIOINTERFACES
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2026
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IF 5.9
Runfeng Jia,Qingliang Chen,Khan Md Sabur,Zhongyu He,Bingxue Chen,Kaidi Hu,Xiaoqing Zhao,Hanbing Rao,Zhiwei Lu,Yanying Wang,Xing Wei,Mengmeng Sun
A single oral Lut-NPs/Gel system synergistically ameliorates both colitis and associated depression-like behaviors via the gut-brain axis. • Lut-NPs/Gel combats colitis via a synergistic combination of barrier repair, anti-inflammatory action, and beneficial microbiome remodeling. • Restoration of AKK abundance plays a critical regulatory role in gut microbiota remodeling. • Multi-omics analyses identified key modulated pathways, including TRP channels and neuroactive ligand-receptor interactions, functionally connecting gut repair to brain behavioral improvement. • Recovery of AKK in normal state is coordinated with upregulation of host repair genes (such as IGF1, Reg1), delineating a collaborative host-microbe network. Inflammatory bowel disease (IBD) is a chronic immune-mediated condition affecting the intestinal tract. Though conventional therapies aim to control symptoms and induce remission, their long-term use is hindered by systemic side effects and inadequate efficacy, highlighting the need for safer and more effective treatments. In this study, we developed luteolin-loaded nanoparticles (Lut-NPs) by encapsulating luteolin within metal-phenolic networks (MPNs) formed through coordination between epigallocatechin gallate (EGCG) and Ce 3 ⁺ ions. The resulting Lut-NPs showed excellent biocompatibility, strong antioxidant and anti-inflammatory activities, and remarkable stability under gastrointestinal conditions. To improve intestinal retention, the nanoparticles were incorporated into an inulin-based hydrogel, generating a Lut-NPs/Gel composite system. In a DSS-induced colitis model, oral administration of Lut-NPs/Gel demonstrated significant therapeutic efficacy, including restoration of the intestinal barrier, suppression of pro-inflammatory pathways, and reshaping the gut microbiome. The treatment also promoted macrophage polarization from the M1 to the M2 phenotype. AKK, usually considered beneficial but elevated during inflammation, was restored to normal levels after Lut-Nps/Gel treatment, suggesting its potential as a biomarker of intestinal recovery. Additionally, the treatment alleviated depression-like behaviors via the microbiota–gut–brain axis. Overall, this work provides a safe and promising multifunctional strategy for treating IBD and associated neuropsychiatric symptoms.
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Food Bioscience
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2026
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IF 6.2
Juntao Yu,Tonghao Du,Xingyu Zhu,Jiajia Zhang,Chengzhe Liu,Xiaobin Ruan,Xiaoxia Sun,Li Yang,Tao Xiong,Mingyong Xie,Tao Huang
Lacticaseibacillus paracasei NCU215 was recognized for its robust probiotic activities and excellent fermentation capabilities. In the present study, whole-genome, comparative genomics analysis, and experimental validation were integrated to systematically investigate the potential functional traits of NCU215. Genomic analysis revealed that NCU215 harbors a repertoire of functional genes and is devoid of genes associated with human pathogenicity. Additionally, the metabolic pathways involved in amino acid and carbohydrate metabolism in NCU215 were computationally predicted. Based on average nucleotide identity (ANI), average amino acid identity (AAI), and core-genome phylogenetic reconstruction, the taxonomic assignment of NCU215 was revised from Lacticaseibacillus casei to Lacticaseibacillus paracasei . Comparative genomic analysis indicated that NCU215 contains higher abundance of genes associated with cell adhesion, carbohydrate metabolism, and a unique amino acid metabolic gene cluster ( cysK–ctl1–cysE ). Experimental validation confirmed that NCU215 displays a high adhesion rate of 7.86 ± 0.65% to Caco-2 cells, the ability to convert methionine to cysteine, enhanced utilization of galactose, mannose, fructooligosaccharides, and inulin, as well as bile salt hydrolase activity and a high cholesterol-lowering efficacy of 47.30 ± 2.61%. Furthermore, soymilk fermented with NCU215 exhibited a substantial reduction in volatile compounds such as hexanal and 1-octen-3-ol, while the contents of acetoin and 2,3-butanedione were notably elevated.
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FOOD RESEARCH INTERNATIONAL
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2026
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IF 8.8
Yueyi Zhang,Shi Luo,Guodong Cao,Xin Song,Zhiqiang Xiong,Lianzhong Ai,Guangqiang Wang
D-allulose, erythritol, and sucralose showed distinct intestinal responses. • Sucralose showed the weakest support for beneficial bacterial growth in vitro. • Sucralose caused greater intestinal perturbation under inflammatory stress. • Sucralose-associated microbiota remodeling coincided with elevated p-cresyl sulfate. • p-cresyl sulfate altered inflammatory and barrier-related markers in Caco-2 cells. Sugar substitutes used in reduced-sugar foods differ in chemical structure, digestive fate, and microbial accessibility, yet their intestinal responses have rarely been compared under a common exposure framework. D-allulose, erythritol, and sucralose were evaluated as distinct sugar substitutes, with inulin included as a reference prebiotic carbohydrate. The ingredients were comparatively evaluated under a standardized exposure framework using in vitro fecal fermentation, healthy mice, and mice subjected to dextran sulfate sodium (DSS)-induced inflammatory stress. Gut microbiota profiling was integrated with inflammatory and barrier-related measurements, followed by serum metabolomics and Caco-2 validation of the sucralose-associated response. In vitro, D-allulose and inulin supported greater growth or enrichment of selected potentially beneficial bacteria, whereas sucralose showed the weakest growth support. In healthy mice, sucralose reduced microbial diversity and increased serum lipopolysaccharide by 23.2% relative to Control, accompanied by focal inflammatory cell infiltration and lower ZO-1 expression. Under DSS challenge, sucralose was associated with the greatest intestinal perturbation, including a 20.0% shorter colon than in the Model group, higher disease activity index and colonic myeloperoxidase levels, enhanced pro-inflammatory responses, and reduced tight-junction- and mucus-related markers. These changes coincided with enrichment of Faecalibaculum , depletion of Akkermansia and Dubosiella , and elevation of a serum feature annotated as p-cresyl sulfate. In Caco-2 cells, p-cresyl sulfate at 5 and 50 μM altered inflammation- and barrier-related markers without reducing cell viability. Overall, these findings demonstrate ingredient-specific intestinal response profiles and support considering gut microbial and intestinal effects alongside technological and nutritional attributes when evaluating sugar substitutes for reduced-sugar food applications. Download: Download high-res image (140KB) Download: Download full-size image
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Journal of Environmental Chemical Engineering
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2026
·Vol. 14
·期 5
·124471
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IF 7.5
Sijun Chen,Yang Liu,Jingrong Liu,Tingwei Zhang,Yongcan Jin,Huining Xiao
Sulfonated carbon materials are promising heterogeneous Brønsted acid catalysts for biomass valorization, but the facile construction of carbon frameworks with accessible acid sites remains challenging. Herein, a sulfonated fructose-based porous nanosheet carbon (SPC) was synthesized via a facile and energy-efficient two-step strategy involving P 2 O 5 -assisted rapid self-propagating carbonization and concentrated sulfuric acid sulfonation, and subsequently employed for fructose dehydration to HMF in a low-boiling 1,4-dioxane/H 2 O solvent system. During carbonization, P 2 O 5 simultaneously promoted fructose carbonization and directed the formation of a porous nanosheet architecture, avoiding conventional high-temperature activation processes. The resulting porous nanosheets provided abundant sites for -SO 3 H grafting and enhanced Brønsted acid site accessibility. Consequently, SPC exhibited efficient catalytic performance for the selective dehydration of fructose, affording an HMF yield of 70.1% at 140 ℃ for 30 min. Moreover, SPC efficiently converted concentrated fructose (1.0 g fructose) to HMF with a 59.4% yield and exhibited moderate recyclability. This work provides a facile strategy for constructing biomass-derived sulfonated carbon solid acids with effectively utilized Brønsted acid sites for biomass upgrading.
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Small Methods
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2025
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IF 9.1
Lin Cao,Jing Peng,Dengyi Duan,Haoran Cai,Qi Cao,Wenyi Zhang,Jinfeng Han,Kangkang Liu,Jianmin Li,Yuanjie Niu,Xuening Zhang,Yang Zhao,Gang Han
Inflammatory bowel disease is commonly treated with conventional therapies that suffer from poor drug bioavailability and significant side effects. This work develops a nanoparticle system using inulin, a naturally occurring polysaccharide, to address these challenges. This colon-targeted oral drug delivery system enables precise and efficient treatment of IBD by leveraging the colon-specific degradation of inulin by the enzyme inulinase. The nanoparticles demonstrate high stability in the upper gastrointestinal tract and ensure the release of the drug payload in the colon, triggered by inulinase activity. In mouse models, oral administration of these nanoparticles, both with and without drug loading, significantly reduce body weight loss, preserve colon length, and exhibit synergistic anti-inflammatory effects. Notably, even the nanoparticles without drug loading are effective in reducing inflammation and oxidative stress, likely due to the antioxidant properties of inulin. Additionally, the use of inulin promotes a beneficial shift in gut microbiota by decreasing pathogenic bacteria and increasing beneficial bacterial populations, further enhancing its therapeutic potential. These findings suggest that these prebiotic nanoparticles offer a targeted and effective strategy for IBD treatment by both anti-inflammatory benefits and microbiota modulation. This study highlights the potential of prebiotic-based nanomedicine for the treatment of IBD and possibly other colon-related diseases.
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PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
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2025
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IF 1.9
Shuhan Yang,Jing Jiang,Dan Zhao
Exopolysaccharide (EPS) from lactic acid bacteria is of interest for its multifunctional properties, but low yield limits industrial application. This study optimized EPS production by Weissella confusa P2 using response surface methodology (RSM). Plackett-Burman (PB) design identified significant factors (sucrose, sodium acetate, and K2HPO4), followed by steepest ascent approach to approximate optimal levels, with final optimization via central composite design (CCD). Under the optimized conditions (77.42 g/L sucrose, 3.42 g/L sodium acetate, 1.76 g/L K2HPO4, 12 g/L peptone, 12 g/L beef extract, 6 g/L yeast extract, 3 g/L ammonium citrate, 0.15%Tween-80, 0.2 g/L MgSO4 and initial pH value 6.5), EPS yield increased 1.60-fold to 49.93 g/L. W. confusa P2 EPS exhibited significant antioxidant activity, with scavenging rates of 40.2 ± 0.02% (DPPH), 30.2 ± 0.01% (ABTS), 45.6 ± 0.03% (·OH), and 40.7 ± 0.03% (H2O2), and a reducing power of 0.24 ± 0.03 (OD700 nm) at 5 mg/mL. Compared to commercial prebiotics, the EPS significantly stimulated the growth of probiotics, including Bifidobacterium lactis Bi-OA 07, Lactobacillus rhamnosus CICC 21773, and Lactobacillus casei HDS-01. Furthermore, the EPS induced a concentration-dependent coagulation in sucrose-supplemented skim milk. These results highlight the potential of W. confusa P2 EPS for food industry applications.
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Food and Bioprocess Technology
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2025
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IF 5.8
Huang Jie,Li Yu,Hu Jielun,Liu Huan
This study aimed to evaluate the effects of microwave treatment on the physicochemical properties of konjac glucomannan (KGM) and the prebiotic potential of KGM fractions with varying molecular weights (Mw). After microwave treatment, KGM exhibited a significant reduction in Mw which resulted in dramatic structural alterations including a transition to rod-like chain conformation and amorphous crystallinity, and fragmented surface morphology. The in vitro fermentation assay revealed that microwave-treated KGM fractions can be more effectively metabolized by gut microbiota, and the lower the molecular weight, the superior the fermentability. Strikingly, both total short-chain fatty acids (SCFA) and valeric acid in lower-Mw KGM fractions showed significantly higher concentrations than those in inulin. Microbiota diversity and composition analysis revealed that consumption of microwave-treated KGM significantly increased the relative abundance of beneficial gut microbiota, especially Firmicutes , Actinobacteria , and Lactobacillus , indicating enhanced prebiotic activity. Graphical
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2025
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IF 6.6
Nan Fang,Yan Zeng,Haosheng Huang,Xuemei Yang,Haiqin Leng,Xinyu Chen,Tao Chen,Lijun Zhou,Hongyu Yang,Xiaoyu Jiang,Chunbang Ding,Shiling Feng
Polysaccharides from Camellia oleifera seed cake are acknowledged as magnificent bioactivities, but their effects on gut microbiota are poorly studied. This investigation clarified the essential characteristics and bioactivities of four sorts of polysaccharides derived from C . oleifera seed cake (CCPs) and systematically investigated their digestive stability, microbial modulatory effects, and metabolic profiles under in vitro gastrointestinal and fermentation conditions. The findings provide an integrated understanding of how extraction methods influence polysaccharide functionality. The findings revealed that the fundamental traits and bioactivities of D-CCP would be impacted by the methods of extraction. Notably, D-CCP exhibited the highest antioxidant potential. Following in vitro digestion, both the physico-chemical characteristics and bioactivities of D-CCP remained largely consistent; however, significant alterations were noted after fermentation, affecting, reducing sugars, chemical composition, apparent viscosity, and FT-IR spectra. D-CCP played a beneficial role in regulating constitution of gut microorganisms by increasing the abundance of advantageous microorganisms ( Bacteroides, Parabacteroides and Bifidobacterium ) while reducing detrimental ones ( Morganella and Clostridium ), thereupon then promoting the manufacture of short-chain fatty acids. This result offers valuable insights into the characteristics of D-CCP related to both digestive processes and microbial fermentation, highlighting its promise as a prebiotic source that may enhance human intestinal health.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2025
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IF 3.5
Xiang Zhang,Jinfeng Bi,Youchuan Ma,Kaiyue Liu,Jianyong Yi
BACKGROUND Polysaccharides are increasingly being recognized as promising food-grade cryoprotectants due to their broad availability and health benefits. However, their practical application is hindered by limited ice recrystallization inhibition (IRI) activity. The purpose of this study was to compare the IRI activity of several natural polysaccharides. RESULTS We conducted a comparative analysis of molecular structures and cryoprotective properties using various physicochemical and structural characterization techniques. Among the polysaccharides studied, locust bean gum exhibited the highest IRI activity at 10 mg mL −1 , with mean largest grain size values of 51.5 μm. Inulin, citrus peel pectin, and locust bean gum demonstrated gradually increasing IRI activity as the concentration of the solution increased. This was likely due to enhanced formation of intermolecular networks that raised viscosity and created physical barriers that limited ice crystal growth. The correlation analysis revealed a significant negative correlation between the mean largest grain size of polysaccharides at a concentration of 1 mg mL −1 and 50% relative humidity. Melting temperature was significantly negatively correlated with O H, CO, and C O C, further confirming that the ability of polysaccharides to inhibit ice crystal growth is related to specific functional groups, relative humidity, and melting temperature. CONCLUSION The findings offer insights into the potential of polysaccharides to serve as effective natural cryoprotectants, improving the stability and texture of frozen foods. © 2025 Society of Chemical Industry.
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FOOD HYDROCOLLOIDS
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2025
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IF 12.4
Mengjun Wang,Shuo Zhang,Siyan Liu,Linyu Nian,Suilou Wang,Chongjiang Cao
Probiotics play a vital role in human health, but maintaining their high viability during processing and oral administration remains challenging. In this study, we utilized inulin and tannic acid (TA)/Ca 2+ metal-phenolic networks (MPNs) as coating materials to encapsulate anaerobic Bifidobacterium lactis (BL) using a layer-by-layer self-assembly strategy to improve their tolerance to the adverse gastrointestinal microenvironment and colonization in the intestinal tract. The results demonstrated that the three-dimensional gel coating formed by inulin could protect the MPNs coating from degradation by gastric acid and improve the ability of BL to resist gastric acid and bile salts, significantly increasing the survival rate of probiotics. Additionally, MPNs could effectively scavenge free radicals and resist cell damage caused by oxidative stress. Importantly, TA in MPNs could form strong interactions (hydrogen bonds) with mucin in the intestine, greatly prolonging the residence time of BL (more than 96 h). Moreover, the raw materials used to prepare BL@TA@Inulin are food-grade, thus ensuring high biosafety. In summary, BL@TA@Inulin represents a novel probiotic single-cell encapsulation approach to achieve highly active and adhesive delivery of probiotics, with potential applications in developing healthy foods and treating gastrointestinal tract-related diseases.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2026
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IF 8.7
Fengxian Luo,Xinchuang Wang,Tao Chen,Lihang Chen,Dan Zhang,Qingping Shi,Jinling Xu,Jiangning Hu
Inflammatory bowel disease (IBD) represents a chronic inflammatory disorder characterized by excessive reactive oxygen species (ROS) generation, persistent inflammatory response, and gut microbiota dysbiosis. Herein, a novel colon-targeted composite hydrogel (FTS/Gel) consisting of metal-polyphenol nanozymes (FTS) embedded in an inulin-based gel was developed for efficient oral treatment of IBD. This nanozyme (FTS) was synthesized by coordination between Fe 3+ ions and the anti-inflammatory drugs taxifolin (Tax) and L- Se -methylselenocysteine (SeMC), exhibiting remarkable superoxide dismutase (SOD)-like and catalase (CAT)-like activities. Moreover, FTS nanozymes significantly scavenged intracellular ROS and suppressed inflammation in RAW264.7 cells. Incorporating FTS into an inulin hydrogel notably enhanced colon-targeting efficiency, bio-adhesion, and retention time in vivo. Oral administration of FTS/Gel effectively alleviated colitis, as evidenced by increased colon length, reduced splenomegaly, restored intestinal epithelial barrier integrity, and enhanced mucin secretion. Furthermore, FTS/Gel treatment rebalanced gut microbiota composition by promoting beneficial bacteria and suppressing pernicious bacteria. Collectively, this study presents a promising therapeutic strategy combining antioxidant nanozymes and prebiotic-based hydrogel delivery systems for effectively managing and treating IBD.
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BIOCHEMICAL ENGINEERING JOURNAL
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2023
·
IF 3.9
Zhi-tao Li,Yu-ying Wang,Hang-yan Ji,Yun Jiang,Min-jie Gao,Xiao-bei Zhan,Zhengyu Jin
Nowadays, research on the influence of food nutrition and medicine on the metabolism and diversity of the human gut microbiota is of considerable interest. In this study, a new in-vitro simulation colon reactor (SCR), an important tool for simulating the colon conditions of the human body, was designed to evaluate food function. The key parameters of crush evaluation, peristaltic patterns, anaerobic and parthenogenic aerobic bacterial growth, and fecal microbiota colonization were analyzed and compared with those of static reactor to evaluate colonization and the effectiveness of the new bioreactor . The results prove that SCR has a better ability to break up semisolids, and it could be used to simulate the digestion of semisolid foods. It could also successfully simulate two classical human gut peristaltic patterns, with the intestinal pressure reaching 60–90 (peristaltic frequency, six per min) and 100–150 mmHg (peristaltic frequency, 10 per min). SCR is suitable for fermentation colonization culture of Akkermansia muciniphila , Bifidobacteria , and fecal microbiota. The gut microbiota could be maintained in a healthy and stable growth state throughout the fermentation process. SCR could also increase the productivity of gut microbiota, and it has a good effect on fecal colonization. This study provides a novel and valuable tool for evaluating the structure and function of the gut microbiota.
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危险品化学品经营许可证（带存储）